Article
Chemistry, Analytical
Shashini De Silva, Iran Ocana-Rios, Cecilia Cagliero, Morgan R. Gostel, Gabriel Johnson, Jared L. Anderson
Summary: A simple and rapid ionic liquid-based extraction method was developed for isolating genomic DNA from Arabidopsis thaliana plant tissue. The method involved using ionic liquid or magnetic ionic liquid directly applied to treated plant tissue to facilitate cell lysis and DNA extraction. The DNA-enriched extracts could be directly used in qPCR without needing additional purification steps and showed good preservation of plant DNA at room temperature.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biochemistry & Molecular Biology
Jorge David Cadena-Zamudio, Juan Luis Monribot-Villanueva, Claudia-Anahi Perez-Torres, Fulgencio Alatorre-Cobos, Jose Antonio Guerrero-Analco, Enrique Ibarra-Laclette
Summary: This study analyzed the metabolic plasticity of Arabidopsis thaliana in response to nitrate deprivation and found that the metabolic responses in shoots and roots contribute to growth or stress resistance. For normally growing Arabidopsis, the metabolic responses favor the synthesis of growth-related metabolites, while under nitrate deprivation, the metabolic responses prioritize defensive reactions over growth.
Article
Chemistry, Multidisciplinary
Z. Elyasi, G. R. Najafi, J. Safaei Ghomi, M. A. Sharif
Summary: This study introduces a novel polymerized ionic liquid as a catalyst for the efficient synthesis of pharmaceutical scaffolds with broad structural diversity under environmentally-friendly conditions.
MATERIALS TODAY CHEMISTRY
(2022)
Article
Biochemical Research Methods
Miranda N. Emaus, Cecilia Cagliero, Morgan R. Gostel, Gabriel Johnson, Jared L. Anderson
Summary: In this study, a fast and convenient method for plant DNA sample preparation using hydrophobic magnetic ionic liquids and ionic liquids was developed. This method does not require high temperatures or chemical surfactants and can be completed in one minute or less, making it suitable for fieldwork and lab work in low resource environments.
Article
Plant Sciences
V. N. Popov, A. N. Deryabin
Summary: The effect of photoperiod duration on the efficiency of low-temperature hardening was investigated in Arabidopsis thaliana plants. It was found that plants exposed to cold acclimation in the dark did not show frost resistance, while plants hardened in the light improved their frost resistance. A longer photoperiod of 16 hours resulted in higher levels of sugar accumulation and faster development of frost resistance compared to an 8-hour photoperiod. Therefore, a combination of low temperature and a 16-hour photoperiod is required for rapid formation of frost resistance in Arabidopsis thaliana.
RUSSIAN JOURNAL OF PLANT PHYSIOLOGY
(2023)
Article
Chemistry, Inorganic & Nuclear
Andreia Forte, Sandra Gago, Manuela Ribeiro Carrott, Peter Carrott, Celso Alves, Fernando Teodoro, Rui Pedrosa, Isabel M. Marrucho, Luis C. Branco
Summary: Organic salts based on different choline derivative cations and metal ions were immobilized in mesoporous silica nanoparticles through a two-step synthetic method, characterized using various techniques and evaluated for cytotoxicity in different cell lines. The characterization data confirmed successful functionalization and provided insights into the materials' properties, while the cytotoxicity results revealed differences between the metal organic salts and their immobilized analogues.
DALTON TRANSACTIONS
(2021)
Article
Plant Sciences
V. N. Popov, N. V. Astakhova
Summary: Changes in invertase activities were studied in chilling-sensitive tobacco and cold-resistant Arabidopsis plants upon hardening to hypothermia. In tobacco, cytoplasmic and vacuolar invertase activities decreased while cell wall invertase activity increased. On the other hand, all three types of invertases in arabidopsis showed an increase in activity. This increase in invertase activity led to enhanced sugar accumulation in both plant species, with different effects on sucrose and hexose proportions.
RUSSIAN JOURNAL OF PLANT PHYSIOLOGY
(2021)
Article
Chemistry, Physical
Illia V. Kapitanov, Grete Raba, Marcel Spulak, Raivo Vilu, Yevgen Karpichev, Nicholas Gathergood
Summary: A series of dipeptide ionic liquids (ILs) containing L-phenylalanine and L-alanine fragments were synthesized and their possible degradation pathways were analyzed. The compounds were screened for microbial toxicity and aerobic biodegradation, and the results showed that L-phenylalanine derivatives had higher biodegradability compared to L-alanine derivatives.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Chemistry, Physical
Mu Naushad, Tansir Ahamad, Mohammad Rizwan Khan
Summary: In this study, novel magnetic ionic liquid based nanocomposites were fabricated for the efficient production of biodiesel from palm oil. The results showed that [NiFe2O4@BMSI]HSO4 exhibited higher catalytic performance and reusability compared to NiFe2O4@BMSI]Br, making it a promising catalyst for transesterification reactions.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Biochemistry & Molecular Biology
Siddhant Chaturvedi, Neha Thakur, Shahirina Khan, Mithilesh Kumar Sardar, Alka Jangra, Siddharth Tiwari
Summary: In this study, the banana cultivar Nendran was found to have the highest concentration of ascorbic acid (AsA). Five genes related to AsA synthesis were identified from the banana genome database, and three of them were shown to significantly enhance AsA levels in plant leaves through overexpression in Arabidopsis thaliana. The findings of this study have important implications for the development of AsA-rich plants, particularly in developing countries where staple foods are crucial.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Plant Sciences
Fahimeh Koohdar, Masoud Sheidai
Summary: Medicinal plants are crucial for disease treatment in many countries, however, fraud and misconduct in sales centers can pose a threat to customer health. This study found that using internal transcribed spacer and chloroplast DNA sequences can effectively differentiate Melissa officinalis from other species.
Article
Chemistry, Physical
Nabeel Mujtaba Abbasi, Victoria R. Zeger, Anis Biswas, Jared L. Anderson
Summary: This study focuses on magnetic ionic liquids (MILs) containing paramagnetic centers. It shows that modifications in the chemical structure of diglycolamides can influence the physico-chemical properties of MILs. Transition metals can improve thermal stability and reduce viscosity, leading to higher effective magnetic moments. The results highlight the importance of structural tunability in MILs for various applications such as organic synthesis and chemical separations.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Biochemistry & Molecular Biology
Liwen Xiong, Zirong Li, Weihua Li, Lanzhi Li
Summary: In this study, a new method called DT-PICS was developed for efficient and cost-effective SNP selection in germplasm identification. The method uses a decision tree concept to select the most informative SNPs and reduces redundancy in the selection process. The results demonstrate that DT-PICS is effective in germplasm identification and management, providing support for future plant breeding and conservation efforts.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Chemical
Ana M. Ferreira, Maria V. Quental, Mukesh Sharma, Dibyendu Mondal, Augusto Q. Pedro, Joao A. P. Coutinho, Mara G. Freire
Summary: Early infection diagnosis is essential for reducing morbidity and mortality rates. A study investigated the use of aqueous biphasic systems (ABS) composed of polypropylene glycol 400 and cholinium-based ionic liquids (ILs) at different pH values for pre-treating human serum, aiming to separate DNA from human serum albumin (HSA) to improve the accuracy of DNA quantification. The ABS demonstrated high extraction efficiencies of DNA to the IL-rich phase, with minimal DNA loss. It was found that adjusting the pH allowed for selective isolation of DNA from HSA. The IL-rich phase of the ABS was shown to maintain DNA integrity and could potentially serve as a DNA storage medium.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Zhongfang Xing, Ruiqian Zhang, Zhehui Zhao, Liangliang Wang, Ling Yuan, Huilan Yu, Yang Yang, Yuntao Yang, Shilei Liu, Chengxin Pei
Summary: In this study, the model plant Arabidopsis thaliana (L.) was exposed to the nerve agent iBuVX. Through analysis, new phosphonyl-modified flavonoid compounds were identified as potential biomarkers, which has significant implications for the retrospective analysis of organophosphorus exposure for chemical weapon verification and forensic analysis.
Review
Chemistry, Analytical
Cecilia Cagliero, Carlo Bicchi, Arianna Marengo, Patrizia Rubiolo, Barbara Sgorbini
Summary: This review provides an overview of recent advances in gas chromatography for essential oil analysis. It focuses on new stationary phases, advanced analytical methods, and instruments. The review explores the use of ionic liquids as stationary phases and strategies to speed up analysis time. It also discusses recent developments in column miniaturization and includes applications in the field of essential oils.
JOURNAL OF SEPARATION SCIENCE
(2022)
Review
Chemistry, Applied
Giulia Strocchi, Patrizia Rubiolo, Chiara Cordero, Carlo Bicchi, Erica Liberto
Summary: This review critically analyzes the accumulated knowledge on the formation of AA in coffee and the mitigation strategies that have been investigated. It focuses on current applicability in the industry and little explored topics.
Article
Biochemistry & Molecular Biology
Giuseppe Mannino, Maddalena Ricciardi, Noemi Gatti, Graziella Serio, Ivano Vigliante, Valeria Contartese, Carla Gentile, Cinzia M. Bertea
Summary: The application of a biostimulant based on algae and yeast extracts showed significant effects on the peach trees, reducing ripening time, increasing fruit size, and boosting the content of bioactive compounds. This study highlights the importance of plant biostimulants in sustainable agriculture.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Analytical
Shashini De Silva, Iran Ocana-Rios, Cecilia Cagliero, Morgan R. Gostel, Gabriel Johnson, Jared L. Anderson
Summary: A simple and rapid ionic liquid-based extraction method was developed for isolating genomic DNA from Arabidopsis thaliana plant tissue. The method involved using ionic liquid or magnetic ionic liquid directly applied to treated plant tissue to facilitate cell lysis and DNA extraction. The DNA-enriched extracts could be directly used in qPCR without needing additional purification steps and showed good preservation of plant DNA at room temperature.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Multidisciplinary
Giulia Mastellone, Nabeel Mujtaba Abbasi, Cecilia Cagliero, Jared L. Anderson
Summary: Deep eutectic solvents (DESs) are sustainable solvents that can easily modify their properties by changing the type of hydrogen bond donor (HBD) and hydrogen bond acceptor (HBA). This study synthesized hydrophobic [Ch+][Br-]-modified salts for the preparation of hydrophobic DESs. The [Ch+][Br-]-based HDESs were characterized and used as solvents for microextraction of phytochemicals in Cannabis sativa L. plant, showing good extraction performance for both hydrophilic and hydrophobic compounds.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2023)
Article
Biochemistry & Molecular Biology
Gaia Bechis, Manuel A. Minteguiaga, Barbara Sgorbini, Arianna Marengo, Patrizia Rubiolo, Cecilia Cagliero
Summary: In this study, enantioselective gas chromatography (GC) was used to evaluate the quality of sweet and bitter orange essential oils. The use of fast GC significantly reduced the environmental footprint of the analyses. The results showed that fast chiral gas chromatography is an environmentally friendly and sustainable approach for evaluating the quality of citrus essential oils.
Article
Plant Sciences
Marta Pavarino, Arianna Marengo, Cecilia Cagliero, Carlo Bicchi, Patrizia Rubiolo, Barbara Sgorbini
Summary: The essential oil of E. cardamomum inhibits AChE and BChE enzymes, and the activity is attributed to a pool of oxygenated terpenes.
Article
Chemistry, Multidisciplinary
Gaia Bechis, Bastien Raccary, Elise Sarrazin, Elise Corbi, Christophe Peres, Nathalie David, Carlo Bicchi, Cecilia Cagliero
Summary: Enantioselective gas chromatography is used in fragrance quality control laboratories to authenticate raw materials and detect fraud or adulteration of perfumes. This study developed a GC-MS method for chiral recognition of odorants and evaluated the environmental improvement of fast GC using AGREE. A multi-criteria approach based on the RGB model was developed, accurately accounting for energy and solvent consumption while maintaining analytical performance and laboratory productivity. The assessment showed that fast GC improves the greenness and productivity of a laboratory, lowering costs while maintaining adequate analytical performance, making it a sustainable approach.
SUSTAINABLE CHEMISTRY AND PHARMACY
(2023)
Article
Plant Sciences
Arianna Marengo, Larissa Silva Maciel, Cecilia Cagliero, Patrizia Rubiolo, Koit Herodes
Summary: This study aims to obtain, for the first time, the amino compounds profile of Cardueae plants using statistical analysis to detect similarities and differences among different samples. The results show that the DEEMM-derivatized amino compounds are qualitatively similar among the samples but can be discriminated at the genus level. This work provides more information on the phytochemical composition and potential health benefits of these little-studied plant species.