Article
Chemistry, Analytical
Yuliang Cheng, Guowen Li, Xiufang Huang, Zhijuan Qian, Chifang Peng
Summary: A label-free fluorescent detection method for glyphosate based on DNA-Ag NCs and a Cu2+-ion-modulated strategy was developed, achieving ultrasensitive detection of glyphosate. The method showed a linear response in the range of 1.0-50 ng/mL with a LOD of 0.2 ng/mL, demonstrating high application potential for glyphosate detection.
Article
Biotechnology & Applied Microbiology
Si Yang, Chenxi Li, Hongyan Zhan, Rong Liu, Wenliang Chen, Xiaoli Wang, Kexin Xu
Summary: In this study, a label-free fluorescent biosensor for the detection of tetracycline (TET) was developed using specific aptamer-templated silver nanoclusters (AgNCs). The binding of TET to the aptamer led to a conformational change, resulting in the transformation of AgNCs into larger silver nanoparticles (AgNPs) and a decrease in fluorescence. The proposed biosensor exhibited high sensitivity and selectivity for TET detection in milk, with a limit of detection of 11.46 ng/mL and a linear range of 20 ng/mL-10 g/mL. These findings demonstrate the potential of the biosensor for quantitative determination of TET in food samples and expand the application of DNA-AgNCs in related fields.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Biochemical Research Methods
Hong Zhang, Pengfei Liu, Huifang Wang, Xiaoming Ji, Mingqin Zhao, Zhaopeng Song
Summary: The article proposes a simple and label-free strategy for the sensitive detection of lysozyme based on fluorescence quenching of DNA/AgNCs by (+)AuNRs. By utilizing a DNA template with specific sequences for AgNCs synthesis and lysozyme recognition, the method achieves accurate lysozyme detection and shows a linear relationship between fluorescence signal recovery and lysozyme concentration in the range of 10 pM-2.0 nM, with satisfactory results in human serum samples.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Xiao Yang, Yang Huang, Siyi Yang, Miao Tang, Juan Liu, Jinhui Shen, Huanbao Fa, Danqun Huo, Changjun Hou, Mei Yang
Summary: A fluorescent biosensor was designed for the label-free detection of ctDNA based on the stronger affinity of MnO2 nanosheets towards ssDNA. The biosensor utilized MnO2 NSs as a nanoenzyme to oxidize dopamine into fluorescent polydopamine nanoparticles as a signal. The sensor demonstrated high sensitivity, stability, and reproducibility, and was successfully applied for the detection of ctDNA in serum samples and cell lysates.
Article
Chemistry, Analytical
Xiao Yang, Yang Huang, Siyi Yang, Miao Tang, Juan Liu, Jinhui Shen, Huanbao Fa, Danqun Huo, Changjun Hou, Mei Yang
Summary: In this study, a fluorescent biosensor based on MnO2 nanosheets was designed for label-free detection of ctDNA. The biosensor exhibited reliable readout in a short time and showed excellent specificity, stability, and reproducibility. The application of this biosensor in liquid biopsy has great potential for biomarker detection.
Article
Engineering, Environmental
Hao Wu, Hongyong Wang, Jun Wu, Guoqing Han, Yaling Liu, Pei Zou
Summary: A novel label-free fluorescent aptasensor based on exonuclease-assisted triple recycling amplification was demonstrated for sensitive detection of AFB1, achieving high sensitivity and performance in real samples.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Mark A. A. Reed, Yulia V. V. Gerasimova
Summary: The study introduces a single-tube biosensor for real-time detection of bacterial pathogens with multiplex capabilities. The biosensor utilizes a 3WJ nucleic acid structure to enable fluorescent detection of RNA targets, showing excellent selectivity within 30 minutes. The sensor is suitable for point-of-care scenarios and allows for multiplex detection of multiple bacterial species by utilizing different light-up aptamers.
FRONTIERS IN CHEMISTRY
(2022)
Article
Biochemical Research Methods
Xi Yang, Chaoyang Gong, Yanqiong Wang, Yanhua Luo, Yun-Jiang Rao, Gang-Ding Peng, Yuan Gong
Summary: By utilizing a sequential bioconjugation protocol and miniaturized hollow optical fiber, reagents can be stored and transferred in biosensing, improving sensitivity, and reducing sample consumption and cost.
Review
Chemistry, Inorganic & Nuclear
Lei Feng, Jing Ning, Xiangge Tian, Chao Wang, Zhenlong Yu, Xiaokui Huo, Tian Xie, Baojing Zhang, Tony D. James, Xiaochi Ma
Summary: Cytochrome P450 (CYP) enzymes are haem-containing proteins mainly involved in the oxidative metabolism of various chemicals. Fluorescent probes are useful molecular tools for selectively detecting CYP activity, facilitating important insights into the activity expression of CYP enzymes.
COORDINATION CHEMISTRY REVIEWS
(2021)
Article
Chemistry, Analytical
Jing-Xuan Tian, Yan-Zhao Fang, Rui Yu, Zi-Yan Zhang, Yan-Tong Zhuo, Jia-Yu He, Shuang Wu, Qiang Xiao, Xiang-Juan Kong
Summary: By constructing a novel nanoplatform using PDA NPs as an effective signal reporter, the method demonstrated high sensitivity and desirable selectivity for the detection of ALP. This approach not only could be used to screen inhibitors of ALP, but also successfully detect ALP activity in human serum samples.
ANALYTICAL METHODS
(2021)
Article
Chemistry, Multidisciplinary
Wen Wen, Zhongping Li, Xu Wang, Xiaoyan Du, Guangming Wen, Li Li
Summary: Polyethyleneimine stabilized palladium nanoclusters were synthesized as fluorescent nanosensor for oxytetracycline detection, showing good stability and photobleaching resistance. The detection limit for OTC was calculated to be 22 nM, with a linear relationship between Pd NCs fluorescence quenching and OTC concentration. This work establishes a new nanosensing analysis method for OTC detection.
Article
Chemistry, Multidisciplinary
Parimah Aminfar, Goonay Yousefalizadeh, Emily Steele, Juan Chen, Gang Zheng, Kevin G. Stamplecoskie
Summary: Aqueous gold nanoclusters (AuNCs) have potential applications in biomedical imaging and therapy due to their unique electronic structures. However, their optical impurity and polydispersity pose challenges for isolation of the most stable structure and potential clinical applications. This study reports optically pure Au16L14 clusters, assessed by fluorescence excitation-emission matrix (EEM) spectroscopy and parallel factor (PARAFAC) analysis. The reactivity of the excited state with molecular oxygen was also investigated, showing that Au16L14 clusters generate type I reactive oxygen species (ROS), making them effective sensitizers for photodynamic therapy.
Article
Biochemical Research Methods
Kwan-Ling Wu, Melisa Martinez-Paniagua, Kate Reichel, Prashant S. Menon, Shravani Deo, Badrinath Roysam, Navin Varadarajan
Summary: This study aims to improve the detection of apoptosis using label-free methods. By training a ResNet50 network, nanowells containing apoptotic bodies were successfully identified and the onset of apoptosis was accurately predicted. The method achieved good results in apoptotic body segmentation, enabling the identification of apoptotic cells. Compared to traditional Annexin-V staining, this method detected a higher number of apoptosis events.
Article
Engineering, Electrical & Electronic
Hira Khalid, Muhammad Farooq Khan, Bilal Ahmad, Muhammad Ismail, Muhammad Zahid, Ahmed Ismail
Summary: In this study, carbon nanodots (CNDs) were synthesized using grass as a carbon source and characterized by various techniques. The grass-derived CNDs exhibited strong absorption and fluorescence emission, and showed excellent sensitivity and selectivity for the detection of Cu2+ ions.
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN ELECTRONICS
(2022)
Article
Chemistry, Analytical
Yucui Hou, Xiaoling Lu, Jie Yang, Chunhua Tang, Hanbing Jiang, Tongji Cai, Meilun Chen, Zheng Wei, Peng Yu
Summary: This study developed an unlabeled fluorescent sensor based on SYBR Green I for the detection of testosterone. The sensor showed high specificity and good performance, allowing for quantitative detection in real water samples, such as tap water and river water. It provides a convenient and efficient alternative method for the quantitative detection of testosterone in the environment.
ANALYTICAL METHODS
(2023)
Article
Biochemistry & Molecular Biology
Maryam Besharati, Mahmoud Amouzadeh Tabrizi, Fatemeh Molaabasi, Reza Saber, Mojtaba Shamsipur, Javad Hamedi, Saman Hosseinkhani
Summary: Two biosensing methods utilizing NAD(P)H-dependent TetX2 enzyme activity and thionine were developed to detect tetracycline in milk. The methods showed great potential for being portable instruments for on-site TC measurements.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Zahra Gharehdaghi, Rahmatollah Rahimi, Seyed Morteza Naghib, Fatemeh Molaabasi
Summary: In this study, two types of Cu-MOF-based nanocomposites, GO/Cu-TCPP and Fe3O4@Cu-3(BTC)(2), were investigated for loading and pH-sensitive release of doxorubicin (DOX). It was found that GO/Cu-TCPP had a higher adsorption capacity for DOX compared to Fe3O4@Cu-3(BTC)(2). The release of DOX from both nanocomposites was pH-sensitive, with GO/Cu-TCPP releasing a higher percentage of DOX at pH 5 than at pH 7.4. The difference in drug release between the two nanocomposites was attributed to their differences in drug loading capacity. The results indicate that both GO/Cu-TCPP and Fe3O4@Cu-3(BTC)(2) nanocomposites have low toxicity and good biocompatibility.
JOURNAL OF THE IRANIAN CHEMICAL SOCIETY
(2022)
Article
Nanoscience & Nanotechnology
Soroush Moasses Ghafary, Elnaz Rahimjazi, Hadiseh Hamzehil, Sayed Mostafa Modarres Mousavi, Maryam Nikkhah, Saman Hosseinkhani
Summary: There is a need for a novel targeted drug delivery system to overcome the drawbacks of conventional chemotherapy. In this study, nano-carriers based on MiRGD peptides and graphene quantum dots were used for targeted delivery of two different drug models. The results indicate that the prepared nano-composites are suitable for targeted drug delivery and tracking in cancer cells.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2022)
Review
Chemistry, Physical
Yasaman-Sadat Borghei, Saman Hosseinkhani, Mohammad Reza Ganjali
Summary: This review provides a guideline for physicist/chemist/biologist/physician in the introduction of metallic nanocluster applications in biomedicine. It introduces the physicochemical properties of metallic nanoclusters and the potential biomedical opportunities, guiding researchers in developing new scaffolds for biomedical engineering.
PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION
(2022)
Article
Biochemistry & Molecular Biology
Yasaman-Sadat Borghei, Saman Hosseinkhani
Summary: DNA-functionalized gold nanoparticles hold great promise in biomedical applications. A novel approach of in-situ synthesis of gold nanoparticles on polyguanine strands is reported, leading to the formation of polyvalent DNA-functionalized gold nanoparticles. This method demonstrates general applicability and shows higher attractiveness in biomedical diagnosis and specific sequence detection.
PHOTOCHEMISTRY AND PHOTOBIOLOGY
(2022)
Article
Toxicology
Jalil Mehrzad, Taghi Zahraei Salehi, Alireza Khosravi, Saman Hosseinkhani, Yaser Tahamtani, Ensiyeh Hajizadeh-Saffar, Maedeh Moazenchi, Amir Mohammad Malvandi
Summary: This study analyzed the effects of aflatoxins on rat pancreatic islet cells, finding that these environmental toxins can impair the quality and function of the cells, potentially leading to endocrine disorders such as type-2 diabetes.
Article
Multidisciplinary Sciences
Amir Amiri-Sadeghan, Ali Dinari, Soheila Mohammadi, Tayebeh Zohrabi, Reza Khodarahmi, Saman Hosseinkhani, Jungwon Yoon
Summary: The photo-physical properties of metal nano clusters are sensitive to their surrounding medium, and this sensitivity, combined with the unique properties of self-assembled structures, opens up new opportunities for sensing applications.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Yasaman-Sadat Borghei, Saman Hosseinkhani, Mohammad Reza Ganjali
Summary: This review focuses on the basic physicochemical properties of plasmonic nanoparticles and their applications in biomedical and tissue engineering. It aims to provide a comprehensive and authoritative overview to the scientific community. The review introduces the fundamental characteristics of nanomaterials and illustrates their applications in the field through selected case studies.
JOURNAL OF ADVANCED RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Soroush Moasses Ghafary, Paula M. Soriano-Teruel, Shima Lotfollahzadeh, Monica Sancho, Eva Serrano-Candelas, Fatemeh Karami, Stephen J. Barigye, Ivan Fernandez-Perez, Rafael Gozalbes, Maryam Nikkhah, Mar Orzaez, Saman Hosseinkhani
Summary: This study identified QM380 and QM381 as inhibitors of NLRP3(PYD) homo-oligomerization, which interfere with the formation of inflammasomes and reduce inflammatory responses. Spectroscopic techniques and computational docking analyses confirmed the experimental results and predicted possible mechanisms underlying the inhibition of NLRP3(PYD) homo-interactions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Optics
Yasaman-Sadat Borghei, Saman Hosseinkhani
Summary: This review focuses on the physico-chemical basis of semiconductor quantum dots in various medical applications, emphasizing more on the strategy and method rather than the target molecules.
JOURNAL OF LUMINESCENCE
(2022)
Article
Multidisciplinary Sciences
Shima Lotfollahzadeh, Elaheh Sadat Hosseini, Hooman Mahmoudi Aznaveh, Maryam Nikkhah, Saman Hosseinkhani
Summary: This study successfully fabricated a promising nanohybrid system to improve the therapeutic efficacy of TRAIL. By conjugating TRAIL with graphene quantum dots, the study demonstrated enhanced functional stability and effective anticancer activity.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Multidisciplinary
Y-S Borghei, S. Hosseinkhani, M. R. Ganjali
Summary: Magnetic nanoparticles (MNPs) have biomedical applications that can be classified based on their unique properties in the presence or removal of an external magnetic field. Magnetite nanoparticles, with their unique physicochemical and catalytic properties, have gained increasing attention in medical fields for diagnosis, imaging, therapy, and cellular/tissue engineering.
MATERIALS TODAY CHEMISTRY
(2022)
Article
Nanoscience & Nanotechnology
Maryam Hosseini, Zahed Ahmadi, Amirhosein Kefayat, Fatemeh Molaabasi, Anita Ebrahimpour, Yousef Naderi Khojasteh Far, Mehdi Khoobi
Summary: In this study, a helical structure of gold nanoparticles was engineered using Spirulina platensis as a biotemplate for the treatment and diagnosis of cancer. The biohybrid showed excellent imaging performance and tumor suppression effects, as well as biodegradability and good biocompatibility.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Analytical
Fatemeh Molaabasi, Amirhosein Kefayat, Abbas Ghasemzadeh, Mojdeh Amandadi, Mojtaba Shamsipur, Mozhgan Alipour, Seyyed Ebrahim Moosavifard, Maryam Besharati, Saman Hosseinkhani, Ramin Sarrami-Forooshani
Summary: A label-, antibody-, enzyme-, and amplification-free ratiometric fluorescent biosensor has been developed for the rapid and low-cost diagnosis of COVID-19. The biosensor exhibits high sensitivity and specificity, and can detect the virus using isolated RNA samples.
ANALYTICAL CHEMISTRY
(2022)
Article
Nanoscience & Nanotechnology
Mojtaba Shamsipur, Elaheh Babaee, Mohammad-Bagher Gholivand, Fatemeh Molaabasi, Farimah Mousavi, Ali Barati, Behnam Hajipour Verdom, Behnaz Shojaedin-Givi, Hossein Naderi-Manesh
Summary: In this study, a new fluorescence probe based on insulin-capped gold nanoclusters (Ins/Au-NCs) was developed for selective detection of Cu2+ ions and glutathione (GSH). The probe showed high sensitivity and stability. Cu2+ ions caused fluorescence quenching of Ins/Au-NCs, which could be recovered in the presence of GSH. The probe had low detection limits and broad linear ranges for Cu2+ ions and GSH. It also showed promising application in detecting low levels of Cu2+ ions and GSH in water and human serum samples.
ACS APPLIED NANO MATERIALS
(2023)
