期刊
ANALYTICAL CHEMISTRY
卷 88, 期 20, 页码 10191-10197出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.6b02772
关键词
-
资金
- National Natural Science Foundation of China [21475048]
- National Science Fund for Distinguished Young Scholars of Guangdong Province [2014A030306008]
- Project of Guangzhou Science and Technology Plan [201508020003]
- Program of the Pearl River Young Talents of Science and Technology in Guangzhou [2013J2200021]
- Special Support Program of Guangdong Province [2014TQ01R599]
- Outstanding Young Teacher Training Program of Guangdong Province [HS2015004]
Genetic analysis. is of great importance for the detection of pathogenic bacteria. Bacterial identification must become simpler, less expensive, and more rapid than the traditional methods. In this study, a low-cost, label-free, and, wireless paper-based bipolar electrode electrochemiluminescence (pBPE-ECL) analysis system was constructed for the rapid and, sensitive genetic detection of pathogenic bacteria. Wax-screen printing was used to form hydrophilic channels on filter paper, and a carbon ink-based bipolar electrode and driving electrodes were screen-printed. into the channels, The light-switch molecule [Ru(phen)(2)dppz](2+) (phen = 1,10-phenanthroline; dppz = dipyridophenazine) was used to intercalate into the base pairs of the double-stranded DNA PCR amplification products, and the complexs were then applied to the paper-based bipolar electrode to-perform the ECL,assays; the ECL of [Ru(phen)(2)dppz](2+) is quenched in aqueous solution, but this molecule displays intense ECL when intercalated into double-stranded DNA. Under optimized, experimental conditions, as little as 10 copies/mu L of the genomic DNA of Listeria monocytogenes could be detected. Additionally) the system could also specifically distinguish Listeria monocytogenes. from Salmonella,. Escherichia coli O157:H7, and Staphylococcus aureus. This label-free, simple, and rapid method has potential in point-of-care applications for pathogen detection.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据