Article
Biotechnology & Applied Microbiology
Shirong Zhou, Liang Cai, Haoqin Wu, Baoxiang Wang, Biao Gu, Song Cui, Xiaolong Huang, Zhuang Xu, Benyuan Hao, Haigang Hou, Yuan Hu, Chao Li, Yunlu Tian, Xi Liu, Liangming Chen, Shijia Liu, Ling Jiang, Jianmin Wan
Summary: This study successfully regulated the heading date of rice using CRISPR-Cas9 genome editing method, generating germplasm resources with different heading date variations by editing promoter regions and adjusting gene expression levels. Field trials were conducted to screen for elite cultivars adapted to different regions and achieve higher yield potential.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Plant Sciences
Sudip Biswas, Oneida Ibarra, Mariam Shaphek, Marco Molina-Risco, Mayra Faion-Molina, Marcela Bellinatti-Della Gracia, Michael J. Thomson, Endang M. Septiningsih
Summary: Rice is an excellent source of starch, and resistant starch (RS) in cereals has the potential to improve human health. In this study, CRISPR-Cas9 genome editing was used to simultaneously target all four SBE genes in rice, resulting in the development of high-RS lines.
Article
Plant Sciences
Yarong Lin, Yiwang Zhu, Yuchao Cui, Rui Chen, Zaijie Chen, Gang Li, Meiying Fan, Jianmin Chen, Yan Li, Xinrui Guo, Xijun Zheng, Liang Chen, Feng Wang
Summary: MicroRNAs target specific mRNA molecules based on sequence complementarity for their degradation or repression of translation. Using target mimicry (MIM) and short tandem target mimicry (STTM) can block miRNAs activity and eliminate the inhibition of their target genes, resulting in phenotypic changes. CRISPR/Cas9-mediated disruption of miRNA target sites can effectively derepress particular target genes in plants for trait improvement.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Plant Sciences
Sonia Khan Sony, Tanushri Kaul, Khaled Fathy Abdel Motelb, Arulprakash Thangaraj, Jyotsna Bharti, Rashmi Kaul, Rachana Verma, Mamta Nehra
Summary: Globally, CRISPR-Cas9-based genome editing has revolutionized crop advancements, with the development of glyphosate-resistant rice lines showing increased glyphosate resistance, enhanced aromatic amino acids, and improved grain yields. The strategy involves site-specific amino acid substitutions and modification of the OsEPSPS gene through CRISPR-Cas9-mediated genome editing. The results provide a novel weed management approach without yield penalties and a higher chance of commercial release.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Huadong Zhan, Mingmin Lu, Qin Luo, Feng Tan, Ziwei Zhao, Mingqian Liu, Yubing He
Summary: This study investigates the functional characterization of OsCPD genes in rice using CRISPR/Cas9 gene editing technology. The results show that OsCPD1 and OsCPD2 play essential and redundant roles in maintaining plant architecture by modulating BR biosynthesis, which affects the growth and development of rice.
Article
Plant Sciences
Bhuvan Pathak, Chandan Maurya, Maria C. Faria, Zahra Alizada, Soumen Nandy, Shan Zhao, Muhammed K. Jamsheer, Vibha Srivastava
Summary: This study demonstrates the efficiency of CRISPR/Cas9 in creating mutations in essential genes in rice, although the mutagenesis rates vary among different genes. Additionally, the study shows that the generated mutants exhibit phenotypes such as sterility or early senescence.
Article
Agronomy
Xinxin Liu, Hualong Liu, Yuanye Zhang, Mingliang He, Rongtian Li, Wei Meng, Zhenyu Wang, Xiufeng Li, Qingyun Bu
Summary: Through editing the uORF region of a flowering repressor in rice, the study successfully delayed the heading date, which is more time and labor saving compared to traditional breeding. In the future, uORFs of other genes related to flowering time can also be used for fine-tuning the flowering time.
Article
Agronomy
Yue Cui, Zhengjin Xu, Quan Xu
Summary: This study explores the effects of different heading mutant gene combinations on crop yield across different ecological zones. The findings suggest that adjusting the growth period of commercial varieties can optimize yield performance. By combining specific alleles, a balance between growth period and yield production can be achieved for maximum yields and improved adaptability.
MOLECULAR BREEDING
(2021)
Article
Dermatology
Huishan Shi, Jos P. H. Smits, Ellen H. van den Bogaard, Matthew G. Brewer
Summary: The CRISPR/Cas9 technology is a powerful tool used to alter genetic landscapes in various hosts, particularly in developing novel mouse lines modeling disease states. It has been successfully used in both in vitro and in vivo systems within fields like epithelial biology and dermatology. The delivery of single-guide RNA/Cas9 editing complex to host cells is crucial for its success, and different delivery techniques have been explored and evaluated for their benefits and drawbacks in genetically modifying epidermal cells. Recent advances in dermatology have utilized these methods to better understand epidermal biology, identify therapeutic targets, and develop new treatment approaches for disease states.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2021)
Article
Multidisciplinary Sciences
Xinbao Ding, Priti Singh, Kerry Schimenti, Tina N. Tran, Robert Fragoza, Jimmaline Hardy, Kyle E. Orwig, Marta Olszewska, Maciej K. Kurpisz, Alexander N. Yatsenko, Donald F. Conrad, Haiyuan Yu, John C. Schimenti
Summary: Infertility is a heterogeneous condition that can be caused by genetic factors. Genome sequencing is important for genetic diagnosis, but most rare variants found in patients have uncertain significance. Researchers evaluated the effects of these variants in key fertility genes using genome-edited mouse models and found that the predictions made by widely used algorithms were not accurate. Therefore, alternative and efficient functional validation models are needed.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Chuanhong Li, Chenbo Gong, Jiemin Wu, Linfeng Yang, Lei Zhou, Bian Wu, Liang Gao, Fei Ling, Aiqing You, Changyan Li, Yongjun Lin
Summary: In this study, thirteen type-B response regulator genes in rice were knocked out using CRISPR/Cas9 genome editing technology. The mutant materials showed variations in important traits, and further examination of the osrr30 mutant revealed delayed heading date, increased yield, and reduced chalkiness under high temperature stress. These findings suggest the potential application of type-B response regulators in rice breeding.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Xiong Liu, Yan Yu, Wei Yao, Zhongliang Yin, Yubo Wang, Zijian Huang, Jie-Qiang Zhou, Jinling Liu, Xuedan Lu, Feng Wang, Guilian Zhang, Guihua Chen, Yunhua Xiao, Huabing Deng, Wenbang Tang
Summary: Salicylic acid (SA) is an essential plant hormone that plays critical roles in plant defense. Three OsS5H homologues were found to convert SA into 2,5-dihydroxybenzoic acid (2,5-DHBA) in rice. Overexpression of OsS5H genes increased susceptibility to bacterial blight and rice blast. Triple mutants of oss5h1oss5h2oss5h3 displayed enhanced resistance to Xanthomonas oryzae pv. oryzae (Xoo) and rice blast.
PLANT BIOTECHNOLOGY JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Zhihui Chen, Hongxu Du, Yajun Tao, Yang Xu, Fangquan Wang, Bin Li, Qian-Hao Zhu, Hongbin Niu, Jie Yang
Summary: This study successfully bred rice lines with low glutelin content by mutating glutelin genes, while keeping other agronomic traits unchanged. These lines can be used as new varieties or parental materials for further breeding.
Review
Genetics & Heredity
Banita Yadav, Ashis Majhi, Kanika Phagna, Mukesh Kumar Meena, Hasthi Ram
Summary: Rice, as a major staple food crop, has been the focus of research in improving grain yield and nutritional quality. This review identifies negative regulators of grain yield and mineral contents as potential targets for CRISPR-Cas9-mediated gene knockout. Additionally, miRNAs that target positive regulators of these traits are also compiled, suggesting the potential for improving plant traits through miRNA knockout.
FUNCTIONAL & INTEGRATIVE GENOMICS
(2023)
Article
Biochemistry & Molecular Biology
Shaoyan Zheng, Chanjuan Ye, Jingqin Lu, Jiamin Liufu, Lin Lin, Zequn Dong, Jing Li, Chuxiong Zhuang
Summary: The study successfully knocked out the hexokinase gene OsHXK1 in rice using the CRISPR/Cas9 gene-editing method, resulting in increased photosynthetic efficiency and yields in multiple indica rice varieties. The findings highlight the potential of gene-editing technologies in improving rice production and shed light on the roles of OsHXK1 in regulating photosynthesis and yield.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)