Radicle emergence with increased temperatures following summer dispersal in Trillium camschatcense: A species with deep simple double morphophysiological dormancy in seeds

Seeds with deep simple double morphophysiological dormancy (MPD) need cold stratification during the first winter after dispersal for radicle emergence, followed by the summer for root and bud development and finally a second winter for shoot emergence. In a previous study, we demonstrated that Trillium camschatcense seeds have this type of dormancy with radicles emerging from most seeds after the first winter. However, radicles also emerged from a few seeds in autumn during the same year as dispersal. We thought that temperatures after seed dispersal played a role in radicle emergence before the first winter. To confirm our idea, we investigated germination phenology outdoors, relationships between temperatures after seed dispersal and radicle emergence in the first year outdoors, radicle emergence in the first winter under varied temperatures using incubators, and shoot emergence from seeds with an emerged radicle in the first year outdoors. Our phenology study confirmed that T. camschatcense seeds have deep simple double MPD. Over 7 years, 0.2-7.5% of radicles emerged in the first year before winter and these percentages were moderately positively correlated with temperatures, especially minimum temperatures. Increasing August and September temperatures increased radicle emergence in the laboratory. Shoots emerged from seeds with an emerged radicle in the first year after the first winter. With increased autumn temperatures in warmer regions or with global warming, we predict that germination phenology may shift: increased radicle emergence in the first year and shoot emergence following the first (and not second) winter.