Article
Engineering, Chemical
You Wu, Wenjing Yang, Fanhui Zhu, Peijin Liu, Yan Ba
Summary: This paper explores the particle manipulation technology based on SSAW using CFD and DEM methods, validating the accuracy and advantages of the model while revealing the mechanism of particle separation.
Article
Biochemical Research Methods
Hyeono Nam, Hyung Jin Sung, Jinsoo Park, Jessie S. Jeon
Summary: This study investigated the behaviors of microparticles and cancer cells inside a sessile droplet using surface acoustic waves. It explored the impact of droplet volume and contact angle on the behaviors of the particles, and demonstrated the non-contact manipulation property of acoustic waves, showing a biocompatible and structure-free platform configuration. The research also showed enhanced expression of E-cadherin in three-dimensional aggregated culture models of cancer cells and increased efficacy of the anticancer drug tirapazamine in aggregated cancer cells due to low oxygen levels.
Article
Mathematics, Interdisciplinary Applications
Xueye Chen, Honglin Lv, Yaolong Zhang
Summary: This paper investigates the separation of micron-sized particles using stand-ing surface acoustic waves (SSAW) and proposes a two-step sorting model. The study focuses on the analysis of the SSAW device, the comparative analysis of different piezoelectric substrates, and the effects of fork-finger pairs and input voltage of the interdigital transducer on the stability of the sorting process. The research result provides a theoretical basis for practical applications such as cell sorting and drug detection.
CHAOS SOLITONS & FRACTALS
(2022)
Article
Acoustics
Erfan Taatizadeh, Arash Dalili, Pamela Ines Rellstab-Sanchez, Hamed Tahmooressi, Adithya Ravishankara, Nishat Tasnim, Homayoun Najjaran, Isaac T. S. Li, Mina Hoorfar
Summary: Acoustofluidics is an effective and novel method for cellular and particle separation and purification, offering advantages over traditional approaches. This study utilized two-dimensional numerical simulations to optimize platform design for improved separation efficiency and reproducible experimental outcomes.
ULTRASONICS SONOCHEMISTRY
(2021)
Article
Engineering, Electrical & Electronic
Guojun Liu, Wanghao Shen, Yan Li, Hong Zhao, Xinbo Li, Conghui Wang, Fang He
Summary: This paper proposes a continuous separation method for multiple particles with the same volume but different densities based on a microfluidic chip. By using standing surface acoustic waves and hydrodynamic separation technology, continuous and effective separation of particles can be achieved. Experimental results show that this method can efficiently separate particles with different densities, with potential applications in biological research, disease diagnosis, and clinical practice.
SENSORS AND ACTUATORS A-PHYSICAL
(2022)
Article
Nanoscience & Nanotechnology
Sebastian Sachs, Hagen Schmidt, Christian Cierpka, Jorg Konig
Summary: This study investigates the dynamical behavior of non-spherical particles manipulated and separated by high-frequency standing surface acoustic waves (sSAW) in fluids. The study reveals an alignment phenomenon of particles orthogonal to the pressure nodes of sSAW and proposes a three-dimensional numerical model to predict the acoustic forces and torques acting on the particles. The numerical model, considering the acoustically induced streaming around the particle, shows excellent agreement with experimental findings and suggests a correlation between particle shape and acoustic force.
MICROFLUIDICS AND NANOFLUIDICS
(2023)
Review
Chemistry, Analytical
Wei Wei, Yaping Wang, Zhaoxun Wang, Xuexin Duan
Summary: Acoustofluidics, a branch of microfluidics, has developed rapidly in recent decades, with applications in various biomedical and bioanalytical fields. The manipulation of liquids and bioparticles using acoustic pressure and acoustic streaming effects is the main focus of acoustofluidics. While the use of acoustic radiation force has been extensively studied, the recent development of new piezoelectric devices has sparked interest in particle manipulations using drag force induced by acoustic streaming. This review article summarizes the recent advancements in microscale acoustic streaming, including high-frequency transducer-induced high-speed streaming, confinement and programed streaming, and acoustic streaming tweezers, which combine acoustic radiation force and drag force to overcome size limitations in conventional acoustic manipulations. The article also provides a brief overview of acoustic streaming theory, its generation, applications, and key issues in the field. Lastly, the future prospects of micro acoustic streaming in bioanalytical and biomedical applications are discussed.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Manufacturing
Yancheng Wang, Chengyao Xu, Jiawei Liu, Hemin Pan, Yang Li, Deqing Mei
Summary: The acoustic-assisted 3D printing method enables the patterning of functional materials into diverse forms with high spatial resolutions, making it possible to fabricate complex functional composites with customized properties.
ADDITIVE MANUFACTURING
(2022)
Article
Chemistry, Multidisciplinary
Zhiyuan Chen, Jiuling Liu, Minghua Liu, Ran You, Shitang He
Summary: This research proposed and experimented a SAWR-based multi-sized particles monitor, demonstrating good linearity and repeatability in response to large coarse particles, inhalable particles, and fine inhalable particles.
APPLIED SCIENCES-BASEL
(2021)
Article
Acoustics
Mushtaq Ali, Jinsoo Park
Summary: In droplet-based microfluidic platforms, there is increasing demand for precise separation of microscale droplets of different chemical composition. Most existing techniques focus on separating droplets of the same kind, while there is less effort on separating uniform-sized droplets of different chemical composition. This study proposes an acoustofluidic method using ultrasonic surface acoustic wave (SAW)-induced acoustic radiation force (ARF) to simultaneously separate uniform-sized droplets of different acoustic impedance. The experimental results show that the proposed method provides a label-free and detection-free approach for on-chip, precise separation of multiple kinds of droplets in microfluidic applications.
ULTRASONICS SONOCHEMISTRY
(2023)
Article
Engineering, Electrical & Electronic
Jun Long Han, Hong Hu, Qing Yun Huang, Yu Lin Lei
Summary: In this study, an acoustofluidic platform based on standing surface acoustic waves (SSAWs) was established to analyze the influence of droplet contact angle on particle separation. Experimental results demonstrated the importance of reducing the contact angle for achieving better particle separation efficiency.
SENSORS AND ACTUATORS A-PHYSICAL
(2021)
Article
Energy & Fuels
H. M. D. P. Herath, M. A. Wijewardane, R. A. C. P. Ranasinghe
Summary: In recent years, there has been a growing interest in new power generation technologies and thermal energy recovery due to environmental and economic concerns. Thermo-acoustic generation, specifically traveling wave thermo-acoustic generators, has been identified as a promising technology for low-grade energy recovery and power generation. These generators have shown higher efficiency and simplicity compared to standing-wave thermoacoustic generators, making them a cost-effective and reliable option for energy conversion.
Article
Microscopy
Honglin Lv, Xueye Chen, Yaolong Zhang, Xiangyang Wang, Xiangwei Zeng, Dengying Zhang
Summary: This article proposes a two-stage particle separation channel using ultrasound for precise manipulation of micro and nano particles. Through numerical simulation and experimentation, the performance of different piezoelectric substrate materials was compared and optimal parameters were selected to achieve successful particle separation. This work provides a theoretical foundation for rapid disease diagnosis and real-time environmental monitoring.
JOURNAL OF MICROSCOPY
(2022)
Article
Chemistry, Analytical
Umar Farooq, Xiufang Liu, Wei Zhou, Muhammad Hassan, Lili Niu, Long Meng
Summary: This method utilizes the collision between nanowires and cells to induce cell lysis efficiently, with advantages of low power consumption, fast processing, reusability, and minimal sample consumption. Ni coated Ag-nanowires show promise for applications in point of care diagnostics.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Engineering, Multidisciplinary
Tao Peng, Mingyong Zhou, Shuai Yuan, Cui Fan, Bingyan Jiang
Summary: The tilted-angle standing surface acoustic wave (taSSAW) microfluidic device is a powerful tool for biosample separation, with the reliability of a numerical model being validated. Experimental validation of the numerical model's predictions, and analysis of various parameters affecting particle deflection, were conducted.
APPLIED MATHEMATICAL MODELLING
(2022)
Article
Nanoscience & Nanotechnology
Citsabehsan Devendran, David J. Collins, Adrian Neild
Summary: Surface acoustic wave (SAW) micromanipulation offers modularity, easy integration into microfluidic devices and a high degree of flexibility. However, reducing the threshold for manipulating smaller particles is a major challenge. The novel excitation configuration based on diffractive-acoustic SAW (DASAW) actuation shows promise in reducing the critical minimum particle size that can be manipulated, with the ability to tune the streaming magnitude by changing the channel height.
MICROFLUIDICS AND NANOFLUIDICS
(2022)
Article
Materials Science, Multidisciplinary
Jianwei Zhong, Peixian Li, Minhui Liang, Ye Ai
Summary: The importance of cryopreservation for long-term storage of primary cell samples is highlighted, with special emphasis on the challenges of low viability and cell fragmentation. A label-free viability assessment and on-demand enrichment system for cryopreserved primary human PBMCs with over 90% viability of sorted samples in one step after thawing is demonstrated. This integrated system has broad applications in diverse cellular biology research and cell-related biomedical diagnostics.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Article
Chemistry, Analytical
Xiaoguang Lu, Ye Ai
Summary: A centrifuge-free and automatic cell wash platform, Puriogen, has been developed for efficient cell purification and has broad applications in cell preparation.
ANALYTICAL CHEMISTRY
(2022)
Article
Engineering, Biomedical
Minhui Liang, Jianwei Zhong, Ye Ai
Summary: Cellular mechanical properties play a significant role in cell state and health. Microfluidic mechanical phenotyping methods are promising tools that can address the limitations of traditional approaches. This study comprehensively compares two types of microfluidic cellular mechanical phenotyping methods and provides important findings.
ADVANCED HEALTHCARE MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
William Sean Harley, Kirill Kolesnik, Mingxin Xu, Daniel Edward Heath, David John Collins
Summary: This work demonstrates a method to generate designed and highly localized acoustic fields using 3D resonant mass-spring microstructures, enabling rapid and spatially defined controllable micromanipulation. This sub-wavelength, 3D acoustofluidic approach has potential applications in sample preparation, cell analysis, and diagnostics.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Mingxin Xu, William S. Harley, Zhichao Ma, Peter V. S. Lee, David J. Collins
Summary: Acoustic metasurfaces with complex 3D structures complicate their fabrication and applicability to higher frequencies, but an ultrathin metasurface approach utilizing planarized micropillars in a discretized phase array is demonstrated here. This subwavelength metasurface can be easily produced via a single-step etching process and is suitable for megahertz-scale applications. The flexibility of this approach is further demonstrated in the production of complex acoustic patterns via acoustic holography. This metasurface approach, combined with predictive models, has broad potential for robust, high-frequency acoustic manipulation in various applications.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Analytical
Rasool Dezhkam, Hoseyn A. Amiri, David J. Collins, Morteza Miansari
Summary: This study performs a comprehensive investigation on the application of low-voltage ICP technology in extracting submicron particles. By establishing a mathematical model and conducting experiments, the researchers discover that the charges and sizes of particles determine their capture or release from ICP-induced vortices, with the primary determinant of particle trajectory being the surface charge of the particles.
Article
Biophysics
Minhui Liang, Qiang Tang, Jianwei Zhong, Ye Ai
Summary: Microfluidics provides a powerful platform for biological analysis by precisely manipulating fluids and microparticles. The imaging and impedance cell analyzer (IM2Cell) introduced here combines single cell level impedance analysis and hydrodynamic mechanical phenotyping, demonstrating multi-stress level mechanical phenotyping capabilities. IM2Cell can characterize cell diameter, deformability responses, and electrical properties, providing high-dimensional information about subcellular components. It has been validated for different cell lines and shows potential for deformability studies of PBMC subpopulations.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Physical
Jianwei Zhong, Minhui Liang, Ye Ai
Summary: Co-encapsulation of bead carriers and biological cells in microfluidics has become a powerful technique for various biological assays in single-cell genomics and drug screening. However, current co-encapsulation approaches limit the effective throughput due to a trade-off between cell/bead pairing rate and probability of multiple cells in individual droplets. The DUPLETS system is reported to overcome this problem by differentiating the encapsulated content in individual droplets and sorting out targeted droplets via a combined screening of mechanical and electrical characteristics.
Article
Engineering, Biomedical
Jianwei Zhong, Minhui Liang, Qiang Tang, Ye Ai
Summary: By introducing a label-free selectable cell quantity encapsulation in droplets sorting system, the purity and throughput of single-cell droplets can be improved. This system combines electrical impedance based screening with biocompatible acoustic sorting to achieve high efficiency and throughput while removing multi-cells and empty droplets.
MATERIALS TODAY BIO
(2023)
Article
Chemistry, Multidisciplinary
Jiahong Chen, Jianwei Zhong, Yifu Chang, Yinning Zhou, Seok Hwee Koo, Thean Yen Tan, Hongtao Lei, Ye Ai
Summary: Antimicrobial resistance is a serious threat to global public health. A label-free electrical impedance-based microfluidic platform has been designed to expedite and streamline antimicrobial susceptibility testing (AST) for clinical practice, with a rapid 2-minute AST assay at the single-bacterium level. This platform enables accurate analysis of bacterial viability and determination of antimicrobial resistance.
Article
Chemistry, Analytical
Kirill Kolesnik, Daniel Quang Le Pham, Jessica Fong, David John Collins
Summary: Researchers have found that a particle focusing spot in the center of a rotating fluid is formed by the secondary flow. The rotational velocity and the shape of the vessel significantly influence the particle's equilibrium position. This study demonstrates the formation of a single focusing spot in the center of the vessel and the repeatable formation of stable ring-shaped particle arrangements.
Article
Biochemical Research Methods
Kirill Kolesnik, Philipp Segeritz, Daniel J. Scott, Vijay Rajagopal, David J. Collins
Summary: Acoustofluidic devices can manipulate particles and cells in arbitrary configurations at the microscale without complex patterning. This approach is enabling for cell studies and tissue engineering applications.
Article
Biochemical Research Methods
Jiahong Chen, Jianwei Zhong, Hongtao Lei, Ye Ai
Summary: This study presents a novel microfluidic electrical impedance-based multidimensional single-bacterium profiling system that can detect bacteria in a wide concentration range and accurately differentiate their viability and gram types. The system utilizes multi-frequency impedance quantification to analyze the size, concentration, and membrane impedance of bacteria in a single flow-through interrogation. The system has been demonstrated to have a wide bacterial counting range and can rapidly and accurately discriminate the viability and gram types of bacteria in a label-free manner.
Article
Chemistry, Analytical
Xiaoguang Lu, Ye Ai
Summary: A centrifuge-free and automatic cell wash platform, Puriogen, has been developed in this study, which can efficiently remove interfering substances from cell samples, improve cell wash efficiency and cell viability, and has broad applications in cell preparation.
ANALYTICAL CHEMISTRY
(2022)