期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 55, 期 8, 页码 2709-2713出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201509389
关键词
biosensor; paper; point of care diagnostics; pullulan; rolling circle amplification
资金
- Natural Sciences and Engineering Council of Canada
- Pro-Lab Diagnostics
- Canada Foundation for Innovation
- Ontario Ministry of Research and Innovation through an Ontario Research Fund grant
We report on a paper device capable of carrying out target-induced rolling circle amplification (RCA) to produce massive DNA amplicons that can be easily visualized. Interestingly, we observed that RCA was more proficient on paper than in solution, which we attribute to a significantly higher localized concentration of immobilized DNA. Furthermore, we have successfully engineered a fully functional paper device for sensitive DNA or microRNA detection via printing of all RCA-enabling molecules within a polymeric sugar film formed from pullulan, which was integrated with the paper device. This encapsulation not only stabilizes the entrapped reagents at room temperature but also enables colorimetric bioassays with minimal steps.
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