Article
Biochemical Research Methods
Judith Fischer, Mascha Treblin, Tobias Sitz, Sascha Rohn
Summary: The use of algae and cyanobacteria in human nutrition is becoming increasingly important, with health effects believed to be influenced by minor components such as sulfolipids. Studies have found compounds like sulfoquinovosyldiacylglycerols (SQDGs) to play a role in the health effects of algae and cyanobacteria.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Food Science & Technology
Antonio Costa-Perez, Diego A. Moreno, Paula M. Periago, Cristina Garcia-Viguera, Raul Dominguez-Perles
Summary: The stalks of broccoli contain a significant amount of bioactive compounds, such as (poly)phenols and glucosinolates, which have antioxidant and anti-inflammatory properties. This study aimed to develop a new ingredient rich in these bioactive compounds from broccoli stalks and evaluate its bioaccessibility. The findings showed that low-temperature stabilization of the core samples provided the highest content of bioactives, including antioxidant phenolics and sulforaphane. These results suggest the potential for using broccoli stalks as a source of functional ingredients with health benefits.
Article
Agriculture, Multidisciplinary
Sinah Reiter, Andreas Dunkel, Corinna Dawid, Thomas Hofmann
Summary: Bile acids are increasingly investigated as markers for various diseases in humans and animals. A high-throughput workflow was used for bile acid analysis in animal studies, mapping complex bile acid profiles in tissues and body fluids. This method can also be used for screening food and studying the bioavailability of bile acids in the GI tract.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Food Science & Technology
Jing Wan, Peng He, Yuanshan Chen, Qiujin Zhu
Summary: A practical, efficient, and rapid method for quantifying 19 pyrethroids in tea and orange samples was developed. Out of 262 tea samples, 11 pyrethroids were detected positive with concentrations below maximum residue limits. No pyrethroid was found in 209 peeled orange samples.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Environmental Sciences
Lingyu Hu, Xiaofang Wang, Zhiwei Bao, Qihao Xu, Mingrong Qian, Yuanxiang Jin
Summary: The fungicide prothioconazole and its metabolite prothioconazole-desthio have harmful effects on the liver-gut axis of mice, altering bile acid metabolism, intestinal microbiota composition, and gut barrier function.
Article
Biochemistry & Molecular Biology
Maria Campillo, Sonia Medina, Federico Fanti, Juana I. Gallego-Gomez, Agustin Simonelli-Munoz, Valerie Bultel-Ponce, Thierry Durand, Jean Marie Galano, Francisco A. Tomas-Barberan, Angel Gil-Izquierdo, Raul Dominguez-Perles
Summary: The study demonstrates the potential of plant oxylipins in modulating inflammatory processes and protecting cells from inflammation, suggesting that specific plant oxylipins can prevent inflammatory events.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Pharmacology & Pharmacy
Hui Xu, Li-Bin Pan, Hang Yu, Pei Han, Jie Fu, Zheng-Wei Zhang, Jia-Chun Hu, Xin-Yu Yang, Adili Keranmu, Hao-Jian Zhang, Meng-Meng Bu, Jian-Dong Jiang, Yan Wang
Summary: Gut microbiota metabolites play a crucial role in inflammatory diseases, affecting various inflammatory conditions. Dysbiosis was observed in the gut microbiota of a rheumatoid arthritis model, with changes in the levels of phylum, genus, and alpha biodiversity of gut bacteria. Therapeutic drugs were found to impact the metabolites in the tryptophan and phenylalanine pathways of gut microbiota.
FRONTIERS IN PHARMACOLOGY
(2022)
Review
Immunology
Jing Liu, Dakai Yang, Xiaojing Wang, Paul Tetteh Asare, Qingwen Zhang, Lixin Na, Lei Shao
Summary: The liver and intestines are directly connected through the portal vein, allowing the gut microbiota and gut-derived products to influence liver health. Patients with chronic liver diseases, including non-alcoholic/alcoholic liver disease, chronic hepatitis virus infection, primary sclerosing cholangitis, and liver cirrhosis, have shown decreased gut flora diversity and increased sensitivity to alcohol. These patients also have increased intestinal mucosal permeability and decreased barrier function, leading to bacterial translocation and endotoxin uptake, which causes systemic inflammation. Specific changes in microbiota and microbiota-derived metabolites have been observed in chronic liver diseases, but the complex interaction between the gut microbiota and liver is not yet fully understood. This review discusses the bidirectional relationship between the gut and the liver and explores how alterations in the gut microbiota ecosystem affect the pathogenesis of chronic liver diseases. Additionally, gut-microbiota targeted interventions are proposed as a promising new method for managing chronic liver diseases.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Food Science & Technology
Yibin Lan, Min Liu, Xinke Zhang, Siyu Li, Ying Shi, Changqing Duan
Summary: The environmental conditions of wine regions determine the chemical composition and sensory characteristics of young Marselan wines. Using analytical methods such as gas chromatography-mass spectrometry and high-performance liquid chromatography, it was found that the concentrations of volatile compounds can differentiate young Marselan wines from different regions. The compounds and sensory attributes of Marselan wines are related to the specific environmental conditions of the wine-producing regions.
Article
Agriculture, Multidisciplinary
Zunji Shi, Gui Chen, Zheng Cao, Fang Wu, Hehua Lei, Chuan Chen, Yuchen Song, Caixiang Liu, Jinquan Li, Jinlin Zhou, Yujing Lu, Limin Zhang
Summary: This study evaluated the functional effects of bile acids (BAs) and gut microbiota on sucralose consumption-induced nonalcoholic fatty liver disease (NAFLD) in mice. Results showed that sucralose consumption increased the abundance of intestinal genera Bacteroides and Clostridium, leading to elevated hepatic deoxycholic acid (DCA) levels which inhibited hepatic gene expression and contributed to NAFLD. Dietary supplements with fructo-oligosaccharide or metformin restored the gut microbiota and DCA levels, thereby ameliorating NAFLD in sucralose-consuming mice. These findings highlighted the important role of gut microbiota and DCA in the development of NAFLD induced by sucralose.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Yifeng Fu, Siying Li, Yunhua Xiao, Gang Liu, Jun Fang
Summary: This study explores the importance of microbiota metabolites in type 2 diabetes (T2D), as well as the role of medication and dietary components in reducing the risk of developing diabetes. A deeper understanding of the link between gut microbial metabolites and T2D will enhance our knowledge of the disease and may offer new treatment approaches.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Simona Ceglia, Alyssa Berthelette, Kelsey Howley, Yun Li, Benedikt Mortzfeld, Shakti K. Bhattarai, Nicole K. H. Yiew, Ying Xu, Robert Brink, Jason G. Cyster, Lora V. Hooper, Gwendalyn J. Randolph, Vanni Bucci, Andrea Reboldi
Summary: Research shows that high-cholesterol diets can influence IgA secretion. Cholesterol-derived metabolites affect the positioning of IgA(+) plasma cells and suppress antibody responses to intestinal pathogens by acting on GPR183 receptors.
Article
Food Science & Technology
Gangzheng Wang, Xianglian Chen, Chengyuan Sun, Qiuju Huang, Chenghua Zhang, Min Lin, Jianping Xu, Xueshuang Huang, Wangqiu Deng
Summary: Poria cocos has beneficial health effects by modulating gut microbiota and metabolites, which can alleviate obesity and related complications.
JOURNAL OF FUNCTIONAL FOODS
(2023)
Article
Microbiology
Jay Hind Nishad, Arti Singh, Rajnish Bharti, Priyanka Prajapati, Vijay Kumar Sharma, Vijai Kumar Gupta, Ravindra Nath Kharwar
Summary: The study demonstrated that the extract of D. longicolla treated with BRD4770 showed increased antioxidant activity and inhibition zone against S. aureus and MRSA. Analysis revealed induced antibacterial and antioxidant-like compounds in the BRD4770-treated extract, supported by mass spectrometry and high-performance liquid chromatography.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Nutrition & Dietetics
Vicente Agullo, Cristina Garcia-Viguera, Raul Dominguez-Perles
Summary: This study aimed to develop new beverages rich in anthocyanins and flavanones, and evaluate the bioaccessibility and bioavailability of these compounds with alternative sweeteners. Results showed that stevia and sucralose could be valuable alternatives to sucrose based on their different bioavailability.
Editorial Material
Biotechnology & Applied Microbiology
Thomas Clavel, Hans-Peter Horz, Nicola Segata, Maria Vehreschild
Summary: Gut microbiome research has advanced significantly in the past 15 years, but there are still many unanswered questions about enteric microbial ecosystems. In the next 15 years, besides continuing research, organizational measures will also need to be taken to optimize the outcomes of discoveries.
MICROBIAL BIOTECHNOLOGY
(2022)
Article
Microbiology
Afrizal Afrizal, Thomas C. A. Hitch, Alina Viehof, Nicole Treichel, Thomas Riedel, Birte Abt, Eva M. Buhl, Dietrich Kohlheyer, Joerg Overmann, Thomas Clavel
Summary: The study demonstrates that the single-cell dispensing (SCD) approach significantly reduces the time required for obtaining pure cultures compared to the classical agar plate (CAP) approach. Both methods show comparable isolate diversity and relative abundance coverage. In addition, SCD-based cultivation captures more species that were not detected by sequencing, offering potential for deeper cultivation projects.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Medicine, Research & Experimental
Eva Michaud, Louis Waeckel, Remi Gayet, Roman Goguyer-Deschaumes, Blandine Chanut, Fabienne Jospin, Katell Bathany, Magali Monnoye, Coraline Genet, Amelie Prier, Caroline Tokarski, Philippe Gerard, Xavier Roblin, Nicolas Rochereau, Stephane Paul
Summary: The interaction between IgA and the gut microbiota may play a role in dysbiosis induction in inflammatory bowel diseases. Reverse-transcytosis is disrupted in ulcerative colitis, while extended in Crohn's disease. The interaction between IgA1 and the microbiota may have an impact on the composition of the gut microbiota.
EMBO MOLECULAR MEDICINE
(2022)
Article
Immunology
Jeanne Couturier, Patricia Lepage, Sarah Jolivet, Johanne Delannoy, Victoria Mesa, Pierre-Yves Ancel, Jean-Christophe Roze, Marie-Jose Butel, Frederic Barbut, Julio Aires
Summary: In preterm neonates, colonization by C. difficile is associated with alterations in the gut microbiota, with certain bacterial taxa positively or negatively correlated with the colonization. The colonization is related to the absence of postnatal antibiotic treatment and higher gestational age. There is no significant association between C. difficile colonization and necrotizing enterocolitis in preterm neonates.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Hematology
Nicolas Vallet, Sophie Le Grand, Louise Bondeelle, Benedicte Hoareau, Aurelien Corneau, Delphine Bouteiller, Simon Tournier, Lucille Derivry, Armelle Bohineust, Marie Tourret, Delphine Gibert, Ethan Mayeur, Raphael Itzykson, Kim Pacchiardi, Brian Ingram, Stephane Cassonnet, Patricia Lepage, Regis Peffault de Latour, Gerard Socie, Anne Bergeron, David Michonneau
Summary: Administration of azithromycin after stem cell transplantation is associated with relapse by altering immune cells and energy metabolism pathways, raising caution about its long-term use.
Article
Gastroenterology & Hepatology
Virginie Millet, Thomas Gensollen, Michael Maltese, Melanie Serrero, Nathalie Lesavre, Christophe Bourges, Christophe Pitaval, Sophie Cadra, Lionel Chasson, Thien Phong Vu Man, Marion Masse, Juan Jose Martinez-Garcia, Fabrice Tranchida, Laetitia Shintu, Konrad Mostert, Erick Strauss, Patricia Lepage, Mathias Chamaillard, Achille Broggi, Laurent Peyrin-Biroulet, Jean-Charles Grimaud, Philippe Naquet, Franck Galland
Summary: This study shows that the overexpression of Vnn1 is associated with the severity of IBD. VIVA mice are resistant to experimental colitis, and the pantetheinase activity of Vnn1 has a protective effect on colonocytes, enhancing CoA regeneration and metabolic adaptation. It also promotes the production of microbiota-dependent short chain fatty acids, especially butyrate, which regulates mucosal energetics and is reduced in patients with IBD. The induction of Vnn1/VNN1 during colitis is a compensatory mechanism to strengthen the mucosal barrier, and enhancing vitamin B-5-driven metabolism may improve mucosal healing and increase the efficacy of anti-inflammatory therapy.
Article
Food Science & Technology
Charline Buisson, Valentin Leuzy, Emmanuelle Loizon, Emmanuelle Meugnier, Magali Monnoye, Catherine Philippe, Philippe Gerard, Marie-Caroline Michalski, Fabienne Laugerette
Summary: This study investigates the effects of rapeseed oil enriched with soy lecithin (SPL) on white adipose tissue (WAT) inflammation, ileum permeability, and microbiota composition in mice. The results show that rapeseed oil with SPL leads to increased gene expression of leptin and inflammation in WAT, and decreased expression of TLR4, IAP, and tight junction proteins in the ileum. However, it also increases the expression of antimicrobial activity proteins and promotes the growth of certain bacterial species.
MOLECULAR NUTRITION & FOOD RESEARCH
(2023)
Review
Gastroenterology & Hepatology
Oliver Pabst, Mathias W. Hornef, Frank G. Schaap, Vuk Cerovic, Thomas Clavel, Tony Bruns
Summary: In this review, the gut-liver axis is discussed, focusing on the barriers formed by the enterohepatic tissues to restrict gut-derived microorganisms, microbial stimuli and dietary constituents. The establishment and regulation of structural and functional barriers, dynamics within the axis (immune responses and microbiome), and clinical implications are also highlighted.
NATURE REVIEWS GASTROENTEROLOGY & HEPATOLOGY
(2023)
Article
Microbiology
Kawtar Keddar, Hasnia Ziar, Noussaiba Belmadani, Magali Monnoye, Philippe Gerard, Ali Riazi
Summary: This study investigated the potential use of probiotic bacteria from human milk to alleviate oral cow's milk sensitization. The probiotic potential of the SL42 strain, isolated from a young healthy mother's milk, was determined. Rats were gavaged with cow's milk casein and treated with Limosilactobacillus reuteri DSM 17938, SL42, or a control solution. The results showed that SL42 reduced allergic responses, decreased histamine levels, CAS-specific IgE levels, eosinophil numbers, S100A8/9, and cytokine concentrations. Additionally, probiotic treatment increased Lactic acid bacteria and Clostridia species. These findings suggest the potential of probiotics from human milk to alleviate cow's milk casein allergy.
Article
Nutrition & Dietetics
Vivien Letenneur, Magali Monnoye, Catherine Philippe, Sophie Holowacz, Sylvie Rabot, Patricia Lepage, Elsa Jacouton, Laurent Naudon
Summary: This study found that supplementation with a probiotic mix can alleviate anxiety responses in mice sensitive to stress and have certain effects on gut microbiota. The probiotic mix can attenuate the weight loss and coat abnormalities caused by chronic stress and reduce stress-related anxiety-like behaviors. Additionally, serum corticosterone levels are significantly higher in the probiotic group, and the expression of dopamine and serotonin transporters in the hippocampus is lower. There are few differences in bacterial genera proportions and cecal acetate levels between the groups.
Article
Microbiology
Julio Aires, Zehra Esra Ilhan, Lancelot Nicolas, Laurent Ferraris, Johanne Delannoy, Maxime Bredel, Anne Chauvire-Drouard, Frederic Barbut, Jean-Christophe Roze, Patricia Lepage, Marie-Jose Butel
Summary: This study reports data from a French case-control prospective multicenter study, which found differences in gut microbiota composition between NEC cases and controls. The relative abundance of Lactobacillus genus was significantly lower in the NEC group, while that of the Clostridium cluster III was significantly higher. The study also found a statistical association between gut microbiota and cesarean delivery and antifungal therapy.
Article
Microbiology
Nacima Kouadri Boudjelthia, Meryem Belabbas, Nahla Bekenniche, Magali Monnoye, Philippe Gerard, Ali Riazi
Summary: This study aimed to screen new LAB from Algerian cow's milk and evaluate their probiotic properties. The LAB isolates were identified using molecular identification and MALDI-TOF mass spectrometry methods. In vitro tests showed high survival rate to gastrointestinal conditions and antimicrobial and antioxidant activities in the isolates. The results suggest that some strains from raw milk of local Algerian cows have promising probiotic properties for food preservation and storage by inhibiting microbial spoilage and oxidation.
Article
Microbiology
Ahlem Mahjoub Khachroub, Magali Monnoye, Nour Elhouda Bouhlel, Sana Azaiez, Maha Ben Fredj, Wejdene Mansour, Philippe Gerard
Summary: In this study, the gut microbiota of healthy Tunisian adults was characterized for the first time using 16S rRNA gene sequencing, and further compared based on gender and BMI. The results showed that the gut microbiota of Tunisian people is mainly composed of the phyla Firmicutes and Bacteroidota, in line with studies from western countries. However, some differences were found in terms of gender and overweight status. These findings provide an important baseline for identifying dysbiosis associated with major diseases in the Tunisian population.
FEMS MICROBIOLOGY LETTERS
(2023)
Article
Food Science & Technology
H. Ziar, I Yahla, M. Sadoud, K. Keddar, A. Dilmi-Bouras, A. Riazi, P. Gerard
Summary: This study demonstrated the use of raw locust bean gum (LBG) as a prebiotic, combined with different probiotic strains in fermented milk to achieve targeted release of bacteria under colon-like conditions. The experiment showed that LBG-enriched fermented milks supported better biomass and acidifying activities, while microencapsulated bacteria exhibited higher survival rates under simulated gastrointestinal conditions.
INTERNATIONAL FOOD RESEARCH JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Xufei Zhang, Philippe Gerard
Summary: Cardiovascular diseases (CVD) are the leading cause of morbidity and mortality worldwide. The gut microbiota plays a vital role in CVD development and can be influenced by dietary nutrients. The crosstalk between diet and microbiota regulates CVD progression through various mechanisms, including endotoxemia, inflammation, gut barrier dysfunction, and lipid metabolism dysfunction. Microbial metabolites such as trimethylamine-N-oxide (TMAO), secondary bile acids (BAs), short chain fatty acids (SCFAs), and aromatic amino acids (AAAs) contribute to CVD pathogenesis. Dietary interventions targeting the gut microbiota may serve as novel strategies for CVD management.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)