Article
Food Science & Technology
Lifeng Wang, Hanwen Cao, Hui Jiang, Yan Fang, Donglei Jiang
Summary: In this paper, a novel liver lobule microtissue biosensor based on 3D bio-printing is developed for rapid determination of aflatoxin B1 (AFB1). A liver lobule model is constructed using methylacylated hyaluronic acid (HAMA) hydrogel, HepG2 cells, and carbon nanotubes, and 3D bio-printing is utilized for high-throughput and standardized preparation to mimic organ morphology and induce functional formation. The 3D bio-printed liver lobule microtissue is immobilized on a screen-printed electrode, and mycotoxin detection is performed using differential pulse voltammetry (DPV). This study presents a new mycotoxin detection method based on 3D printing technology, which exhibits high stability and reproducibility and holds promising application prospects in the field of food hazard detection and evaluation.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Chemistry, Analytical
Zicheng Fan, Xiaoyun Wei, Keke Chen, Ling Wang, Mingen Xu
Summary: Researchers have successfully created a 3D cell culture model that replicates liver cancer using dot extrusion printing technology. This model can be used for drug screening and studying disease mechanisms. By printing gelatin microbeads containing hepatocytes and endothelial cells, the researchers constructed liver lobule-like structures and successfully recreated the morphology of liver lobules. These models showed stronger drug resistance in anti-cancer drug screening.
Article
Engineering, Biomedical
Kun Du, Shibo Li, Chengpan Li, Ping Li, Chunguang Miao, Tianzhi Luo, Bensheng Qiu, Weiping Ding
Summary: This study develops a biomimetic liver lobule chip and establishes a more accurate on-chip NAFLD model, demonstrating for the first time the changes in lipid zonation in a liver lobule with the progression of NAFLD on a microfluidic chip. The application of the induced NAFLD model has shown promise in the prevention and reversibility of drug treatments, providing a platform for understanding NAFLD progression and identifying potential drug therapies.
ACTA BIOMATERIALIA
(2021)
Article
Medicine, General & Internal
Yan-zhong Han, Yu-ming Guo, Peng Xiong, Fei-lin Ge, Jing Jing, Ming Niu, Xu Zhao, Zhao-fang Bai, Hai-bo Song, Xiao-he Xiao, Jia-bo Wang
Summary: This study found that the aging population is more susceptible to liver-related ADRs, with the relative risk increasing exponentially with age. It is important to address risk management for older adults when using drugs with a high correlation to the risk of ADRs.
FRONTIERS IN MEDICINE
(2022)
Article
Cell Biology
Honglei Jian, Xin Li, Qianqian Dong, Shaonan Tian, Shuo Bai
Summary: Liver disease poses a serious threat to human life and health. Three-dimensional liver models, which mimic the structure and function of natural liver tissue, are now in high demand in the medical, scientific, and pharmaceutical fields. Constructing these models is extremely challenging due to the complex cellular composition and spatial arrangement of liver tissue. This study presents a droplet-based and layer-by-layer 3D bioprinting strategy to fabricate liver organoids with biomimetic lobule structure, demonstrating high cell viability and functionality. The findings have significant implications in the development of new drugs, disease modeling, and tissue regeneration.
CELL PROLIFERATION
(2023)
Article
Nanoscience & Nanotechnology
Shaofei Shen, Fangjuan Zhang, Yali Zhang, Yi Li, Yanbing Niu, Long Pang, Jinyi Wang
Summary: A multiconcentration gradient generator was constructed to investigate the effect of different drug concentration gradients on cells. The results showed that chemotherapy drugs inhibited the growth of cancer cells, and hepatoma cells exhibited higher drug resistance. This device provides a simple and reliable platform for studying optimal dosages at the single-cell level and screening chemotherapy regimens.
MICROSYSTEMS & NANOENGINEERING
(2023)
Article
Nanoscience & Nanotechnology
Shibo Li, Chengpan Li, Muhammad Imran Khan, Jing Liu, Zhengdi Shi, Dayong Gao, Bensheng Qiu, Weiping Ding
Summary: This study reports an approach for constructing hepatic sinusoids. Hepatic sinusoids are formed by demolding a self-developed microneedle array from a photocurable cell-loaded matrix in a large-scale liver-acinus-chip microsystem with a designed dual blood supply. The construction of hepatic sinusoids significantly enhances interstitial flows, leading to high cell viability, liver microstructure formation, and enhanced hepatocyte metabolism. Additionally, the study demonstrates the effects of oxygen and glucose gradients on hepatocyte functions and the application of the chip in drug testing. This work paves the way for the biofabrication of fully functionalized large-scale liver bioreactors.
MICROSYSTEMS & NANOENGINEERING
(2023)
Article
Gastroenterology & Hepatology
Yi-Shin Huang, Chen-Yi Wu, Ting-Tsung Chang, Cheng-Yuan Peng, Gin-Ho Lo, Chao-Wei Hsu, Chi-Tan Hu, Yi-Hsiang Huang
Summary: DILI patients combined with SCAR are common and have a high mortality rate in Taiwan. Allopurinol is the main culprit drug. Jaundice, acute kidney injury, and SJS/TEN are independent risk factors for mortality. HLA-B*5801, chronic kidney disease, and high drug dosage also contribute to the high mortality in cases of allopurinol-induced DILI with SCAR.
LIVER INTERNATIONAL
(2021)
Article
Nanoscience & Nanotechnology
G. Janani, Smriti Priya, Souradeep Dey, Biman B. Mandal
Summary: Bioengineering a liver model using bioprinting technology allows for accurate prediction of hepatotoxicity by achieving cellular crosstalk and hepatic metabolic functions. The model consists of tissue-specific liver extracellular matrix-based bioinks that support different liver cell types. The bioprinted liver model resembles native liver structure and shows enhanced metabolic functions, and can be used for drug toxicity assessment.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Biotechnology & Applied Microbiology
Lina Guo, Ziqing Zhu, Chuanzhou Gao, Kaiwen Chen, Shenzhou Lu, Hexin Yan, Wenming Liu, Mingqi Wang, Yanfang Ding, Lin Huang, Xiuli Wang
Summary: By developing SF-collagen I composite scaffolds with improved physical and chemical properties, a more biomimetic hepatic lobule-like model was successfully bioengineered, allowing iHepLPCs to differentiate into mature hepatocytes with enhanced liver functions and facilitating the arrangement of hepatocyte-endothelial cell interphase with improved polarity and bile capillary formation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Pharmacology & Pharmacy
Jingyu Fu, Hailong Qiu, Cherie S. Tan
Summary: Drug discovery is a costly and complex process, and efficient methods are needed to screen potential compounds and remove toxic substances. The liver plays a crucial role in determining drug efficacy and potential side effects. The liver-on-a-chip platform based on microfluidic technology has gained attention for its ability to predict drug metabolism and hepatotoxicity, as well as study pharmacokinetics and pharmacodynamics when combined with other organ-on-chip systems.
Review
Chemistry, Multidisciplinary
Xueping Lu, Liang Zhao, Xiayan Wang, Guangsheng Guo
Summary: As the largest internal organ in the human body, the liver plays a crucial role in metabolism. Understanding the physiological, biochemical, and pathological mechanisms of the liver is essential for the research and treatment of liver-related or metabolism-related diseases. The traditional two-dimensional in vitro cell culture model lacks the complexity and disease characteristics of the liver. Microfluidic technology, by designing appropriate microstructures and combining with three-dimensional liver tissue culture, can provide a more realistic microenvironment for studying liver-related biological mechanisms.
PROGRESS IN CHEMISTRY
(2023)
Article
Chemistry, Analytical
Liang Zhu, Xiaolong Zhang, Ruo Yuan, Yaqin Chai
Summary: A novel two-dimensional ladder-like DNA nanostructure (LDN) was constructed to enhance the catalytic efficiency of a cascade catalytic nanomachine (LDN-CCN) for ultrasensitive detection of target miRNA-21. The electrochemical biosensing platform realized sensitive detection with a linear range from 100 aM to 10 nM and a detection limit as low as 48.5 aM. This study provides insights into exploring novel functional DNA nanostructures and high-performing mimic enzyme cascade catalytic platforms for applications in biological fields and early disease diagnosis.
ANALYTICAL CHEMISTRY
(2022)
Review
Plant Sciences
Rui Zheng, Yang Sun, Xiaoyu Zhang, Chen Zhao, Pengqian Wang, Shiqi Chen, Zhao Chen, Ruijin Qiu, Aihua Liang, Hongcai Shang
Summary: This comprehensive review examines the clinical features of adverse events (AEs) associated with the combination of XYP and RB. The review analyzes data from randomized controlled trials, cohort studies, case-control studies, case reports, case series, and the National Adverse Drug Reaction Monitoring Information System. The most common AE reported is skin and appendage reactions, with a majority of cases being pseudo-allergic reactions. The study recommends increased awareness of the safety of the XYP-RB combination treatment, especially in children, and the standardization of medication protocols.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Shengnan Ya, Weiping Ding, Shibo Li, Kun Du, Yuanyuan Zhang, Chengpan Li, Jing Liu, Fenfen Li, Ping Li, Tianzhi Luo, Liqun He, Ao Xu, Dayong Gao, Bensheng Qiu
Summary: This study develops a lifelike bionic liver lobule chip with perfusable hepatic sinusoid networks achieved using microflow-guided angiogenesis methodology and regulated oxygen concentration. The design produces more bionic liver microstructures, higher metabolic abilities, and longer lasting hepatocyte function than other liver-on-a-chip techniques. The unique micropillar design and oxygen concentration play key roles in guiding the assembly of hepatic sinusoid and prolonging hepatocyte function, demonstrating a broad range of applications for chronic and acute hepatotoxicity testing and tumor growth replication.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Physical
Yajuan Deng, Tianbao Li, Qin Tu, Jinyi Wang
Summary: Flexible and wearable hydrogel sensors have attracted significant interest due to their potential applications in human-machine interfaces, human-activity monitoring, and personal healthcare diagnosis. This study presents a healable, adhesive, and conductive hydrogel sensor that incorporates polydopamine decorated carbon nanotubes (PDA-CNTs) and FeCl3 into an acrylamide-co-acrylic acid polymer matrix. The hydrogel sensor exhibited good stretchability and high sensitivity, as well as fast and repeatable self-healing ability. It also demonstrated stable and accurate monitoring of human motions.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2023)
Article
Chemistry, Physical
Tao Wang, Jinyi Wang, Pengjie Wang, Fan Wang, Liping Liu, Haijiao Guo
Summary: In this study, a 3D numerical model and quantitative parameters were established to investigate the liquid flow uniformity in a concave-convex bi-polar plate (CCBP) electrolyzer. The simulated velocity field revealed significant non-uniform flow in the CCBP electrolyzer, which was validated by a flow visualization experiment. The residence time distribution (RTD) analysis using the CFD coupled particle tracing method can be applied as a criterion for electrolyzer structure design, operation optimization, and scale-up.
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
(2023)
Article
Agriculture, Dairy & Animal Science
Tianxiang Chen, Baoyuan Liu, Yiyang Chen, Xueting Wang, Meimei Zhang, Xukun Dang, Qin Zhao, En-Min Zhou
Summary: A rapid and reliable competitive ELISA (cELISA) was developed to detect avian HEV antibodies, which showed good specificity. The epitope recognized by Nb49 was located in aa 593-604 of the avian HEV capsid protein, and the peptide TFPS in aa 601-604 was essential for binding.
Article
Chemistry, Multidisciplinary
Jinyi Wang, Ling Zhou, Daying Guo, Xueyu Wang, Guoyong Fang, Xi'an Chen, Shun Wang
Summary: A flower-shaped NiS2-WS2 heterojunction was prepared to improve the reaction kinetics of sulfur in lithium-sulfur batteries. The heterojunction enhanced mass and charge transfer, and acted as an electron receptor and a donor for bidirectional catalytic activity. The results showed excellent discharge performance and cycle stability.
Article
Chemistry, Applied
Bin Yang, Jinyi Wang, Yuheng Qi, Daying Guo, Xueyu Wang, Guoyong Fang, Xi'an Chen, Shun Wang
Summary: A systematic modification of separators using bismuth oxycarbonate nanoflowers decorated conductive carbon is proposed, which effectively addresses the shuttle effect of polysulfides in lithium-sulfur batteries and improves battery performance and cycling stability.
JOURNAL OF ENERGY CHEMISTRY
(2023)
Article
Chemistry, Analytical
Lingtong Zhou, Yuanchang Li, Jingcheng Xiao, Shu-Wei Chen, Qin Tu, Mao-Sen Yuan, Jinyi Wang
Summary: In recent years, there has been a rapid increase in interest for wearable and stretchable multifunctional sensors. The development of practical and versatile devices has faced challenges in achieving self-healing and adhesion to target objects. This study prepared a hydrogel (LM-SA-PAA) with ultrastretchable, self-healing, self-adhesive, and high-sensitivity sensing capabilities, allowing for conformal contact with the skin during dynamic movements. The hydrogel's excellent self-healing properties arise from its double cross-linked networks, and its adhesion is ensured by the microgel shell formed by alginate. The hydrogel showed strain sensitivity and the ability to detect various human motions and electrocardiographic monitoring, making it a promising option for human-machine interface compatibility and medical monitoring.
ANALYTICAL CHEMISTRY
(2023)
Article
Nanoscience & Nanotechnology
Shaofei Shen, Fangjuan Zhang, Yali Zhang, Yi Li, Yanbing Niu, Long Pang, Jinyi Wang
Summary: A multiconcentration gradient generator was constructed to investigate the effect of different drug concentration gradients on cells. The results showed that chemotherapy drugs inhibited the growth of cancer cells, and hepatoma cells exhibited higher drug resistance. This device provides a simple and reliable platform for studying optimal dosages at the single-cell level and screening chemotherapy regimens.
MICROSYSTEMS & NANOENGINEERING
(2023)
Article
Engineering, Manufacturing
Yong-Hua Shi, Zi-Shun Wang, Xi-Yin Chen, Yan-Xin Cui, Tao Xu, Jin-Yi Wang
Summary: This paper proposes a two-stage model, called the segmentation-LSTM model, to monitor the penetration state of K-TIG welding in real time. The system extracts 9 weld pool geometric features with a segmentation network and extracts the weld gap using a traditional algorithm. These 10-dimensional features are then input into the LSTM model for penetration state prediction, with a recognition accuracy of 95.2%.
ADVANCES IN MANUFACTURING
(2023)
Article
Microbiology
Yiyang Chen, Meimei Zhang, Tianxiang Chen, Jiaxi Wang, Qin Zhao, En-Min Zhou, Baoyuan Liu
Summary: A novel nanobody-based competitive enzyme-linked immunosorbent assay (cELISA) was developed to detect Hepatitis E virus (HEV) antibodies in different species. The cELISA showed high specificity, sensitivity, and convenience, making it a promising technique for serological evaluation and diagnosis of HEV infection.
MICROBIOLOGY SPECTRUM
(2023)
Article
Engineering, Environmental
Qiuyi Lu, Zhoujie Wang, Jingyi Wang, Lei Xie, Qi Liu, Hongbo Zeng
Summary: Humic acid can form metal-HA complexes with a variety of divalent cations, and the interaction mechanism between them was studied at the nanoscale. The presence of Ca2+ enhances the aggregation and adhesion of HA, while excessive Ca2+ suppresses these behaviors. Cu2+ shows the highest adhesion energy due to its synergistic bonding with both oxygen and amine sites of HA.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Environmental Sciences
Zhoujie Wang, Huadong Tian, Jing Liu, Jingyi Wang, Qingye Lu, Lei Xie
Summary: Adsorption of Cd(II) on serpentine was investigated under typical environment conditions, involving common environmental anions and cations. The adsorption was negligibly affected by anion type, but was specifically modulated by cations. Mono- and divalent cations enhanced the adsorption by weakening electrostatic double layer repulsion, while trivalent cations suppressed the adsorption due to competitive adsorption. Fe3+ and Al3+ prevented Cd(II) adsorption by binding surface active sites, forming more stable inner-sphere complexes. This study provides insights into the influence of interfacial ion specificity on Cd(II) adsorption in terrestrial and aquatic environments.
ENVIRONMENTAL POLLUTION
(2023)
Article
Chemistry, Analytical
Jingcheng Xiao, Jingjing Jiang, Zexu Zhao, Jiahao Guo, Jinyi Wang
Summary: In this study, a distance-based microfluidic paper-based analytical device (muPAD) modified with carboxyl-containing polystyrene nanoparticles (PS-muPAD) was developed to improve the clarity of the discoloration boundary. The modified device showed improved accuracy in calculating analyte content.
ANALYTICAL METHODS
(2023)
Meeting Abstract
Urology & Nephrology
Eric Davenport, Xiaolei Zhou, Jinyi Wang, Sasikiran Nunna, Dorothee Oberdhan, Ancilla Fernandes, Djalila Mekahli
AMERICAN JOURNAL OF KIDNEY DISEASES
(2023)
Article
Engineering, Chemical
Anrong Yao, Jinghong Qiu, Yafang Wang, Haoyi Hu, Cuimin Huang, Jingyi Wang, Siyao Tian, Lin Yang, Shaojian Lin, Jianwu Lan
Summary: In this study, MIL-88B loaded montmorillonite (MMT) and cellulose nanocrystals (CNC) were incorporated into gelatin aerogel to create a multifunctional and reinforced bio-aerogel, MMCG aerogel, for the removal of organic pollutants. The MMCG aerogel exhibited highly efficient adsorption capacity and showed improved removal performance for all organic contaminants by triggering the Fenton reaction with the addition of H2O2.
SEPARATION AND PURIFICATION TECHNOLOGY
(2024)
Article
Chemistry, Physical
Dianlin Wang, Jiang He, Zili Xiao, Jingyi Wang
Summary: This article discusses the limitations on molar ratios and concentrations in Isothermal titration calorimetry (ITC) caused by the volumes and concentrations of syringe and sample cell. The study explores the neglected aspect of the molar ratios and concentrations of sodium dodecylsulfate (SDS) and imidazolium-based ILs in self-assembly structure transitions. The variations in macroscopic properties and molecular ratios of surfactants responsible for constructing vesicle structures in different alkyl chain lengths systems are investigated using enthalpy changes and cryogenic Transmission Electron Microscopy (cryo-TEM).
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2024)