Article
Chemistry, Analytical
Yi Gu, Yang Guo, Yang Deng, Haipeng Song, Rui Nian, Wenshuai Liu
Summary: A novel pentameric nanobodies-based immunoassay (PNIA) demonstrated superior sensitivity and high specificity for detecting CEACAM-5 antigen. The use of bispecific or multivalent nanobody design provided new insights into the development of immunoassays for clinical diagnosis.
ANALYTICA CHIMICA ACTA
(2023)
Article
Food Science & Technology
Guifang Xie, Ying Lu, Wenkai Li, Zhenyun He, Zhichang Sun, Xiaoxia Xie, Xing Liu
Summary: Antibody affinity and enzyme loads are critical factors in determining the sensitivity of enzyme-linked immunosorbent assay for small molecular contaminants in food. This study successfully improved the antibody affinity and enzyme loads by utilizing the strategy of multimerization, and developed an enhanced colorimetric enzyme immunoassay for ochratoxin A (OTA).
Article
Chemistry, Multidisciplinary
Jiahao Chen, Yingtian Xu, Yang Liu, Heng Liu, He Zhang, Liang Jin, Linlin Shi, Yunping Lan, Yonggang Zou, Jie Fan
Summary: This study successfully synthesized a 2D conjugated metal-organic framework and graphene stacked vertical heterostructure. Theoretical simulations and experimental characterizations showed that the charge transfer rate and nonlinear optical properties of this structure were significantly enhanced, demonstrating its potential in the field of ultrafast photonics.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Chemistry, Analytical
Yun Chen, Wei Duan, Li Xu, Guanghui Li, Yakun Wan, Henan Li
Summary: In this study, a nanobody-based immunosensor was developed for rapid detection of SARS-CoV-2 spike protein. The immunosensor showed high sensitivity and efficiency, providing a new convenient and rapid method for SARS-CoV-2 detection.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Applied
Wenting Zou, Ruirui Shi, Guanqun Wang, Zhiling Zhao, Fengchun Zhao, Zhengyou Yang
Summary: In this study, a noncompetitive immunoassay for detecting AFB1 was developed using phage-display technology. Compared with competitive immunoassays, this method exhibited higher sensitivity and efficiency, and achieved satisfactory recovery rates in various sample matrices.
Article
Engineering, Environmental
Shaowen Wu, Jintao Xu, Wenxing Chen, Fenghua Wang, Xiaoliang Tan, Xinlu Zou, Weijie Zhou, Wenjie Huang, Yixiong Zheng, Shihua Wang, Shijuan Yan
Summary: This study presents a Scaffold Assembly-based Bioluminescent Enzyme Immunoassay that overcomes the limitations of traditional methods and achieves highly sensitive detection of mycotoxins. The programmable conjugation of nanobodies and luciferases onto protein nanoscaffolds allows for optimized nanobody/luciferase ratios and diversified specificities. Compared to conventional techniques, this method demonstrates elevated sensitivity and cost-effectiveness, and enables the simultaneous detection of multiple mycotoxins.
JOURNAL OF HAZARDOUS MATERIALS
(2024)
Article
Chemistry, Applied
Wen-jia Yin, Jing-xian Zhang, Hong Wang, Yu Wang, Xi Zeng, Zhen-lin Xu, Jin-yi Yang, Zhi-li Xiao, Bruce D. Hammock, Peng Wen
Summary: A sensitive and reliable electrochemical immunosensor based on cross-linked polyvinyl alcohol/citric acid nanofiber membrane (PVA/CA NFM) and horseradish peroxidase labeled anti-parathion nanobody was constructed for monitoring the residual of parathion pesticide in food. The immunosensor exhibited high specificity and sensitivity to parathion and showed good stability.
Article
Engineering, Environmental
Lin Luo, Shi-Qi Lin, Zhuo-Yu Wu, Hong Wang, Zi-Jian Chen, Hao Deng, Yu-Dong Shen, Wen-Feng Zhang, Hong-Tao Lei, Zhen-Lin Xu
Summary: A nanobody-alkaline phosphatase fusion protein-based fluorescent ELISA method was developed for highly sensitive detection of fenitrothion (FN) residue in food. The detection limit of this method was 0.14 ng mL-1, which is 25-fold lower than that of conventional colorimetric ELISAs.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Microbiology
Yaru Zhao, Jifei Yang, Qingli Niu, Jinming Wang, Mengyao Jing, Guiquan Guan, Meng Liu, Jianxun Luo, Hong Yin, Zhijie Liu
Summary: African swine fever is a deadly disease in domestic and wild swine, causing significant economic losses. This study successfully constructed a high-quality phage display library containing specific nanobodies against ASFV proteins. Two nanobodies, Nb17 and Nb30, were used to develop a sandwich ELISA for the detection of ASFV in clinical specimens. The newly developed assay showed high sensitivity and specificity, providing a valuable alternative technique for ASF detection and surveillance.
JOURNAL OF CLINICAL MICROBIOLOGY
(2023)
Article
Agriculture, Multidisciplinary
Yaozhong Hu, Jing Lin, Lijun Peng, Xuemeng Ji, Huan Lv, Jing Wu, Yan Zhang, Shuo Wang, Yi Wang, Sihao Wu
Summary: A nanobody-based micro-total electrochemical immunoassay (Nb-mu TEI) was developed for reliable and accurate detection of peanut allergens, with emphasis on Ara h 1. This study successfully identified the target Ara h 1 using Nb-mediated immunocapturing technique, after immunizing an alpaca and selecting four specific Nbs. The developed Nb-based electrochemical immunoassay exhibited an 11-fold increase in sensitivity compared with the traditional sandwich ELISA, with a linear range of 4.5-55 ng/mL and LOD and LOQ values of 0.86 and 2.10 ng/mL, respectively. The immunoassay was validated by detecting spiked antigens in food samples.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Chemistry, Analytical
Pengyan Guo, Kaiyin Huang, Zijian Chen, Zhenlin Xu, Aifen Ou, Qingchun Yin, Hong Wang, Xing Shen, Kai Zhou
Summary: The advantages of genetic modification and preferable physicochemical qualities make nanobody (Nb) easy to develop a sensitive and stable immunosensor platform. Herein, an indirect competitive chemiluminescence enzyme immunoassay (ic-CLEIA) based on biotinylated Nb was established for the quantification of diazinon (DAZ). The results showed that the proposed method based on Nb-biotin had a high specificity and sensitivity to DAZ, with a relative broader linear range and good recovery rates.
Article
Chemistry, Multidisciplinary
Huihua Zhang, Hui Qu, Jingbo Cui, Linxia Duan
Summary: A simple electrochemical immunosensor based on a chitosan/reduced graphene oxide (CS-rGO) nanocomposite was developed for sensitive determination of S-100B protein, a tumor marker of malignant melanoma. The immunosensor exhibited good stability, high selectivity and sensitivity, and can be applied for the sensitive detection of S-100B protein in real human serum samples.
Article
Chemistry, Physical
Xiaoli Liu, Hongxia Zhang, Junping Ma, Jianbin Zheng
Summary: A novel non-enzymatic nitrite (NO2-) electrochemical sensor based on Au-poly(thionine) and tin oxide/graphene nanosheets (Au-PTH/SGN) nanocomposites was successfully fabricated. The sensor exhibited a wide linear range and high sensitivity, and achieved satisfactory detection results in tap water and sewage samples.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2022)
Article
Chemistry, Applied
Ting Lv, Boxu Wang, Ningyi Xu, Baitong Shang, Ni Liu, Changshun Su, Chuanyu Yang, Hongxia Li, Zhenlin Xu, Chunyan Sun
Summary: In this study, a sensitive fluorescence immunoassay (FIA) was designed for detecting the residues and degradation of organophosphorus pesticides (OPs) in crops. The FIA utilized nanobody-linked alkaline phosphatase (Nb-ALP) and gold nanoclusters anchored manganese dioxide (AuNCs-MnO2) composite. The developed FIA exhibited high detection sensitivity and was successfully applied for monitoring the degradation of fenitrothion (FNT) in Chinese cabbage, paving the way for determining pesticide residues in real food samples.
Article
Chemistry, Analytical
Naruto Miyakawa, Ayumi Shinagawa, Tomomi Nakano, Shota Ushiba, Takao Ono, Yasushi Kanai, Shinsuke Tani, Masahiko Kimura, Kazuhiko Matsumoto
Summary: Graphene is a promising platform for sensing biomolecule charges. We proposed a graphene chemiresistor enzyme immunoassay that can detect all protein types with high sensitivity.
MICROCHEMICAL JOURNAL
(2024)
Article
Chemistry, Multidisciplinary
Kaiqing Wu, Ran Chen, Zhixin Zhou, Xinghua Chen, Yanqin Lv, Jin Ma, Yanfei Shen, Songqin Liu, Yuanjian Zhang
Summary: By using time-dependent electrochemiluminescence (Td-ECL) technique, we have successfully obtained the time and spatial distribution of reactive oxygen species (ROS) in the diffusion layer, and discovered multiple ultrasensitive stoichiometric reactions between ROS and the ECL emitter. This finding can guide the development of electrocatalysts for fuel cells and H2O2 production with maximized activity and durability.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biophysics
Mengyuan Chen, Zhenqiang Ning, Xue Ge, Erli Yang, Qian Sun, Fei Yin, Mingming Zhang, Yuanjian Zhang, Yanfei Shen
Summary: This study presents a simple and effective strategy to prepare gold nanoclusters with high photoluminescence (PL) through ligands engineering. The assembled arginine ligands on the MTU-protected Au nanoclusters led to significantly enhanced PL emission, which displayed rapid and reversible responses to various types of ions. These findings provide new insights into regulating the PL emission of Au nanoclusters and advance their potential applications in information encryption and bioassays.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Engineering, Electrical & Electronic
Xin Chen, Yaoyao Jin, Zipei Tao, Li Jiang, Xinjiang Wu, Yongyue Xiao, Bei Jiang, Xin Wen, Jun Zhang, Min Zhu, Cong Ye
Summary: As AI technology advances, memristors are proving to be effective artificial synapses that replicate various biological learning behaviors. By incorporating a 3-nm IGZO layer into the Pt/ZrO2/TiN memristor, the storage capacity of the bilayer memristor is significantly increased. It demonstrates the multilevel conductivity modulating properties of the bilayer memristors, which can mimic the learning behavior of biological synapses. Furthermore, the Pt/ZrO2/IGZO/TiN memristor has achieved synaptic plasticity and mimicked the characteristics of a nociceptor.
IEEE TRANSACTIONS ON ELECTRON DEVICES
(2023)
Article
Chemistry, Analytical
Yanfeng Fang, Zhixin Zhou, Yuhua Hou, Chenchen Wang, Xuwen Cao, Songqin Liu, Yanfei Shen, Yuanjian Zhang
Summary: We synthesized highly efficient polymeric carbon nitride (CN) films with fine-tuned electrochemiluminescence (ECL) emission from blue to green. The naked eye observable emission was significantly enhanced, and the cathodic ECL efficiency was greatly improved compared to the reference. The mechanism studies revealed crucial factors for the high ECL efficiency of CN. Based on the high ECL efficiency and different colors of ECL emission, a multiplexed ECL biosensor was constructed to detect miRNA-21 and miRNA-141 simultaneously with low detection limits.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Xuwen Cao, Caixia Zhu, Qing Hong, Xinghua Chen, Kaiyuan Wang, Yanfei Shen, Songqin Liu, Yuanjian Zhang
Summary: This study reports the influence of Fe leaching on the selectivity of the oxygen reduction reaction (ORR) during catalytic H(2)A oxidation using an Fe-N-C nanozyme. The leakage of Fe ions activated a new ORR pathway and Fenton-type H(2)A oxidation, explaining the contradictory mechanisms underlying H(2)A oxidation. The activation of certain N-C sites led to H2O2 production, which was the origin of the pro-oxidant effect of H(2)A.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Physical
Qichao Chen, Chengjie Lu, Boyu Ping, Guiyang Li, Jiayi Chen, ZhengMing Sun, Yuanjian Zhang, Qiushi Ruan, Li Tao
Summary: This study presents a novel approach for scalable solar-driven H2O2 evolution from water, which achieves a 2.1% apparent quantum yield and a 4.5-fold improvement in H2O2 production. The strategy involves optimizing spatial charge separation and suppressing surface reaction kinetics.
APPLIED CATALYSIS B-ENVIRONMENTAL
(2023)
Article
Biophysics
Kaiqing Wu, Yongjun Zheng, Ran Chen, Zhixin Zhou, Songqin Liu, Yanfei Shen, Yuanjian Zhang
Summary: Electrochemiluminescence (ECL) has various advantages and is widely used for sensing, imaging, and single cell analysis. Small organic ECL luminophores, with their biocompatibility and easy modification, play a key role in the performance of ECL applications. However, there is a lack of comprehensive reviews on small organic ECL luminophores. This review introduces the advantages, emission mechanisms, types, molecular characteristics, and applications of small organic ECL luminophores, and discusses the challenges and prospects in biosensing applications.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Multidisciplinary
Zhixin Zhou, Nina Lin, Yu Ouyang, Songqin Liu, Yuanjian Zhang, Itamar Willner
Summary: This article introduces the enzyme-free catalytic hairpin assembly (CHA) process for the emergence and evolution of nucleic acid networks known as constitutional dynamic networks (CDNs). The process is applied to assemble networks with varying complexities, functionalities, and spatial confinement, providing insights into the possible mechanistic pathways for the evolution of functional networks under prebiotic conditions.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Review
Chemistry, Multidisciplinary
Yuan Xu, Zhixin Zhou, Nankai Deng, Kangchun Fu, Caixia Zhu, Qing Hong, Yanfei Shen, Songqin Liu, Yuanjian Zhang
Summary: Nanozymes have emerged as cost-effective alternatives to natural enzymes and have gained increasing interest. To fully exploit their potential, rational structural engineering and explicit catalytic mechanisms at the molecular scale are required. Impressive progress has been made in mimicking the characteristics of natural enzymes, including the construction of metal active sites, binding pockets, scaffolds, and delicate allosteric regulation. This review introduces the state-of-the-art synthesis strategies and summarizes the general overview of the nanozyme mechanism, with a focus on adsorbed intermediates and descriptors that predict nanozyme activity. The emerging activity assessment methodology that illustrates the relationship between electrochemical oxygen reduction and enzymatic oxygen reduction is discussed, along with future opportunities and challenges to encourage further research in the field of nanozymes.
SCIENCE CHINA-CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Qing Hong, Hong Yang, Yanfeng Fang, Wang Li, Caixia Zhu, Zhuang Wang, Sicheng Liang, Xuwen Cao, Zhixin Zhou, Yanfei Shen, Songqin Liu, Yuanjian Zhang
Summary: This study reports an adaptable graphitic C6N6-based copper single-atom catalyst, capable of driving basic oxidation reactions of peroxidase substrates through a copper-oxo pathway and performing a second gain reaction triggered by light via a free hydroxyl radical pathway. The reactive oxygen-related intermediates of the same oxidation reaction enable consistent reaction conditions. The unique topological structure of CuSAC6N6 promotes intramolecular charge separation and migration, inhibiting negative interferences. The catalyst exhibits superior activity and gain compared to controls and is further applied to a glucose biosensor.
NATURE COMMUNICATIONS
(2023)
Article
Chemistry, Analytical
Caixia Zhu, Hong Yang, Xuwen Cao, Qing Hong, Yuan Xu, Kaiyuan Wang, Yanfei Shen, Songqin Liu, Yuanjian Zhang
Summary: This study demonstrates a direct two-electron oxidation pathway of TMB using SDS-based micelles, which overcomes the instability issue of TMB and enables reliable glucose measurement without the need for strong acid termination.
ANALYTICAL CHEMISTRY
(2023)
Review
Biotechnology & Applied Microbiology
Yongjun Cao, Qing Hong, Yuanjian Zhang
Summary: This review examines the principles, characteristics, advantages and disadvantages of recently developed advanced practical H-acid treatment technologies, and explores potential future development trends.
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Renjie Wu, Rongchuan Gu, Tamihiro Gotoh, Zihao Zhao, Yuting Sun, Shujing Jia, Xiangshui Miao, Stephen R. Elliott, Min Zhu, Ming Xu, Zhitang Song
Summary: In this study, we found that incorporating arsenic into GeS can significantly improve the performance of the ovonic threshold switching (OTS) selector. This is achieved by strengthening the arsenic-sulfur bonds and slowing down atomic migration after arsenic incorporation, which reduces leakage current and suppresses operational voltage drift.
NATURE COMMUNICATIONS
(2023)