Article
Chemistry, Analytical
Xiaorong Zhang, Yuan Deng, Hongzhao Qiu, Sirui Yi, Sijia Huang, Lanlan Chen, Shanwen Hu
Summary: In this study, an electrochemiluminescence-based platform was proposed for sensitive detection of H. pylori DNA. The strategy demonstrated excellent stability and selectivity, with a low detection limit of 10 pM. Additionally, a biosensing chip was designed to improve throughput and enable large-scale clinical biosensing applications.
Article
Chemistry, Analytical
Wenyan Jiang, Zhongping Chen, Jin Lu, Xue Ren, Yue Ma
Summary: This study developed a new detection method based on gold nanoparticles and CRISPR/Cas12a for highly sensitive detection of miRNA-143 as a noninvasive biomarker for prostate cancer diagnosis. The method exhibited outstanding specificity and sensitivity, and it is simple to operate, making it suitable for use in low-resource settings.
Article
Chemistry, Analytical
Juan Song, Shan Li, Jie Zhou, Qiao Yu, Xue-Jiao Yang, Hong-Yuan Chen, Jing-Juan Xu
Summary: We have developed a triple signal amplification strategy for in situ visualization of telomerase RNA (TR) in living cells. The strategy includes target-initiated strand displacement circuit, multidirectional rolling circle amplification (RCA), and Mg2+ DNAzyme-mediated amplification. The proposed method exhibited ultrasensitive detection of TR mimic with a detection limit at the picomolar level. Moreover, after transfection with the proposed sensing platform, tumor cells can be easily distinguished from normal cells based on TR abundance-related fluorescence signal, providing new insights into initial cancer screening.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Na Wu, Hui-Chao Zhang, Xu-Hong Sun, Feng-Na Guo, Li-Xia Feng, Ting Yang, Jian-Hua Wang
Summary: By combining Exo III-assisted target recycling amplification with rolling circle amplification, a novel silver nanocluster was used as fluorescence probes to achieve ultrasensitive HIV/HCV DNA detection. Furthermore, through the design of padlock probes and utilization of generated ssDNA fragments, an intelligent DNA machine was constructed to distinguish multiple virus DNAs.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Multidisciplinary
Dingran Chang, Jiuxing Li, Rudi Liu, Meng Liu, Kha Tram, Natalie Schmitt, Yingfu Li
Summary: Here we report on an ultra-sensitive colorimetric sensing platform that combines the amplification power of rolling circle amplification (RCA) with the efficiency of a urease-mediated litmus test. By utilizing aptamers for target recognition, this platform can be adapted for a wide range of sensing applications.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Analytical
Ruolan Huang, Kun Liu, Chensirong Yue, Ying Wang, Zhilong Zeng, Lu Xiao, Lei Dong, Rongqin Ke, Chaoyong Yang, Dan Liu
Summary: This study developed a method for visualizing mRNA at single-molecule resolution using rolling circle amplification and streptavidin-modified alkaline phosphatase (SA-ALP) detection. The method provides highly selective and sensitive RNA detection, comparable to fluorescence-based assays. It also overcomes common background interference issues and allows for the quantification of mRNA expression from two genes in single cells and multiple cell types.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Jayeon Song, Yong Ju, Soohyun Kim, Hansol Kim, Hyun Gyu Park
Summary: In this study, we developed a palindromic hyperbranched rolling circle amplification (PH-RCA) reaction for ultrasensitive detection of microRNAs. The PH-RCA reaction significantly enhances the sensitivity and reduces the reaction time within 30 minutes, making it a promising technique for miRNA detection.
CHEMICAL COMMUNICATIONS
(2022)
Article
Multidisciplinary Sciences
Georgia Diakoudi, Paolo Capozza, Gianvito Lanave, Francesco Pellegrini, Barbara Di Martino, Gabriella Elia, Nicola Decaro, Michele Camero, Paola Ghergo, Fabio Stasi, Alessandra Cavalli, Maria Tempesta, Vanessa R. Barrs, Julia Beatty, Krisztian Banyai, Cristiana Catella, Maria S. Lucente, Alessio Buonavoglia, Giovanna Fusco, Vito Martella
Summary: Hepadnaviruses can be found in dogs, similar to the hepadnavirus in domestic cats. Canine hepadnavirus can cause altered hepatic markers, but its role in canine health remains unclear.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Analytical
Yu Gu, Xuejiao Yang, Shanwen Hu, Chunxian Guo, Bo Chen, Cunxia Fan, Chang Ming Li
Summary: A novel glucometer-based microfluidic platform was developed for sensitively detecting cancer biomarker HER2. The assay uses rolling cycle amplification technology with a miniaturized personal glucose meter, on a small-sized microfluidic chip with high sensitivity and wide dynamic range, showing great potential for clinical diagnosis at home or in urban areas.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Linying Yu, Liping Zhu, Yao Peng, Mengting Sheng, Jianshe Huang, Xiurong Yang
Summary: A highly sensitive and versatile dual-amplification electrochemiluminescence (ECL) biosensing platform was developed for rapid and sensitive detection of biomarkers. The platform utilized DNA nanostructures and catalyzed hairpin assembly (CHA) to achieve high amplification efficiency. The biosensor demonstrated a detection limit of 1.09 fM for target DNA and showed selective detection of silver ions. The proposed strategy has potential applications in disease diagnosis and environmental monitoring.
ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Lulu Xu, Jiaxin Duan, Junman Chen, Shijia Ding, Wei Cheng
Summary: RCA is an efficient enzymatic isothermal reaction using circular probes to amplify circular templates rapidly, with high specificity and the ability to generate customized functional nucleic acids for in situ detection.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Shuang Xia, Wenli Wu, Fangchao Yin, Jianguo Shi, Yaohong Ma, Jin-Ming Lin, Xu Wu, Qiongzheng Hu
Summary: A smartphone-based colorimetric assay is developed in this study, which utilizes the aggregation of gold nanoparticles (AuNPs) and rolling circle amplification (RCA) on magnetic beads (MBs) for the detection of adenosine. By utilizing specific aptamer recognition, in-situ RCA on MBs, magnetic separation, and smartphone-based portable device, this assay achieves a low limit of detection (LOD) for adenosine of 16 pM and works well in human serum.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biochemistry & Molecular Biology
Xianming Jiang, Xiaoguang Pan, Wenchao Li, Peng Han, Jiaying Yu, Jing Li, Haoran Zhang, Wei Lv, Ying Zhang, Yulong He, Xi Xiang
Summary: This study found that the abundance of eccDNA was significantly higher in gastric cancer tissues compared to normal tissues. The eccDNA carrying oncogene-segments may contribute to the development of gastric cancer and facilitate cancer progression. Blocking the pathways for eccDNA generation could be a potential therapeutic strategy for gastric cancer.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Yu Wang, Wenyu Sun, Jingfeng Wang, Xu Wang, Yicheng Xu, Yuanzhen Guo, Yeru Wang, Manru Zhang, Long Jiang, Su Liu, Jiadong Huang
Summary: A facile and robust exponential rolling circle amplification (RCA) strategy has been proposed for sensitive biosensing, utilizing primer-remodeling amplification with a repair enzyme and polymerase for detecting UDG activity. This approach shows potential for ultrasensitive detection of low levels of analytes in clinical diagnosis and fundamental biomedicine research.
Article
Chemistry, Analytical
Xiaoyue Zhang, Xueying Zhu, Yuanfang Li, Xin Hai, Sai Bi
Summary: In this study, a colorimetric/photothermal dual-mode exosomes sensing platform was developed for the detection of human breast cancer cell (MCF-7)-derived exosomes. The platform utilizes the encapsulation of 3,3',5,5'-tetramethylbenzidine-loaded graphene quantum dot nanozymes (TMB-GQDzymes) into DNA flowers (DFs) via rolling circle amplification (RCA). The dual-aptamer recognition strategy enables specific detection, and the platform showed excellent performance in distinguishing breast cancer patients from healthy individuals in serum samples analysis.