Article
Pharmacology & Pharmacy
Hiroyasu Sakai, Yuta Suzuki, Yu Miyauchi, Fumiaki Sato, Yusuke Ando, Risako Kon, Nobutomo Ikarashi, Yoshihiko Chiba, Junzo Kamei, Tomoo Hosoe
Summary: Cancer patients often experience muscle atrophy, which is worsened by the decreased muscle regenerative capacity. Cisplatin, a cancer chemotherapeutic agent, has been found to cause muscle atrophy. This study explored the effects of cisplatin on the differentiation of myoblasts into myotubes. The results showed that cisplatin suppresses myogenesis and inhibits the expression of Sparcl1, which promotes C2C12 cell differentiation during myogenesis.
BIOCHEMICAL PHARMACOLOGY
(2022)
Article
Cell Biology
Xiaoli Liu, Dongyue Zhang, Hao Wang, Qian Ren, Lina Wang, Guoguang Zheng
Summary: The study revealed the heterogeneity of miRNA expression profiles in LAMs, and showed that overexpression of miR-451a can alter the morphology of macrophages, enhance their phagocytic ability, and promote the expression of differentiation marker CD11b. Moreover, miR-451a was found to increase the proliferation capacity of both M1- and M2-polarized macrophages.
CELLULAR IMMUNOLOGY
(2021)
Article
Medicine, Research & Experimental
Wojciech Francuzik, Kristijan Pazur, Magdalena Dalke, Sabine Doelle-Bierke, Magda Babina, Margitta Worm
Summary: This study aimed to investigate serum biomarker profiles (proteins, lipids, and miRNAs) to support the diagnosis of anaphylaxis. It was found that hsa-miR-451a was elevated during anaphylaxis and could serve as a significant biomarker. Additionally, combining multiple biomarkers improved the accuracy of diagnosing patients with anaphylaxis.
Article
Cell Biology
Guoping Shi, Dan Li, Dongya Zhang, Yujun Xu, Yuchen Pan, Li Lu, Jingman Li, Xiaoyu Xia, Huan Dou, Yayi Hou
Summary: This study found that the percentage of M-MDSCs increased in pristane-induced lupus mice, and the differentiation of M-MDSCs was promoted by Toll-like receptor 7 signal activation and high interferon-alpha levels. Additionally, the AMPK agonist metformin and mTOR inhibitors were found to inhibit M-MDSC percentages in lupus mice as well as in vitro differentiation into MDSCs induced by TLR7 and IFN-alpha.
CELL DEATH DISCOVERY
(2021)
Article
Dermatology
Jae Kyung Lee, Soo-Jin Oh, Jeong-An Gim, Ok Sarah Shin
Summary: This study uncovered miR-10a, miR-30c, and miR-451a as pro-aging factors in small extracellular vesicles (sEV) of senescent human dermal fibroblasts (HDFs), demonstrating the crucial role of sEV miRNAs in aging processes. Over-expression of these miRNAs induced cellular senescence, while inhibition of these miRNAs reduced senescent phenotypes. Interestingly, sEV miRNAs were found to regulate ROS production and mitophagy activation.
JOURNAL OF INVESTIGATIVE DERMATOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Vidyadharan Geetha Manasa, Shaji Thomas, Sankarareddiar Kannan
Summary: MiR-144 and miR-451a are downregulated in oral squamous cell carcinoma cell lines, and their co-expression significantly reduces the migratory, invasive, and clonogenic potential of cells. This suggests that the miR-144/451a cluster functions as a tumor suppressor in OSCC.
Article
Dermatology
J. Nousbeck, M. A. McAleer, G. Hurault, E. Kenny, K. Harte, S. Kezic, R. J. Tanaka, A. D. Irvine
Summary: The study found that infants with atopic dermatitis (AD) have a distinct microRNA signature in different blood compartments, with miR-451a identified as a promising novel biomarker for the early diagnosis of AD.
BRITISH JOURNAL OF DERMATOLOGY
(2021)
Article
Oncology
Wan Fu, Guangcai Yu, Junnan Liang, Pan Fan, Keshuai Dong, Bixiang Zhang, Xiaoping Chen, Hong Zhu, Liang Chu
Summary: The study demonstrated that miR-144-5p and miR-451a are significantly decreased in cholangiocarcinoma and their downregulation is associated with disease progression. These two miRNAs suppress the proliferation, invasion, and migration of cholangiocarcinoma cells by regulating the expression of ST8SIA4, indicating a tumor suppressor role in cholangiocarcinoma.
FRONTIERS IN ONCOLOGY
(2021)
Article
Nutrition & Dietetics
Mariana S. Dorna, Elizabete M. S. Barbosa, Matheus A. Callegari, Suzana E. Tanni, Fernanda Chiuso-Minicucci, Tainara F. Felix, Ana L. Seneda, Camila R. Correa, Ana A. H. Fernandes, Paula S. Azevedo, Bertha F. Polegato, Marcelo M. Rogero, Sergio A. R. Paiva, Leonardo A. M. Zornoff, Patricia P. Reis, Marcos F. Minicucci
Summary: The study found that the expression of circulating miR-150-5p, miR-25-3p, and miR-451a increased after smoking, but was attenuated after consuming orange juice. While serum superoxide dismutase and glutathione peroxidase activity increased after orange juice intake, there were no changes in lipid hydroperoxide levels.
FRONTIERS IN NUTRITION
(2021)
Article
Gastroenterology & Hepatology
Noriaki Murayama, Koichi Okamoto, Tadahiko Nakagawa, Jinsei Miyoshi, Kensei Nishida, Tomoyuki Kawaguchi, Kaizo Kagemoto, Shinji Kitamura, Beibei Ma, Hiroshi Miyamoto, Naoki Muguruma, Mitsuyasu Yano, Koichi Tsuneyama, Takahiro Fujimori, Yasushi Sato, Tetsuji Takayama
Summary: The overexpression of miR-144/miR-451 cluster promotes lymphovascular invasion in rectal NET-G1 cells by repressing PTEN and p19. The miR-144/451 cluster may be a novel biomarker for predicting invasion/metastasis in rectal NET-G1.
JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Warren B. Nothnick, Riley Peterson, Paige Minchella, Tommaso Falcone, Amanda Graham, Austin Findley
Summary: This study found an inverse relationship between miR-451a, miR-25-3p, and PTEN. In vitro cell experiments suggested that miR-451a may regulate PTEN expression via modulating miR-25-3p.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Xu Dong, Jiabao Xing, Qingchun Liu, Mao Ye, Zhen Zhou, Yantao Li, Rongqin Huang, Zhenhui Li, Qinghua Nie
Summary: In this study, a circRNA-miRNA-mRNA interaction network was identified in chicken primary myoblasts' myogenesis. The circPLXNA2-gga-miR-12207-5P-MDM4 axis was found to play a critical role in regulating myogenesis by inhibiting apoptosis and promoting cell proliferation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Endocrinology & Metabolism
Meng Li, Feng Xu, Zhongxian Liu, Chunguang Wang, Yun Zhao, Guoping Zhu, Xiaopeng Shen
Summary: The research indicates that TNF signaling mediates myogenesis defects in DM1 and may regulate myogenesis in DM1 downstream of miR-322/-503. Inhibiting TNF signaling benefits myogenesis defects in DM1, providing a novel therapeutic strategy for the disease.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Mai Thi Nguyen, Kyung-Ho Min, Wan Lee
Summary: This study revealed the critical role of miR-429-3p in myogenic differentiation by suppressing CFL2, providing implications of SFA-induced miRNA in regulating actin dynamics and skeletal myogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Han Jin Cho, Young-Sun Lee, Da Ae Kim, Sung Ah Moon, Seung Eun Lee, Seung Hun Lee, Jung-Min Koh
Summary: This study found that Lumican is a soluble factor secreted by muscles that promotes myogenesis and protects against muscle loss by activating p38 MAPK via integrin receptors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Tanvi Sinha, Chirag Panigrahi, Debojyoti Das, Amaresh Chandra Panda
Summary: Most RNA molecules in cells are noncoding RNAs, while a small percentage of circRNAs have the potential to translate into proteins through a cap-independent translation initiation mechanism. Studying circRNA translation will uncover the hidden proteome and its therapeutic implications in human health.
WILEY INTERDISCIPLINARY REVIEWS-RNA
(2022)
Review
Cell Biology
Suman Singh, Sharmishtha Shyamal, Amaresh C. Panda
Summary: In the past decade, there has been a significant increase in novel RNA molecules and their complexity in gene regulation. RNA-RNA interactions play a critical role in cellular events by regulating the biogenesis and function of RNA molecules. Understanding the mechanisms and functions of RNA-RNA interactions is essential for eukaryotes. The advent of high-throughput RNA-sequencing technologies has revolutionized the study of RNA-RNA interactions.
WILEY INTERDISCIPLINARY REVIEWS-RNA
(2022)
Article
Multidisciplinary Sciences
Arundhati Das, Debojyoti Das, Amaresh C. Panda
Summary: Digital droplet polymerase chain reaction (dd-PCR) is a highly sensitive quantification method that accurately measures the levels of specific circular RNA (circRNA) in cells. circRNAs regulate gene expression by acting as sponges for RNA-binding proteins and microRNAs, and they can serve as biomarkers for disease diagnosis.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Correction
Multidisciplinary Sciences
Carlos Anerillas, Allison B. Herman, Rachel Munk, Amanda Garrido, Kwan-Wood Gabriel Lam, Matthew J. Payea, Martina Rossi, Dimitrios Tsitsipatis, Jennifer L. Martindale, Yulan Piao, Krystyna Mazan-Mamczarz, Jinshui Fan, Chang-Yi Cui, Supriyo De, Kotb Abdelmohsen, Rafael de Cabo, Myriam Gorospe
NATURE COMMUNICATIONS
(2022)
Article
Cell Biology
Aniruddha Das, Tanvi Sinha, Smruti Sambhav Mishra, Debojyoti Das, Amaresh C. Panda
Summary: This study analyzed previously published HeLa cell RNA-seq datasets to identify circRNA splice variants using the de novo module of the CIRCexplorer2 circRNA annotation pipeline. A subset of validated circRNAs were predicted to translate into proteins and their association with polyribosome was confirmed. Bioinformatics analysis of proteins derived from circRNA splice variants suggested altered protein sequences and structures that could affect their physiological functions.
EUROPEAN JOURNAL OF CELL BIOLOGY
(2023)
Article
Geriatrics & Gerontology
Sara E. Espinoza, Sundeep Khosla, Joseph A. Baur, Rafael de Cabo, Nicolas Musi
Summary: The geroscience hypothesis suggests that targeting key hallmarks of aging can improve healthspan and prevent age-related diseases. Several pharmacological interventions, including senolytics, NAD(+) boosters, and metformin, are being studied for their potential benefits. Preclinical studies show that senolytic drugs improve healthspan in rodents, while increasing NAD(+) through supplementation appears to extend healthspan in model organisms. Metformin, on the other hand, has pleiotropic effects and is being examined for its potential to improve healthspan and prevent frailty in clinical trials. However, further research is needed to determine their efficacy, safety, target populations, and long-term outcomes.
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES
(2023)
Article
Biology
Martina Rossi, Carlos Anerillas, Maria Laura Idda, Rachel Munk, Chang Hoon Shin, Stefano Donega, Dimitrios Tsitsipatis, Allison B. Herman, Jennifer L. Martindale, Xiaoling Yang, Yulan Piao, Krystyna Mazan-Mamczarz, Jinshui Fan, Luigi Ferrucci, Peter F. Johnson, Supriyo De, Kotb Abdelmohsen, Myriam Gorospe, Berenice A. Benayoun
Summary: Senescent cells release the senescence-associated secretory phenotype (SASP), which is a collection of cytokines, proteases, and growth factors associated with chronic inflammation and age-related diseases. This study explored the expression and role of the immunomodulatory cytokine BAFF in different senescence models. BAFF production was found to be elevated in various senescence paradigms, and the transcription factor IRF1 was identified as necessary for TNFSF13B mRNA transcription. Inhibition of BAFF production reduced the senescent phenotype and secretion of IL6 in fibroblasts and monocyte-like cells, with cell type-specific effects on NF-kappa B activation and TP53 function.
Article
Cell Biology
Jayanta Kumar Das, Nirad Banskota, Julian Candia, Michael E. Griswold, Melissa Orenduff, Rafael de Cabo, David L. Corcoran, Sai Krupa Das, Supriyo De, Kim Marie Huffman, Virginia B. Kraus, William E. Kraus, Corby K. Martin, Susan B. Racette, Leanne M. Redman, Birgit Schilling, Daniel W. Belsky, Luigi Ferrucci
Summary: Using high-depth RNA-Seq analysis, this study found that caloric restriction (CR) positively affects skeletal muscle quality in humans and alters the gene expression and splicing profiles of biological pathways affected by CR in model organisms.
Review
Cell Biology
Suman Singh, Tanvi Sinha, Amaresh C. Panda
Summary: Circular RNAs have emerged as novel regulators of gene expression through various mechanisms. Most studies focus on functional circRNAs regulating target gene expression by interacting with microRNAs. Although computational tools and molecular assays have been used to predict and validate the interaction of miRNAs with circRNAs, careful validation of functional interactions in vivo is necessary.
WILEY INTERDISCIPLINARY REVIEWS-RNA
(2023)
Article
Cell Biology
Noah Wechter, Martina Rossi, Carlos Anerillas, Dimitrios Tsitsipatis, Yulan Piao, Jinshui Fan, Jennifer L. Martindale, Supriyo De, Krystyna Mazan-Mamczarz, Myriam Gorospe
Summary: Senescence is a state of enduring growth arrest triggered by sublethal cell damage, and the accumulation of senescent cells in older individuals has been linked to age-related diseases. However, identifying robust markers of senescence has been challenging due to the heterogeneous and dynamic nature of the senescent phenotype. In this study, single-cell RNA-sequencing analysis was used to comprehensively analyze the senescent transcriptome of human diploid fibroblasts, revealing distinct cell subpopulations and dynamic changes in gene expression during senescence. This research provides a deeper understanding of senescent cell phenotypes and may lead to the development of more effective interventions.
Article
Cell Biology
Hitoshi Watanabe, Wen Du, Jinsook Son, Lina Sui, Shun-ichiro Asahara, Irwin J. Kurland, Taiyi Kuo, Takumi Kitamoto, Yasutaka Miyachi, Rafael de Cabo, Domenico Accili
Summary: Sulfonylureas are effective and affordable antidiabetic drugs, but chronic use leads to secondary failure. Cytochrome b5 reductase 3 (Cyb5r3) down-regulation is identified as a mechanism of secondary SU failure and can be successfully reversed. Cyb5r3 activators can rescue secondary SU failure and potentially rehabilitate SU use in diabetes.
SCIENCE TRANSLATIONAL MEDICINE
(2023)
Article
Cell Biology
Magdalena Juhaszova, Evgeny Kobrinsky, Dmitry B. Zorov, H. Bradley Nuss, Yael Yaniv, Kenneth W. Fishbein, Rafael de Cabo, Lluis Montoliu, Sandra B. Gabelli, Miguel A. Aon, Sonia Cortassa, Steven J. Sollott
Summary: ATP synthase can utilize both Delta psi(m)-driven H+- and K+-transport to synthesize ATP under physiological conditions. The presence of K+ increases ATP synthesis rate in isolated mitochondria while also increasing oxygen consumption rate. The data obtained from purified F1Fo single molecule experiments are consistent with the functional data observed in intact mitochondria.
Article
Cell Biology
Magdalena Juhaszova, Evgeny Kobrinsky, Dmitry B. Zorov, H. Bradley Nuss, Yael Yaniv, Kenneth W. Fishbein, Rafael de Cabo, Lluis Montoliu, Sandra B. Gabelli, Miguel A. Aon, Sonia Cortassa, Steven J. Sollott
Summary: ATP synthase serves as the primary way for K+ to enter mitochondria, and its activity can be upregulated by endogenous survival-related proteins via IF1. The interaction between IF1 and Bcl-xL or Mcl-1 enhances ATP synthase's efficiency and limits ischemia-reperfusion injury.