期刊
TOXICOLOGY IN VITRO
卷 54, 期 -, 页码 105-113出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.tiv.2018.08.014
关键词
3D human placenta-on-a-chip; Nanoparticle exposure; Organ-on-a -chip
类别
资金
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDA16020900, XDPB0305]
- International Science & Technology Cooperation Program of China [2015DFA00740]
- Key Program of the Chinese Academy of Sciences [KFZD-SW-213]
- National Key R&D Program of China [2017YFB0405400]
- National Nature Science Foundation of China [91543121, 81573394, 31671038, 81603075, 21607151]
- Innovation Program of Science and Research from the DICP, CAS [DICP TMSR201601]
With the commercialization of nanomaterials, environmental exposure to nanoparticles (NPs) has raised great concerns due to the long-term effects to human body, particularly to pregnant women. Previous studies found that NPs had an adverse impact on placenta in mice, but care must be taken when extrapolating the results to human pregnancy in consideration of the great difference between species. Here, we proposed a micro engineered 3D placental barrier-on-a-chip microdevice and further explored complicated placental responses to NPs exposure in vitro. The microdevice recreated near-physiological 3D microenvironment and dynamic conditions in fetal maternal circulation combined with the extracellular matrix and flow. With the exposure to titanium dioxide nanoparticles (TiO2-NPs), a common nanomaterial, a series of placental responses were investigated, including oxidative stress, cell apoptosis, barrier permeability, and maternal immune cell behavior. By contrast to oxidative stress and cell apoptosis, placental barrier integrity and maternal immune cells were greatly influenced even with low concentrated NPs, suggesting the potential damages triggered by NPs in our daily life. Collectively, this in vitro experimental model of human placenta provides a simple platform to study environmental exposure to NPs, and might be potential for a wide range of applications in biological study, disease treatment and drug assessment.
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