Article
Microbiology
Ekaterina Krumova, Radoslav Abrashev, Vladislava Dishliyska, Galina Stoyancheva, Nedelina Kostadinova, Jeny Miteva-Staleva, Boryana Spasova, Maria Angelova
Summary: Cold-active catalase (CAT) produced by Penicillium griseofulvum P29 from Antarctic soil showed maximum activity under specific culture conditions. Optimizing the oxygen level in the bioreactor led to enhanced enzyme activity and productivity. The enzyme exhibited high activity at approximately 20 degrees C and pH 6.0, with thermostability ranging from 5 degrees C to 40 degrees C.
JOURNAL OF BASIC MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Ahmad Mohamed Moharram, Abdel-Naser Ahmed Zohri, Abd El-Latif Hesham, Hossam E. F. Abdel-Raheam, Mohamed Al-Ameen Maher, Osama Abdel-Hafeez Al-Bedak
Summary: Three new Cladosporium species capable of producing cold-active pectinases have been discovered and characterized. The cold-active pectinase produced by C. parasphaerospermum showed the highest activity in submerged fermentation. The purified pectinase displayed the highest activity and inhibitory effects under specific conditions. The addition of pure pectinase improved juice yield and clarity, as well as enhanced color. This study is important for pectinase production, waste decomposition, and juice clarification.
SCIENTIFIC REPORTS
(2022)
Article
Biology
Mary Arpana, Aneesa Fasim, H. K. Manjushree, Sunil S. More
Summary: The present study focuses on the characterization of a highly alkalophilic and thermostable polygalacturonase (PGase) from Pseudomonas sp. 13,159,349 and its potential use in olive-oil extraction. The PGase enzyme was produced using wheat bran solid substrate fermentation (SSF) media and purified by gel permeation chromatography. The enzyme exhibited optimum activity at pH 9 and 45 - 50 degrees C and retained stability at alkaline pH and high temperatures. The enzyme's kinetic parameters with pectin substrate were determined, and the effects of metal ions and surfactants on enzyme activity were investigated. Treatment with PGase significantly increased olive oil output and polyphenol content in olive paste.
Article
Biochemistry & Molecular Biology
Marta Wanarska, Ewelina Krajewska-Przybyszewska, Monika Wicka-Grochocka, Hubert Cieslinski, Anna Pawlak-Szukalska, Aneta M. Bialkowska, Marianna Turkiewicz, Tomasz Florczak, Ewa Gromek, Joanna Krysiak, Natalia Filipowicz
Summary: Yeasts are attractive host/vector systems for heterologous gene expression. A psychrotolerant yeast strain Debaryomyces macquariensis D50 has been developed as an expression system for the synthesis of thermolabile proteins and proteins that tend to form insoluble aggregates at low temperatures.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Guy Condemine, Bastien Le Derout
Summary: Dickeya are plant pathogenic bacteria that can cause diseases in a wide range of plants. The Out type 2 secretion system (T2SS) is essential for the virulence of Dickeya. Previous studies have shown that the T2SS of D. dadantii secretes various plant cell wall degrading enzymes, including pectinases and cellulases. However, the full range of exoproteins that it can secrete has not yet been fully identified. In this study, we identified four new Out-secreted proteins and demonstrated the importance of two of these proteins in the virulence of D. dadantii.
Article
Multidisciplinary Sciences
Young Sook Kim, Mirjalol Umurzokov, Kwang Min Cho, Jung Sup Choi, Kee Woong Park
Summary: This study focused on screening, characterizing and identifying insecticidal compounds from Streptomyces sp. KR0006. The isolated strain showed significant insecticidal activity against Plutella xylostella larvae and moths. Three active compounds were isolated and identified, and mutagenesis increased the insecticidal activity of the strain. The findings suggest that Streptomyces sp. KR0006 could be used for practical pest management.
Article
Energy & Fuels
Amr H. Hashem, Gadallah Abu-Elreesh, Hussein H. El-Sheikh, Waleed B. Suleiman
Summary: This study isolated seven oleaginous fungal strains from dung samples in the Egyptian ecosystem and investigated one strain, Mucor racemosus, for lipid accumulation. Statistical optimization of M. racemosus for lipid production was carried out, and the best temperature range for unsaturated fatty acids production was found to be between 10 and 20 degrees C. The high lipid yield of M. racemosus could be used for biodiesel production, while the unsaturated fatty acids produced may have potential pharmaceutical applications.
BIOMASS CONVERSION AND BIOREFINERY
(2023)
Article
Biochemistry & Molecular Biology
Zahoor Ahmad Baba, Basharat Hamid, Tahir Ahmad Sheikh, Saad H. Alotaibi, Hesham A. El Enshasy, Mohammad Javed Ansari, Ali Tan Kee Zuan, R. Z. Sayyed
Summary: This research isolated 13 strains of K solubilizing bacteria from the rhizosphere soils of northern India, which showed significant mineral K solubilizing activity at different temperatures and incubation periods. Among them, the isolate KSB (Grz) demonstrated the highest K solubilization index and solubilized the maximum amount of K after 48 hours of incubation at 20 degrees C.
Article
Biotechnology & Applied Microbiology
Junhua Zhang, Xuehua Yu, Bo Guan, Youzhen Hu, Xu Li, Jun Zeng, Yongqing Ni
Summary: In this study, a cold-adapted trehalase enzyme was isolated and characterized from a psychrotolerant bacterium. The enzyme showed high catalytic activity and specificity, suggesting its potential applications in various industries.
FERMENTATION-BASEL
(2022)
Article
Chemistry, Medicinal
Jiang Chen, Shanwen Zhang, Yingying Chen, Xinpeng Tian, Yucheng Gu, Jianhua Ju
Summary: SCSIO07399 strain of Verrucosispora sp. produces ansamycin-like polyketides kendomycin B-D with potent antibacterial activities and moderate tumor cytotoxicity. The biosynthetic gene cluster for kendomycin B was identified, and the essential role of the kmy cluster in biosynthesis was confirmed through gene disruption and heterologous expression. Insights into kendomycin B biosynthesis were gained and a foundation was set for further research into exploiting the carbacylic ansa scaffold.
Article
Microbiology
Sascha Jenkins, Andrew Taylor, Alison C. Jackson, Andrew D. Armitage, Helen J. Bates, Andrew Mead, Richard J. Harrison, John P. Clarkson
Summary: Fusarium oxysporum is a soilborne fungal plant pathogen causing Fusarium wilt disease of pea, with resistant cultivars commonly used for control but facing the risk of pathogen breakdown due to variations. Further research is needed to determine if differences in virulence across a broader set of pea cultivars are related to variations in SIX genes.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Iga Jodlowska, Aleksandra Twarda-Clapa, Kamil Szymczak, Aneta M. Bialkowska
Summary: The article discusses the screening of Antarctic fungi strains for MAO activity, leading to the discovery of a novel psychrozyme MAOP3 with wide substrate specificity and good activity potential.
Article
Microbiology
Amanda M. M. Starr, Masoud Zabet-Moghaddam, Michael San Francisco
Summary: This study identifies previously unreported secreted metabolites produced by B. dendrobatidis. The effects of these compounds on the pathogenicity traits of the fungus were evaluated using a non-amphibian model system.
Article
Environmental Sciences
Saba Miri, Jose Alberto Espejel Perez, Satinder Kaur Brar, Tarek Rouissi, Richard Martel
Summary: In this study, cold-active ToMO and C1,2D were produced using a newly isolated psychrophilic Pseudomonas S2TR-14 in a minimal salt medium supplemented with crustacean waste and different concentrations of used motor oil. Immobilized enzymes on micro/nano biochar-chitosan matrices showed high enzyme production and increased storage stability, leading to efficient degradation of BTEX in contaminated groundwater and soil. Integrated use of microbiochar and nanobiochar with chitosan for co-immobilization of ToMO and C1,2D can be a potential way to remove petroleum hydrocarbons with higher efficiency from contaminated sites.
ENVIRONMENTAL POLLUTION
(2021)
Article
Microbiology
Nikita Alkin, Yakov Dunaevsky, Elena Elpidina, Galina Beljakova, Valeria Tereshchenkova, Irina Filippova, Mikhail Belozersky
Summary: Proline-specific peptidases (PSP) are essential for processing fungal toxins, pheromones, and intracellular signaling, with potential applications in biotechnology. In silico analysis of PSP homologs in 42 species of higher fungi revealed characteristic homologs from various kingdoms and families of peptidases. Phylogenetic analysis suggested a duplication of ancestral DPP4 into transmembrane and secreted versions, predating the split of ascomycete and basidiomycete lineages. Comparative biochemical analysis showed the activity and stability of DPP4 under alkaline conditions and high salt concentrations in alkaliphilic and alkali-tolerant fungal strains.
Article
Biochemistry & Molecular Biology
Melissa Gomez, Maria Soledad Gutierrez, Ana Maria Gonzalez, Carla Garate-Castro, Dionisia Sepulveda, Salvador Barahona, Marcelo Baeza, Victor Cifuentes, Jennifer Alcaino
JOURNAL OF LIPID RESEARCH
(2020)
Article
Microbiology
Maximiliano Venegas, Salvador Barahona, Ana Maria Gonzalez, Dionisia Sepulveda, Gustavo E. Zuniga, Marcelo Baeza, Victor Cifuentes, Jennifer Alcaino
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Melissa Gomez, Sebastian Campusano, Maria Soledad Gutierrez, Dionisia Sepulveda, Salvador Barahona, Marcelo Baeza, Victor Cifuentes, Jennifer Alcaino
JOURNAL OF LIPID RESEARCH
(2020)
Article
Microbiology
Marcelo Baeza, Sergio Zuniga, Vicente Peragallo, Salvador Barahona, Jennifer Alcaino, Victor Cifuentes
Summary: Microorganisms inhabiting cold environments have evolved strategies to tolerate and thrive in extreme conditions, mainly through the synthesis of cold-active proteins/enzymes. Cold-adapted yeasts isolated from Antarctica exhibit differences in growth rate and genome characteristics, with correlations observed between the flexible amino acid content of predicted proteins and their optimal growth temperatures and rates.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ana-Maria Gonzalez, Maximiliano Venegas, Salvador Barahona, Melissa Gomez, Maria-Soledad Gutierrez, Dionisia Sepulveda, Marcelo Baeza, Victor Cifuentes, Jennifer Alcaino
Summary: This study describes the regulatory role of the Xanthophyllomyces dendrorhous DAP1 gene in yeast pigmentation and ergosterol biosynthesis. The deletion of DAP1 gene resulted in decreased astaxanthin fraction, increased beta-carotene fraction, and decreased ergosterol fraction. Coimmunoprecipitation experiments demonstrated the interaction between Dap1 and three P450 proteins. These findings suggest that Dap1 regulates the synthesis of astaxanthin and ergosterol in X. dendrorhous at the protein level.
JOURNAL OF LIPID RESEARCH
(2022)
Article
Microbiology
Marcelo Baeza, Sergio Zuniga, Vicente Peragallo, Fernando Gutierrez, Salvador Barahona, Jennifer Alcaino, Victor Cifuentes
Summary: Microorganisms have developed various strategies to adapt to cold environments, including the synthesis of protective molecules, regulation of proteins and membrane fluidity. The response to cold can vary among microorganisms and is influenced by the characteristics of the cold environment and seasonal temperature changes.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Pilar Martinez-Moya, Sebastian Campusano, Dionisia Sepulveda, Alberto Paradela, Jennifer Alcaino, Marcelo Baeza, Victor Cifuentes
Summary: Xanthophyllomyces dendrorhous, a yeast that produces astaxanthin and mycosporines, has been shown to have a survival strategy against stressful environments. The transcription factors SKN7, ROX1, and YAP6 have been found to play important roles in regulating the synthesis of these secondary metabolites. The proteome and transcriptome analysis revealed significant metabolic changes in the mutant strains, particularly in the glycolysis/gluconeogenesis, pentose phosphate pathway, and citrate cycle. Absence of ROX1 resulted in a significant decline in carotenoid production, while the absence of SKN7 and YAP6 led to an increase in mycosporine and sterol synthesis, respectively.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Dionisia Sepulveda, Sebastian Campusano, Martin Moline, Salvador Barahona, Marcelo Baeza, Jennifer Alcaino, Fernando Colabella, Blanca Urzua, Diego Libkind, Victor Cifuentes
Summary: Researchers discovered a 7873 bp cluster in the genome of Phaffia rhodozyma UCD 67-385 that contains genes encoding key enzymes in the mycosporine glutaminol (MG) biosynthesis pathway. Mutants with deletions or single-gene mutations of the cluster were unable to produce mycosporines. However, a mutant with the ATPG gene deleted accumulated the intermediate 4-deoxygadusol. Heterologous expression of the DDGS and OMT or DDGS, OMT, and ATPG genes in Saccharomyces cerevisiae resulted in production of 4-deoxygadusol or MG, respectively.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biology
Melissa Gomez, Marcelo Baeza, Victor Cifuentes, Jennifer Alcaino
Summary: Xanthophyllomyces dendrorhous is a basidiomycete yeast that produces the antioxidant carotenoid astaxanthin. The biosynthesis of carotenoids and sterols in X. dendrorhous share common elements, including metabolites from the mevalonate pathway and cytochrome P450 enzymes. The regulation of carotenoid biosynthesis in X. dendrorhous involves the SREBP pathway, known for its role in lipid metabolism control.
BIOLOGICAL RESEARCH
(2021)
Article
Biodiversity Conservation
Kiara Fernandez, Jennifer Alcaino, Dionisia Sepulveda, Rodrigo Medel
REVISTA CHILENA DE HISTORIA NATURAL
(2020)