Article
Cell Biology
Ke Xu, Jing-Yi Qiao, Bin-Wang Zhao, Ming-Zhe Dong, Wen-Long Lei, Yuan-Yuan Li, Zhenyu Ju, Heide Schatten, Zhen-Bo Wang, Kui Liu, Qing-Yuan Sun
Summary: The role of SMC2, a core component of condensin complex, in oocyte and embryo development was investigated. Deletion of SMC2 in oocytes caused female infertility, abnormal chromosome condensation, DNA damage, and impaired pronuclear function in fertilized eggs, leading to arrested embryo development at the one-cell stage.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Xuyan He, Chaodong Ge, Jun Xia, Zhidan Xia, Lu Zhao, Sicong Huang, Rong Wang, Jianwei Pan, Tao Cheng, Peng-Fei Xu, Fudi Wang, Junxia Min
Summary: SLC39A10 is identified as a key zinc transporter in hematopoiesis, and it plays a larger role than iron in early hematopoietic stem cells. Loss of Slc39a10 causes zinc deficiency, leading to DNA damage, apoptosis, and cell cycle arrest. Zinc supplementation and inhibiting necroptosis were found to restore hematopoiesis, suggesting the potential of SLC39A10 as a therapeutic target for anemia and zinc deficiency-related disorders.
Article
Developmental Biology
Mudan He, Shengbo Jiao, Ru Zhang, Ding Ye, Houpeng Wang, Yonghua Sun
Summary: This study demonstrates that maternal Nanog regulates oogenesis and early embryogenesis through translational control of maternal mRNA via a mechanism whereby Nanog acts as a transcriptional repressor to suppress transcription of eef1a1l2. The loss of Nanog leads to defects in egg maturation and increased translational activity, which can be rescued by depleting eef1a1l2.
Article
Multidisciplinary Sciences
Erika E. Paulson, Emily L. Fishman, Richard M. Schultz, Pablo J. Ross
Summary: MicroRNAs play a crucial role in preimplantation embryo development, with their expression increasing during the morula and blastocyst stages. Knockdown of DGCR8 inhibits the expression of embryonically expressed miRNAs and the morula-to-blastocyst transition.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Multidisciplinary Sciences
Ting Zhao, Jingyun Lu, Huairen Zhang, Mande Xue, Jie Pan, Lijun Ma, Frederic Berger, Danhua Jiang
Summary: This study reveals the crucial role of Arabidopsis histone variant H3.3 in endowing seeds with post-embryonic developmental potentials and regulating germination and post-embryonic development. H3.3 facilitates chromatin opening at regulatory regions, regulates gene transcriptional regulation during germination, and is associated with gene body DNA methylation and the restriction of chromatin accessibility and cryptic transcription at specific gene ends in seeds.
NATURE COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Jacinta I. Kalisch-Smith, Nikita Ved, Dorota Szumska, Jacob Munro, Michael Troup, Shelley E. Harris, Helena Rodriguez-Caro, Aimee Jacquemot, Jack J. Miller, Eleanor M. Stuart, Magda Wolna, Emily Hardman, Fabrice Prin, Eva Lana-Elola, Rifdat Aoidi, Elizabeth M. C. Fisher, Victor L. J. Tybulewicz, Timothy J. Mohun, Samira Lakhal-Littleton, Sarah De Val, Eleni Giannoulatou, Duncan B. Sparrow
Summary: Congenital heart disease is a common human birth defect with many cases potentially caused by maternal iron deficiency, which can be prevented by iron administration in early pregnancy. Genetic predisposition and environmental factors may interact to impact the development of heart disease.
NATURE COMMUNICATIONS
(2021)
Article
Developmental Biology
Holly N. Stephenson, Robert Streeck, Florian Grublinger, Christian Goosmann, Alf Herzig
Summary: Hemocytes play a crucial role in post-embryonic development in Drosophila, beyond their known function in controlling microbiota. Ablation of hemocytes leads to dysregulation of the midgut and reduced gut lengths, affecting multiple processes during metamorphosis.
Article
Genetics & Heredity
Qiting Yao, Shi Duan, Qing Yang, Xiaoqin Ma, Zhenyu Li, Kongyue Wu, Pei Chang, Mengxi Cao, Xinhua Chen, Zequn Wang, Xueping Zhong, Qingchun Zhou, Haobin Zhao
Summary: This study reveals the critical role of Mep50 in medaka embryonic development. Loss of Mep50 leads to abnormal embryogenesis and affects the maintenance of stem cells and inhibition of apoptosis.
Article
Cell Biology
Zhaohui Wei, Qiang Hong, Zijiao Ding, Jingwen Liu
Summary: The study generated mutant lines for cxcl12a and cxcr4b using the CRISPR/Cas9 system. Results showed that Cxcl12a is crucial for pharyngeal cartilage formation by promoting the proliferation of craniofacial neural crest cells. Additionally, Cxcl12a and Cxcl12b were found to have a synergistic influence on pharyngeal arch and pouch formation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Alexander Vietheer, Torvid Kiserud, Oystein Ariansen Haaland, Rolv Terje Lie, Joerg Kessler
Summary: Sleep duration before pregnancy has an impact on fetal development, with differences seen in yolk sac and crown-rump-length measurements in male fetuses but not female fetuses at 7 weeks. These findings suggest a sex- and time-dependent embryonic adaptation to sleep affecting the intrauterine environment in normal pregnancies.
SCIENTIFIC REPORTS
(2022)
Article
Plant Sciences
Ori Avraham Erlichman, Shahar Weiss, Maria Abu Arkia, Moria Ankary-Khaner, Yoram Soroka, Weronika Jasinska, Leah Rosental, Yariv Brotman, Tamar Avin-Wittenberg
Summary: Seeds are important for providing nutrients and promoting germination. Autophagy plays a crucial role in seed development, affecting nutrient remobilization, protein accumulation, seed coat morphology, and germination. It is necessary to examine autophagy in a tissue-specific manner in order to understand its functions during seed development.
Article
Biochemistry & Molecular Biology
Adda-Lee Graham-Paquin, Deepak Saini, Jacinthe Sirois, Ishtiaque Hossain, Megan S. Katz, Qinwei Kim-Wee Zhuang, Sin Young Kwon, Yojiro Yamanaka, Guillaume Bourque, Maxime Bouchard, William A. Pastor
Summary: Zmym2(-/-) mice exhibit embryonic lethality and fail to undergo proper DNA methylation, resulting in upregulation of germline genes and abnormal expression of transposon-gene fusion transcripts. It suggests that ZMYM2 plays a crucial role in early embryonic development and DNA methylation patterning.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Plant Sciences
Nathaniel Perry, Colin D. Leasure, Hongyun Tong, Elias M. Duarte, Zheng-Hui He
Summary: The Arabidopsis RUS gene family contains six members, with RUS6 being essential for early embryo development. The RUS gene family is involved in multiple stages of plant development.
Article
Multidisciplinary Sciences
Erdyni N. Tsitsikov, Khanh P. Phan, Yufeng Liu, Alla V. Tsytsykova, Mike Kinter, Lauren Selland, Lori Garman, Courtney Griffin, Ian F. Dunn
Summary: TRAF7 is a crucial molecule involved in the shear stress-induced MEKK3-MEK5-ERK5 signaling pathway in endothelial cells. It associates with SCRIB and may be an upstream component of the signaling pathway.
Article
Obstetrics & Gynecology
Pooja Popli, Vinay Shukla, Jyoti B. Kaushal, Rohit Kumar, Kanchan Gupta, Anila Dwivedi
Summary: This study identified the secretory protein PRDX6 in the rabbit oviduct and demonstrated its role in early pregnancy events, fertilization, and embryo development. PRDX6 expression was significantly higher in mated animals compared to non-mated controls, and reduction of PRDX6 led to decreased embryo numbers and impaired early embryonic development. Silencing of PRDX6 induced oxidative stress and inflammation in oviduct cells, creating a microenvironment detrimental to embryo development. These findings suggest that PRDX6 plays a crucial role in maintaining an optimal microenvironment for successful embryo development and may have therapeutic potential in IVF strategies.
REPRODUCTIVE SCIENCES
(2022)
Article
Agronomy
Yu Han, Si-Da Zhou, Jiong-Jiong Fan, Lei Zhou, Qiang-Sheng Shi, Yan-Fei Zhang, Xing-Lu Liu, Xing Chen, Jun Zhu, Zhong-Nan Yang
Summary: Overall, OsMS188 is identified as a key regulator of tapetum development and pollen wall formation in rice. The gene regulatory network established in this study sheds light on future investigations of fertility regulation in rice and other crop species.
Article
Biochemistry & Molecular Biology
Kai-Qi Wang, Ya-Hui Yu, Xin-Lei Jia, Si-Da Zhou, Fang Zhang, Xin Zhao, Ming-Yue Zhai, Yi Gong, Jie-Yang Lu, Yuyi Guo, Nai-Ying Yang, Shui Wang, Xiao-Feng Xu, Zhong-Nan Yang
Summary: Understanding photoperiod/temperature-sensitive genic male sterility (P/TGMS) is important for improving crop production. This study identified a gene, res3, that can restore male sterility and investigated its mechanism of action. The findings suggest that res3 plays a role in tapetum secretory function and can compensate for pollen wall defects in other P/TGMS lines. These results provide valuable insights into the cellular mechanism of fertility restoration in P/TGMS lines.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2022)
Article
Plant Sciences
Yue Jin, Xinyue Song, Huizhong Chang, Yueyue Zhao, Chenhao Cao, Xinbao Qiu, Jun Zhu, Ertao Wang, Zhongnan Yang, Nan Yu
Summary: Pollen protects male sperm and regulates anther development, allowing flowering plants to adapt to different terrestrial environments. The SLR1 gene in rice plays a crucial role in anther development, mainly expressed in tapetal cells and tetrads, and interacts with OsMS188 to regulate pollen wall formation.
Article
Plant Sciences
Cheng Zhang, Meng-Yi Ren, Wen-Jian Han, Ya-Fei Zhang, Min-Jia Huang, Si-Yuan Wu, Jie Huang, Yun Wang, Zheng Zhang, Zhong-Nan Yang
Summary: Slow development has been shown to restore the fertility of thermo-sensitive and photoperiod-sensitive genic male sterile lines in Arabidopsis, with RPG1 and RPG2 playing redundant roles in fertility restoration. Under slow growth conditions, primexine formation in rpg1 is partially restored, leading to fertility recovery. Additionally, RPG2 is essential for fertility restoration in rpg1, with reduced fertility observed under low temperature conditions. The study highlights the importance of gene redundancy in fertility restoration for TGMS lines and provides insights into pollen wall pattern formation.
Article
Plant Sciences
Zaibao Zhang, Huadong Zhan, Jieyang Lu, Shuangxi Xiong, Naiying Yang, Hongyu Yuan, Zhong-Nan Yang
Summary: The KCS family members expressed in anther play crucial roles in the accumulation of pollen coat lipids, particularly KCS7, KCS15, and KCS21 expressed in tapetal cells. These proteins are involved in pollen-stigma interaction by affecting the hydration and pollen tube growth, revealing the importance of tapetal-derived pollen coat lipids.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Yanfei Zhang, Chao Chen, Zexin Jin, Zhongnan Yang, Yueling Li
Summary: This study investigated the effects of light intensity on the morphology, photosynthesis, chloroplast ultrastructure, and chloroplast gene expression of Heptacodium miconioides (H. miconioides). The research found that light intensity affected leaf morphology, chloroplast development, and photosynthetic efficiency.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2022)
Article
Biochemistry & Molecular Biology
Yilu Wang, Yinhua Lu, Jieyang Lu, Zhong-Nan Yang, Zhongyi Yang
Summary: Ethylenediamine-N,N'-disuccinic acid [(S,S)-EDDS] is a biodegradable strong chelating agent that can currently be produced by biological methods, including fermentation and enzymatic synthesis. Enzymatic methods have a clearer mechanism than fermentation and are more economical and environmentally friendly.
PROCESS BIOCHEMISTRY
(2022)
Article
Plant Sciences
Zi-Yuan Wang, Wan-Tong Qu, Tong Mei, Nan Zhang, Nai-Ying Yang, Xiao-Feng Xu, Hai-Bo Xiong, Zhong-Nan Yang, Qing-Bo Yu
Summary: This study further analyzed the function of AtRsmD in chloroplast development and photosynthesis, revealing its distinct regulatory roles in chloroplast translation.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Nai-Ying Yang, Xin-Lei Jia, Chang-Xu Sui, Shi-Yi Shen, Xi-Ling Dai, Jing-Shi Xue, Zhong-Nan Yang
Summary: Reconstructing the development of sporangia in seed-free vascular plants is crucial for understanding spore production. This study used fluorescence imaging and confocal microscopy to reconstruct the key steps of sporangia development, with a focus on the cell wall structures of tapetum and spore mother cells. By staining the cell wall and cytoplasm, the researchers observed the development of sporangia in Pteris multifida and revealed the clear cell lineages involved. This work improves our overall understanding of fern spore formation.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Hai-Bo Xiong, Hui-Min Pan, Qiao-Ying Long, Zi-Yuan Wang, Wan-Tong Qu, Tong Mei, Nan Zhang, Xiao-Feng Xu, Zhong-Nan Yang, Qing-Bo Yu
Summary: This study reveals the mechanism of transcription-translation coupling in chloroplasts and finds that AtNusG-mediated coupling contributes to the establishment of photosynthetic capacity and response to environmental changes in plants.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biotechnology & Applied Microbiology
Yan-Fei Zhang, Yue-Ling Li, Xiang Zhong, Jun-Jie Wang, Lei Zhou, Yu Han, Dan-Dan Li, Na Wang, Xue-Hui Huang, Jun Zhu, Zhong-Nan Yang
Summary: A novel rice TGMS line, ostms18, was identified in this study, showing more stable sterility in summer compared to the widely used tms5 locus. The TGMS trait of ostms18 is caused by a point mutation in a GMC oxidoreductase gene, resulting in defective pollen wall formation under high temperature but restoration of fertility under low temperature.
PLANT BIOTECHNOLOGY JOURNAL
(2022)
Article
Cell Biology
Ben-Shun Zhu, Ying-Xiu Zhu, Yan-Fei Zhang, Xiang Zhong, Keng-Yu Pan, Yu Jiang, Chi-Kuang Wen, Zhong-Nan Yang, Xiaozhen Yao
Summary: Ethylene inhibits anther development through the EIN2-EIN3/EIL1 signaling pathway, leading to pollen abortion.
Article
Biochemistry & Molecular Biology
Chong Wang, Hao Cheng, Wenjing Xu, Jingshi Xue, Xinguo Hua, Guimin Tong, Xujun Ma, Chuanping Yang, Xingguo Lan, Shi-Yi Shen, Zhongnan Yang, Jirong Huang, Yuxiang Cheng
Summary: This study reveals the roles of pollen-specific GDPD-LIKE genes in pollen tube tip growth. The GDPD-LIKE genes mediate pollen tube tip growth by modulating cellulose deposition in pollen tube walls. These findings are important for understanding the mechanism of pollen tube growth and seed formation.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2023)
Article
Plant Sciences
Yunxia He, Xiaomeng He, Xiaoyang Wang, Mengyue Hao, Jiale Gao, Yangxiayu Wang, Zhong-Nan Yang, Xiangzong Meng
Summary: EPFL4, EPFL5, and EPFL6 peptides promote stamen filament elongation by enhancing filament cell proliferation. Loss of EPFL4-6 genes results in defective filament cell proliferation, leading to pollination failure and male sterility.
Article
Biotechnology & Applied Microbiology
Yu Han, Sheng-Zhe Jiang, Xiang Zhong, Xing Chen, Chang-Kai Ma, Yan-Ming Yang, Yi-Chen Mao, Si-Da Zhou, Lei Zhou, Yan-Fei Zhang, Xue-Hui Huang, Hui Zhang, Lai-Geng Li, Jun Zhu, Zhong-Nan Yang
Summary: We reported a novel rice thermosensitive genic male sterility (TGMS) line ostms15, which is male sterile under high temperature and fertile under low temperature. Field evaluation from 2018 to 2021 revealed that ostms15 has more stable sterility under high temperature compared to tms5 (ZH11), indicating its considerable value for rice breeding. We also identified a point mutation in the OsTMS15 gene of ostms15, leading to the TGMS phenotype.
PLANT BIOTECHNOLOGY JOURNAL
(2023)