期刊
RNA
卷 25, 期 4, 页码 496-506出版社
COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
DOI: 10.1261/rna.067967.118
关键词
C/D box snoRNA; small nucleolar ribonucleoprotein; small noncoding RNA; BCD1/Bcd1p (ZNHIT6); NOP58/Nop58p; Saccharomyces cerevisiae
资金
- Centre National de la Recherche Scientifique (CNRS)
- University of Lorraine (UL)
- European Union (Fond Europeen de Developpement Regional - FEDER)
- National Research Agency [ANR-11BSV8-01503, ANR-16-CE11-0032]
- Region Lorraine (Fond Europeen de Developpement Regional - FEDER)
- Agence Nationale de la Recherche (ANR) [ANR-16-CE11-0032] Funding Source: Agence Nationale de la Recherche (ANR)
Biogenesis of eukaryotic box C/D small nucleolar ribonucleoproteins (C/D snoRNPs) is guided by conserved trans-acting factors that act collectively to assemble the core proteins SNU13/Snu13, NOP58/Nop58, NOP56/Nop56, FBL/Nop1, and box C/D small nucleolar RNAs (C/D snoRNAs), in human and in yeast, respectively. This finely elaborated process involves the sequential interplay of snoRNP-related proteins and RNA through the formation of transient pre-RNP complexes. BCD1/Bcd1 protein is essential for yeast cell growth and for the specific accumulation of box C/D snoRNAs. In this work, chromatin, RNA, and protein immunoprecipitation assays revealed the ordered loading of several snoRNP-related proteins on immature and mature snoRNA species. Our results identify Bcd1p as an assembly factor of C/D snoRNP biogenesis that is likely recruited cotranscriptionally and that directs the loading of the core protein Nop58 on RNA.
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