Review
Plant Sciences
Shumei Fang, Xue Hou, Xilong Liang
Summary: SUMOylation is a crucial posttranslational modification in eukaryotes, involved in metabolic regulation under various stresses. SIZ1, a SUMO E3 ligase, plays a vital role in substrate-specific recognition and facilitates the binding of SUMO proteins to target proteins. This review provides insights into the characteristics of SUMOylation and the role of SIZ1 in plant adaptation to abiotic stresses, offering valuable references for crop improvement and future research on plant responses to environmental stress.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2022)
Review
Biochemistry & Molecular Biology
Samuel Smalley, Hanjo Hellmann
Summary: This review provides an overview of how ubiquitin and similar proteins modify target proteins in plants to rapidly change their stability and activity, with a focus on their contribution to stress tolerance. The study also explores the potential use of these pathways for generating more robust crop plants to cope with stress situations.
Article
Cell Biology
Sujuan Gao, Xueqin Zeng, Jianhao Wang, Yingchao Xu, Chunwei Yu, Yishui Huang, Feng Wang, Keqiang Wu, Songguang Yang
Summary: The study reveals that the Arabidopsis SUMO E3 ligase SIZ1 interacts with HDA6 and negatively regulates the function of HDA6 by affecting the acetylation level of histone H3 and the binding of HDA6 to its target genes.
Article
Biochemistry & Molecular Biology
Jiawei Xiong, Fabin Yang, Fan Wei, Feng Yang, Honghui Lin, Dawei Zhang
Summary: HLS1 plays a key role in the development of the apical hook in plants, and SIZ1 regulates the rapid opening of the apical hook by mediating HLS1 SUMOylation. The opening of the apical hook is associated with a decrease in SIZ1 transcript levels, leading to reduced HLS1 SUMOylation. In addition, HY5 promotes the rapid opening of the apical hook by inhibiting SIZ1 transcription.
Article
Multidisciplinary Sciences
Terry R. Suk, Marilyn T. Tirard, Zoe A. Fisk, Miso Mitkovski, Haley M. Geertsma, Jean-Louis A. Parmasad, Meghan M. Heer, Steve M. Callaghan, Fritz Benseler, Nils Brose, Marilyn Tirard, Maxime W. C. Rousseaux
Summary: SUMOylation is a protein modification widely present in evolutionarily conserved eukaryotes and has broad biological significance. To differentiate between SUMO paralogs and uncover their specific functions in vivo, His6-HA-Sumo2 and HA-Sumo2 knockin mouse lines were generated, expanding upon the existing His6-HA-Sumo1 mouse line. By utilizing the specificity of the HA epitope, regional differences in Sumo1 and Sumo2 expression were observed through whole-brain imaging. Immunoprecipitation coupled with mass spectrometry identified shared and specific neuronal targets of Sumo1 and Sumo2, and proximity ligation assays provided insight into the subcellular distribution of neuronal Sumo2-conjugates. These mouse models and associated datasets provide a powerful framework to determine the native SUMO code in cells of the central nervous system.
Article
Biochemistry & Molecular Biology
Yubin Xie, Huiqin Li, Xiaotong Luo, Hongyu Li, Qiuyuan Gao, Luowanyue Zhang, Yuyan Teng, Qi Zhao, Zhixiang Zuo, Jian Ren
Summary: The visualization of biological sequences is crucial in the field of molecular and cellular biology. IBS 2.0 is a professional tool that allows concise and clear representation of protein and nucleotide sequences. It provides abundant graphical elements and database visualization module for batch visualization of biological sequences.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Plant Sciences
Shan Liu, Camille J. G. Lenoir, Tiago M. M. M. Amaro, Patricia A. Rodriguez, Edgar Huitema, Jorunn I. B. Bos
Summary: Pathogens and pests utilize different strategies to enhance host susceptibility through manipulation of the E3 SUMO ligase SIZ1, with oomycete and aphid effectors displaying distinct activities towards SIZ1.
Article
Biochemistry & Molecular Biology
Wenjia Li, Qingfang Han, Yuanxin Zhu, Yingshi Zhou, Jingyuan Zhang, Weijun Wu, Yu Li, Long Liu, Yuntan Qiu, Kaishun Hu, Dong Yin
Summary: It is discovered that RNF146, a RING-type E3 ubiquitin ligase, can be modified by SUMO3 at K19, K61, K174, and K175. SUMOylation promotes the association of RNF146 with Axin, leading to the acceleration of Axin degradation and enhancement of β-catenin signaling pathway, thus contributing to the progression of cancer. Inhibiting RNF146 SUMOylation suppresses the progression of HCC.
Article
Virology
Behdokht Jan Fada, Udayan Guha, Yi Zheng, Eleazar Reward, Elie Kaadi, Ayette Dourra, Haidong Gu
Summary: It was found that ICP0 E3 enzyme continuously degrades PML I through a specific mechanism to prevent the reformation of ND10 complex.
Article
Biochemistry & Molecular Biology
Gurusamy Hariharasudhan, Seo-Yeon Jeong, Min-Ji Kim, Sung Mi Jung, Gwanwoo Seo, Ju-Ran Moon, Sumi Lee, In-Youb Chang, Younghoon Kee, Ho Jin You, Jung-Hee Lee
Summary: SUMOylation of RAD51 is crucial for its recruitment to chromatin and HR repair. TOPORS-mediated RAD51 SUMOylation plays a key role in promoting HR repair and genomic maintenance.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Rahul M. Nair, Ayshwarya Seenivasan, Bing Liu, Dan Chen, Edward D. Lowe, Sonja Lorenz
Summary: This study demonstrates the use of an activity-based probe, ubiquitin-propargylamine, for reconstituting and analyzing the interactions between ubiquitin and certain HECT ligases. The crystal structure reveals a catalytically critical conformation of the C-terminal tail of the ligase for ubiquitin transfer. Additionally, the probe shows selectivity among HECT domains, suggesting potential for developing specific inhibitors and reporters of HECT ligase activities.
ACS CHEMICAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Xiaoxia Shi, Yixin Du, Shujing Li, Huijian Wu
Summary: SUMOylation, a reversible post-translational modification process, plays crucial roles in cell physiology, including cell cycle regulation, DNA damage repair, and protein trafficking and turnover. Dysregulated SUMO E3 ligases may be associated with the occurrence and development of cancers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Daniel Salas-Lloret, Roman Gonzalez-Prieto
Summary: Ubiquitination and SUMOylation are dynamic post-translational modifications that regulate cellular processes. The understanding of these modifications has been increased by the development of mass spectrometry-based approaches. This translated article provides a concise overview of the basic mechanisms of ubiquitination and SUMOylation, as well as recent MS-based approaches for specific target identification.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Xinyang Liao, Juan Sun, Quanquan Li, Wenyan Ding, Binbin Zhao, Baobao Wang, Shaoqun Zhou, Haiyang Wang
Summary: In this study, it was found that ZmSIZ1a and ZmSIZ1b play a crucial role in resisting FER, and they have a redundant but indispensable function in the resistance process. Integrating transcriptome and metabolome data, it was discovered that the synthesis pathway of flavonoids was induced by F. verticillioides infection and significantly reduced in the Zmsiz1a/1b double mutant. These findings provide potential new gene resources for molecular breeding of FER-resistant maize cultivars.
MOLECULAR PLANT PATHOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Bin Lv, Yida Pan, Daisen Hou, Ping Chen, Jun Zhang, Yiwei Chu, Mingqi Li, Yan Zeng, Dongqin Yang, Jie Liu
Summary: This study found that ring finger protein 4 (RNF4) is essential for the growth of hepatocellular carcinoma (HCC). Silencing RNF4 suppressed HCC cell growth, induced apoptosis, increased DNA damage, and enhanced sensitivity to DNA damage drugs and radiation. These findings suggest that RNF4 may serve as a novel target for suppressing HCC growth and improving the efficacy of DNA damage therapeutic agents.