Multidimensional Analysis of 16 Glucose Isomers by Ion Mobility Spectrometry

Diastereomeric adducts comprising an enantiomerically pure monosaccharide analyte, a peptide, and/or an amino acid and a divalent metal ion (for 16 different monosaccharide isomers) are generated by electrospray ionization and analyzed by combined ion mobility spectrometry-mass spectrometry (IMS-MS) techniques. Mobility distributions of [L-Ser + M + H]}(+) (where L-Ser is L-serine and M is a given monosaccharide), [L-Phe-Gly + M + H](+) (where L-Phe-Gly is L-phenylalanine glycine), and [Mn-II + (L-Phe-Gly - H) + M](+) complex ions are used to determine collision cross sections (ccs in angstrom(2)), and groups of cross sections for different clusters are proposed as means of identifying the sugar isomers. Within one type of complex, variations in ccs do not always allow delineation between the 16 glucose isomers, but interestingly, when ccs of three different ions are combined as a spatial vector, enantiomers are partially resolved. As a result of this analysis, L-glucose, n-glucose, L-allose, n-allose, n-gulose, n-galactose, and L-mannose are delineated, and for all eight enantiomeric pairs, D and L entities display different coordinates. In addition, different combinations of amino acids, peptide, and metal ions are surveyed, and the potential for yielding unique coordinates for the generated diastereomeric complexes is assessed.