Article
Chemistry, Multidisciplinary
Runli Liang, Guangzhong Ma, Wenwen Jing, Yan Wang, Yunze Yang, Nongjian Tao, Shaopeng Wang
Summary: A charge-sensitive optical detection (CSOD) technique has been developed to detect molecular binding kinetics in normal ionic strength buffers, overcoming the challenge of decreased sensitivity in mass-based detection technologies for small molecules. By designing an H-shaped sample well and using agarose gels to cover electrodes, the study successfully observed stronger affinities between G-protein-coupled receptors and their small molecule ligands in normal buffer compared to diluted buffer, potentially due to stronger electrostatic repulsion forces in diluted buffer.
Article
Chemistry, Analytical
Runli Liang, Yingnan Zhang, Guangzhong Ma, Shaopeng Wang
Summary: Phage display technology is a useful tool in peptide drug development, but the synthesis of peptides is costly and time-consuming for the accurate measurement of binding kinetics. A sensitive technology is needed to directly measure the binding kinetics of peptides on phages, reducing time and cost.
Article
Chemistry, Multidisciplinary
Hao Zhu, Zixuan Chen, Yun Chen, Jun-Jie Zhu
Summary: Quantifying the binding kinetics and affinities of protein-small molecule interactions is critical for biomarker validation, drug discovery, and deep understanding of various biological processes at the molecular-scale. A novel optical imaging platform based on MoS2 material is developed to measure the binding kinetics of protein-small molecules and protein-ions. The method is capable of distinguishing different inhibitors binding to different forms of kinase, and can be used for drug evaluation and mechanism exploration.
Article
Polymer Science
Ruslan Shakurov, Svetlana Sizova, Stepan Dudik, Anna Serkina, Mark Bazhutov, Viktorija Stanaityte, Petr Tulyagin, Valery Konopsky, Elena Alieva, Sergey Sekatskii, Julia Bespyatykh, Dmitry Basmanov
Summary: We propose and demonstrate dendrimer-based coatings that enhance the sorption of small molecules and sensitivity of a label-free biosensor. Our results show that the PAMAM-modified chip has significantly higher sorption efficiency compared to other materials. This dendrimer-based biosensor method has the potential to be an advanced tool for detecting biomolecule interactions.
Article
Biochemical Research Methods
Kiersten D. Lenz, Katja E. Klosterman, Harshini Mukundan, Jessica Z. Kubicek-Sutherland
Summary: Enzyme-linked immunosorbent assays (ELISAs) are widely used for protein target detection due to their ease of use, sensitivity, and ability to analyze large quantities of samples. However, detecting non-protein targets with ELISAs can be challenging due to the physical properties of these molecules. In this study, a unique lipoprotein capture ELISA was developed to detect non-protein biomarkers using the natural association between lipoproteins and amphiphilic molecules.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Chemistry, Analytical
Na Li, Minhui Li, Mei Li
Summary: A programmable catalytic molecular nanomachine based on cross-linked catalytic hairpin assembly (CCHA) reaction was constructed for highly sensitive detection of proteins and small molecules. This nanomachine provides a versatile platform for disease diagnosis and therapeutic drug monitoring by replacing recognition elements.
Article
Chemistry, Multidisciplinary
Kenji Hayamizu, Kota Koike, Kosuke Dodo, Miwako Asanuma, Hiromichi Egami, Mikiko Sodeoka
Summary: Palladium enolates derived from β-ketocarbonyl compounds are stable in air and moisture. This stability was utilized to develop a peptide purification system using β-ketoamide as a small affinity tag in aqueous media. A solid-supported palladium complex efficiently captured and released β-ketoamide-tagged molecules, allowing for the purification and identification of ligand-binding sites in proteins.
Article
Chemistry, Medicinal
Khuchtumur Bum-Erdene, I-Ju Yeh, Giovanni Gonzalez-Gutierrez, Mona K. Ghozayel, Karen Pollok, Samy O. Meroueh
Summary: This study reports small molecules that can bind to TEAD and inhibit its binding to YAP/TAZ, thereby suppressing tumor growth and metastasis.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Bo Yao, Yunze Yang, Nanxi Yu, Nongjian Tao, Di Wang, Shaopeng Wang, Fenni Zhang
Summary: This article introduces an optical imaging method to measure molecular interactions with live cells by tracking nanometer membrane fluctuations. The elastic properties of the cell are determined through analysis of the data, as well as the binding kinetics of glycoprotein to lectins. The method is anticipated to contribute to the understanding of cell interactions, mechanical assessment of diseases like cancer, and screening of membrane protein targeting drugs.
Article
Chemistry, Analytical
Ping Ouyang, Chenxin Fang, Jialun Han, Jingjing Zhang, Yuxing Yang, Yang Qing, Yubing Chen, Wenyan Shang, Jie Du
Summary: The study introduces an electrochemical DNA biosensor for protein detection based on DNA self-assembly and terminal protecting effects of small-molecule-linked DNA. This strategy allows for qualitative and quantitative analysis of target proteins with high sensitivity in complex samples.
Article
Chemistry, Analytical
Ge Song, Hongzhao Sun, Jian Chen, Zhigang Chen, Boyang Liu, Zhenghui Liu, Shan Cong, Zhigang Zhao
Summary: There is great research interest in building semiconductor-based SERS platforms, and this study demonstrates the use of MoO3·xH2O quantum dots to achieve highly efficient SERS sensing of small inorganic molecules. The study highlights the crucial role of the quantum size effect in enhancing SERS activity in semiconductor-based materials.
ANALYTICAL CHEMISTRY
(2022)
Article
Optics
Tobias Pfeiffer, Jens Klier, Georg von Freymann, Daniel Molter
Summary: Nonlinear frequency conversion provides an elegant method for detecting low-energy photons with different wavelengths from the pump wavelength while providing phase information. We propose a phase-sensitive measurement method in the terahertz spectral range using visible light, which enables fast determination of layer thicknesses and achieves phase-resolved detection of terahertz pulses with standard equipment.
Article
Optics
Oumeng Zhang, Zijian Guo, Yuanyuan He, Tingting Wu, Michael D. Vahey, Matthew D. Lew
Summary: Single-molecule orientation-localization microscopy is a powerful tool for studying biochemical processes, but the limited photon budget poses challenges for high-dimensional imaging. A raMVR microscope has been developed with isotropic 3D spatial resolution and precision in measuring molecular orientations, enabling detailed imaging of molecular dynamics within biological and chemical systems.
Article
Chemistry, Analytical
Ge Song, Hongzhao Sun, Jian Chen, Zhigang Chen, Boyang Liu, Zhenghui Liu, Shan Cong, Zhigang Zhao
Summary: There is a strong research interest in constructing highly effective semiconductor-based SERS platforms, but current tuning methods have not been successful in creating sensors for small inorganic molecules. This study demonstrates the use of MoO3 middotxH2O quantum dots for sensitive detection of hydrazine and other probe molecules, utilizing the quantum size effect to enhance SERS activity.
ANALYTICAL CHEMISTRY
(2022)
Article
Nanoscience & Nanotechnology
Pablo Martin-Baniandres, Wei-Hsuan Lan, Stephanie Board, Mercedes Romero-Ruiz, Sergi Garcia-Manyes, Yujia Qing, Hagan Bayley
Summary: Electro-osmosis in an anion-selective alpha-hemolysin nanopore is utilized for capturing, unfolding, and transporting polypeptides with more than 1,200 residues. By monitoring the ionic current at a single-molecule resolution, this method enables the mapping of post-translational modifications in polypeptide chains. The application of nanopore technology holds potential for identifying full-length proteoforms.
NATURE NANOTECHNOLOGY
(2023)
Article
Chemistry, Analytical
Hao Zhu, Guangzhong Ma, Zijian Wan, Hui Wang, Nongjian Tao
ANALYTICAL CHEMISTRY
(2020)
Article
Biochemical Research Methods
Pengfei Zhang, Guangzhong Ma, Wei Dong, Zijian Wan, Shaopeng Wang, Nongjian Tao
Article
Multidisciplinary Sciences
Guangzhong Ma, Zijian Wan, Yunze Yang, Pengfei Zhang, Shaopeng Wang, Nongjian Tao
NATURE COMMUNICATIONS
(2020)
Article
Chemistry, Multidisciplinary
Runli Liang, Guangzhong Ma, Wenwen Jing, Yan Wang, Yunze Yang, Nongjian Tao, Shaopeng Wang
Summary: A charge-sensitive optical detection (CSOD) technique has been developed to detect molecular binding kinetics in normal ionic strength buffers, overcoming the challenge of decreased sensitivity in mass-based detection technologies for small molecules. By designing an H-shaped sample well and using agarose gels to cover electrodes, the study successfully observed stronger affinities between G-protein-coupled receptors and their small molecule ligands in normal buffer compared to diluted buffer, potentially due to stronger electrostatic repulsion forces in diluted buffer.
Article
Chemistry, Multidisciplinary
Wenwen Jing, Yi Wang, Chao Chen, Fenni Zhang, Yunze Yang, Guangzhong Ma, Eric H. Yang, Christine L. N. Snozek, Nongjian Tao, Shaopeng Wang
Summary: The study introduces a gradient-based digital immunoassay method for rapid and sensitive detection of cardiac troponin T, requiring only a small plasma sample volume. Concentration gradient is quantified by counting gold nanoparticles bound to different test zones with optical imaging, achieving precise detection of biomarkers.
Article
Chemistry, Multidisciplinary
Pengfei Zhang, Guangzhong Ma, Zijian Wan, Shaopeng Wang
Summary: Single-molecule-based measurement can distinguish specific and nonspecific binding processes by quantifying the mass and binding dynamics of individual-bound analyte molecules, providing a convenient solution for high-resolution imaging on widely used prism-coupled SPR systems.
Article
Multidisciplinary Sciences
Guangzhong Ma, Runli Liang, Zijian Wan, Shaopeng Wang
Summary: CAR imaging is a novel method for quantifying molecular interactions on a glass surface, offering deeper sensing range and tunable sensitivity compared to SPR. It is also compatible with a broader spectrum of light and fluorescence.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Guangzhong Ma, Zijian Wan, Yunze Yang, Wenwen Jing, Shaopeng Wang
Summary: This study introduces a plasmonic imaging technique that enables precise 3D tracking of surface-tethered single particles in real time, with applications in studying DNA-enzyme interactions. The analysis of particle motion patterns allows for the identification of specific and nonspecific interactions in immunoassays, contributing to the understanding of molecular dynamics and interactions at the single-molecule level.
Article
Electrochemistry
Yunlei Zhao, Guangzhong Ma, Shaopeng Wang
Summary: This study presents a method for quantifying protein binding kinetics on nanoparticles without the need for elution and immobilization, by tracking changes in nanoparticle size upon ligand binding. The method simplifies the workflow while providing sufficient binding kinetics and affinity information for protein studies.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2022)
Article
Chemistry, Analytical
Pengfei Zhang, Rui Wang, Zijian Wan, Xinyu Zhou, Guangzhong Ma, Jayeeta Kolay, Jiapei Jiang, Shaopeng Wang
Summary: The article introduces a total internal reflection-based evanescent scattering microscopy technique that can detect single proteins without labeling and provides a high-precision measurement method for protein binding kinetics.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Runli Liang, Yingnan Zhang, Guangzhong Ma, Shaopeng Wang
Summary: Phage display technology is a useful tool in peptide drug development, but the synthesis of peptides is costly and time-consuming for the accurate measurement of binding kinetics. A sensitive technology is needed to directly measure the binding kinetics of peptides on phages, reducing time and cost.
Article
Chemistry, Multidisciplinary
Guangzhong Ma, Pengfei Zhang, Xinyu Zhou, Zijian Wan, Shaopeng Wang
Summary: This article presents a label-free single-molecule pulldown (LFSMP) technique that allows imaging of intracellular proteins and complexes with single-molecule sensitivity and low sample consumption. The technique is based on plasmonic scattering imaging, which enables direct imaging of captured molecules without labels and quantification of binding kinetics. The authors demonstrate the detection principle of LFSMP, study the phosphorylation of protein complexes involved in a signaling pathway, and investigate the use of kinetic analysis to improve pulldown specificity.
ACS CENTRAL SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Xiaoyan Zhou, Guangzhong Ma, Zijian Wan, Shaopeng Wang
Summary: Label-free techniques often struggle to measure small molecules or enzymatic processes due to their reliance on refractive index or mass change on the sensor surface. The combination of Surface Plasmon Resonance sensing with an Oscillating Biomolecule Layer (SPR-OBL) overcomes these limitations by enhancing sensitivity to mass, charge, and conformational changes, allowing for the detection of small molecules.
Article
Chemistry, Multidisciplinary
Zijian Wan, Guangzhong Ma, Pengfei Zhang, Shaopeng Wang
Summary: The article introduces a label-free, single-molecule technique based on the differences in size, charge, and antibody binding to identify different proteins. By measuring the oscillation of protein molecules, their size and charge can be determined simultaneously. The ability to separate and identify two different proteins in a mixture is expanded by studying the changes induced by varying buffer pH and antibody binding.
Article
Biology
Guangzhong Ma, Zijian Wan, Shaopeng Wang
Summary: Traditional protein analysis techniques like electrophoresis and Western blot rely on size and charge differences, while single-molecule techniques show potential in improving resolution to single-molecule level. The developed single-molecule approach in this study tether single proteins to a surface with a polymer linker, drive them into oscillation with an electric field, and track their response to determine both size and charge simultaneously. This innovative protocol aims to enable comprehensive single-protein analysis on a single platform.