Article
Biotechnology & Applied Microbiology
Jatziri Mota-Gutierrez, Lukas Lis, Amaia Lasagabaster, Ibai Nafarrate, Ilario Ferrocino, Luca Cocolin, Kalliopi Rantsiou
Summary: This study discusses the microbial ecology of the broiler gut environment and the prevalence of Campylobacter across the broiler production chain. It suggests the use of bacteriophages as a possible mitigation strategy. Results show that the pathogen load is likely to increase at different stages of the production chain, with water and feed contamination being the main sources during primary production. Cross-contamination occurs during slaughter, dressing, and processing processes. Knowledge gaps include the lack of studies detecting Campylobacter in broilers in European countries and the bacterial load in crates used for transportation. Determining the prevalence of Campylobacter in the broiler industry is essential for producing low-risk broiler flocks and meat products.
Article
Virology
Xinxin Jin, Xiuxiu Sun, Zui Wang, Junfeng Dou, Zhengdan Lin, Qin Lu, Tengfei Zhang, Guoyuan Wen, Huabin Shao, Guofu Cheng, Qingping Luo
Summary: The phage WH1 isolated from chicken feces is a novel virus in the Siphoviridae family, and it shows good antibacterial activity against E. faecalis. The results suggest that it can be a potential biocontrol agent to reduce the formation of E. faecalis biofilm and also be used as an alternative for the control of E. faecalis in chicken products.
Article
Food Science & Technology
Ozgul Aydin Demirarslan, Hamza Alasalvar, Zeliha Yildirim
Summary: The study showed that SE-phages can effectively reduce the number of Salmonella in chicken meat and skin, even to undetectable levels during storage. Additionally, SE-phages can also prevent subsequent Salmonella contamination at 4 degrees Celsius.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Microbiology
Luis Tanoeiro, Monica Oleastro, Alexandra Nunes, Andreia T. Marques, Silvia Vaz Duarte, Joao Paulo Gomes, Antonio Pedro Alves Matos, Jorge M. B. Vitor, Filipa F. Vale
Summary: This study identified two novel prophages in Campylobacter coli and explored their role in gene transfer and evolution between species. The presence of multiple prophages in Campylobacter spp. populations, with different distribution patterns in human and non-human isolates, suggests their involvement in evolutionary introgression events.
Article
Microbiology
Ayidh Almansour, Ying Fu, Tahrir Alenezi, Mohit Bansal, Bilal Alrubaye, Hong Wang, Xiaolun Sun
Summary: This study revealed that microbiota from specific pathogen-free mice can successfully colonize the chicken gut and reduce Campylobacter jejuni colonization in chickens. The introduction of microbiota also altered the composition of bacterial phyla, inhibiting the growth of C. jejuni and providing potential strategies for reducing chicken contamination and campylobacteriosis.
Article
Food Science & Technology
Hoang Minh Duc, Yu Zhang, Hoang Minh Son, Hung-Hsin Huang, Yoshimitsu Masuda, Ken-ichi Honjoh, Takahisa Miyamoto
Summary: In this study, a lytic phage STG2 capable of reducing S. enteritidis and S. typhimurium contaminations on cabbage and various surfaces was characterized. Genomic characterization revealed that phage STG2 does not contain any genes associated to antibiotic resistance, toxins, lysogeny, or virulence factors. Additionally, phage STG2 exhibited great efficacy in reducing both planktonic cells and biofilms on different surfaces.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Immunology
Mengfei Peng, Jungsoo Joo, Zabdiel Alvarado-Martinez, Zajeba Tabashsum, Arpita Aditya, Debabrata Biswas
Summary: This study developed an intracellular autolytic Salmonella enterica (SE) vaccine and applied it to a poultry model. It was found that the vaccine significantly reduced intracellular viability, enhanced immune response, reduced SE intestinal colonization, and modulated intestinal microbial composition.
Review
Food Science & Technology
Haojie Ge, Shasha Fu, Huimin Guo, Maozhi Hu, Zhengzhong Xu, Xiaohui Zhou, Xiang Chen, Xin'an Jiao
Summary: In recent years, there has been an increase in foodborne diseases caused by pathogens. Bacteriophages (phages) have the potential to play a significant role in preventing, controlling, and treating these diseases due to their advantages such as inhibiting pathogenic bacteria and prolonging the shelf life of food. However, there are still challenges in the practical application of phages in the food industry.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Review
Food Science & Technology
Justin Falardeau, Aljosa Trmcic, Siyun Wang
Summary: Listeria monocytogenes poses a food safety risk in ready-to-eat foods like fresh and soft/semisoft cheeses. Researchers have investigated various biocontrol strategies such as bacteriocins, bacteriophages, and competition with native microbiota to inhibit the growth of L. monocytogenes in susceptible cheeses. While these strategies have shown success in reducing L. monocytogenes populations in cheeses, regrowth of surviving cells remains a common issue.
COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY
(2021)
Article
Veterinary Sciences
Napakhwan Imklin, Pattaraporn Sriprasong, Sahathat Phuttapatimok, Tanyanant Kaminsonsakul, Yonlayong Woonwong, Pichai Jirawattanapong, Porntippa Lekcharoensuk, Narut Thanantong, Rujikan Nasanit
Summary: In this study, 6 phages were isolated and tested for their efficacy against E. coli strains isolated from diarrheal pigs. Phage therapy was found to significantly reduce fecal E. coli counts and restore the gut's bacterial composition. Additionally, giving phage cocktails resulted in higher average daily gain in piglets.
RESEARCH IN VETERINARY SCIENCE
(2022)
Article
Infectious Diseases
Tengteng Li, Hong Chen, Jiayi Zhao, Zhenxiang Tao, Weiqing Lan, Yong Zhao, Xiaohong Sun
Summary: In this study, a novel Salmonella phage vB_SalM_SPJ41 was isolated from poultry farms in Shanghai, China. It was found that this phage could lyse multiple serotypes of antibiotic-resistant S. enterica and effectively inhibit the biofilm produced by antibiotic-resistant S. enterica. Additionally, the phage showed promising results in reducing the levels of S. Enteritidis and S. Derby in lettuce and salmon at low temperatures. Genomic analysis revealed the absence of virulence factor genes or antibiotic resistance genes in the phage.
Article
Microbiology
Ahmed Esmael, Ehab Azab, Adil A. Gobouri, Mohamed A. Nasr-Eldin, Mahmoud M. A. Moustafa, Shereen A. Mohamed, Omnia A. M. Badr, Alzahraa M. Abdelatty
Summary: Foodborne salmonellosis is a global threat, and in this study, two broad-spectrum lytic Salmonella phages, SPHG1 and SPHG3, were isolated and characterized for their ability to infect multidrug-resistant Salmonella Typhimurium. These phages showed strong lytic activity with a short lysis time and high burst sizes, and were stable under various environmental conditions. A phage cocktail containing SPHG1 and SPHG3 significantly reduced the viable count of S. Typhimurium in milk, water, and chicken breast, demonstrating their potential as biocontrol agents against foodborne salmonellosis.
Article
Food Science & Technology
Fei Ma, Yu Ning, Qingqing Wan, Likou Zou, Yuntao Liu, Shujuan Chen, Jianlong Li, Zhen Zeng, Yong Yang, Hong Chen, Min Zhou, Shuliang Liu, Cheng Li, Aiping Liu
Summary: Bacteriophages LSA2308 and LSA2366 showed lytic activity against drug-resistant S. aureus, and combined with ampicillin achieved better clearance in LB broth. In skim milk, a concentration of 10^7 PFU/mL bacteriophages exhibited the most effective bactericidal efficacy against S. aureus strains.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Microbiology
Kishani N. Naligama, Anupama P. Halmillawewa
Summary: Pectobacterium carotovorum phage vB_PcaM_P7_Pc (P7_Pc) isolated from infected carrot samples in Sri Lanka was comprehensively characterized and classified under the genus Certrevirus. This study provides a foundation and theoretical basis for the application of P7_Pc as a biocontrol agent.
MICROBIOLOGY SPECTRUM
(2022)
Review
Pharmacology & Pharmacy
Yu Zhang, Chu-Xin Li, Xian-Zheng Zhang
Summary: Symbiotic microbiota is considered an invisible organ of the human body, with its imbalance linked to various diseases. Phage therapy, with high selectivity for specific bacteria, has shown potential in precisely modulating microbiota and treating diseases successfully.
ADVANCED DRUG DELIVERY REVIEWS
(2021)
Article
Microbiology
Geraldine M. Flaujac Lafontaine, Philip J. Richards, Phillippa L. Connerton, Peter M. O'Kane, Nacheervan M. Ghaffar, Nicola J. Cummings, Neville M. Fish, Ian F. Connerton
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Microbiology
Philip J. Richards, Geraldine M. Flaujac Lafontaine, Phillippa L. Connerton, Lu Liang, Karishma Asiani, Neville M. Fish, Ian F. Connerton
Review
Microbiology
Md. Sharifull Islam, Yang Zhou, Lu Liang, Ishatur Nime, Ting Yan, Stephan P. Willias, Md. Zakaria Mia, Weicheng Bei, Ian F. Connerton, Vincent A. Fischetti, Jinquan Li
Article
Microbiology
Lu Liang, Nicholas B. Carrigy, Samuel Kariuki, Peter Muturi, Robert Onsare, Tobi Nagel, Reinhard Vehring, Phillippa L. Connerton, Ian E. Connerton
Article
Virology
Ahmed Mohamed, Omar Taha, Hesham M. El-Sherif, Phillippa L. Connerton, Steven P. T. Hooton, Nabil D. Bassim, Ian F. Connerton, Ayman El-Shibiny
Article
Nutrition & Dietetics
Geraldine M. Flaujac Lafontaine, Neville M. Fish, Ian F. Connerton
Article
Microbiology
Helene N. Chinivasagam, Wiyada Estella, Lance Maddock, David G. Mayer, Caitlin Weyand, Phillippa L. Connerton, Ian F. Connerton
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Microbiology
Ting Yan, Lu Liang, Ping Yin, Yang Zhou, Ashraf Mahdy Sharoba, Qun Lu, Xingxing Dong, Kun Liu, Ian F. Connerton, Jinquan Li
Article
Biochemistry & Molecular Biology
Fanqiang Meng, Fengxia Lu, Hechao Du, Ting Nie, Xiaoyu Zhu, Ian F. Connerton, Haizhen Zhao, Xiaomei Bie, Chong Zhang, Zhaoxin Lu, Yingjian Lu
Summary: The synthesis of plantaricin in Lactobacillus plantarum is regulated by quorum sensing, with the auto-inducing peptide PlnA1 and acetate identified as key regulators. PlnA1 activates quorum sensing in log phase growth, while acetate activates it in stationary phase, maintaining the synthesis of plantaricin under conditions of reduced growth. This research offers insights into the developmental regulation of plantaricin synthesis and has potential applications in food fermentation and bacteriocin production.
MOLECULAR MICROBIOLOGY
(2021)
Article
Microbiology
Mohamed El-Telbany, Gamal El-Didamony, Ahmed Askora, Eman Ariny, Dalia Abdallah, Ian F. Connerton, Ayman El-Shibiny
Summary: Phage therapy offers a promising alternative to antibiotics to combat multi-drug resistant bacteria. The isolated phage vB_ZEFP showed potential in controlling the growth of Enterococcus faecalis, especially in preventing infection after root canal treatment.
Article
Virology
Daniela D'Angelantonio, Silvia Scattolini, Arianna Boni, Diana Neri, Gabriella Di Serafino, Philippa Connerton, Ian Connerton, Francesco Pomilio, Elisabetta Di Giannatale, Giacomo Migliorati, Giuseppe Aprea
Summary: The study assessed the efficacy of two field-isolated bacteriophages against an antimicrobial-resistant Campylobacter jejuni strain in experimental infections. A two-step phage application successfully reduced the Campylobacter load in broilers, showcasing the potential of phage therapy in controlling Campylobacter in poultry.
Article
Multidisciplinary Sciences
Ali Alrasheed, Phillippa Connerton, Ghedeir Alshammari, Ian Connerton
Summary: The research critically analyzed the food safety and hygiene knowledge in Saudi Arabia, finding that while education levels were high, there were still deficiencies in understanding of HACCP; improvements are needed in food safety training for all levels of staff, and accessibility of food policies should be enhanced.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2021)
Article
Multidisciplinary Sciences
Ahmed Saeedi, Nicola J. Cummings, Denise McLean, Ian F. Connerton, Phillippa L. Connerton
Summary: ASxL5(T) is a novel Gram-stain negative bacterium with unique growth characteristics and morphology, closely related to members of the Oceanospirillaceae.
SCIENTIFIC REPORTS
(2021)
Article
Agriculture, Dairy & Animal Science
Adam Lee, Stephen C. C. Mansbridge, Lu Liang, Ian F. F. Connerton, Kenneth H. H. Mellits
Summary: New-born piglets that fail to thrive during suckling may suffer from lack of nutrition due to excessive piglet production values. The use of commercial milk replacers can provide nutrition but lacks milk sugars that contribute to immune system and gut microbiota development. This study investigated the effects of supplementing milk replacer with milk sugars on gut health, microbiome, and immune-protective goblet cells in piglets. The results showed that milk replacers supplemented with milk sugars improved gut health, increased beneficial gut bacteria, and enhanced the numbers of protective immune goblet cells. This suggests that milk sugars in milk replacers can play a beneficial role in the husbandry of poorly performing piglets moved to controlled environments.
Article
Veterinary Sciences
Adam Lee, Lu Liang, Phillippa L. Connerton, Ian F. Connerton, Kenneth H. Mellits
Summary: This study aimed to determine if dietary galacto-oligosaccharide supplementation during gestation could improve immunity, reduce rotavirus infection, and modulate the microbiota in sows and neonates. The results showed that galacto-oligosaccharide supplementation during gestation significantly increased rotavirus-specific IgG and IgA in sow colostrum, thereby reducing neonatal rotavirus infection and suppressing potential pathogenic bacteria.
FRONTIERS IN VETERINARY SCIENCE
(2023)