期刊
ANALYTICAL CHEMISTRY
卷 88, 期 9, 页码 4600-4604出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.6b00772
关键词
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资金
- National Natural Science Foundation of China [21475066, 81401463, 81402896]
- Fundamental Research Funds for Central Universities (China)
- Thousand Youth Talents Plan of China
Quantifying trace microRNAs (miRNAs) is extremely important in a number of biomedical applications but remains a great challenge. Here we present an enzyme-free amplification strategy called plasmon-enhanced hybridization chain reaction (PE-HCR) for quantifying trace miRNAs with an outstanding linear range from 1 fM to 1 pM (r(2) = 0.991), along with a detection limit of 0.043 fM (1300 molecules in 50 mu L of sample). The merits of the PE-HCR assay, including high sensitivity and specificity, quantitative detection, no enzyme involvement, low false positives, and easy-to-operate procedures, have been demonstrated for high-confidence quantification of the contents of miRNAs in even single cancer cells. The PE-HCR assay may open up new avenues for highly sensitive quantification of biomarkers and thus should hold great potentials in clinical diagnosis and prognosis.
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