Article
Urology & Nephrology
Erik Christensen, Inger B. Kristoffersen, Birgitte Grann, Jesper S. Thomsen, Arne Andreasen, Rikke Nielsen
Summary: Proteinuria is a well-established marker and predictor of kidney disease, and the study found that segments 1 and 2 of the proximal tubule are responsible for clearing protein filtrate in normal physiological conditions. However, under proteinuric stress, segment 3 can also participate in the reabsorption process. The endolysosomal system was most prominent in segments 1 and 2, with varied depth of ligand uptake among nephrons.
KIDNEY INTERNATIONAL
(2021)
Article
Nanoscience & Nanotechnology
Sophia Szymkowiak, Nathan Sandler, David L. Kaplan
Summary: Chronic kidney disease and kidney failure are increasing globally, with a lack of corresponding improvement in available therapies. Using silk fibroin protein scaffolds to support kidney cells can mimic the structure of the kidney and support a larger number of cells with more even distribution under perfused conditions.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Cell Biology
Shiting Liang, Youliang Wang, Meixia Kang, Juan Deng, Liting Chen, Xizhen Hong, Fan Fan Hou, Fujian Zhang
Summary: Protein reabsorption in renal proximal tubules is crucial for maintaining nutrient balance. We developed a new mouse model, AMN (CreERT2) knock-in mice, which express a fusion protein of Cre recombinase and estrogen receptor under the control of the AMN gene promoter specifically in renal proximal tubules. This model allows for the conditional knockout of genes in renal proximal tubules, providing valuable insights into their physiological function.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Oncology
Ryo Kawakami, Miki Matsui, Ayumu Konno, Ryosuke Kaneko, Shreya Shrestha, Suman Shrestha, Hiroaki Sunaga, Hirofumi Hanaoka, Sawako Goto, Michihiro Hosojima, Hideyuki Kabasawa, Masaru Obokata, Norimichi Koitabashi, Hiroki Matsui, Tsutomu Sasaki, Akihiko Saito, Motoko Yanagita, Hirokazu Hirai, Masahiko Kurabayashi, Tatsuya Iso
Summary: Urinary fatty acid binding protein 1 is identified as a biomarker for acute kidney injury, with its elevation primarily reflecting impaired protein reabsorption in proximal tubule epithelial cells.
JOURNAL OF PATHOLOGY
(2021)
Article
Multidisciplinary Sciences
Marcello Polesel, Monika Kaminska, Dominik Haenni, Milica Bugarski, Claus Schuh, Nevena Jankovic, Andres Kaech, Jose M. Mateos, Marine Berquez, Andrew M. Hall
Summary: The study visualizes the plasma protein filtration, uptake, and metabolism in the kidneys of living mice in real-time. It reveals the coordinated activity of different specialized tubular segments and major compensatory adaptations in disease states. The findings provide insights into the spatiotemporal organization of renal protein metabolism and suggest that measurement of proteinuria underestimates the severity of endocytic defects in patients.
NATURE COMMUNICATIONS
(2022)
Review
Pharmacology & Pharmacy
Marie-Louise T. Monaghan, Matthew A. Bailey, Robert J. Unwin
Summary: Historically, renal vascular and tubular function has been mainly controlled through neural and endocrine regulation. However, it is now acknowledged that complex humoral control systems exist within the kidney, complementing neuroendocrine regulation by fine-tuning renal function in response to rapid changes. The extracellular nucleotide/P2 receptor system plays a central role in intrinsic regulatory feedback loops within the kidney, contributing to the understanding and potential treatment of kidney disease.
BIOCHEMICAL PHARMACOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Rodrigo P. Silva-Aguiar, Douglas E. Teixeira, Rodrigo A. S. Peres, Diogo B. Peruchetti, Carlos P. Gomes, Alvin H. Schmaier, Patricia R. M. Rocco, Ana Acacia S. Pinheiro, Celso Caruso-Neves
Summary: Since the outbreak of COVID-19, there has been a bidirectional interaction between kidney disease and the progression of the disease. Kidney disease is an independent risk factor for mortality in COVID-19 patients and can lead to acute kidney injury (AKI) and chronic kidney disease (CKD) in these patients. However, current clinical examinations may not detect kidney damage in COVID-19 patients until an advanced stage. Studies have identified the development of subclinical acute kidney injury (subAKI) syndrome in COVID-19 patients, characterized by significant tubule interstitial injury without changes in estimated glomerular filtration rate. Changes in the protein endocytosis machinery in proximal tubule epithelial cells (PTECs) have been proposed as a potential mechanism for the development of subAKI. This paper focuses on the data relating to subAKI and COVID-19 and the role of PTECs and their protein endocytosis machinery in its pathogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Urology & Nephrology
Yasuhiro Yoshimura, Yoshiharu Muto, Kohei Omachi, Jeffrey H. Miner, Benjamin D. Humphreys
Summary: This study reveals the regulatory mechanisms of HNF4A and HNF4G during human proximal tubule differentiation. The experimental strategy can be applied more broadly to investigate transcriptional regulation in human kidney development.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2023)
Review
Urology & Nephrology
Christophe Sirac, Vecihi Batuman, Paul W. Sanders
Summary: Plasma and B cell dyscrasias causing overproduction of monoclonal immunoglobulin free light chains can affect the kidneys in various ways. The disorders may or may not meet cancer criteria and can result in a range of proximal tubule syndromes.
KIDNEY INTERNATIONAL REPORTS
(2021)
Article
Developmental Biology
Kathryn Duvall, Lauren Crist, Alison J. Perl, Naomi Pode Shakked, Praneet Chaturvedi, Raphael Kopan
Summary: Notch signaling is crucial for the maturation of nephron epithelia, with studies at both the cellular and organoid levels confirming its importance. The research found that Notch signaling plays a role in the segmentation of proximal and distal domains within the nephron.
Review
Physiology
Andrew M. Hall, Sophie de Seigneux
Summary: Damage to the proximal tubule is the most common cause of acute kidney injury in humans. Current diagnostic and treatment options are limited, and a deeper understanding of pathogenic mechanisms at a cellular level is needed. Recent studies have shown significant changes in proximal tubule metabolism during acute kidney injury, highlighting potential targets for intervention. This article emphasizes the current state of the field and highlights three emerging areas worthy of attention: axial heterogeneity in determining baseline susceptibility, insult specific pathogenic mechanisms, and the link between tubular metabolism and whole-body homeostasis.
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
(2022)
Article
Pharmacology & Pharmacy
Rodrigo A. S. Peres, Diogo B. Peruchetti, Rodrigo P. Silva-Aguiar, Douglas E. Teixeira, Carlos P. Gomes, Christina M. Takiya, Ana Acacia S. Pinheiro, Celso Caruso-Neves
Summary: This study found that rapamycin treatment can induce proteinuria, particularly affecting the handling of proteins by proximal tubule epithelial cells. In relation to this, rapamycin treatment also leads to increased proteinuria in the renal cortex. These findings suggest that the proteinuria induced by rapamycin may have a tubular origin rather than a glomerular origin.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Toxicology
Tom T. G. Nieskens, Otto Magnusson, Patrik Andersson, Magnus Soderberg, Mikael Persson, Anna-Karin Sjogren
Summary: Research demonstrated that SPC5001 ASO induced kidney injury in in vitro systems, showcasing its translational potential in the medical field.
ARCHIVES OF TOXICOLOGY
(2021)
Article
Endocrinology & Metabolism
Edoardo Biancalana, Chiara Rossi, Francesco Raggi, Mariarosaria Distaso, Domenico Trico, Simona Baldi, Ele Ferrannini, Anna Solini
Summary: This study aimed to investigate the involvement of NHE3 in modulating the response to sodium glucose co-transporter-2 inhibitors in humans. The results showed that empagliflozin acutely increased urinary pH and induced a substrate shift toward lipid utilization and ketogenesis in healthy young male volunteers, without significant changes in renal NHE3 protein expression.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2023)
Review
Peripheral Vascular Disease
Kouichi Tamura, Kengo Azushima, Sho Kinguchi, Hiromichi Wakui, Takahiro Yamaji
Summary: ATRAP is a protein that promotes AT1R internalization to inhibit its pathological activation; ATRAP is abundantly expressed in the kidney and plays a key role in regulating renal sodium handling and blood pressure modulation.
HYPERTENSION RESEARCH
(2022)
Article
Biophysics
David Choi, Zachary Gonzalez, Sum Yat Ho, Alexandra Bermudez, Neil Y. C. Lin
Summary: Epithelial cells are influenced by the mechanical and molecular cues from the substrate they reside on and their surrounding neighbors, with cell-substrate and cell-cell interactions masking each other's influence in confluent epithelia. Combinatorial changes in the physical microenvironment are necessary to alter cell morphology and gene expression.
BIOPHYSICAL JOURNAL
(2022)
Article
Multidisciplinary Sciences
Cameron A. Aubin, Benjamin Gorissen, Edoardo Milana, Philip R. Buskohl, Nathan Lazarus, Geoffrey A. Slipher, Christoph Keplinger, Josh Bongard, Fumiya Iida, Jennifer A. Lewis, Robert F. Shepherd
Summary: This article explores how system integration and multifunctionality in nature inspire a new paradigm for autonomous robots called Embodied Energy. By integrating energy directly into the structures and materials of robots, without the need for separate batteries, more efficient energy utilization can be achieved. It also introduces emerging applications of embodied energy in the development of autonomous robots.
Article
Chemistry, Multidisciplinary
John H. Ahrens, Sebastien G. M. Uzel, Mark Skylar-Scott, Mariana M. Mata, Aric Lu, Katharina T. Kroll, Jennifer A. Lewis
Summary: This study reports the fabrication of aligned cardiac tissues using bioprinting technology. By printing anisotropic organ building blocks, functional cardiac tissues with high cell density and complex cellular alignment can be generated.
ADVANCED MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Jay M. Taylor, Haiwen Luan, Jennifer A. Lewis, John A. Rogers, Ralph G. Nuzzo, Paul Braun
Summary: This article highlights recent progress in soft material chemistry and enabling methods of 3D and 4D fabrication. It discusses the emerging programmable material designs and associated assembly methods for constructing complex functional structures. The ability to programmably vary soft material architectures with properties and shapes over time is emphasized, particularly focusing on biomimetic and biologically compliant soft materials. The challenges and prospects for integrating chemistry, form, and function in this field are described, with examples of biomimetic and biologically compliant behaviors.
ADVANCED MATERIALS
(2022)
Review
Cell & Tissue Engineering
Kayla J. Wolf, Jonathan D. Weiss, Sebastien G. M. Uzel, Mark A. Skylar-Scott, Jennifer A. Lewis
Summary: The construction of human organs on demand is a solution to address the shortage of organ donors. However, replicating the complexity of native organs remains a significant challenge. The use of organ building blocks offers a pathway to create organ-specific tissues with desired organization.
Article
Materials Science, Multidisciplinary
Sebastien G. M. Uzel, Robert D. Weeks, Michael Eriksson, Dimitri Kokkinis, Jennifer A. Lewis
Summary: This paper introduces a high throughput platform called multimaterial multinozzle adaptive 3D printing (MMA-3DP), which allows for independent control of nozzle height and seamless switching between different inks. The platform is demonstrated by printing viscoelastic inks composed of different materials onto complex substrates with varying topography. This new method opens up new possibilities for rapidly patterning soft materials for structural, functional, and biomedical applications.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Article
Engineering, Biomedical
Mark A. Skylar-Scott, Jeremy Y. Huang, Aric Lu, Alex H. M. Ng, Tomoya Duenki, Songlei Liu, Lucy L. Nam, Sarita Damaraju, George M. Church, Jennifer A. Lewis
Summary: Patterned organoids and bioprinted tissues can be generated by simultaneously co-differentiating pluripotent stem cells into distinct cell types via the forced overexpression of transcription factors, independently of culture-media composition.
NATURE BIOMEDICAL ENGINEERING
(2022)
Article
Materials Science, Multidisciplinary
John J. Bowen, Shahryar Mooraj, Jacob A. Goodman, Siyuan Peng, Dayton P. Street, Benito Roman-Manso, Emily C. Davidson, Kara L. Martin, Lisa M. Rueschhoff, Scott N. Schiffres, Wen Chen, Jennifer A. Lewis, Matthew B. Dickerson
Summary: This article presents a method that combines self-assembly and 3D printing to create hierarchically porous ceramic architectures. By utilizing additive manufacturing and nanoscale porosity generation, ceramic lattices with excellent mechanical energy absorption and low thermal conductivity can be produced.
Review
Medical Laboratory Technology
Murat Tekguc, Ronald C. Van Gaal, Sebastien G. M. Uzel, Navin Gupta, Leonardov V. Riella, Jennifer A. Lewis, Ryuji Morizane
Summary: The kidney is an important organ that plays a crucial role in regulating bodily fluid and electrolyte balance. Traditional research methods are unable to fully replicate complex human kidney diseases. However, advancements in directed differentiation of induced pluripotent stem cells and biofabrication techniques have allowed the generation of patient-specific 3D kidney organoids and the construction of vascularized kidney tissues. Gene editing technology can also be used to minimize immune rejection in kidney transplant recipients.
TRANSLATIONAL RESEARCH
(2022)
Article
Chemistry, Multidisciplinary
Benito Roman-Manso, Robert D. Weeks, Ryan L. Truby, Jennifer A. Lewis
Summary: Embedded 3D printing combined with microwave-activated curing is used to generate architected ceramics with spatially controlled composition in freeform shapes. This integrated manufacturing method opens new avenues for the design and fabrication of complex ceramic architectures with programmed composition, density, and form for myriad applications.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Robert D. D. Weeks, Ryan L. L. Truby, Sebastien G. M. Uzel, Jennifer A. A. Lewis
Summary: Recent advancements in computational design and 3D printing technology have allowed for the fabrication of polymer lattices that have a high strength-to-weight ratio and tailored mechanics. However, limitations with most commercial 3D printing platforms have only allowed for the construction of monolithic 3D lattices. This study demonstrates the use of embedded three-dimensional (EMB3D) printing to create multi-material polymer lattices, enabling the fabrication of a wide range of periodic and stochastic lattices with tailored mechanical responses by printing multiple materials with different mechanical properties.
ADVANCED MATERIALS
(2023)
Article
Multidisciplinary Sciences
Natalie M. Larson, Jochen Mueller, Alex Chortos, Zoey S. Davidson, David R. Clarke, Jennifer A. Lewis
Summary: This article presents a rotational multimaterial 3D printing (RM-3DP) platform that allows subvoxel control over the local orientation of azimuthally heterogeneous architected filaments. By continuously rotating a multimaterial nozzle with a controlled ratio of angular-to-translational velocity, helical filaments with programmable helix angle, layer thickness, and interfacial area between different materials can be created. Functional artificial muscles and hierarchical lattices composed of architected helical struts have been successfully fabricated using this method.
Article
Materials Science, Multidisciplinary
Bradley Duncan, Robert D. Weeks, Benjamin Barclay, Devon Beck, Patrick Bluem, Roberto Rojas, Maxwell Plaut, John Russo, Sebastien G. M. Uzel, Jennifer A. Lewis, Theodore Fedynyshyn
Summary: In this study, low-loss graded dielectric materials were successfully designed and printed using active mixing of nanocomposite inks. This method enables the rapid fabrication of high-performance RF devices with locally tunable dielectric properties.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Cell Biology
Rolando Carrisoza-Gaytan, Katharina T. Kroll, Ken Hiratsuka, Navin R. Gupta, Ryuji Morizane, Jennifer A. Lewis, Lisa M. Satlin
Summary: Kidney organoids cultured with flow exhibit more mature podocytes and tubules compared to static controls. However, their physiological function needs further investigation.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Materials Science, Multidisciplinary
Connor A. Verheyen, Sebastien G. M. Uzel, Armand Kurum, Ellen T. Roche, Jennifer A. Lewis
Summary: This study combines experimentation and computation to design granular matrices with controlled properties, allowing for potential applications in cell encapsulation, bioprinting, and tissue engineering.
