Article
Plant Sciences
Maryam Nasr Esfahani, Komaki Inoue, Kien Huu Nguyen, Ha Duc Chu, Yasuko Watanabe, Asaka Kanatani, David J. Burritt, Keiichi Mochida, Lam-Son Phan Tran
Summary: The study compared the transcriptome changes in chickpea roots and leaves under -Pi/+NO3-, +Pi/-NO3-, and -Pi/-NO3- conditions. The results showed that -Pi/-NO3- treatment had a lesser effect on gene expression changes related to Pi and NO3- transport, signalling networks, lipid remodelling, nitrogen and Pi scavenging/remobilization/recycling, carbon metabolism, and hormone metabolism compared to -Pi/+NO3- or +Pi/-NO3- treatments. Nutrient imbalance was found to be a stronger stimulus for molecular reprogramming than overall deficiency.
PLANT CELL AND ENVIRONMENT
(2021)
Article
Plant Sciences
Maryam Nasr Esfahani, Miyako Kusano, Mostafa Abdelrahman, Kien Huu Nguyen, Yasuko Watanabe, Keiichi Mochida, David J. Burritt, Lam-Son Phan Tran
Summary: This study aimed to identify key NO3- and/or Pi starvation-responsive metabolic pathways in chickpea and found significant differences in metabolic adaptation under single and double nutrient deficiencies. The simultaneous deficiency of NO3- and Pi generated unique metabolic changes that couldn't simply be explained by the combined deficiencies of the two nutrients.
Article
Plant Sciences
E. Matthus, K. A. Wilkins, A. Mohammad-Sidik, Y. Ning, J. M. Davies
Summary: Extracellular ATP increases cytosolic free calcium as a specific second messenger 'signature' through the DORN1/P2K1 receptor. The biphasic signature in Arabidopsis roots is affected by phosphate deprivation and is associated with wave formation. The spatial origin of the signature was resolved using bioluminescent aequorin and intensiometric GCaMP3 reporters. The study suggests that the root apex generates the first calcium increase, while the sub-apical region in the root hair zone has some autonomy and is reduced in Pi-starved roots.
Article
Plant Sciences
Patricia Ramalho de Barros, Samuel Vasconcelos Valadares, Nairam Felix de Barros, Hellen Oliveira de Oliveira, Wagner L. Araujo, Alice Pita-Barbosa
Summary: This study investigated the impacts of water limitation and calcium supply on the expression of calcium transporters, calcium acquisition, allocation, and its effects in eucalypt responses to drought. The results showed that water limitation increased the expression of ACA11 gene and decreased the expression of MCA1. Calcium supply alleviated the negative effects of water limitation on plant water status, photosynthesis, stomatal conductance, and transpiration. Water limitation altered the expression of calcium transporters in eucalypt roots, possibly contributing to an increase in calcium storage within root cells and a decrease in its influx into the endodermis.
JOURNAL OF SOIL SCIENCE AND PLANT NUTRITION
(2023)
Article
Agronomy
Alberto Marco Del Pino, Beatrice Falcinelli, Roberto D'Amato, Daniela Businelli, Paolo Benincasa, Carlo Alberto Palmerini
Summary: The biological activity of emmer wheatgrass extracts sprouted with distilled water, salinity, or selenium was tested on maize pollen grains, affecting both cytosolic Ca2+ and germination. Pure phenolic acids in unconjugated forms present in emmer wheatgrass were found to contribute to the biological activity of the extracts. Maize pollen treated with Se-enriched wheatgrass extracts showed less disruption in cytosolic Ca2+ and higher germination rate.
Article
Plant Sciences
Vitor Batista Pinto, Vinicius Costa Almeida, Italo A. Pereira-Lima, Ellen Moura Vale, Wagner L. Araujo, Vanildo Silveira, Jose Marcelo Soriano Viana
Summary: Al responsive proteins play a key role in carbohydrate metabolic pathways. Sucrose synthase may contribute to Al tolerance. Responses to Aluminum toxicity are regulated at transcriptional and post-transcriptional levels.
Article
Plant Sciences
Shuhan Yu, Jiaxuan Wu, Yanmei Sun, Haifeng Zhu, Qiguo Sun, Pengcheng Zhao, Risheng Huang, Zhenfei Guo
Summary: A calmodulin-like protein, MsCML10, in alfalfa activates glutathione S-transferase and fructose 1,6-biphosphate aldolase to improve cold tolerance by regulating ROS homeostasis and sugar accumulation.
Article
Agriculture, Multidisciplinary
Gurkan Demirkol
Summary: The study found that certain red clover populations showed significant down-regulation (miRNA156, miRNA171, and miRNA2111) and up-regulation (miRNA399) of miRNAs in response to long-term phosphate stress, indicating potential tolerance mechanisms. This suggests that these miRNAs could play a role in developing red clover plants tolerant to phosphate-deficient soils.
NEW ZEALAND JOURNAL OF AGRICULTURAL RESEARCH
(2022)
Article
Plant Sciences
Cristina Pignocchi, Alexander Ivakov, Regina Feil, Martin Trick, Marilyn Pike, Trevor L. Wang, John E. Lunn, Alison M. Smith
Summary: It is found that plant roots rely on sucrose imported from leaves for metabolism and growth. Reduced activity of cytosolic invertase leads to metabolic, growth, and developmental defects in Arabidopsis seedling roots. This indicates the importance of sugar signaling for growth and development in plants.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Biochemistry & Molecular Biology
Amirah Mohammad-Sidik, Jian Sun, Ryoung Shin, Zhizhong Song, Youzheng Ning, Elsa Matthus, Katie A. Wilkins, Julia M. Davies
Summary: The study identified Arabidopsis thaliana DORN1 as the first plant eATP receptor, fundamental in increasing cytosolic free Ca2+ levels and causing downstream responses like ROS and gene expression changes. The role of Annexin 1 in mediating ROS-activated Ca2+ influx in roots was confirmed, showing its involvement in eATP-induced responses on both Ca2+ levels and gene expression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agricultural Engineering
Viviane Cacefo, Alessandra Ferreira Ribas, Kezia Aparecida Guidorizi, Luiz Gonzaga Esteves Vieira
Summary: This study evaluated the effects of exogenous proline application on the ionomic profile of tobacco plants and found that proline application significantly affected the ionomic profile, rather than the water regime. The absorption and translocation mechanisms were likely affected by the higher amount of proline, leading to the separation of mineral elements into different groups. Ultimately, the data suggests that proline may act as a mitigator of nutritional deficiencies caused by water deficit conditions in tobacco plants.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Editorial Material
Plant Sciences
Uwe Ludewig, Emil Vatov, Dominik Hedderich, Benjamin Neuhaeuser
Summary: The NIGT1/HHO-type transcriptional repressors activate phosphate deprivation responses by inhibiting upstream repressors and suppress nitrate deprivation responses when nitrate is provided. Their loss leads to increased nitrate uptake and plant growth. Safi et al. (2021) connect the function of NIGT1/HHOs with reactive oxygen species (ROS) signaling under fluctuating nitrate conditions, but the integration of ROS into nutrient-specific signaling cascades remains unclear.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Microbiology
Helena Sophia Domes, Enzo Neu, Marcus Linde, Thomas Debener
Summary: Phosphorous starvation affects the interaction between roses and black spot pathogens, as well as the gene expression, which is genotype-dependent.
Article
Biochemistry & Molecular Biology
Ricardo Ortiz-Luevano, Jose Lopez-Bucio, Miguel Martinez-Trujillo, Lenin Sanchez-Calderon
Summary: Plants, as sessile organisms, adjust their genetic expression, metabolism, and developmental program in response to changing environments. Lead (Pb) is a hazardous pollutant that can limit crop productivity. Research shows that Pb has a biphasic dose response effect on biomass accumulation in plants, affecting root system architecture and inducing medium acidification. Additionally, Pb can regulate physiological responses in the root system, interacting with phosphate (Pi) starvation response pathways.
Article
Plant Sciences
So-Eun Kim, Xiaofeng Bian, Chan-Ju Lee, Sul-U Park, Ye-Hoon Lim, Beg Hab Kim, Woo Sung Park, Mi-Jeong Ahn, Chang Yoon Ji, Yang Yu, Yizhi Xie, Sang-Soo Kwak, Ho Soo Kim
Summary: Transgenic sweetpotato plants overexpressing IbHPPD exhibited enhanced tolerance to various environmental stresses, including salt, drought, and oxidative stresses. They also showed increased resistance to the herbicide sulcotrione, which inhibits HPPD. Additionally, these plants had higher levels of alpha-tocopherol content under dehydrated conditions.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Plant Sciences
Ren Wang, Ellie Himschoot, Matteo Grenzi, Jian Chen, Alaeddine Safi, Melanie Krebs, Karin Schumacher, Moritz K. Nowack, Wolfgang Moeder, Keiko Yoshioka, Daniel Van Damme, Ive De Smet, Danny Geelen, Tom Beeckman, Jiri Friml, Alex Costa, Steffen Vanneste
Summary: This study evaluates the effects of different auxin analogs on the accumulation of PIN proteins in BFA bodies and their ability to induce Ca2+ responses. The results show that not all auxin analogs can elicit Ca2+ responses and their ability to do so partially correlates with their inhibition of BFA body formation.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Plant Sciences
Shahin Imran, Masumi Oyama, Rie Horie, Natsuko Kobayashi, Alex Costa, Ryosuke Kumano, Chiho Hirata, Sen Thi Huong Tran, Maki Katsuhara, Keitaro Tanoi, Takayuki Kohchi, Kimitsune Ishizaki, Tomoaki Horie
Summary: This study investigates the structure and function of the high-affinity potassium transporter (MpHKT1) in the liverwort Marchantia polymorpha. The C-terminal hydrophilic domain of MpHKT1 is found to play a unique role in regulating transport activity and ion selectivity. Overexpression of MpHKT1 leads to higher Na+ accumulation, while phenotypes with truncated C-terminal domains are weaker.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Plant Sciences
E. Matthus, K. A. Wilkins, A. Mohammad-Sidik, Y. Ning, J. M. Davies
Summary: Extracellular ATP increases cytosolic free calcium as a specific second messenger 'signature' through the DORN1/P2K1 receptor. The biphasic signature in Arabidopsis roots is affected by phosphate deprivation and is associated with wave formation. The spatial origin of the signature was resolved using bioluminescent aequorin and intensiometric GCaMP3 reporters. The study suggests that the root apex generates the first calcium increase, while the sub-apical region in the root hair zone has some autonomy and is reduced in Pi-starved roots.
Article
Chemistry, Multidisciplinary
Kanane Sato, Shunya Saito, Kohsuke Endo, Masaru Kono, Taishin Kakei, Haruka Taketa, Megumi Kato, Shin Hamamoto, Matteo Grenzi, Alex Costa, Shintaro Munemasa, Yoshiyuki Murata, Yasuhiro Ishimaru, Nobuyuki Uozumi
Summary: This study identifies components of green tea, (-)-catechin gallate (CG) and (-)-gallocatechin gallate (GCG), as inhibitors of voltage-dependent K+ channels in Arabidopsis thaliana guard cells. The findings reveal the crucial role of plasma membrane I-Ca channels in ABA-induced stomatal closure and provide new tools for the study of stress-induced signal transduction pathways.
Article
Cell Biology
Margherita Festa, Velia Minicozzi, Anna Boccaccio, Laura Lagostena, Antonella Gradogna, Tianwen Qi, Alex Costa, Nina Larisch, Shin Hamamoto, Emanuela Pedrazzini, Stefan Milenkovic, Joachim Scholz-Starke, Matteo Ceccarelli, Alessandro Vitale, Petra Dietrich, Nobuyuki Uozumi, Franco Gambale, Armando Carpaneto
Summary: This review focuses on current methods used to unravel the functional properties of lysosomal ion channels and transporters, evaluating their advantages, disadvantages, and fields of applicability.
Review
Plant Sciences
Matteo Grenzi, Maria Cristina Bonza, Alex Costa
Summary: Plant glutamate receptor-like channels (GLRs) play important roles in signal transduction processes in plants, particularly in regulating ion fluxes, especially calcium fluxes. They are involved in various signaling processes related to pollen development, sexual reproduction, root development, stomatal regulation, and response to pathogens.
CURRENT OPINION IN PLANT BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Cristina Ruberti, Elias Feitosa-Araujo, Zhaolong Xu, Stephan Wagner, Matteo Grenzi, Essam Darwish, Sophie Lichtenauer, Philippe Fuchs, Ambra Selene Parmagnani, Daria Balcerowicz, Sebastjen Schoenaers, Carolina de la Torre, Khansa Mekkaoui, Adriano Nunes-Nesi, Markus Wirtz, Kris Vissenberg, Olivier Van Aken, Bettina Hause, Alex Costa, Markus Schwarzlaender
Summary: The study demonstrates that MCU proteins mediate mitochondrial Ca2+ transport in plants, serving as the major route for fast Ca2+ uptake. Knockout of mcu genes limits mitochondrial Ca2+ uptake, leading to impaired subcellular Ca2+ transport and deregulated jasmonic acid-related signaling and thigmomorphogenesis.
Article
Plant Sciences
Rosario Pantaleno, Denise Scuffi, Alex Costa, Elina Welchen, Roberta Torregrossa, Matthew Whiteman, Carlos Garcia-Mata
Summary: Hydrogen sulfide (H2S) is a gaseous signaling molecule that plays a role in physiological processes in plants, including regulating stomatal pore width. In this study, it was found that a mitochondrial-targeted H2S donor called AP39 can induce stomatal closure in a dose-dependent manner. The activity of mitochondrial cytochrome c (CYTc) and/or complex IV was shown to be necessary for AP39-dependent stomatal closure. AP39 also affected the mitochondrial inner potential, cytosolic ATP, hydrogen peroxide levels, and the oxidation of the glutathione pool in guard cells. These findings highlight the interplay between mitochondrial H2S, mitochondrial activity, and stomatal closure.
Article
Biochemistry & Molecular Biology
Matteo Grenzi, Stefano Buratti, Ambra Selene Parmagnani, Ilaria Abdel Aziz, Iwona Bernacka-Wojcik, Francesca Resentini, Jan Simura, Fabrizio Gandolfo Doccula, Andrea Alfieri, Laura Luoni, Karin Ljung, Maria Cristina Bonza, Eleni Stavrinidou, Alex Costa
Summary: In Arabidopsis thaliana, local wounding and herbivore feeding trigger Ca2+ waves that are dependent on the activity of glutamate receptor-like channels (GLRs). GLRs are also required for the synthesis of jasmonic acid (JA) and the subsequent signaling response for plant acclimation. The mechanism of GLR activation is still unclear.
Article
Plant Sciences
Nick S. Fradgley, Alison R. Bentley, Keith A. Gardner, Stephanie M. Swarbreck, Matt Kerton
Summary: Improved selection of wheat varieties with high end-use quality has been achieved through genetic control and genomic prediction analysis of historic and elite UK bread wheat varieties. The study found that genetic diversity has not declined over recent decades of selective breeding, and the loaf baking quality of modern milling wheats has increased. Genome-wide association analysis identified multiple quantitative trait loci (QTL) with pleiotropic effects on quality traits. Marker-assisted selection has the potential to improve specific weight and Hagberg falling number traits. Applying genomic prediction in wheat breeding programs outperforms phenotypic selection, and trait-assisted genomic prediction improves prediction accuracy. Genomic prediction alone is the most cost-effective selection strategy.
Review
Plant Sciences
Nicholas H. Doddrell, Tracy Lawson, Christine A. Raines, Carol Wagstaff, Andrew J. Simkin
Summary: Several long-term studies have shown that growing crops under elevated CO2 can increase photosynthesis and lead to higher yield, flavor, and nutritional content. For example, tomatoes grown at 1000 ppm CO2 have shown yield increases of up to 80%, which is consistent with industry practices. This review focuses on evaluating the impact of elevated CO2 on fruit yield and nutritional quality over 50 years, and suggests the need for engineering improvements to optimize plant growth under high CO2 conditions.
HORTICULTURE RESEARCH
(2023)
Article
Genetics & Heredity
Lisa Pavinato, Jennifer Stanic, Marta Barzasi, Antonia Gurgone, Giuseppe Chiantia, Valentina Cipriani, Ivano Eberini, Luca Palazzolo, Monica Di Luca, Alex Costa, Andrea Marcantoni, Elisa Biamino, Marco Spada, Susan M. Hiatt, Whitley Kelley, Letizia Vestito, Stephanie Efthymiou, Prem Chand, Rauan Kaiyrzhanov, Alessandro Bruselles, Simona Cardaropoli, Marco Tartaglia, Silvia De Rubies, Joseph D. Buxbaum, Damian Smedley, Giovanni Battista Ferrero, Maurizio Giustetto, Fabrizio Gardoni, Alfredo Brusco, Genomics England Res Consortium
Summary: This study demonstrates that missense gain-of-function variants in RPH3A increase the levels of GluN2A-containing NMDA ionotropic glutamate receptors at extrasynaptic sites, altering synaptic function and leading to clinically variable neurodevelopmental disorders ranging from untreatable epilepsy to autism spectrum disorder.
GENETICS IN MEDICINE
(2023)
Article
Plant Sciences
Matteo Pivato, Matteo Grenzi, Alex Costa, Matteo Ballottari
Summary: The role of intracellular Ca2+ signaling in the perception and response mechanisms to light in unicellular microalgae was investigated using the Yellow Cameleon (YC3.6) Ca2+ indicator expressed in Chlamydomonas reinhardtii. Light-induced Ca2+ signaling was observed in the chloroplast, influenced by light intensity and photosynthetic electron transport. The absence of the photoreceptors aCRY and PHOT had different effects on the chloroplast Ca2+ response, while a correlation between H2O2 gradients and Ca2+ transients was observed.
Article
Plant Sciences
Yinwei Zeng, Inge Verstraeten, Hoang Khai Trinh, Robin Lardon, Sebastien Schotte, Damilola Olatunji, Thomas Heugebaert, Christian Stevens, Mussa Quareshy, Richard Napier, Sara Paola Nastasi, Alex Costa, Bert De Rybel, Catherine Bellini, Tom Beeckman, Steffen Vanneste, Danny Geelen
Summary: This study reveals the molecular processes involved in the initiation of adventitious root (AR) formation along the hypocotyl of Arabidopsis seedlings exposed to light. The results show that AR induction by HYSPARIN involves nuclear TIR1/AFB and plasma membrane TMK auxin signaling, as well as multiple downstream LR development genes. Additionally, SAUR19, OFP4, and AGC2 are identified as novel regulators of AR formation.
Article
Multidisciplinary Sciences
Adam Armada-Moreira, Abdul Manan Dar, Zifang Zhao, Claudia Cea, Jennifer Gelinas, Magnus Berggren, Alex Costa, Dion Khodagholy, Eleni Stavrinidou
Summary: We developed a conformable multielectrode array based on organic electronics and used it to study the electrical signals in plants. By mapping the action potential in Venus flytrap, we discovered its propagation characteristics and its correlation with trap movement. We also found that cells other than sensory hairs can activate the Venus flytrap circuitry. This work contributes to the understanding of electrical signaling in plants and its role in long-distance responses.