Article
Biochemistry & Molecular Biology
Veronika Smolinska, Michaela Debreova, Martina Culenova, Maria Csobonyeiova, Andrey Svec, Lubos Danisovic
Summary: Healing of articular cartilage defects is challenging and traditional reparative approaches are limited. Mesenchymal stromal cells have been extensively studied and proven to have therapeutic effects on articular cartilage.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Medicine, Research & Experimental
Wei Pan, Hongyuan Chen, Aijun Wang, Fengshan Wang, Xinke Zhang
Summary: Exosomes are small membrane vesicles secreted by most cell types, transmitting bioactive elements to affect the gene expression and function of recipient cells. Mesenchymal stem cells (MSCs) have been confirmed to be promising in tissue repair and regeneration, and exosomes secreted by MSCs have shown similar therapeutic effects. However, the inefficient production has hindered their clinical development. This review summarizes potential methods to efficiently promote the yield of exosomes, including altering the process of exosome biogenesis and secretion, cell culture conditions, cell expansion through 3D dynamic culture, and isolation of exosomes. The application of MSCs-derived exosomes in disease treatment is also discussed.
Article
Engineering, Biomedical
Shuangpeng Jiang, Guangzhao Tian, Zhen Yang, Xiang Gao, Fuxin Wang, Juntan Li, Zhuang Tian, Bo Huang, Fu Wei, Xinyu Sang, Liuqi Shao, Jian Zhou, Zhenyong Wang, Shuyun Liu, Xiang Sui, Quanyi Guo, Weimin Guo, Xu Li
Summary: This study confirms that hWJMSC-Exos can enhance the effect of the ACECM scaffold and promote osteochondral regeneration. The promoting effect may be related to the polarization of macrophages and inhibition of inflammatory response by hWJMSC-Exos. Additionally, hWJMSC-Exos contain miRNAs that can promote hyaline cartilage regeneration.
BIOACTIVE MATERIALS
(2021)
Review
Biochemistry & Molecular Biology
Yi Ding, Qiulin Luo, Hanyun Que, Nan Wang, Puyang Gong, Jian Gu
Summary: Liver disease is a major global health and economic burden with limited treatment options. MSC-derived exosomes have shown promising potential as a new treatment strategy for liver diseases by promoting hepatocyte proliferation and repair. This paper reviews the physiological functions of exosomes and discusses their advantages as drug delivery vehicles, as well as the challenges in future exosome treatment of liver diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Medicine, Research & Experimental
Li Jiang, Jingwei Lu, Yixuan Chen, Kexin Lyu, Longhai Long, Xiaoqiang Wang, Tianzhu Liu, Sen Li
Summary: Tendon injury is a common disorder caused by overuse or trauma. Mesenchymal stem cells (MSCs), known for their proliferative capacity and ability to regenerate, have shown promise in treating various diseases including tendon injury. They can differentiate into specific cells and secrete biologically active molecules to promote tissue repair and regeneration.
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
(2023)
Article
Medicine, Research & Experimental
Jing-Yuan Cao, Bin Wang, Tao-Tao Tang, Yi Wen, Zuo-Lin Li, Song-Tao Feng, Min Wu, Dan Liu, Di Yin, Kun-Ling Ma, Ri-Ning Tang, Qiu-Li Wu, Hui-Yao Lan, Lin-Li Lv, Bi-Cheng Liu
Summary: In this study, MSC-exos were found to preferentially target injured kidneys and localize to proximal tubules in ischemic AKI. The researchers demonstrated that MSC-exos ameliorated ischemic AKI and promoted tubular repair by targeting the cell cycle arrest and apoptosis of TECs through the miR-125b-5p/p53 pathway. This study provides new insights into the role of MSC-exos in renal tubule repair and highlights their potential as a promising therapeutic strategy for AKI.
Article
Cell Biology
Qingju Li, Yinchuan Xu, Kaiqi Lv, Yingchao Wang, Zhiwei Zhong, Changchen Xiao, Keyang Zhu, Cheng Ni, Kan Wang, Minjian Kong, Xuebiao Li, Youqi Fan, Fengjiang Zhang, Qi Chen, Yi Li, Qian Li, Chengjia Liu, Jinyun Zhu, Shuhan Zhong, Jingyi Wang, Yongjian Chen, Jing Zhao, Dan Zhu, Rongrong Wu, Jinghai Chen, Wei Zhu, Hong Yu, Reza Ardehali, Jianyi (Jay) Zhang, Jian'an Wang, Xinyang Hu
Summary: This study highlights the key role of sEV miR-486-5p in promoting cardiac angiogenesis. miR-486-5p acts on the fibroblastic MMP19-VEGFA signaling pathway to promote cardiac recovery. Delivery of miR-486-5p-engineered sEVs safely enhanced angiogenesis and cardiac function in a nonhuman primate (NHP) MI model, potentially promoting cardiac repair.
SCIENCE TRANSLATIONAL MEDICINE
(2021)
Review
Cell & Tissue Engineering
Jiaxi Liu, Jinfang Gao, Zixie Liang, Chong Gao, Qing Niu, Fengping Wu, Liyun Zhang
Summary: This review discusses the characteristics of MSCs, their interaction with the microenvironment, and the importance of the microenvironment in maintaining cell function and disease progression.
STEM CELL RESEARCH & THERAPY
(2022)
Review
Immunology
Niki Chalkidi, Christina Paraskeva, Vasiliki Koliaraki
Summary: The mammalian intestine is a self-renewing tissue that relies on various cell types such as intestinal epithelial cells, stem cells, immune cells, and stromal cells to maintain its homeostasis. When the mucosal homeostasis is disrupted due to injury or inflammation, a series of events are triggered, leading to tissue repair and restoration of organ function. Fibroblasts play significant roles in maintaining epithelial and immune homeostasis, as well as responding to tissue damage in the intestine through secretion of soluble mediators and remodeling of extracellular matrix. The heterogeneity of fibroblasts in both normal and inflammatory conditions suggests diverse functions, but there is still limited understanding on the number, terminology, and functional properties of these subsets.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Basanta Bhujel, Se-Heon Oh, Chang-Min Kim, Ye-Ji Yoon, Young-Jae Kim, Ho-Seok Chung, Eun-Ah Ye, Hun Lee, Jae-Yong Kim
Summary: The cornea is susceptible to damage, but corneal transplantation can restore its functions. However, this procedure faces obstacles such as a shortage of donors and graft rejection. Mesenchymal stem cells (MSCs) have shown potential in corneal regeneration by differentiating into corneal cells and secreting growth factors. This review highlights the contribution of MSCs and MSC-derived exosomes in overcoming corneal disease and their potential as therapeutic agents.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
David A. Hart, Norimasa Nakamura
Summary: After injuries to musculoskeletal tissues, an inflammatory response occurs to promote endogenous repair, but it often results in functionally inferior scar tissue. Injuries in adults no longer have the anabolic processes that contribute to growth and maturation, which can lead to degenerative conditions such as osteoarthritis. Researchers have been exploring cellular therapies, such as Platelet-rich Plasma and mesenchymal stem/medicinal signaling cells, to enhance tissue repair and regeneration. However, implanting these cells or engineered constructs into an inflammatory adult environment may compromise their potential for regeneration. Therefore, it may be necessary to neutralize the inflammatory environment and create an anabolic environment for optimal outcomes.
Review
Immunology
Maedeh Arabpour, Amene Saghazadeh, Nima Rezaei
Summary: MSC-derived exosomes have the potential to polarize M2 macrophages and modulate inflammation in various diseases and conditions like CNS diseases, autoimmune diseases, inflammatory bowel disease, and cardiomyopathy.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Review
Immunology
Weiqiang Li, Qianqian Liu, Jinchao Shi, Xiang Xu, Jinyi Xu
Summary: Mesenchymal stem cells (MSCs) are pluripotent stem cells with strong immunomodulatory capacity. They rely on microenvironmental factors to stimulate specific cell growth factors, chemokines, immunomodulatory factors, or receptors, or to direct their differentiation into specific tissue cells for tissue regeneration and repair and immunomodulatory functions.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Liming Li, Jiafu Mu, Yu Zhang, Chenyang Zhang, Teng Ma, Lu Chen, Tianchen Huang, Jiahe Wu, Jian Cao, Shiqing Feng, Youzhi Cai, Min Han, Jianqing Gao
Summary: This study found that exosomes derived from hypoxic preconditioned human umbilical vein endothelial cells can stimulate angiogenic MSCs, showing great potential in the treatment of traumatic spinal cord injury.
Article
Multidisciplinary Sciences
Bruno Aristides Bronel, Edgar Maquigussa, Mirian Aparecida Boim, Antonio da Silva Novaes
Summary: This study evaluated the potential beneficial effects of iPSC-derived EV-iPSCs in an in vitro fibrosis model using mouse mesangial cells (MMCs). The results showed that EV-iPSCs effectively reduced inflammation, suppressed RAS activation, and mitigated fibrogenesis.
SCIENTIFIC REPORTS
(2023)
Review
Chemistry, Multidisciplinary
Xu Zhang, Hongbo Zhang, Jianmei Gu, Jiayin Zhang, Hui Shi, Hui Qian, Dongqing Wang, Wenrong Xu, Jianming Pan, Helder A. Santos
Summary: Extracellular vesicles (EVs) have shown great potential as a cell-free strategy for cancer therapy, with the ability to be modified and engineered to enhance their therapeutic efficiency and safety. Recent advances have focused on strategies to scale up production, improve cargo-loading, and enhance tumor-targeting of engineered EVs.
ADVANCED MATERIALS
(2021)
Review
Engineering, Biomedical
Peipei Wu, Bin Zhang, Dickson Kofi Wiredu Ocansey, Wenrong Xu, Hui Qian
Summary: Extracellular vesicles play a crucial role in disease treatment and diagnosis, with engineered design enhancing their therapeutic capability and diagnostic imaging potential.
Article
Cell Biology
Xinye Han, Peipei Wu, Linli Li, Hassan Mohamud Sahal, Cheng Ji, Jiahui Zhang, Yi Wang, Qichen Wang, Hui Qian, Hui Shi, Wenrong Xu
Summary: DF-Ex can reverse the damage caused by high glucose in HUVECs and accelerate diabetic cutaneous wound healing; DF-Ex exerts its effects by activating the Akt/beta-catenin pathway; This research provides a new treatment strategy for diabetic cutaneous wound healing.
Article
Cell Biology
Yan Wang, Huiting Wang, Ruiqi Zheng, Peipei Wu, Zixuan Sun, Jingyan Chen, Leilei Zhang, Chenxiao Zhang, Hui Qian, Jiajia Jiang, Wenrong Xu
Summary: In this study, circ-ITCH was found to suppress GC metastasis by acting as a sponge for miR-199a-5p and increasing Klotho expression, providing potential biomarkers and targets for the diagnosis and therapy of GC.
Review
Cell & Tissue Engineering
Qian Jin, Peipei Wu, Xinru Zhou, Hui Qian, Wenrong Xu
Summary: Exosomes, small extracellular vesicles secreted by cells, play crucial roles in intercellular communication in the CNS, contributing to CNS development and homeostasis as well as potentially influencing the progression of neurological disorders. Additionally, exosomes may serve as diagnostic biomarkers and therapeutic tools for these disorders, showing promise in the field of neurological research.
STEM CELLS INTERNATIONAL
(2021)
Article
Oncology
Hui Shi, Yaoxiang Sun, Hongru Ruan, Cheng Ji, Jiahui Zhang, Peipei Wu, Linli Li, Chihan Huang, Yuanwang Jia, Xu Zhang, Wenrong Xu, Jiajia Jiang, Hui Qian
Summary: The study found that the anti-cancer drug DIM at therapeutic concentrations upregulated the expression of tumor-related factors in GC-MSCs and promoted the proliferation, invasion, and migration of gastric cancer cells, leading to tumor growth. This mechanism provides a new insight into anti-cancer drug resistance and suggests the need to adjust the chemotherapy regimen to minimize the side effects of drugs on targeted TME cells.
FRONTIERS IN ONCOLOGY
(2021)
Letter
Oncology
Zhao Ting, Zhi-xin Yan, You-wen Tan, Fu-ji Yang, Sun Hui, Mao Fei, Zhu Wei, Wen-rong Xu, Qian Hui, Yong-min Yan
CLINICAL AND TRANSLATIONAL MEDICINE
(2021)
Review
Immunology
Xinwei Xu, Jintu Huang, Dickson Kofi Wiredu Ocansey, Yuxuan Xia, Zihan Zhao, Zhiwei Xu, Yongmin Yan, Xu Zhang, Fei Mao
Summary: Methylation is a significant epigenetic modification found in both DNAs and RNAs, playing crucial roles in various cancers, especially colorectal cancer. The m6A modification regulates RNA and immune cells, affecting intestinal mucosal immunity and the development of inflammatory bowel disease and colorectal cancer. m6A-related genes and factors may serve as potential predictive markers and therapeutic targets.
JOURNAL OF INFLAMMATION RESEARCH
(2021)
Review
Medicine, Research & Experimental
Yifei Chen, Fuji Yang, Ying Chu, Zhihua Yun, Yongmin Yan, Jianhua Jin
Summary: Metabolic diseases, including obesity, diabetes, and nonalcoholic fatty liver disease, are increasing in incidence and prevalence, posing a major health and socioeconomic burden globally. While there is growing understanding of these diseases, effective treatments remain a challenge. Recent research suggests that mitochondrial dysfunction plays a role in the pathogenesis of metabolic diseases, and the use of healthy mitochondria as a therapeutic approach shows promise.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Review
Pharmacology & Pharmacy
Benshuai You, Yang Yang, Zixuan Zhou, Yongmin Yan, Leilei Zhang, Jianhua Jin, Hui Qian
Summary: EVs have shown cardioprotective effects in heart disease by activating beneficial signaling pathways. Engineering approaches have been developed to improve the therapeutic efficiency of EVs, but further optimization is needed to achieve better cardiac repair outcomes.
Review
Pharmacology & Pharmacy
Fengtian Sun, Yuntong Sun, Feng Wu, Wenrong Xu, Hui Qian
Summary: This review introduces the novel cell-free strategy of mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) and their applications in the treatment of diabetes mellitus (DM) and diabetic complications. The major characteristics of MSC-EVs are summarized, and the recent advances in the use of engineered MSC-EVs for treating hyperglycemia-induced tissue damage are discussed. Additionally, the potential applications of engineered MSC-EVs in the therapy of DM-related diseases are presented, and the opportunities and challenges for clinical translation of MSC-EVs, particularly engineered MSC-EVs, are evaluated.
Article
Cell & Tissue Engineering
Yifei Wang, Yaqin Zhang, Bing Lu, Jianbo Xi, Dickson Kofi Wiredu Ocansey, Fei Mao, Donglin Hao, Yongmin Yan
Summary: This study investigated the role of umbilical cord mesenchymal stem cell-derived exosomes in IBD-related fibrosis and its mechanism, providing new ideas for the prevention and treatment of IBD-related intestinal fibrosis.
STEM CELLS INTERNATIONAL
(2023)
Article
Gastroenterology & Hepatology
Fuji Yang, Yanshuang Wu, Yifei Chen, Jianbo Xi, Ying Chu, Jianhua Jin, Yongmin Yan
Summary: Human umbilical cord mesenchymal stem cell-derived exosomes (MSC-ex) may prevent HFD-induced NAFLD via CAMKK1-mediated lipid homoeostasis regulation.
Article
Cell & Tissue Engineering
Haoxi Ni, Jianbo Xi, Jianjun Tang, Yongmin Yan, Ying Chu, Jing Zhou
Summary: Wound healing in chronic wounds is a complex problem. Traditional methods have poor treatment results and cause psychological stress to patients and economic burden to society. Stem cell-derived extracellular vesicles (SC-EVs) have shown potential in accelerating chronic wound repair due to their ability to inhibit inflammation, induce angiogenesis, promote re-epithelization, and reduce scar formation. This study summarizes the pathological factors hindering wound healing, discusses the mechanisms of how SC-EVs accelerate chronic wound repair, compares the advantages and disadvantages of different SC-EVs for chronic wound treatment, and provides new insights for future SC-EVs research in chronic wound treatment.
STEM CELL REVIEWS AND REPORTS
(2023)
Article
Oncology
Cheng Ji, Jiahui Zhang, Zixuan Zhou, Hui Shi, Wanzhu Liu, Fengtian Sun, Chenxiao Zhang, Leilei Zhang, Zixuan Sun, Hui Qian
Summary: The study proposed the therapeutic use of platelet-rich plasma-stimulated mesenchymal stem cells (PRP-MSCs) for acute kidney injury (AKI), showing significant improvement in kidney damage and reduced levels of blood urea nitrogen and creatinine in both in vivo and in vitro studies. The results suggest that PRP can enhance the therapeutic effect of MSCs through a paracrine pathway, offering a promising treatment option for AKI.
AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH
(2021)
