期刊
PLANT CELL
卷 31, 期 3, 页码 579-601出版社
AMER SOC PLANT BIOLOGISTS
DOI: 10.1105/tpc.18.00742
关键词
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资金
- U.S. Department of Energy, Office of Science, Office of Biological and Environmental Research [DE-SC0018301]
- U.S. Department of Agriculture National Institute of Food and Agriculture [2013-67012-21272]
- U.S. Department of Energy Joint Genome Institute, a Department of Energy Office of Science User Facility [DE-AC02-05CH11231]
- U.S. Department of Energy, Office of Science, through the Photosynthetic Systems program in the Office of Basic Energy Sciences
- National Institute of General Medical Sciences of the National Institutes of Health [P41GM103445]
- U.S. Department of Energy, Office of Biological and Environmental Research [DE-AC02-05CH11231]
- National Institutes of Health S10 program [1S10OD018136-01]
- Department of Energy Joint BioEnergy Institute, Office of Biological and Environmental Research [DE-AC02-05CH11231]
- National Institutes of Health National Institute of General Medical Sciences [P01GM051487]
- National Science Foundation Graduate Research Fellowship
- U.S. Department of Energy (DOE) [DE-SC0018301] Funding Source: U.S. Department of Energy (DOE)
Light and nutrients are critical regulators of photosynthesis and metabolism in plants and algae. Many algae have the metabolic flexibility to grow photoautotrophically, heterotrophically, or mixotrophically. Here, we describe reversible Glc-dependent repression/activation of oxygenic photosynthesis in the unicellular green alga Chromochloris zofingiensis. We observed rapid and reversible changes in photosynthesis, in the photosynthetic apparatus, in thylakoid ultrastructure, and in energy stores including lipids and starch. Following Glc addition in the light, C. zofingiensis shuts off photosynthesis within days and accumulates large amounts of commercially relevant bioproducts, including triacylglycerols and the high-value nutraceutical ketocarotenoid astaxanthin, while increasing culture biomass. RNA sequencing reveals reversible changes in the transcriptome that form the basis of this metabolic regulation. Functional enrichment analyses show that Glc represses photosynthetic pathways while ketocarotenoid biosynthesis and heterotrophic carbon metabolism are upregulated. Because sugars play fundamental regulatory roles in gene expression, physiology, metabolism, and growth in both plants and animals, we have developed a simple algal model system to investigate conserved eukaryotic sugar responses as well as mechanisms of thylakoid breakdown and biogenesis in chloroplasts. Understanding regulation of photosynthesis and metabolism in algae could enable bioengineering to reroute metabolism toward beneficial bioproducts for energy, food, pharmaceuticals, and human health.
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