Article
Biotechnology & Applied Microbiology
Mariya B. Sokol, Nikita G. Yabbarov, Mariia R. Mollaeva, Margarita Chirkina, Murad D. Mollaev, Artur Zabolotsky, Sergey L. Kuznetsov, Elena D. Nikolskaya
Summary: This study successfully increased the safety profile of paclitaxel by designing paclitaxel-loaded nanoparticles rAFP3d-NP. rAFP3d-NP showed high loading content and moderate size, with appropriate release rate and prolonged blood circulation. The study also found significant tumor growth inhibition by rAFP3d-NP.
Article
Biochemistry & Molecular Biology
Phuriwat Khiewkamrop, Chamraj Kaewraemruaen, Chonnavee Manipuntee, Chalathan Saengruengrit, Numpon Insin, Asada Leelahavanichkul, Warerat Kaewduangduen, Opor Sonpoung, Kasirapat Ariya-anandech, Nattiya Hirankarn, Patcharee Ritprajak
Summary: This study developed a new nanoparticle (NP) containing a biodegradable PDMAEMA-PLGA copolymer for target-oriented delivery to DCs in situ. PDMAEMA-PLGA NPs improved dexamethasone capability to convert BM-cDCs from an immunogenic to tolerogenic state, and Dex-NP therapy potentially alleviated lupus disease in Fcgr2b(-/-) mice by mediating Foxp3(+) Treg expansion in an antigen-specific manner. Findings from this study support the superior efficacy of DC-targeted therapy using the PDMAEMA-PLGA NP delivery system and its potential as a therapy for SLE.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Physical
Noe Escareno, Natalia Hassan, Marcelo J. Kogan, Josue Juarez, Antonio Topete, Adrian Daneri-Navarro
Summary: Nanoparticle-based drug delivery systems combined with high-affinity targeting ligands provide a sophisticated landscape in cancer theranostics. Antibodies, due to their high diversity and specificity, are extensively used to provide bioactivity to nanoparticulate systems. However, controlled assembly of nanoparticles with targeting ligands remains a challenge.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Chemistry, Medicinal
Mohd Zulhelmy Ahmad, Akmal Hidyat Bin Sabri, Qonita Kurnia Anjani, Juan Dominguez-Robles, Normala Abdul Latip, Khuriah Abdul Hamid
Summary: Intranasal delivery of levodopa using chitosan nanoparticles showed enhanced drug absorption in a rat model, with C2 formulation demonstrating optimal particle characteristics and encapsulation efficiency. This study suggests that formulating levodopa into chitosan nanoparticles for intranasal delivery could potentially improve drug bioavailability for Parkinson's disease treatment.
Article
Biophysics
Sara Gimondi, Joana Vieira de Castro, Rui L. Reis, Helena Ferreira, Nuno M. Neves
Summary: In this study, the interaction between nanoparticles (NPs) and different types of cells was investigated. The results showed that the internalization of NPs by cells was size-dependent, with the highest uptake efficiency observed for 30 nm NPs. Different endocytic pathways were initiated in the presence of particular NP sizes, suggesting the importance of size in mediating the interaction between NPs and specific cell types.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Engineering, Biomedical
Shiqing Ma, Shendan Xu, Minting Li, Yaqi Du, Guangjie Tian, Jiayin Deng, Wenyi Zhang, Pengfei Wei, Bo Zhao, Xuesong Zhang, Zihao Liu, Yonglan Wang
Summary: Restoring bone homeostasis is crucial for osteoporosis treatment. Short peptides with biological activity have gained attention, but their application is limited due to the lack of a stable and targeted delivery system. This study proposes a novel delivery system using AL-PLGA@P NPs to deliver osteogenic peptides, which has the ability to target bone and regulate bone homeostasis, providing advantages in osteoporosis treatment.
ADVANCED HEALTHCARE MATERIALS
(2023)
Review
Pharmacology & Pharmacy
Samrat Mazumdar, Deepak Chitkara, Anupama Mittal
Summary: The beneficial or deleterious effects of nanomedicines are influenced by their complex interactions with intracellular pathways and their subcellular fate. Manipulating the physicochemical attributes of nanocarriers can direct nanoparticles towards specific sub-cellular organelles, affecting cellular internalization and enhancing intracellular uptake via specific endocytic pathways.
ACTA PHARMACEUTICA SINICA B
(2021)
Article
Biotechnology & Applied Microbiology
Katherine Gonzalez, Gauri Gangapurwala, Julien Alex, Antje Vollrath, Zoltan Cseresnyes, Christine Weber, Justyna A. Czaplewska, Stephanie Hoeppener, Carl-Magnus Svensson, Thomas Orasch, Thorsten Heinekamp, Carlos Guerrero-Sanchez, Marc Thilo Figge, Ulrich S. Schubert, Axel A. Brakhage
Summary: The study investigated whether polymeric particles (PPs) can reach Aspergillus fumigatus conidia-containing phagolysosomes in macrophages. The results showed that PPs were efficiently taken up by macrophages and co-localized with conidia in the same phagolysosomes. Fusion of phagolysosomes containing PPs with phagolysosomes containing conidia was observed. This suggests that PPs can be used as a carrier system to target intracellular pathogens.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Maria Quiterio, Sandra Simoes, Andreia Ascenso, Manuela Carvalheiro, Ana Paula Leandro, Isabel Correia, Ana Silveira Viana, Pedro Faisca, Lia Ascensao, Jesus Molpeceres, Maria Manuela Gaspar, Catarina Pinto Reis
Summary: Insulin, a peptide hormone, plays an important role in wound healing. Research shows that insulin-loaded nanoparticles exhibit controlled release characteristics in vitro, and demonstrate good safety in cell experiments and preliminary animal assays.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Physical
Mahmoud Abdelkarim, Noura H. Abd Ellah, Mahmoud Elsabahy, Mohamed Abdelgawad, Sara A. Abouelmagd
Summary: This study explored the effect of channel geometry and design on poly (lactic-co-glycolic) acid (PLGA) NPs size and polydispersity index (PDI). It was found that changing channel length did not have a significant impact on particle size, but increasing diffusion area and reducing mixing time significantly reduced particle size. Introduction of channel curvature enhanced mixing and decreased particle size dependent on the velocity of the generated Dean flow.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2021)
Article
Pharmacology & Pharmacy
Rutika Godse, Maitreyi Rathod, Abhijit De, Ujwala Shinde
Summary: This study developed a novel sugar receptor-targeted drug delivery system for retinoblastoma cells using etoposide as a model drug. The system showed high specificity and cytotoxicity, demonstrating potential as a new therapeutic approach for retinoblastoma.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Haifeng Liang, Yiran Yan, Wei Sun, Xiaogang Ma, Zhiwen Su, Zhongxun Liu, Yan Chen, Bo Yu
Summary: This study evaluates the effects of melatonin on ROS and the innate immune response system in OA chondrocytes and the therapeutic effect in vivo, and proposes a new approach for treating osteoarthritis using a melatonin-loaded nano-delivery system. The results show that melatonin improves cartilage matrix metabolism and delays the progression of OA by inhibiting the TLR2/4-MyD88-NFκB signal pathway and scavenging ROS. The melatonin-loaded nano-delivery system can accumulate in cartilage, reduce the number of intra-articular injections, and improve the utilization rate of melatonin in vivo.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Chemical
Wei Li, Qiaoli Chen, Thejus Baby, Song Jin, Yun Liu, Guangze Yang, Chun-Xia Zhao
Summary: This study investigated the fundamental principles of polymer nanoparticle drug encapsulation through microfluidic technology. Using curcumin and PEG-PLGA as a model system, the research revealed the different precipitation and mixing times of the two components in the microfluidic device, leading to low drug loading. This provides a new perspective on understanding and controlling the formation of drug-loaded polymeric nanoparticles and offers guidelines for selecting the appropriate combinations of drugs, polymers, solvents, and devices.
CHEMICAL ENGINEERING SCIENCE
(2021)
Article
Nanoscience & Nanotechnology
Braham Mezghrani, Lamiaa M. A. Ali, Sebastien Richeter, Jean-Olivier Durand, Peter Hesemann, Nadir Bettache
Summary: The study introduces periodic mesoporous ionosilica nanoparticles (PMINPs) as versatile nano-objects for imaging, photodynamic therapy (PDT), and efficient delivery of small interfering RNA (siRNA) in breast cancer cells, demonstrating a triple ability to perform imaging, PDT, and PCI.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Chemistry, Multidisciplinary
Jun-Young Park, Jun Young Park, Yong-Gyu Jeong, Joo-Hwan Park, Yeon Ho Park, Sang-Hyun Kim, Dongwoo Khang
Summary: Due to stem cells' ability to specifically target tumor environments, stem-cell-membrane-functionalized nanocarriers can be used for targeted delivery of active anticancer drugs. This study focuses on self-targeting pancreatic cancer cells using stem cells. By utilizing the targeting abilities of stem cells, nano-polylactide-co-glycolide loaded with doxorubicin can be encapsulated within stem cell membranes, leading to targeted reduction of deep pancreatic tumor tissues. This platform technology can be applied to malignant tumors lacking surface target receptors, such as pancreatic tumor cells.
ADVANCED MATERIALS
(2023)
Review
Chemistry, Multidisciplinary
Chanda Bhandari, Mina Guirguis, N. Anna Savan, Navadeep Shrivastava, Sabrina Oliveira, Tayyaba Hasan, Girgis Obaid
Summary: Near infrared (NIR) photodynamic activation plays a crucial role in cutting-edge anti-cancer nanomedicines, with molecular targeted photonanomedicines (mt-PNMs) specifically targeting tumor tissue treatment with high precision. However, mt-PNMs are complex and face challenges, while providing opportunities to enhance ligand targeting effects. Both tumor specificity and selectivity are discussed with a focus on NIR photoactivable biomimetic nanotechnologies for safer and more effective photodynamic anti-tumor regimens.
Article
Biochemistry & Molecular Biology
Sebas D. Pronk, Erik Schooten, Jurgen Heinen, Esra Helfrich, Sabrina Oliveira, Paul M. P. van Bergen En Henegouwen
Summary: Antibody-drug conjugates (ADCs) are currently used for targeted drug delivery, but the large size and slow internalization of antibodies may limit their efficacy. By developing internalizing single domain antibodies (sdAbs) through phage display method, it is shown that sdAbs with high internalization rates have improved cytotoxicity when conjugated with cytotoxic drugs, indicating the importance of lysosomal trafficking for efficient drug release.
Correction
Chemistry, Multidisciplinary
Piet J. Driest, Jacobus A. W. Jong, Robert Smakman, Marc-Etienne Moret, Marianne C. Verhaar, Wim E. Hennink, Karin G. F. Gerritsen, Cornelus F. van Nostrum
Review
Biochemical Research Methods
Sanne M. van de Looij, Erik R. Hebels, Martina Viola, Mathew Hembury, Sabrina Oliveira, Tina Vermonden
Summary: Over the past two decades, atomic gold nanoclusters have shown promising applications in cancer diagnosis and treatment, with their native luminescent properties and the use of active targeting strategies contributing to their extensive potential in biomedical imaging and therapy.
BIOCONJUGATE CHEMISTRY
(2022)
Article
Pharmacology & Pharmacy
Charis Rousou, Josanne de Maar, Boning Qiu, Kim van der Wurff-Jacobs, Marika Ruponen, Arto Urtti, Sabrina Oliveira, Chrit Moonen, Gert Storm, Enrico Mastrobattista, Roel Deckers
Summary: The combination of ultrasound and microbubbles (USMB) can enhance drug permeability across tissue barriers, especially for larger and more hydrophilic molecules. USMB treatment leads to increased intracellular accumulation of molecules and does not significantly affect the permeability of small molecules. USMB can also improve the paracellular permeability and binding ability of clinically relevant nanobodies.
Review
Medicine, Research & Experimental
Mahsa Bagheri, Cornelus F. van Nostrum, Robbert Jan Kok, Gert Storm, Wim E. Hennink, Michal Heger
Summary: This review investigates whether intravenous nanoformulations of curcumin improve its pharmacokinetics and whether improved pharmacokinetics leads to greater therapeutic efficacy. The study found that nanoencapsulation generally improved curcumin's pharmacokinetics, but the correlation between pharmacokinetics and pharmacodynamics was not significant.
MOLECULAR PHARMACEUTICS
(2022)
Article
Pharmacology & Pharmacy
Lisa Marinelli, Michele Ciulla, Jeffrey A. S. Ritsema, Cornelus F. van Nostrum, Ivana Cacciatore, Marilisa Pia Dimmito, Ferdinando Palmerio, Giustino Orlando, Iole Robuffo, Rossella Grande, Valentina Puca, Antonio Di Stefano
Summary: The aim of this study was to encapsulate antimicrobial peptide FS10 in PEG-PLGA nanoparticles to overcome the limitations of hydrophilic drugs. The pH value and polymeric composition were found to be influential parameters for encapsulation efficiency. FS10-PEG-PLGA nanoparticles obtained under optimized parameters showed good size and surface potential, with sustained drug release. Antimicrobial assay demonstrated that the FS10-loaded nanoparticles had a lower minimum inhibitory concentration against S. aureus.
Article
Biochemistry & Molecular Biology
Barbara S. Mesquita, Marcel H. A. M. Fens, Alessia Di Maggio, Esmeralda D. C. Bosman, Wim E. Hennink, Michal Heger, Sabrina Oliveira
Summary: This study demonstrates that adjusting the density of nanobodies (Nbs) on nanoparticles (NPs) can increase the specificity of NPs towards target cells and reduce their interaction with phagocytic cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Medicinal
Mengshan Liu, Chun Yin Jerry Lau, Irene Trillo Cabello, Johan Garssen, Linette E. M. Willemsen, Wim E. Hennink, Cornelus F. van Nostrum
Summary: Our previous study showed that BLG-Pep loaded in PLGA nanoparticles could protect mice from cow's milk allergy. However, the mechanism of interaction with DCs and the fate of the nanoparticles were unclear. FRET was used to investigate these processes.
Article
Polymer Science
Myriam Neumann, Greta di Marco, Dmitrii Iudin, Martina Viola, Cornelus F. van Nostrum, Bas G. P. van Ravensteijn, Tina Vermonden
Summary: In the past decade, stimuli-responsive hydrogels have gained increasing attention in the field of tissue engineering and regenerative medicine. These smart hydrogels can replicate the physicochemical properties of the extracellular matrix and mimic dynamic processes that regulate cell behavior. By controlling the hydrogel properties or applying localized triggers, swelling-shrinking, shape-morphing, and patterns can be achieved to influence cell function in a spatiotemporally controlled manner. Additionally, stimuli-responsive hydrogels show great potential as bioinks for 4D bioprinting, enabling the fabrication of complex microstructures.
Article
Pharmacology & Pharmacy
Peter Bitsch, Eva S. Baum, Irati Beltran Hernandez, Sebastian Bitsch, Jakob Harwood, Sabrina Oliveira, Harald Kolmar
Summary: In this study, nanobody dextran polymer conjugates (dextraknobs) loaded with small molecules were developed for cancer diagnostics and therapy. It was found that using smaller sized dextrans improved the homogeneous penetration of the conjugates into tumor spheroids. Additionally, the dextraknobs showed potent cell killing effects in photodynamic therapy. The monovalent nanobody conjugates exhibited deeper and more homogeneous penetration compared to the bivalent conjugates. These findings encourage further development of dextraknobs.
Review
Medicine, Research & Experimental
Vida Mashayekhi, Orsola Mocellin, Marcel H. A. M. Fens, Gerard C. Krijger, Lodewijk A. A. Brosens, Sabrina Oliveira
Summary: This review discusses the challenges in the treatment and early diagnosis of pancreatic ductal adenocarcinoma (PDAC), emphasizing the need for more effective and better-tolerated treatment options. It also highlights the discovery of transmembrane proteins that are overexpressed in PDAC cells, which could potentially serve as targets for treatment and diagnosis.
Article
Nanoscience & Nanotechnology
Irati Beltran Hernandez, Guillaume C. M. Grinwis, Alessia Di Maggio, Paul M. P. van Bergen en Henegouwen, Wim E. Hennink, Erik Teske, Jan W. Hesselink, Sebastiaan A. van Nimwegen, Jan A. Mol, Sabrina Oliveira
Summary: NB-PDT utilizes nanobodies and photosensitizer to deliver therapeutic agents to cancer cells, causing cell death upon light irradiation. Successful treatment of feline OSCC and identification of EGFR as a relevant target have been demonstrated through experiments.
Article
Medicine, Research & Experimental
Katerina T. Xenaki, Bram Dorrestijn, Joey A. Muns, Kevin Adamzek, Sofia Doulkeridou, HendrikJan Houthoff, Sabrina Oliveira, Paul M. P. van Bergen en Henegouwen
Summary: Extending the half-life of nanobodies through non-covalent interaction with albumin can enhance their accumulation in tumors and improve therapeutic efficacy.
Review
Medicine, Research & Experimental
Irati Beltran Hernandez, Jannes Z. Kromhout, Erik Teske, Wim E. Hennink, Sebastiaan A. van Nimwegen, Sabrina Oliveira
Summary: Innovative approaches are necessary for cancer treatment, with targeted therapies offering tumor specificity and reduced side effects. However, the low translation success rate highlights the need for improvement. Comparative oncology can aid therapy refinement and increase translational success by introducing companion animals with spontaneous tumors.