期刊
NATURE
卷 566, 期 7742, 页码 94-+出版社
NATURE PORTFOLIO
DOI: 10.1038/s41586-019-0894-z
关键词
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资金
- National Institutes of Health [DP2 GM105434, GM119707]
- Cottrell Scholar Award
- Camille Dreyfus Teacher-Scholar Award
- Searle Scholars Program
- Harvard University
- National Cancer Institute [ACB-12002]
- National Institute of General Medical Sciences [AGM-12006]
- DOE Office of Science [DE-AC02-06CH11357]
- NIH-Office of Research Infrastructure Programs, High-End Instrumentation Grant [1S10OD012289-01A1]
- Michigan Economic Development Corporation
- Michigan Technology Tri-Corridor [085P1000817]
Small molecules containing the N-nitroso group, such as the bacterial natural product streptozotocin, are prominent carcinogens(1,2) and important cancer chemotherapeutics(3,4). Despite the considerable importance of this functional group to human health, enzymes dedicated to the assembly of the N-nitroso unit have not been identified. Here we show that SznF, a metalloenzyme from the biosynthesis of streptozotocin, catalyses an oxidative rearrangement of the guanidine group of N.-methyl-l-arginine to generate an N-nitrosourea product. Structural characterization and mutagenesis of SznF reveal two separate active sites that promote distinct steps in this transformation using different iron-containing metallocofactors. This biosynthetic reaction, which has little precedent in enzymology or organic synthesis, expands the catalytic capabilities of non-haem-iron-dependent enzymes to include N-N bond formation. We find that biosynthetic gene clusters that encode SznF homologues are widely distributed among bacteria-including environmental organisms, plant symbionts and human pathogenswhich suggests an unexpectedly diverse and uncharacterized microbial reservoir of bioactive N-nitroso metabolites.
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