期刊
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
卷 55, 期 42, 页码 13159-13163出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201607483
关键词
activity-based probes; cellular hypoxia; dynamic biology; mass spectrometry; tellurium
资金
- Canadian Cancer Society
- Natural Sciences and Engineering Research Council of Canada
- Fluidigm Canada
Changes in the oxygenation state of microenvironments within solid tumors are associated with the development of aggressive cancer phenotypes. Factors that influence cellular hypoxia have been characterized; however, methods for measuring the dynamics of oxygenation at a cellular level in vivo have been elusive. We report a series of tellurium-containing isotopologous probes for cellular hypoxia compatible with mass cytometry (MC)-technology that allows for highly parametric interrogation of single cells based on atomic mass spectrometry. Sequential labeling with the isotopologous probes (SLIP) in pancreatic tumor xenograft models revealed changes in cellular oxygenation over time which correlated with the distance from vasculature, the proliferation of cell populations, and proximity to necrosis. SLIP allows for capture of spatial and temporal dynamics in vivo using enzyme activated probes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据