期刊
VACCINE
卷 37, 期 12, 页码 1554-1564出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.vaccine.2019.02.005
关键词
Mucosal immune response; Malate dehydrogenase; Brucella abortus; Chitosan nanoparticles; Nasal immunization
资金
- Korea Health Industry Development Institute (KHIDI) [HI16C2130]
- Brain Korea (BK) 21 PLUS program for Creative Veterinary Science Research
- Research Institute of Veterinary Science at Seoul National University in the Republic of Korea
The aim of this study was to investigate the induction of mucosal immune responses by an important Brucella abortus antigen, malate dehydrogenase (Mdh), loaded in mucoadhesive chitosan nanoparticles (CNs) and immunized intranasally in a BALB/c mouse model. The production of cytokines was investigated in human leukemic monocyte cells (THP-1 cells) after stimulation with the nanoparticles. Mdh-loaded CNs (CNs-Mdh) induced higher interleukin (IL)-6 production than unloaded antigens and TF loaded CNs (CNs-TF). Using ELISpot to quantify cytokines and antibody-secreting cells in the intranasally immunized mice, IL-4 and IgG-secreting cells were found to be significantly increased at 4 weeks and 6 weeks post-immunization in the CNs-Mdh immunized group, respectively. Increases in Mdh-specific IgG, IgG1, and IgG2a antibodies were confirmed at 6 weeks after immunization, indicating a predominant IgG1 response. Analysis of the mucosal immune response in the intranasally immunized mice revealed, Mdh-specific IgA and total IgA in the nasal washes, genital secretions, fecal extracts and sera that were remarkably increased in the CNs-Mdh-immunized group compared to the CNs-TF-immunized group except total IgA of nasal wash. Therefore, the results indicated that the intranasal immunization of CNs-loaded B. abortus Mdh antigen effectively induced antigen-specific mucosal immune responses through the elicitation of Th2-related immune responses. (C) 2019 Published by Elsevier Ltd.
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