Article
Peripheral Vascular Disease
Saroj Chakraborty, Anju Lulla, Xi Cheng, Ji-Youn Yeo, Juthika Mandal, Tao Yang, Xue Mei, Piu Saha, Rachel M. Golonka, Beng San Yeoh, Blair Mell, Wei Jia, Vasanta Putluri, Danthasinghe Waduge Badrajee Piyarathna, Nagireddy Putluri, Arun Sreekumar, Katie Meyer, Matam Vijay-Kumar, Bina Joe
Summary: Hypertension is a major risk factor for global mortality, and targeting the gut-liver axis may provide a new avenue for developing antihypertensive therapeutics. In this study, the researchers found that conjugated bile acids were inversely correlated with blood pressure in humans and rats. Intervention with taurine or tauro-cholic acid rescued bile acid conjugation and reduced blood pressure in hypertensive rats. This work reveals conjugated bile acids as nutritionally re-programmable anti-hypertensive metabolites.
JOURNAL OF HYPERTENSION
(2023)
Article
Nutrition & Dietetics
Yutao Chen, Jun Lu, Kristin Wickens, Thorsten Stanley, Robyn Maude, Peter Stone, Christine Barthow, Julian Crane, Edwin A. Mitchell, Fabrice Merien, Rinki Murphy
Summary: The study demonstrated that probiotic supplementation of HN001 can decrease the incidence of gestational diabetes mellitus and fasting glucose levels. It also found associations between probiotic supplementation and alterations in insulin resistance, lipid levels, and bile acids in pregnant women.
Article
Chemistry, Analytical
Armaghan Shafaei, Joanna Rees, Claus T. Christophersen, Amanda Devine, David Broadhurst, Mary C. Boyce
Summary: This study presents a simple extraction method for the analysis of bile acids in feces, followed by quantification using liquid chromatography and tandem mass spectrometry. The method shows good precision and accuracy, making it suitable for large-scale analysis of fecal samples.
ANALYTICA CHIMICA ACTA
(2021)
Article
Endocrinology & Metabolism
Carl Jenkinson, Reena Desai, Malcolm D. McLeod, Jonathan Wolf Mueller, Martin Hewison, David J. Handelsman
Summary: This study analyzed the contribution of sulfate and glucuronide vitamin D metabolites relative to unconjugated levels in human serum. The results showed that sulfate conjugates formed a higher proportion of circulating vitamin D metabolites compared to glucuronide conjugates. The findings suggest that a combination of both conjugated and unconjugated measurements may provide a more accurate assessment of vitamin D status.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2022)
Article
Endocrinology & Metabolism
Alison L. Dickson, Eylan Yutuc, Catherine A. Thornton, Yuqin Wang, William J. Griffiths
Summary: The aim of this study was to identify oxysterols and their downstream metabolites in various biological fluids during pregnancy. The researchers confirmed the presence of 20S-hydroxycholesterol in human placenta and proposed a metabolic pathway from 22R-hydroxycholesterol to a C-27 bile acid. This pathway was observed not only in placenta but also in umbilical cord blood plasma, maternal plasma, and amniotic fluid, indicating its importance in pregnancy.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biochemical Research Methods
Martin Uher, Stanislav Micuda, Marian Kacerovsky, Milos Hroch
Summary: Despite the challenges posed by the natural presence of endogenous substances in a biological matrix, this study proposed an alternative approach using authentic analytical standards to prepare validation reference samples. The modified method based on standard-addition procedure showed advantages over other commonly used methods in LC-MS/MS analysis, and was successfully validated with low limit of quantification and good linearity.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Toxicology
Martina Franzin, Rachele Ruoso, Michela Peruch, Gabriele Stocco, Stefano D'Errico, Riccardo Addobbati
Summary: Sudden death can occur in adults due to illicit drug use for recreational purposes or intoxication in children, and toxicological testing can help determine the cause of death. A LC-MS/MS method was proposed in this study to quantify 108 drugs and metabolites in bile, which could serve as an alternative specimen for toxicological testing. Methadone and cocaine were found to be the most prevalent substances consumed, and bile showed higher concentrations of drugs compared to blood, indicating its potential as a new matrix for identifying illicit drugs and their metabolites.
ARCHIVES OF TOXICOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Shin Koike, Yusuke Miyaji, Kazuhiro Suzuki, Mitsuhiro Miyashita, Masanari Itokawa, Makoto Arai, Yuki Ogasawara
Summary: This study measured bile acids in plasma from patients with schizophrenia and healthy subjects and found significant differences in unconjugated bile acid levels between the two groups. The results suggest that plasma bile acid levels may serve as diagnostic markers for schizophrenia.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Ambrin Farizah Babu, Ville Mikael Koistinen, Soile Turunen, Gloria Solano-Aguilar, Joseph F. Urban Jr, Iman Zarei, Kati Hanhineva
Summary: In this study, a sensitive and simple qualitative LC-MS/MS method was developed for the identification and distribution of sterols, bile acids, and acylcarnitines in biological samples. The method allowed the simultaneous detection and annotation of these metabolites from reference standards and different biological samples, providing a valuable tool for screening these metabolites in routine scientific research.
Article
Nutrition & Dietetics
William Gagnon, Veronique Garneau, Jocelyn Trottier, Melanie Verreault, Charles Couillard, Denis Roy, Andre Marette, Jean-Philippe Drouin-Chartier, Marie-Claude Vohl, Olivier Barbier
Summary: This study analyzed the impact of dietary supplementation with blueberry powder on the composition of fecal bile acids (BA). The results showed that the consumption of blueberry powder led to significant increases in glycine-conjugated BAs, glycochenodeoxycholic acid, and glycoursodeoxycholic acid, as well as a significant reduction in secondary BA levels.
Article
Endocrinology & Metabolism
Chen Pan, Dawei Deng, Tianfu Wei, Zeming Wu, Biao Zhang, Qihang Yuan, Guogang Liang, Yanfeng Liu, Peiyuan Yin
Summary: Bile acids are potential biomarkers for the early diagnosis of gastric cancer. A diagnostic panel comprising six bile acids or ratios shows high accuracy in diagnosing gastric cancer.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ryo Konno, Takashi Matsui, Hiroaki Ito, Yusuke Kawashima, Makoto Itakura, Yoshio Kodera
Summary: GeLC-MS/MS method coupled with stable isotope dimethyl labeling provides high accuracy and comprehensive peptide comparisons, enabling accurate determination of peptide abundance ratio between samples, unaffected by the reproducibility of gel extraction or LC-MS procedures, thus facilitating the detection of proteolysis events.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Yuan-Chih Chen, Hsin-Yi Wu, Chih-Wei Chang, Pao-Chi Liao
Summary: Conjugation reactions play a critical role in human metabolism, but identifying conjugated metabolites is challenging. In this study, a strategy called PDMS2E-DIA was proposed for comprehensive profiling of the glucuronide-conjugated metabolome. It successfully identified a large number of glucuronide-conjugated metabolites in human urine samples.
ANALYTICAL CHEMISTRY
(2022)
Article
Environmental Sciences
Denis A. M. da Silva, Jonelle B. Gates, Sandra M. O'Neill, James E. West, Gina M. Ylitalo
Summary: Polycyclic aromatic hydrocarbons (PAHs) are common environmental contaminants monitored in marine biota. Fish can metabolize PAHs into hydroxylated forms (OHPAHs) which may be more toxic. Traditional monitoring of parent PAHs in fish can lead to underestimation of PAH exposure.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Agriculture, Multidisciplinary
Sinah Reiter, Andreas Dunkel, Corinna Dawid, Thomas Hofmann
Summary: Bile acids are increasingly investigated as markers for various diseases in humans and animals. A high-throughput workflow was used for bile acid analysis in animal studies, mapping complex bile acid profiles in tissues and body fluids. This method can also be used for screening food and studying the bioavailability of bile acids in the GI tract.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Gastroenterology & Hepatology
Yuhan Yin, Anna Sichler, Josef Ecker, Melanie Laschinger, Gerhard Liebisch, Marcus Hoering, Marijana Basic, Andre Bleich, Xue-Jun Zhang, Ludwig Kuebelsbeck, Johannes Plagge, Emely Scherer, Dirk Wohlleber, Jianye Wang, Yang Wang, Marcella Steffani, Pavel Stupakov, Yasmin Gaertner, Fabian Lohoefer, Carolin Mogler, Helmut Friess, Daniel Hartmann, Bernhard Holzmann, Norbert Hueser, Klaus-Peter Janssen
Summary: Hepatocyte growth and proliferation depend on membrane phospholipid biosynthesis, which is significantly influenced by short-chain fatty acids (SCFAs) generated through bacterial fermentation. Antibiotic treatment and dysbiosis not only affect gut microbiota, but also impair hepatic lipid synthesis and liver regeneration.
JOURNAL OF HEPATOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Nikolaos Pagonas, Felix S. Seibert, Gerhard Liebisch, Maximillian Seidel, Theodoros Giannakopoulos, Benjamin Sasko, Oliver Ritter, Nina Babel, Timm H. Westhoff
Summary: This study found an association between low levels of propionate, a short-chain fatty acid, and coronary artery disease (CAD).
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2023)
Article
Agriculture, Dairy & Animal Science
Lea Schaefer, Sarah M. Grundmann, Silvia Friedrichs, Dieter Luetjohann, Marcus Hoering, Gerhard Liebisch, Erika Most, Robert Ringseis, Klaus Eder
Summary: Replacing soybean oil with Hermetia illucen larvae fat alters the fatty acid composition of meat and reduces lipid oxidation.
ARCHIVES OF ANIMAL NUTRITION
(2023)
Article
Hematology
Lilli Arndt, Ileana Hernandez-Resendiz, Doreen Moos, Janine Dokas, Silvana Mueller, Franziska Jeromin, Richard Wagner, Uta Ceglarek, Iris M. Heid, Marcus Hoering, Gerhard Liebisch, Sonja C. Stadler, Ralph Burkhardt
Summary: This study demonstrates that deficiency of Trib1 promotes the formation of atherosclerotic lesions and increases plasma cholesterol and triglyceride levels in Ldlr(-/-) mice. It also suggests that Trib1 deficiency alters hepatic lipid metabolism and induces inflammation.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2023)
Article
Genetics & Heredity
Lisa Rein-Fischboeck, Rebekka Pohl, Elisabeth M. Haberl, Wolfgang Mages, Philipp Girke, Gerhard Liebisch, Sabrina Krautbauer, Christa Buechler
Summary: Visceral adiposity is associated with liver steatosis and the development of NASH. Mice lacking SNTB2 have reduced intra-abdominal fat mass and do not accumulate hepatic cholesteryl esters on a MCD diet. However, they still develop a similar NASH pathology as wild type mice, suggesting a minor role of intra-abdominal fat and liver cholesteryl esters in this model.
Article
Biochemistry & Molecular Biology
Georg Peschel, Kilian Weigand, Jonathan Grimm, Martina Mueller, Sabrina Krautbauer, Marcus Hoering, Gerhard Liebisch, Christa Buechler
Summary: This study analyzed the composition of sphingomyelin (SM) in the serum of patients with chronic hepatitis C virus (HCV) infection, and found differences in SM species among different genders, genotypes, and patients with liver cirrhosis. The levels of SM were more closely associated with liver function in male patients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Qingqing Wang, Miriam Hoene, Chunxiu Hu, Louise Fritsche, Robert Ahrends, Gerhard Liebisch, Kim Ekroos, Andreas Fritsche, Andreas L. Birkenfeld, Xinyu Liu, Xinjie Zhao, Qi Li, Benzhe Su, Andreas Peter, Guowang Xu, Rainer Lehmann
Summary: Reliability, robustness, and interlaboratory comparability of quantitative measurements in clinical lipidomics studies are crucial. The different ex vivo stability of lipids in blood poses a risk to data interpretation. Clear recommendations for blood sample collection are necessary. In this study, we used UHPLC-high resolution mass spectrometry to investigate the stability of 417 lipid species in EDTA whole blood under different temperature conditions and time points. Our data suggest that cooling whole blood immediately and continuously, and separating plasma within 4 hours, unless focusing on robust lipids, are recommended. Lists of lipids' ex vivo (in)stability and potential biomarkers in whole blood are provided. Overall, our results contribute to the international efforts in achieving reliable and comparable clinical lipidomics studies in the future.
JOURNAL OF LIPID RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Coenraad Frederik Slabber, Marc Bachofner, Tobias Speicher, Andrii Kuklin, Abbie Elisabeth Fearon, Susagna Padrissa-Altes, Roman Bogorad, Carla Horvath Rudigier, Daria Wust, Sabrina Krautbauer, Marcus Hoering, Gerhard Liebisch, Daniel G. Anderson, Christian Wolfrum, Ulrich Auf Dem Keller, Sabine Werner
Summary: Mice lacking FGF receptors 1 and 2 (Fgfr1 and Fgfr2) in hepatocytes are hypersensitive to cytotoxic injury during liver regeneration, which is associated with the protective role of the urinary-reproductive factor Uhrf2 in liver cells. Uhrf2 expression increases during regeneration after partial hepatectomy in an FGFR-dependent manner, but the abundance of Uhrf2 in liver cells is lower in FGFR-deficient mice. Hepatocyte-specific knockout of Uhrf2 leads to liver necrosis, impaired hepatocyte proliferation, and liver failure.
Article
Multidisciplinary Sciences
Susanne Heimerl, Marcus Hoering, Dominik Kopczynski, Alexander Sigruener, Christina Hart, Ralph Burkhardt, Anne Black, Robert Ahrends, Gerhard Liebisch
Summary: This study presents a standardized protocol for quantifying lipid species concentrations in basal and activated human platelets using flow-injection mass spectrometry. The results show changes in lipid species concentrations after platelet activation and the release of complex lipids. These concentrations provide important insights into the physiological role of platelet lipids and serve as a basis for studying the platelet lipidome in patients with hemorrhagic or thrombotic disorders.
SCIENTIFIC REPORTS
(2023)
Article
Agriculture, Dairy & Animal Science
Lea Schaefer, Sarah M. Grundmann, Garima Maheshwari, Marcus Hoering, Gerhard Liebisch, Erika Most, Klaus Eder, Robert Ringseis
Summary: This study comprehensively investigated the effect of using insect fat from Hermetia illucens larvae as a fat source in broiler diets. The results showed that replacing soybean oil with insect fat had no effect on the growth performance and metabolic health of broilers, suggesting that insect fat can be a sustainable alternative fat source in broiler diets.
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Analytical
Dominik Kopczynski, Nils Hoffmann, Nina Troppmair, Cristina Coman, Kim Ekroos, Michael R. Kreutz, Gerhard Liebisch, Dominik Schwudke, Robert Ahrends
Summary: Lipid analysis is important for understanding the various functions of lipids, and LipidSpace is a standalone tool that analyzes lipidomes by assessing their structural and quantitative differences. It offers a user-friendly GUI and support for multiple data formats, allowing for reanalysis and merging of datasets, and provides additional discoveries.
ANALYTICAL CHEMISTRY
(2023)
Article
Pharmacology & Pharmacy
Ranjithkumar Rajendran, Vinothkumar Rajendran, Gregor Boettiger, Christine Stadelmann, Kian Shirvanchi, Laureen von Au, Sudhanshu Bhushan, Natascha Wallendszus, Darja Schunin, Victor Westbrock, Gerhard Liebisch, Sueleyman Erguen, Srikanth Karnati, Martin Berghoff
Summary: This study demonstrates the therapeutic potential of the FGFR inhibitor infigratinib in preventing and suppressing the first clinical episodes of MOG(35-55)-induced experimental autoimmune encephalomyelitis, reducing inflammation and neurodegeneration. Infigratinib also promotes oligodendrocyte maturation and remyelination, and decreases neurodegeneration-associated lipids. Therefore, infigratinib holds promise as a treatment for multiple sclerosis.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Article
Nutrition & Dietetics
Sarah M. Grundmann, Kerstin Ress, Lea Zimmermann, Marcus Hoering, Gerhard Liebisch, Erika Most, Robert Ringseis, Klaus Eder
Summary: A high phosphorus intake is associated with metabolic disorders and this study investigated the impact of a high-phosphorus diet on mouse skeletal muscle lipid composition and gene transcription.
Article
Cell Biology
Thomas Timm, Christiane Hild, Gerhard Liebisch, Markus Rickert, Guenter Lochnit, Juergen Steinmeyer
Summary: The levels of lysophosphatidylcholine (LPC) are elevated in the synovial fluid of patients with osteoarthritis (OA). Different LPC species have varying effects on the regulation of proteins and phospholipids in fibroblast-like synoviocytes (FLSs). IL-1 & beta; has the most significant impact on the regulation of proteins by LPC 16:0, suggesting its potential role in modulating inflammatory and catabolic mechanisms.
Article
Biology
Sabrina Ehnert, Anna J. Schreiner, Claudine Seeliger, Josef Ecker, Fabian Springer, Gerhard Liebisch, Philipp Hemmann, Tina Histing, Andreas K. Nussler
Summary: Using fatty acid methyl ester gas chromatography-mass spectrometry, this study found that both saturated and unsaturated fatty acids increase in the bone marrow. Characteristic fatty acid patterns were identified in patients with normal BMD, osteopenia, and osteoporosis, and different patterns were found in plasma, red bone marrow, and yellow bone marrow. Specific fatty acids correlated with osteoclast activity and suggested a possible mechanism for how fatty acids interfere with BMD, but no single fatty acid in the profile could be claimed for controlling BMD.