Article
Cell Biology
Tobias Maetzig, Anna Lieske, Nicole Doerpmund, Michael Rothe, Marc-Jens Kleppa, Violetta Dziadek, Jacob Jalil Hassan, Julia Dahlke, Dorit Borchert, Axel Schambach
Summary: This study developed a complex fluorescent genetic barcoding system for the tracking and analysis of AML clones. It revealed the competitive dynamics and evolution of AML clones and verified the underlying driver mechanisms behind clonal selection.
Article
Biochemical Research Methods
Qiaodong Wei, Ying Xiong, Yuhang Ma, Deyun Liu, Yunshu Lu, Shenghong Zhang, Xiaolong Wang, Huaxiong Huang, Yingbin Liu, Ming Dao, Xiaobo Gong
Summary: The physical and mechanical properties of red blood cells (RBCs) play an important role in disease diagnosis, but it is challenging to measure these properties accurately due to the unique shape and high heterogeneity of RBCs. In this study, a microfluidic technology combined with machine learning is developed to measure the mechanical properties of individual RBCs. The method can detect subtle changes in RBC components in response to different doses of drugs and explore the correlation between glycosylated hemoglobin and RBC mechanical properties. This approach has the potential to offer novel label-free single-cell-assay-based biophysical markers for RBCs, enhancing the potential for more robust disease diagnosis.
Article
Chemistry, Multidisciplinary
Hossein Tavassoli, Prunella Rorimpandey, Young Chan Kang, Michael Carnell, Chris Brownlee, John E. Pimanda, Peggy P. Y. Chan, Vashe Chandrakanthan
Summary: A label-free method using inertial microfluidics is developed to purify viable cardiomyocytes from mouse neonatal hearts with purities over 90%. The isolated cardiomyocytes retain their identity and function, exhibiting different physico-mechanical properties compared to non-cardiomyocytes. This method could be a valuable tool for advancing the understanding of cardiomyocyte identity and function, with potential benefits for cell therapy and diagnostic applications.
Article
Chemistry, Multidisciplinary
Yao Chen, Chen Ni, Lin Jiang, Zhonghua Ni, Nan Xiang
Summary: An inertial multi-force deformability cytometry (IMFDC) is developed for high-throughput, high-accuracy, and high-applicability tumor cell mechanotyping. The IMFDC is able to evaluate the deformability of different cells and identify cell types. The multiple-parameters-based machine learning model achieves high discrimination accuracy and throughput. The IMFDC has important applications in clinical cancer diagnosis.
Review
Chemistry, Analytical
Zheng Zhou, Yao Chen, Shu Zhu, Linbo Liu, Zhonghua Ni, Nan Xiang
Summary: Inertial microfluidics, proposed in 2007, has been extensively studied and applied in the biomedical field, offering advantages such as high throughput, simplicity, and low cost. This technology is promising for rapid sample processing, cell analysis, and detection, playing a crucial role in cellular sample pretreatment.
Article
Biophysics
Yen-Cheng Liu, Saeid Ansaryan, Xiaokang Li, Eduardo R. Arvelo, Hatice Altug
Summary: This study presents a high-throughput biosensing microarray platform for real-time monitoring of secreted proteins from living single cells. It utilizes an ultrasensitive nanoplasmonic substrate and microwell compartments to analyze secretome at single-cell resolution. The platform also allows optical imaging of cells to track morphology changes. The system demonstrated its potential by detecting interleukin-2 secretion from single lymphoma cells in real-time.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Multidisciplinary
Joshua A. Welsh, Bryce Killingsworth, Julia Kepley, Tim Traynor, Kathy McKinnon, Jason Savage, Deven Appel, Kenneth Aldape, Kevin Camphausen, Jay A. Berzofsky, Alexander R. Ivanov, Ionita H. Ghiran, Jennifer C. Jones
Summary: Evidence is increasing for the clinical utility of extracellular vesicles (EVs) as translational biomarkers, but there is a lack of methods for removing unbound labels. By developing a multi-modal resin method called EV-Clean, improvements have been demonstrated in flow cytometric EV analysis.
Article
Biology
Ziqi Zhang, Kelvin C. M. Lee, Dickson M. D. Siu, Michelle C. K. Lo, Queenie T. K. Lai, Edmund Y. Lam, Kevin K. Tsia
Summary: This article introduces an image-based approach that can quantify multiple biophysical fractal-related properties of single cells at subcellular resolution. This technique has high-throughput single-cell imaging performance and offers sufficient statistical power for cell classification, drug response assays, and cell-cycle progression tracking.
COMMUNICATIONS BIOLOGY
(2023)
Article
Chemistry, Analytical
Gerjen H. Tinnevelt, Kristiaan Wouters, Geert J. Postma, Rita Folcarelli, Jeroen J. Jansen
Summary: White blood cells play a crucial role in protecting the body from diseases, but can also be related to chronic inflammation, auto immune diseases, or leukemia. High-throughput analytical instruments have been developed to measure multiple proteins on millions of single cells to study the identity and function of different white blood cell types. Multivariate statistics are essential for fully extracting the information-rich biochemistry data. The process involves analyzing the study design, formulating a research question, preparing the data, converting single cells into a cellular distribution, and using clustering methods or models for analysis to differentiate cell (sub)types between groups.
ANALYTICA CHIMICA ACTA
(2021)
Review
Chemistry, Analytical
Majood Haddad, Alex N. Frickenstein, Stefan Wilhelm
Summary: Understanding nanoparticle-cell interactions at single-nanoparticle and single-cell resolutions is crucial to improving the design of next-generation nanoparticles for safer, more effective, and more efficient applications in nanomedicine. This review focuses on recent advances in the continuous high-throughput analysis of nanoparticle-cell interactions at the single-cell level, discussing methods such as flow cytometry and inductively coupled plasma mass spectrometry, as well as their intersection in mass cytometry. The review also explores the challenges and opportunities in current single-cell analysis approaches and proposes directions for innovation in high-throughput analysis of nanoparticle-cell interactions.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Biophysics
Minhui Liang, Qiang Tang, Jianwei Zhong, Ye Ai
Summary: Microfluidics provides a powerful platform for biological analysis by precisely manipulating fluids and microparticles. The imaging and impedance cell analyzer (IM2Cell) introduced here combines single cell level impedance analysis and hydrodynamic mechanical phenotyping, demonstrating multi-stress level mechanical phenotyping capabilities. IM2Cell can characterize cell diameter, deformability responses, and electrical properties, providing high-dimensional information about subcellular components. It has been validated for different cell lines and shows potential for deformability studies of PBMC subpopulations.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Multidisciplinary Sciences
Brian D. Reed, Michael J. Meyer, Valentin Abramzon, Omer Ad, Pat Adcock, Faisal R. Ahmad, Gun Alppay, James A. Ball, James Beach, Dominique Belhachemi, Anthony Bellofiore, Michael Bellos, Juan Felipe Beltran, Andrew Betts, Mohammad Wadud Bhuiya, Kristin Blacklock, Robert Boer, David Boisvert, Norman D. Brault, Aaron Buxbaum, Steve Caprio, Changhoon Choi, Thomas D. Christian, Robert Clancy, Joseph Clark, Thomas Connolly, Kathren Fink Croce, Richard Cullen, Mel Davey, Jack Davidson, Mohamed M. Elshenawy, Michael Ferrigno, Daniel Frier, Saketh Gudipati, Stephanie Hamill, Zhaoyu He, Sharath Hosali, Haidong Huang, Le Huang, Ali Kabiri, Gennadiy Kriger, Brittany Lathrop, An Li, Peter Lim, Stephen Liu, Feixiang Luo, Caixia Lv, Xiaoxiao Ma, Evan McCormack, Michele Millham, Roger Nani, Manjula Pandey, John Parillo, Gayatri Patel, Douglas H. Pike, Kyle Preston, Adeline Pichard-Kostuch, Kyle Rearick, Todd Rearick, Marco Ribezzi-Crivellari, Gerard Schmid, Jonathan Schultz, Xinghua Shi, Badri Singh, Nikita Srivastava, Shannon F. Stewman, T. R. Thurston, Philip Trioli, Jennifer Tullman, Xin Wang, Yen-Chih Wang, Eric A. G. Webster, Zhizhuo Zhang, Jorge Zuniga, Smita S. Patel, Andrew D. Griffiths, Antoine M. Van Oijen, Michael McKenna, Matthew D. Dyer, Jonathan M. Rothberg
Summary: This study presents a dynamic approach for single-molecule protein sequencing, where single peptides are probed in real time using a mixture of dye-labeled amino acid recognizers and simultaneously cleaved by aminopeptidases. The peptide sequence is identified by measuring fluorescence intensity, lifetime, and binding kinetics. This method enables discrimination of single amino acid substitutions and posttranslational modifications.
Article
Chemistry, Analytical
Lihui Yang, Xiaobo Liu, Bing Yin, Xunxun Deng, Xiaotong Lin, Jie Song, Shuo Wu
Summary: Real-time monitoring of extracellular pH at the single-cell level is critical for understanding disease mechanisms and investigating drug effects, especially in cancer cells. Developing a reliable method with good selectivity, reproducibility, and stability for measuring pHe heterogeneity in cancer cells is necessary. The high-throughput, real-time measuring technique based on polyaniline micro-electrode arrays in this study showed high sensitivity and reliability for monitoring single-cell pHe, revealing significant cellular heterogeneity in pHe changes of different cancer cells.
ANALYTICAL CHEMISTRY
(2021)
Article
Microbiology
Clarisse Marotz, James T. Morton, Perris Navarro, Joanna Coker, Pedro Belda-Ferre, Rob Knight, Karsten Zengler
Summary: This study successfully quantified the number of live microbial cells in the oral cavity using flow cytometry and relic DNA removal method in conjunction with metagenomic sequencing. The results showed an inverse correlation between live microbial load and salivary flow rate, with significant fluctuations throughout the day. These findings provide new insights for longitudinal study designs and microbial composition analysis.
Article
Biochemical Research Methods
Ning Yang, Taiwei Li, Sizhe Dong, Suliang Zhang, Yanwei Jia, Hanping Mao, Zhen Zhang, Fu Zhang, Xiaoqing Pan, Xiaodong Zhang, Zining Dong
Summary: This article introduces an innovative airflow-based optical detection method for directly identifying aerosol pathogens, and a simplified detection platform that reduces sample preparation and detection steps. The method achieves high particle counting accuracy and real-time identification accuracy. Potential applications of this method in disease control, environmental control, and aerosol pathogen detection are foreseen.
Article
Biochemical Research Methods
Marta Urbanska, Hector E. Munoz, Josephine Shaw Bagnall, Oliver Otto, Scott R. Manalis, Dino Di Carlo, Jochen Guck
Article
Biology
Laura Sachs, Jan Wesche, Lea Lenkeit, Andreas Greinacher, Markus Bender, Oliver Otto, Raghavendra Palankar
Summary: This study examines the impact of different ex vivo anticoagulants on the biomechanical and functional properties of single platelets. The results show that the choice of anticoagulant can greatly influence the mechanophenotyping outcomes. It is unclear whether ex vivo anticoagulants used during blood collection affect the mechanophenotype of blood components.
COMMUNICATIONS BIOLOGY
(2022)
Article
Physiology
Ricardo H. Pires, Tung H. Dau, Emmanuel Manu, Nithya Shree, Oliver Otto
Summary: This study investigates the mechanodynamic changes that occur during the differentiation of cardiac progenitor cells into cardiomyocytes. The researchers utilized atomic force microscopy and real-time deformability cytometry to describe these changes and link them to protein expression data. They found that increased levels of cardiac-specific markers and changes in cytoskeletal morphology and contractility parameters correlated with the extent of cell differentiation.
PHYSIOLOGICAL REPORTS
(2022)
Article
Biochemical Research Methods
Bob Fregin, Doreen Biedenweg, Oliver Otto
Summary: The capability to parameterize shapes is important in biomechanics for identifying cells, tracking their motion, and quantifying deformation. This study compared nine different shape descriptors to characterize the deformation of suspended cells. The researchers found that high-throughput viscoelastic measurements of cells in suspension can be performed on an ensemble scale, and different shape descriptors have varying effects on cell deformation evaluation.
Article
Multidisciplinary Sciences
Juliane Baumann, Laura Sachs, Otto Oliver, Ingmar Schoen, Peter Nestler, Carlo Zaninetti, Martin Kenny, Ruth Kranz, Hendrik von Eysmondt, Johanna Rodriguez, Tilman E. Schaeffer, Zoltan Nagy, Andreas Greinacher, Raghavendra Palankar, Markus Bender
Summary: Patients with MYH9-related disease have a tendency for increased bleeding due to reduced platelet forces caused by mutations. Activation of mutant platelets is comparable to controls, but phosphorylation of myosin light chain is reduced. Treatment with tranexamic acid can improve hemostatic function.
Correction
Biology
Laura Sachs, Jan Wesche, Lea Lenkeit, Andreas Greinacher, Markus Bender, Oliver Otto, Raghavendra Palankar
COMMUNICATIONS BIOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Andreas Kuemmel, Stefan Gross, Rico Feldtmann, Bishwas Chamling, Anne Strohbach, Kristin Lehnert, Martin Bahls, Lisa Loerzer, Katharina Moormann, Jeannine Witte, Alexander Riad, Marcus Doerr, Jens Fielitz, Stephan B. Felix
Summary: This study found that the levels of HMGB1 were associated with increased mortality in patients with dilated cardiomyopathy. Multivariable Cox regression analysis confirmed HMGB1 as a risk factor for mortality in DCM patients, independent of NT-proBNP, age, and gender.
AMERICAN JOURNAL OF CARDIOLOGY
(2022)
Article
Cell Biology
Venkata A. S. Dabbiru, Emmanuel Manu, Doreen Biedenweg, Peter Nestler, Ricardo H. Pires, Oliver Otto
Summary: Alterations in cytoskeletal organization can lead to the escape of adherent cells from cell-cell and cell-matrix interactions into suspension. This study investigates the relationship between cytoskeletal dynamics, cell mechanical properties, and changes in cellular shape, volume, and mechanical properties under different cellular states. The findings reveal an interplay between molecular and mechanical alterations during cell detachment from a surface, predominantly influenced by cell morphology.
Article
Biochemical Research Methods
Yesaswini Komaragiri, Muzaffar H. Panhwar, Bob Fregin, Gayatri Jagirdar, Carmen Wolke, Stefanie Spiegler, Oliver Otto
Summary: This study introduces a new approach to determine the mechanical properties of individual mitochondria in a high-throughput manner and investigates the mechanical alterations of mitochondria under oxidative stress conditions.
Letter
Cardiac & Cardiovascular Systems
Camila Campos Santana, Anke Hannemann, Till Ittermann, Stefan Gross, Guillermo Pierdant, Jan Schielke, Martin Bahls, Henry Voelzke, Nele Friedrich, Stephan Burkhard Felix, Elisabeth Steinhagen-Thiessen, Marcus Doerr, Marcello Ricardo Paulista Markus
EUROPEAN JOURNAL OF PREVENTIVE CARDIOLOGY
(2023)
Article
Medicine, General & Internal
Anke Steinmetz, Susanne Bahlmann, Corinna Bergelt, Barbara M. M. Broeker, Ralf Ewert, Stephan B. B. Felix, Agnes Floeel, Robert Fleischmann, Wolfgang Hoffmann, Silva Holtfreter, Matthias Nauck, Katja Riemann, Christian Scheer, Dana Stahl, Antje Vogelgesang, Uwe Voelker, Ulrich Wiesmann, Johanna Klinger-Koenig, Rene Walk, Hans J. J. Grabe, Stefan Gross, Kristin Lehnert, Jens Fielitz, Marcus Doerr
Summary: COVID-19 is associated with long-term symptoms and disability known as post-COVID syndrome (PCS). The pathogenesis of PCS is not yet understood and treatment is limited to symptomatic relief. The Greifswald Post COVID Rehabilitation Study (PoCoRe) aims to follow and comprehensively analyze the symptoms, signs, and biomarkers of PCS outpatients in the long term.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Medicine, General & Internal
Anke Steinmetz, Stefan Gross, Kristin Lehnert, Petra Luecker, Nele Friedrich, Matthias Nauck, Susanne Bahlmann, Jens Fielitz, Marcus Doerr
Summary: Post-COVID-19 syndrome, also known as the pandemic after the pandemic, has affected over 65 million people worldwide. The wide range of symptoms makes diagnosis complex and treatment difficult. A comprehensive diagnostic assessment was conducted on 184 non-hospitalized patients in a post-COVID rehabilitation clinic, revealing that fatigue, decreased physical capacity, tiredness, poor concentration, sleeping problems, and shortness of breath were the most common symptoms. Abnormalities were found in various assessments and tests, indicating a clear association between objective measures and pronounced symptoms.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Medicine, General & Internal
Katharina Knoll, Patrick Fuchs, Isabel Weidmann, Fatih Altunkas, Stephanie Voss, Carsten Lennerz, Christof Kolb, Thorsten Kessler, Heribert Schunkert, Wibke Reinhard, Stefan Gross, Teresa Trenkwalder
Summary: The incidence of ventricular arrhythmias in wtATTR-CM is 44% and is associated with the severity of left ventricular disease. Further studies are needed to evaluate the role of ventricular arrhythmias in predicting sudden cardiac death and the benefit of ICD therapy in wtATTR-CM.
JOURNAL OF CLINICAL MEDICINE
(2023)
