期刊
ONCOLOGY LETTERS
卷 17, 期 4, 页码 3842-3850出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/ol.2019.10052
关键词
breast cancer; hTERT; luteolin; cell cycle; telomerase
类别
资金
- Zhejiang Provincial Science and Technology Projects of Traditional Chinese Medicine [2017ZB089]
- National Natural Science Foundation of China [81772537, 81374014]
- Zhejiang Provincial Medical and Healthy Science and Technology Projects [2018ZD047, 2016ZDB013]
- Zhejiang Provincial Science and Technology Projects [LGF18H160041, 2017C33212, 2017C33213, 2015C33264]
Luteolin is a flavonoid, which has been extensively investigated due to its antitumor effects; however, the underlying mechanisms of its action remain largely unknown. The present study aimed to investigate the role of luteolin in breast cancer (BC), and explored how luteolin suppresses the growth and induces the apoptosis of BC cells. The MTS assay was used to determine the anticancer activity of luteolin. Colony formation and Transwell assays were performed to evaluate the effects of luteolin on cell growth and invasion. Cell cycle progression and apoptosis were analyzed by flow cytometry. In addition, western blotting was performed to analyze cellular apoptosis and signaling pathways elicited by luteolin. The present study revealed that the proliferation of the BC cell line MDA-MB-231 was effectively suppressed by luteolin in a dose-dependent manner. Additionally, luteolin was revealed to increase apoptotic rates in BC cells. Dose-dependent cell cycle arrest in S phase was observed following treatment with luteolin in MDA-MB-231 cells. Mechanistically, luteolin reduced telomerase levels in a dose-dependent manner. Additionally, luteolin inhibited phosphorylation of the nuclear factor-kappa B inhibitor alpha and its target gene c-Myc, to suppress human telomerase reverse transcriptase (hTERT) expression, which encodes the catalytic subunit of telomerase. Collectively, the results of the present study indicated that luteolin may inhibit BC cell growth by targeting hTERT, suggesting that the mechanism of hTERT regulation by luteolin may justify further study regarding its potential as a therapeutic target for BC treatment.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据