Article
Spectroscopy
Mengwen Yi, Nan Zhang, Xiangjun Liu, Jing Liu, Xiangru Zhang, Yongbiao Wei, Dihua Shangguan
Summary: In this study, a hemicyanine-based off-on fluorescent probe, CO-H1, was developed for monitoring endogenous mitochondrial CO levels in living cells. CO-H1 exhibited significant fluorescence enhancement and red shift of absorption upon reaction with CO in the presence of PdCl2. The probe demonstrated low cytotoxicity, high sensitivity (detection limit of 0.048 μM), and good selectivity for CO. CO-H1 mainly targeted the mitochondria in living cells and successfully imaged exogenous/endogenous mitochondrial CO, indicating its potential for further studying the biological functions of mitochondrial CO in living cells.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Biochemistry & Molecular Biology
Junshuai Tang, Pingzhu Zhang, Zimeng Li, Yuehua Zhang, Hua Chen, Xiaoliu Li, Chao Wei
Summary: A simple ratiometric two-photon fluorescent probe RTFP was designed and synthesized for effective monitoring of carbon monoxide (CO) in living systems. The probe showed excellent selectivity and sensitivity towards CO, and successfully imaged endogenous CO in living cells and zebrafish.
BIOORGANIC CHEMISTRY
(2023)
Article
Chemistry, Analytical
Zhoupeng Zheng, Shumin Feng, Shengyi Gong, Guoqiang Feng
Summary: Gol-CO is an effective fluorescence probe for imaging CO in Golgi apparatus, with high selectivity and sensitivity. It can target Golgi apparatus in living cells and zebrafish, and visualize the level increase of CO under oxidative stress.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biochemical Research Methods
Lei Zhao, Rui Chen, Cheng Jia, Jiandong Liu, Guohua Liu, Tanyu Cheng
Summary: This study reports a probe PEC, based on BODIPY fluorescent dye, that selectively detects and tracks carbon monoxide in biological systems, with good stability and water solubility.
JOURNAL OF FLUORESCENCE
(2023)
Article
Chemistry, Analytical
Shiqing Zhang, Xinyue Mu, Jinbiao Zhu, Liqiang Yan
Summary: A metal-free coumarin-based fluorescent probe was developed for monitoring CO, showing good specificity and excellent sensitivity. The introduction of aryl amino group significantly increased the emission intensity at 450nm, leading to successful detection of CO in living cells.
Article
Spectroscopy
Yunyan Zhang, Yonghe Tang, Xiuqi Kong, Weiying Lin
Summary: Na-CM-ER, as a novel CO fluorescent probe with ER-targeting characteristics, exhibited satisfactory response speed, selectivity, photo-stability, and sensitivity to CO in vitro. It can monitor changes of exogenous/endogenous CO in living cells and possess an ER-targeting property, serving as a potentially useful molecular tool for imaging CO in cellular ER.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Chemistry, Analytical
Zimeng Li, Xu Jia, Pingzhu Zhang, Zhenbo Guo, Han Zhao, Xiaoliu Li, Chao Wei
Summary: A novel single-component fluorescent probe HCP was designed and synthesized to specifically detect hepatocellular carbon monoxide (CO) with high selectivity and significant fluorescent response. The probe could be used for imaging the endogenous CO release in HepG2 cells and zebrafish liver during acute liver injury.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Junli Peng, Qikai Ju, Baoshuai An, Zhengji Yin, Ningning Wei, Yanru Zhang
Summary: This study describes the design and biological applications of a novel fluorescence probe, SS-N-3, for the detection of hydrogen sulfide in vivo. The probe exhibits high selectivity and sensitivity to hydrogen sulfide, with strong anti-interference capacity. Additionally, the probe shows low cytotoxicity, improved photostability, and a large Stokes shift, making it an effective indicator of hydrogen sulfide levels in living cells.
Article
Chemistry, Analytical
Tienan Zang, Shudong Wang, Sa Su, Mengxu Gao, Qianqian Chen, Chenlu Liang, Jing Jing, Rubo Zhang, Xiaoling Zhang
Summary: A new fluorescent probe was designed to detect the key enzyme SQLE in living cells, showing specific response and ability to rapidly assess antifungal drug susceptibility. The probe provides a powerful tool for monitoring the content and distribution of SQLE.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Keqin Yang, Li Hou, Zhifang Li, Tianran Lin, Jianniao Tian, Shulin Zhao
Summary: A ratiometric fluorescent nanoprobe for detecting peroxynitrite was successfully constructed, showing selective accumulation in mitochondria and linear detection in the range of 0-6.0 µM. This nanoprobe enables ratiometric fluorescence imaging of endogenous peroxynitrite in cell mitochondria.
Article
Spectroscopy
Bingpeng Guo, Tianjiao Shen, Yifan Liu, Jing Jing, Changxiang Shao, Xiaoling Zhang
Summary: Esterase is primarily distributed in the endoplasmic reticulum (ER) and often overexpressed in cancer cells. To solve the limitations of existing probes, an ER-specific ratiometric fluorescent probe (ER-EST) is designed for detecting esterase in living cells. The ER-EST shows a ratiometric and red-shifted emission from 435 to 560 nm after hydrolysis by esterase, and it has a low detection limit and good biocompatibility.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Analytical
Jihua Zhang, Hao Zhang, Sujuan Ye, Xingxiang Wang, Lindong Ma
Summary: This study developed two smart binary star ratio probes for precise imaging and quantification of miRNAs. The probes showed good stability in living cells and were successfully used for accurate tracing and quantification of miR-203. The reported BSR probes have the potential to be a reliable tool for monitoring biomolecule dynamics in living cells.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Shengyi Gong, Enbo Zhou, Yijia Liu, Zhisheng Gui, Guoqiang Feng
Summary: A novel Pd2+-free near-infrared fluorescent probe CORM3-AE has been developed for detecting CORM-3 in live cells and in vivo. The allyl ether group in CORM3-AE can be cleaved by CORM-3 directly via an isomerization process, resulting in the release of a NIR fluorophore and distinct NIR fluorescence changes. CORM3-AE shows quick response, high sensitivity, and selectivity for CORM-3, and can track CORM-3 in live cells and animals with excellent imaging performance.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Shuwei Zhang, Xinyao Wang, Xuewen Wang, Ting Wang, Wenyi Liao, Yu Yuan, Gang Chen, Xiaodong Jia
Summary: An AIE fluorescent probe was synthesized for beta-gal detection, which exhibited selective and sensitive turn-on fluorescent responses and could be used for beta-gal imaging in cells.
ANALYTICA CHIMICA ACTA
(2022)
Article
Chemistry, Analytical
Hongkang Xu, Yingcui Bu, JunJun Wang, Min Qu, Jie Zhang, Xiaojiao Zhu, Gang Liu, Zhichao Wu, Guangmei Chen, Hongping Zhou
Summary: Lyso-pH, a coumarin-based fluorescent molecule, shows excellent pH sensitivity and targeting capability for monitoring intracellular pH changes, particularly providing important insights into the interaction between mitochondria and lysosome.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Qingfeng Xia, Shumin Feng, Jiaxin Hong, Guoqiang Feng
Summary: A novel probe ONOO-LysopH has been developed to detect peroxynitrite and track lysosomal pH changes in live cells. The probe is based on a new rhodamine derivative with near-infrared emission and large Stokes shift, showing quick, sensitive, and selective responses to peroxynitrite and acid, providing a potential multifunctional tool for live systems.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Haiyan Zhang, Xinyu Yin, Jiaxin Hong, Yingzhen Deng, Guoqiang Feng
Summary: CDCI-HClO is a novel reaction-based fluorescence probe that can rapidly, selectively, and sensitively detect hypochlorous acid, with a detection limit of about 4 nM. With a large Stokes shift of 190 nm, this probe was successfully applied to imaging hypochlorous acid in live cell, zebrafish, and living mice.
Article
Chemistry, Multidisciplinary
Enbo Zhou, Shengyi Gong, Qingfeng Xia, Guoqiang Feng
Summary: A near-infrared fluorescent probe CORM3-IR has been developed to effectively detect CORM-3 in living cells and in vivo, showing good water solubility and rapid, selective, and sensitive response to CORM-3. The probe can ratiometrically detect CORM-3 with fluorescence at 743 and 600 nm, and exhibits good NIR fluorescence bioimaging ability with low cytotoxicity, making it an effective tool for tracking and studying CORM-3 in the life system.
Article
Chemistry, Analytical
Shengyi Gong, Zhoupeng Zheng, Xiaogang Guan, Shumin Feng, Guoqiang Feng
Summary: A novel near-infrared mitochondria-targetable fluorescence probe MI-H2S was developed for the rapid and selective detection of H2S, with high contrast imaging ability in living systems and great potential in studying the role of H2S in inflammation. This probe can be applied effectively for visualization and study of H2S in mitochondria and in vivo.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Qingfeng Xia, Shumin Feng, Jiaxin Hong, Guoqiang Feng
Summary: The fluorescent probe NIR-Rh-LysopH has been designed for convenient tracking of lysosomal pH changes, showing rapid, sensitive, and reversible near-infrared fluorescence responses. This probe demonstrated low cytotoxicity and excellent lysosome-targeting ability in cell experiments, and successfully tracked lysosomal pH changes induced by drugs, heatstroke, and redox stress, offering promising applications in studying related life processes.
Article
Chemistry, Analytical
Shengyi Gong, Enbo Zhou, Yijia Liu, Zhisheng Gui, Guoqiang Feng
Summary: A novel Pd2+-free near-infrared fluorescent probe CORM3-AE has been developed for detecting CORM-3 in live cells and in vivo. The allyl ether group in CORM3-AE can be cleaved by CORM-3 directly via an isomerization process, resulting in the release of a NIR fluorophore and distinct NIR fluorescence changes. CORM3-AE shows quick response, high sensitivity, and selectivity for CORM-3, and can track CORM-3 in live cells and animals with excellent imaging performance.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Jiaxin Hong, Qianhua Li, Qingfeng Xia, Guoqiang Feng
Summary: DCIP, a dicyanoisophorone-based probe, shows great potential for high-fidelity imaging and tracking of lysosomes, with strong fluorescence, good cell permeability, and high photostability. It can achieve long-term and high spatiotemporal resolution imaging of lysosomes, as well as track lysosomal dynamics at extremely low concentrations.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Qianhua Li, Jiaxin Hong, Shumin Feng, Shengyi Gong, Guoqiang Feng
Summary: This study developed a polarity-sensitive cell membrane probe (COP) for sensitive and selective detection of cancer cells and tumors. The probe exhibits strong polarity-dependent fluorescence and excellent cell membrane targeting ability to selectively light up tumor cell membranes, indicating the polarity difference between cancer cell membranes and normal cell membranes.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Jiaxin Hong, Jinzheng Zhang, Qianhua Li, Guoqiang Feng
Summary: Complex intracellular processes require the cooperation of multiple organelles. Realtime tracking of their interplays is important for understanding biological processes. We report a single fluorescent probe (SFP) that can simultaneously differentiate and visualize three important organelles: mitochondria, lysosomes, and lipid droplets. This smart probe allows for the real-time and simultaneous tracking of the interplays of these organelles for the first time.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Yijia Liu, Shumin Feng, Shengyi Gong, Guoqiang Feng
Summary: Nonalcoholic fatty liver disease (NAFLD) is a global health issue. In this study, a dual-response fluorescent probe was developed to simultaneously detect peroxynitrite and viscosity, providing a potential new diagnostic method for NAFLD.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Qianhua Li, Wenlong Zhu, Shengyi Gong, Siyu Jiang, Guoqiang Feng
Summary: A viscosity-responsive plasma membrane probe (TPA-S) was developed and used to detect the viscosity difference between normal and tumor cell plasma membranes. The results showed that the plasma membrane of tumor cells is more viscous than that of normal cells, and TPA-S was successfully applied for tumor imaging.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Jiaxin Hong, Xiaogang Guan, Yao Chen, Xiaodong Tan, Shiya Zhang, Guoqiang Feng
Summary: Mitophagy is a crucial cellular process that plays vital roles in regulating cellular metabolism and mitochondrial quality control. Mitochondrial viscosity, closely associated with mitochondrial status, is a key microenvironmental index. To monitor mitophagy and mitochondrial viscosity changes, a probe called Mito-3 was developed. Mito-3, along with other probes, showed sensitivity to viscosity changes and fluorescence enhancement. These probes were successfully used to locate and visualize mitochondria, as well as monitor mitochondrial viscosity changes in cells. Additionally, Mito-3 was applied to visualize the mitophagy process induced by starvation and revealed an increase in mitochondrial viscosity during mitophagy.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Wenlong Zhu, Qianhua Li, Shengyi Gong, Guoqiang Feng
Summary: A highly sensitive cell membrane polarity probe (Cal-M) was developed for early cancer diagnosis. It has low cytotoxicity, good photostability, high sensitivity for polarity, and the ability to selectively light up cancer cells. Cal-M demonstrates excellent sensitivity and selectivity, and shows promise for early diagnosis of cancer.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Siyu Jiang, Jiaxin Hong, Shengyi Gong, Qianhua Li, Guoqiang Feng
Summary: With the widespread use of drugs, drug-induced acute kidney injury (AKI) has become a serious health concern worldwide. However, early diagnosis of drug-induced AKI is challenging due to the lack of effective biomarkers and noninvasive imaging tools. This study introduces a highly sensitive near-infrared fluorescence probe called DSMN, which reveals the relationship between SO2 and drug-induced AKI for the first time. The probe selectively and rapidly responds to SO3 (2-)/HSO3 (-) and successfully detects changes in the SO2 concentration in living cells and mice, making it a powerful tool for studying and diagnosing drug-induced AKI.
ANALYTICAL CHEMISTRY
(2023)
Article
Biophysics
Jiaxin Hong, Yijia Liu, Xiaodong Tan, Guoqiang Feng
Summary: High-fidelity imaging of LDs is crucial for studying their dynamic physiological processes and related diseases. In this study, we synthesized three fluorescent probes and found that DCIQ showed high polarity sensitivity and excellent imaging performance. DCIQ not only allowed real-time observation of the interplays between LDs and other organelles, but also enabled high-fidelity imaging of the dynamics and generation of LDs. Furthermore, DCIQ was successfully used to study the changes of LDs in ferroptosis and nonalcoholic fatty liver disease tissues.
BIOSENSORS & BIOELECTRONICS
(2023)
