Article
Medicine, General & Internal
Alessandra Colombini, Francesca Libonati, Silvia Lopa, Enrico Ragni, Paola De Luca, Luigi Zagra, Federico Sinigaglia, Matteo Moretti, Laura de Girolamo
Summary: This study predicted the immunomodulatory potential of miRNAs secreted by cartilage cells, adipose tissue-derived stem cells, and bone marrow-derived stem cells through bioinformatics analysis, supporting the rationale behind the use of cell-based therapy for osteoarthritis management.
FRONTIERS IN MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Anna Trzyna, Agnieszka Banas-Zabczyk
Summary: The secretome of ASCs, including exosomes, contains a variety of cytokines and non-coding RNAs that have therapeutic potential in a wide range of diseases. Utilizing ASC secretome as a cell-free therapy shows promise in overcoming limitations of cell-based therapies and improving treatment efficacy.
Review
Cell Biology
Leandra S. Baptista, Karina R. Silva, Lara Jobeili, Lucile Guillot, Dominique Sigaudo-Roussel
Summary: The dysfunctions of white adipose tissue in immune and endocrine systems are key characteristic of metabolic disorders like obesity and type 2 diabetes. White adipose tissue in humans is distributed in distinct depots under the skin (hypodermis) and internally (visceral). Depot-specific adult stem cells (ASCs) have the ability to regulate adipogenesis and immunomodulation, thus accounting for the properties of visceral and subcutaneous adipose tissues. Recent studies have discovered different subpopulations of ASCs in subcutaneous adipose tissue, demonstrating heterogeneity within the white adipose tissue. Understanding the biology of these ASC subpopulations and utilizing adipose tissue 3D models can contribute to elucidating the role of ASCs in obesity development.
Article
Engineering, Biomedical
Feng Zhao, Tianli Yang, Liuhua Zhou, Jun Zhao, Jingyu Liu, Wenwen Ping, Changcheng Zhou, Zhiqiang Qin, Ruipeng Jia
Summary: Tissue engineering approaches offer promising alternative strategies for reconstructing bladder tissue. In this study, an artificial nanoscaffold system was developed to promote tissue regeneration and restore bladder function. The system demonstrated potent proangiogenic ability and induced M2 macrophage polarization. The study shows the safety and efficacy of the artificial nanoscaffold, which has the potential for clinical application.
ACTA BIOMATERIALIA
(2023)
Article
Biochemistry & Molecular Biology
Ha Thi-Ngan Le, Ngoc Bich Vu, Phuc Dang-Ngoc Nguyen, Thuy Thi-Thanh Dao, Xuan Hoang-Viet To, Phuc Van Pham
Summary: This study proposed a new method to produce cartilage tissues in vitro using adipose-derived stem cells in spheroids on porous scaffolds, showing promising results in rat models by demonstrating successful chondrogenic differentiation and maturation into cartilage tissues.
FRONTIERS IN BIOSCIENCE-LANDMARK
(2021)
Article
Cell Biology
Liang Lu, Xifu Shang, Bin Liu, Weijian Chen, Yu Zhang, Shuyun Liu, Xiang Sui, Aiyuan Wang, Quanyi Guo
Summary: The study demonstrated that the cartilage complex constructed by ADSCs and ACECM through tissue engineering successfully repaired cartilage defects, indicating the potential of ADSCs for chondrogenic differentiation.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Biochemical Research Methods
Zhuhao Wu, Bin Chen, Yue Wu, Yu Xia, Hui Chen, Zhiyi Gong, Hang Hu, Zhao Ding, Shishang Guo
Summary: A scaffold-free acoustofluidic device was developed to fabricate heterotypic cell spheroids with complexity over cell architectures and components, demonstrating potential in various areas such as tumor research, tissue engineering, developmental biology, and drug discovery.
Article
Pharmacology & Pharmacy
Gwenda F. Vasse, Lisette Van Os, Marina De Jager, Marnix R. Jonker, Theo Borghuis, L. Tim Van den Toorn, Pytrick Jellema, Eric S. White, Patrick Van Rijn, Martin C. Harmsen, Irene H. Heijink, Barbro N. Melgert, Janette K. Burgess
Summary: Idiopathic pulmonary fibrosis (IPF) is characterized by excessive deposition of extracellular matrix (ECM) in the lungs, with differences in collagen structure observed. This study found that adipose tissue-derived stromal cell conditioned medium (ASC-CM) treatment can reduce ECM deposition in lung tissue, independent of collagen integrity. This suggests that ASC-CM may be a potential treatment for IPF by inhibiting profibrotic behavior of fibroblasts induced by fibrotic ECM.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Cell Biology
Isis Cortes, Gutemberg Alves, Cesar Claudio-Da-Silva, Leandra Santos Baptista
Summary: This study found that different subpopulations of adipose stem/stromal cells (ASCs) exhibit distinct physiological properties in a 3D spheroid culture. ASC spheroids from superficial subcutaneous adipose tissue (sSAT) showed the highest response to adipogenic induction, while spheroids from deep subcutaneous adipose tissue (dSAT) showed the greatest lipolytic capacity. Spheroids from superficial retinacula cutis (sRC) exhibited a greater secretory capacity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Xifeng Liu, Linli Li, Bipin Gaihre, Sungjo Park, Yong Li, Andre Terzic, Benjamin D. Elder, Lichun Lu
Summary: Functional spheroids engineered with two-dimensional heteronano-layers consisting of black phosphorus and graphene oxide show promising potential for bone tissue engineering. These spheroids successfully support osteogenesis of mesenchymal stem cells, neovascularization, and bone regeneration in vivo, as well as achieve outstanding in vivo osteogenic induction and bone regrowth without prior in vitro culture of osteogenic factor. The shortened overall culture time could be advantageous for clinical translation.
Article
Cell Biology
Jun Ochiai, Larakaye Villanueva, Hope Niihara, Yutaka Niihara, Joan Oliva
Summary: This study developed a method to engineer multilayer adipose stem cell cell sheets (ASCCSs) using chemically defined/serum-free culture media, which can differentiate into osteoblasts and chondrocytes. The number of cells per cell sheet was estimated using cell sheet transmittance, providing important non-invasive information.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Cell & Tissue Engineering
Phuong T. M. Dam, Van T. Hoang, Hue Thi Hong Bui, Le Minh Hang, Duc M. Hoang, Hoang Phuong Nguyen, Ha Thi Lien, Huong Thi Thanh Tran, Xuan-Hung Nguyen, Liem Nguyen Thanh
Summary: An increased expression of negative markers, decreased growth ability, chromosomal abnormalities, and senescent feature were observed in ADMSCs expanded in PS media compared to those cultured in SM media. This study suggests that SM media is more suitable for the expansion of therapeutic ADMSCs, highlighting the importance of quality control for therapeutic MSC products, especially at advanced passages and with older donors.
STEM CELL REVIEWS AND REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Oyku Sarigil, Muge Anil-Inevi, Burcu Firatligil-Yildirir, Yagmur Ceren Unal, Ozden Yalcin-Ozuysal, Gulistan Mese, H. Cumhur Tekin, Engin Ozcivici
Summary: The study used magnetic levitation to create 3D scaffold-free adipocyte structures for cell culture and tissue repair. Results showed potential for drug testing and cell therapy applications in soft tissue damage.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Review
Biochemistry & Molecular Biology
Georgy Sagaradze, Anna Monakova, Anastasia Efimenko
Summary: Adult stem cells are regulated by their specific microenvironments called stem cell niches, and dysfunction of the niche components can lead to chronic or acute disorders. To address this, regenerative medicine treatments such as gene and cell therapy are being investigated, with a focus on mesenchymal stromal cells (MSCs) and their secretomes. However, the development of MSC secretome-based products is not fully regulated, which has complicated their clinical translation and resulted in failed trials. This review discusses the development of potency assays for MSC secretome-based products, specifically their effects on stem cell niches and spermatogonial stem cell niche.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Marcelo Coutinho de Miranda, Andrea da Fonseca Ferreira, Mariane Izabella Abreu de Melo, Marianna Kunrath-Lima, Alfredo Miranda de Goes, Michele Angela Rodrigues, Dawidson Assis Gomes, Jerusa Araujo Quintao Arantes Faria
Summary: Mesenchymal stem/stromal cells are recruited by cancer cells to promote tumor events and therapeutic resistance, with their conditioned medium showing the ability to induce increased proliferation and a more aggressive phenotype in breast cancer cells. Understanding the regulatory mechanisms of ASCs in the tumor microenvironment may provide potential avenues for further treatment.
Article
Dentistry, Oral Surgery & Medicine
Fabiola Ormiga, Caroline Chavadian Fonseca Amaral, Felipe Martins Vieira, Patricia de Andrade Risso, Adriana Barbosa da Silva, Marcelo Almeida, Rodrigo Sena, Eduardo Jorge Feres-Filho, Jose Mauro Granjeiro, Jose Antonio da Cunha Ponciano Gomes
Summary: The aim of this study was to measure the ionic quantification in periradicular medium after diffusion tests of the solution used inside root canals. The results showed that dilution of the NiTi-containing dissolution product resulted in significantly lower F-, Ni, and Ti quantities. This suggests that irrigation during the electrochemical dissolution process can reduce ion concentrations.
BRAZILIAN ORAL RESEARCH
(2022)
Article
Materials Science, Biomaterials
Maria de Fatima Santos de Souza, Luiz Augusto Sousa de Oliveira, Fabiana Avila Carneiro, Leandra Santos Baptista, Raquel Moraes Soares
Summary: Superparamagnetic iron oxide nanoparticles show potential applications in therapeutic treatment, with in vitro and in vivo studies revealing biodistribution and toxicity profiles. High doses of SPIONs lead to increased magnetism in the lungs of mice, while causing inflammation and tissue deposition in the liver and kidneys. Further research is needed to ensure safe therapeutic use of these nanoparticles.
Article
Multidisciplinary Sciences
Gabriela S. Kronemberger, Guilherme A. S. C. Miranda, Taisnara I. G. Silva, Rosangela M. Goncalves, Jose M. Granjeiro, Leandra S. Baptista
Summary: This article demonstrates the large-scale production of ASC spheroids using a non-adhesive micromolded hydrogel technique and the use of these spheroids as building blocks for 3D bioprinting approaches.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Chemistry, Multidisciplinary
Thais S. M. Lima, Wanderson Souza, Luths R. O. Geaquinto, Priscila L. Sanches, Ewa L. Stepien, Joao Meneses, Eli Fernandez-de Gortari, Nicole Meisner-Kober, Martin Himly, Jose M. Granjeiro, Ana R. Ribeiro
Summary: The increasing use of nanomaterials has raised concerns about nanosafety and their potential toxic effects on human health. Studies have shown that nanomaterials can alter cell communication by reshaping and altering the secretion of extracellular vesicles (EVs), leading to dysfunction in recipient cells. However, our understanding of how the physicochemical characteristics of nanomaterials affect EV content and their physiological functions is limited. This review explores the relevance of EVs in the field of nanotoxicology and assesses the modulation of EVs by nanomaterial exposure and the regulation of signaling pathways and physiological responses.
Article
Biochemistry & Molecular Biology
Suelen Cristina Sartoretto, Natalia de Freitas Gens, Rodrigo Figueiredo de Brito Resende, Adriana Terezinha Neves Novellino Alves, Rafael Cury Cecato, Marcelo Jose Uzeda, Jose Mauro Granjeiro, Monica Diuana Calasans-Maia, Jose Albuquerque Calasans-Maia
Summary: This study evaluated the biodegradation and inflammatory response of four different experimental versions of PTMC + PLGA membranes. The results showed that these membranes had different reactions and absorption degrees in the tissue. The membranes were considered biocompatible, but some versions had higher absorption performance.
Article
Chemistry, Medicinal
Cecile Hilpert, Louis Beranger, Paulo C. T. Souza, Petteri A. Vainikka, Vincent Nieto, Siewert J. Marrink, Luca Monticelli, Guillaume Launay
Summary: The MArtini Database (MAD) is a web server that facilitates the sharing of structures and topologies of molecules parametrized with the Martini coarse-grained (CG) force field. It offers tools to convert atomistic structures into CG structures, prepare complex systems for MD simulations, and generate input files for Martini 3. MAD is dedicated to providing CG models for various molecules, transforming protein structures, assembling biomolecules, and delivering files necessary for GROMACS MD simulations.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2023)
Article
Chemistry, Multidisciplinary
Gabriela de Souza Castro, Wanderson de Souza, Thais Suelen Mello Lima, Danielle Cabral Bonfim, Jacques Werckmann, Braulio Soares Archanjo, Jose Mauro Granjeiro, Ana Rosa Ribeiro, Sara Gemini-Piperni
Summary: This study compares the effects of different concentrations of TiO2 NPs on the differentiation and mineralized matrix production of human osteoblasts in a monolayer or 3D model. The internalization of nanoparticles and their impact on cell morphology are evaluated. The results demonstrate a dose-dependent increase in mineralized matrix in both the monolayer and 3D models, with higher levels observed in the 3D model after high exposure to TiO2 NPs, suggesting its potential as a model for testing the effect on bone osteointegration.
Article
Endocrinology & Metabolism
Joana R. Dantas, Debora Batista Araujo, Karina Ribeiro Silva, Debora Lopes Souto, Maria de Fatima Carvalho Pereira, Luiz Ronir Raggio, Cesar Claudio-da Silva, Carlos Eduardo Couri, Angelo Maiolino, Carmen Lucia Kuniyoshi Rebellato, Debora Regina Daga, Alexandra Cristina Senegaglia, Paulo Roberto Slud Brofman, Leandra S. Baptista, Jose Egidio Paulo de Oliveira, Lenita Zajdenverg, Melanie Rodacki
Summary: This study aimed to evaluate the safety and therapeutic effect of allogenic adipose tissue-derived stromal/stem cells transplantation with cholecalciferol in patients with recent-onset type 1 diabetes. The results showed that this treatment can reduce insulin requirements, improve glycemic control, and transiently improve pancreatic function in recent onset T1D patients, but the potential benefits were not sustained. Adverse events were minimal and the treatment was safe.
HORMONE AND METABOLIC RESEARCH
(2023)
Review
Cell Biology
Leandra S. Baptista, Karina R. Silva, Lara Jobeili, Lucile Guillot, Dominique Sigaudo-Roussel
Summary: The dysfunctions of white adipose tissue in immune and endocrine systems are key characteristic of metabolic disorders like obesity and type 2 diabetes. White adipose tissue in humans is distributed in distinct depots under the skin (hypodermis) and internally (visceral). Depot-specific adult stem cells (ASCs) have the ability to regulate adipogenesis and immunomodulation, thus accounting for the properties of visceral and subcutaneous adipose tissues. Recent studies have discovered different subpopulations of ASCs in subcutaneous adipose tissue, demonstrating heterogeneity within the white adipose tissue. Understanding the biology of these ASC subpopulations and utilizing adipose tissue 3D models can contribute to elucidating the role of ASCs in obesity development.
Article
Chemistry, Analytical
Elisa Rosa dos Santos, Evaldo Cesar Cavalcante Rodrigues, Jose Mauro Granjeiro
Summary: The biotechnology policy encourages the establishment of institution networks for bioassays and preclinical studies to promote good laboratory practice (GLP) principles. Trained professionals are essential to understand GLP, develop procedures, and implement quality management requirements. The use of multicriteria decision aid (MCDA) can assist in managing and implementing the quality system. This research demonstrates the perception of GLP difficulty levels and identifies efforts to improve understanding through MCDA analysis.
ACCREDITATION AND QUALITY ASSURANCE
(2023)
Article
Chemistry, Medicinal
Gilberto P. Pereira, Brian Jimenez-Garcia, Riccardo Pellarin, Guillaume Launay, Sangwook Wu, Juliette Martin, Paulo C. T. Souza
Summary: Proteolysis targeting chimeras (PROTACs) are emerging as a promising therapeutic approach for cancer treatment. This study presents a computational method that combines docking, energy scoring, and solvent-accessible surface distance filtering to generate PROTAC-compatible protein-protein interaction interfaces even in the absence of known PROTAC ligands.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2023)
Article
Engineering, Biomedical
Kayvon Javid, Carlos Fernando Mourao, Rafael Coutinho Mello-Machado, Suelen Cristina Sartoretto, Madelaine Torres, Emanuelle Stellet Lourenco, Paulo Emilio Correa Leite, Jose Mauro Granjeiro, Gutemberg Gomes Alves, Monica Diuana Calasans-Maia
Summary: This study evaluated the clinical and biochemical effects of heated serum albumin with liquid PRF (Alb-PRF) and L-PRF in socket healing after mandibular third molar extraction. The results showed that Alb-PRF significantly reduced swelling on day seven and had lower levels of pro-inflammatory cytokines compared to L-PRF. Alb-PRF promoted socket healing by reducing edema and the release of inflammatory cytokines.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Article
Materials Science, Biomaterials
Wanderson de Souza, Sara G. Piperni, Liliana Grenho, Luis A. Rocha, Jose M. Granjeiro, Sonia A. Melo, Maria H. Fernandes, Ana R. Ribeiro
Summary: Titanium (Ti) and its alloys are widely used in total joint replacement, but evidence shows that their surface degradation due to corrosion and wear processes can contribute to osteolysis and implant loosening. This study investigates the impact of titanium dioxide nanoparticles (TiO2 NPs) on osteoblast-derived exosome (Exo) biogenesis and the communication between osteoblasts and human mesenchymal stem/stromal cells (HMSCs). We found that TiO2 NPs stimulate the secretion of Exos from immature and mature osteoblasts, which have different proteomic cargo. Functional tests showed that Exos derived from both types of osteoblasts decrease the osteogenic differentiation of HMSCs. These findings have clinical relevance as wear debris can disrupt extracellular communication and lead to joint failure.
BIOMATERIALS SCIENCE
(2023)
Article
Medicine, General & Internal
Roberto Sacco, Suelen Cristina Sartoretto, Rodrigo Figueiredo de Brito Resende, Jose de Albuquerque Calasans-Maia, Alexandre Malta Rossi, Victor Hugo de Souza Lima, Carlos Fernando de Almeida Barros Mourao, Jose Mauro Granjeiro, Julian Yates, Monica Diuana Calasans-Maia
Summary: Medication-related osteonecrosis of the jaw (MRONJ) is a severe adverse side effect of certain drugs and is associated with invasive dental procedures. Previous studies have investigated preventive strategies, but no investigation has evaluated the therapeutic effect of local drug-delivery technology. This study aims to evaluate the efficacy of hydroxyapatite-containing doxycycline (HADOX) in rats with high-risk MRONJ development.
MEDICINA-LITHUANIA
(2023)
