Article
Immunology
Ling-Cui Meng, Jia-Yi Zheng, Yu-Hui Qiu, Li Zheng, Jue-Yan Zheng, Yu-Qing Liu, Xiao-Lu Miao, Xin-Yi Lu
Summary: In this study, the protective effects of Salvianolic acid B (SalB) on non-alcoholic fatty liver disease (NAFLD) were investigated. The results showed that SalB significantly reduced body and liver weights, improved plasma markers of liver damage, and reduced hepatic lipid accumulation in a mouse model of NAFLD. SalB also inhibited inflammation and the activation of NLRP3 inflammasome. These findings suggest that SalB has potential as a hepatoprotective agent against NAFLD.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Dieuwertje Augustijn, Huub J. M. de Groot, A. Alia
Summary: Metabolomics is a method used to simplify the complexity of plants and understand the pathways of plant phenotypes. HR-MAS NMR can eliminate the extraction step of metabolites from samples, saving time and costs. HR-MAS NMR has important applications in plant metabolomics.
Article
Chemistry, Applied
Valeria Vanoli, Sara Delleani, Mose Casalegno, Fabio Pizzetti, Pooyan Makvandi, Havard Haugen, Andrea Mele, Filippo Rossi, Franca Castiglione
Summary: Hydrogels based on hyaluronic acid and agarose-carbomer have attracted attention as drug delivery systems. The structure of the polymer network greatly affects the release capability of these materials. Using HR-MAS NMR spectroscopy, this study investigated the motion of drugs within the hydrogels and found evidence of superdiffusive motion, which may be responsible for the faster release of sodium salicylate.
CARBOHYDRATE POLYMERS
(2023)
Article
Biochemical Research Methods
Marion Maravat, Marylene Bertrand, Celine Landon, Franck Fayon, Severine Morisset-Lopez, Vincent Sarou-Kanian, Martine Decoville
Summary: The study successfully induced gliomas in Drosophila and obtained metabolic profiles using nuclear magnetic resonance techniques. Statistical analyses revealed different metabolic signatures, impacted pathways, and glioma biomarkers.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Gastroenterology & Hepatology
Stephen A. Harrison, Guy Neff, Cynthia D. Guy, Mustafa R. Bashir, Angelo H. Paredes, Juan P. Frias, Ziad Younes, James F. Trotter, Nadege T. Gunn, Sam E. Moussa, Anita Kohli, Kristin Nelson, Mildred Gottwald, William C. G. Chang, Andrew Z. Yan, Alex M. DePaoli, Lei Ling, Hsiao D. Lieu
Summary: In a phase 2 trial of patients with NASH, aldafermin reduced liver fat content significantly and showed potential improvement in fibrosis.
Article
Biochemistry & Molecular Biology
Carolina da Silva Canielles Caprara, Tatiane Ksyvickas Mathias, Maria de Fatima C. Santos, Marcelo G. M. D'Oca, Caroline Da R. M. D'Oca, Fabio Roselet, Paulo Cesar Abreu, Daniela Fernandes Ramos
Summary: This study aimed to characterize the main metabolites of three species of microalgae using H-1 HR-MAS NMR. Although the three species were found in the same ecosystem and had a high production of lipid compounds, significant differences were identified in the production of specialized metabolites. These unique properties make these compounds potential leaders in the development of new bioactive compounds for One Health and alternatives for aquaculture.
Article
Biochemistry & Molecular Biology
Valtteri Virtanen, Susanna Raikkonen, Elina Puljula, Maarit Karonen
Summary: The study revealed that ellagitannins interacting with lipids share clear structural similarities, with all interacting ellagitannins possessing glucopyranose cores. The most important structural feature affecting the interaction appeared to be the structural flexibility of the ellagitannin, as even dimeric and trimeric ellagitannins could penetrate lipid bilayers if their structures were flexible.
Article
Nutrition & Dietetics
Usevalad Ustsinau, Viktoria Ehret, Clemens Fuernsinn, Thomas Scherer, Thomas H. Helbich, Marcus Hacker, Martin Krssak, Cecile Philippe
Summary: The worldwide prevalence of NAFLD has raised concerns about its associated risk factors, such as obesity and type 2 Diabetes Mellitus. In this study, the use of [F-18]FTHA PET tracer revealed differences in hepatic FFA uptake and incorporation into VLDL between healthy and NAFLD rats. This novel approach could contribute to a deeper understanding of the metabolic disturbances involved in the progression of NAFLD.
CLINICAL NUTRITION
(2023)
Article
Immunology
Fang Xie, Hang-Fei Xu, Jing Zhang, Xiao-Ni Liu, Bu-Xin Kou, Meng-Yin Cai, Jing Wu, Jin-Ling Dong, Qing-Hua Meng, Yi Wang, Dexi Chen, Yang Zhang
Summary: ASPP2 deficiency promotes NAFLD progression, alters fatty acid metabolism, and causes gut microbiota dysbiosis. EPA, a long-chain fatty acid, is significantly negatively correlated with the abundance of Bifidobacterium, a specific feature of NAFLD in ASPP2-deficient mice. The results provide evidence for the mechanism of ASPP2 in dysregulation of fatty acid metabolism and gut microbiota dysbiosis.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Karolina Krajewska, Anna M. Golkowska, Maciej Nowak, Marta Kozakiewicz-Latala, Wojciech Pudlo, Andrzej Zak, Bozena Karolewicz, Yaroslav Z. Khimyak, Karol P. Nartowski
Summary: This study proposes the use of HR-MAS NMR spectroscopy for a molecular level characterisation of the external surface of carbohydrate-functionalised nanoparticles. The results demonstrate that HR-MAS NMR can provide detailed structural information on the organic functionalities attached at the external surface of MSN within short experimental times.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Eric Simon, Maciej Motyka, Grietje H. Prins, Mei Li, Werner Rust, Stefan Kauschke, Coralie Viollet, Peter Olinga, Anouk Oldenburger
Summary: There is a need for predictive ex vivo models for NAFLD. This study profiles a new human and mouse PCLSs based assay for steatosis in NAFLD using RNASeq. Steatosis is induced by incremental supplementation of sugars, insulin, and fatty acids, and the gene expression regulation is analyzed across different nutrient conditions and time for both human and mouse liver organ derived PCLSs.
Article
Chemistry, Medicinal
Ha-Neul Choi, Jin-Yeong Shin, Jung-In Kim
Summary: The study found that myricetin has beneficial effects against NAFLD by regulating the expression of transcription factors of hepatic lipid metabolism, the antioxidant system, and pro-inflammatory cytokines.
JOURNAL OF MEDICINAL FOOD
(2021)
Article
Endocrinology & Metabolism
Fangli Zhou, Yan Wang, Yujue Li, Mengjia Tang, Shan Wan, Haoming Tian, Xiang Chen
Summary: The study found that circulating sclerostin levels were significantly lower in NAFLD patients compared to controls, and were negatively correlated with multiple metabolic parameters in NAFLD patients. Mice fed on a high-fat diet had reduced bone mass and lower sclerostin expression levels. Therefore, liver-lipid-bone interactions may play a key role in abnormal bone metabolism in NAFLD.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Cell Biology
Feng Cai, Divya Bezwada, Ling Cai, Rohit Mahar, Zheng Wu, Mario C. Chang, Panayotis Pachnis, Chendong Yang, Sherwin Kelekar, Wen Gu, Bailey Brooks, Bookyung Ko, Hieu S. Vu, Thomas P. Mathews, Lauren G. Zacharias, Misty Martin-Sandoval, Duyen Do, K. Celeste Oaxaca, Eunsook S. Jin, Vitaly Margulis, Craig R. Malloy, Matthew E. Merritt, Ralph J. Deberardinis
Summary: Stable isotopes are important tools for evaluating metabolism and can be detected using both mass spectrometry and nuclear magnetic resonance in vivo. We have developed a mass spectrometry method that can report all 16 aspartate and 32 glutamate isotopomers, offering higher sensitivity than nuclear magnetic resonance. This method allows for discrimination between pathways that result in the same number of 13C labels in aspartate and glutamate. Through experiments, we have demonstrated metabolic heterogeneity within human tumors, examined the impact of fumarate hydratase deficiency in renal cancer, and investigated the contributions of tricarboxylic acid cycle turnover and CO2 recycling to in vivo isotope labeling.
Article
Oncology
Zhouwei Wu, Tao Han, Haohan Su, Jiangwei Xuan, Xinwei Wang
Summary: This study aimed to comprehensively assess the fatty acid and lactate metabolism pattern in osteosarcoma patients and construct a risk score model based on this metabolism pattern to predict prognosis. The high-risk group showed lower survival time, which was associated with elevated fatty acids and lactate risk scores. The study provides essential insights into subsequent mechanisms and potential treatment strategies based on immune correlation analysis and drug sensitivity.
FRONTIERS IN ONCOLOGY
(2022)
Article
Polymer Science
Ahmad Telfah, Qais M. Al-Bataineh, Elen Tolstik, Ahmad A. Ahmad, Ahmad M. Alsaad, Riad Ababneh, Carlos J. Tavares, Roland Hergenroeder
Summary: This study synthesized PEO:I-2 complex composite films with different I-2 concentrations and investigated their optical and optoelectronic properties. It was found that the addition of I-2 increased the electrical conductivity of the composite and decreased the transmittance of PEO. Furthermore, the study revealed the reaction mechanism between PEO and I-2 through NMR spectroscopy analysis.
Article
Oncology
Magdalena Keller, Katharina Rohlf, Annika Glotzbach, Gregor Leonhardt, Simon Lueke, Katharina Derksen, Oezlem Demirci, Defne Goecener, Mohammad AlWahsh, Joerg Lambert, Cecilia Lindskog, Marcus Schmidt, Walburgis Brenner, Matthias Baumann, Eldar Zent, Mia-Lisa Zischinsky, Birte Hellwig, Katrin Madjar, Joerg Rahnenfuehrer, Nina Overbeck, Joerg Reinders, Cristina Cadenas, Jan. G. G. Hengstler, Karolina Edlund, Rosemarie Marchan
Summary: This study found that EDI3 is highly expressed in ER-HER2+ breast tumors, and its expression and activity are also highest in ER-HER2+ breast cancer cell lines. Silencing HER2 and inhibiting HER2 signaling can decrease EDI3 expression. Silencing EDI3 can selectively decrease cell viability in ER-HER2+ cells. Therefore, EDI3 may be a potential therapeutic target in patients with HER2-targeted therapy-resistant ER-HER2+ breast cancer.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2023)
Review
Health Care Sciences & Services
Sarah Friedrich, Andreas Groll, Katja Ickstadt, Thomas Kneib, Markus Pauly, Joerg Rahnenfuhrer, Tim Friede
Summary: This article reviews regularization approaches in data science for overcoming overfitting and improving prediction, and discusses their limited application in medical research. The authors suggest increased use of regularization approaches in medicine, despite the added complexity they bring to analyses. Proper investments in computing facilities and educational resources can help overcome these challenges.
STATISTICAL METHODS IN MEDICAL RESEARCH
(2023)
Article
Psychiatry
Johannes C. S. Zang, Caroline May, Birte Hellwig, Dirk Moser, Jan G. Hengstler, Steve Cole, Markus Heinrichs, Joerg Rahnenfuehrer, Katrin Marcus, Robert Kumsta
Summary: The experience of childhood adversity has been linked to poor health outcomes in adulthood. Previous research focused on DNA methylation and transcriptomic profiles, while neglecting the level of proteins. Using mass spectrometry, this study examined the proteome of CD14(+) monocytes in adults with childhood adversity before and after psychosocial stress exposure. The results indicated upregulation of specific proteins related to immune processes, protein metabolism, and mitochondrial biology, especially in females.
TRANSLATIONAL PSYCHIATRY
(2023)
Article
Biochemistry & Molecular Biology
Ilinca Suciu, Johannes Delp, Simon Gutbier, Anna-Katharina Ueckert, Anna-Sophie Spreng, Philipp Eberhard, Christiaan Karreman, Falk Schreiber, Katrin Madjar, Joerg Rahnenfuehrer, Ivana Celardo, Ivano Amelio, Marcel Leist
Summary: Proteasome inhibition is related to parkinsonian pathology and dopaminergic neuron degeneration. In this study, the metabolome and transcriptome regulations of human LUHMES neurons exposed to MG-132 were investigated, revealing compromised energy metabolism and activation of stress response pathways. These findings provide insights into the cellular responses to proteasome dysfunction and potential therapeutic interventions for Parkinson's disease.
Article
Medicine, General & Internal
Joerg Rahnenfuehrer, Riccardo De Bin, Axel Benner, Federico Ambrogi, Lara Lusa, Anne-Laure Boulesteix, Eugenia Migliavacca, Harald Binder, Stefan Michiels, Willi Sauerbrei, Lisa McShane
Summary: This review provides an overview of key aspects and methods for analyzing high-dimensional data in biomedical research. It addresses the challenges and opportunities of analyzing high-dimensional data, and offers solutions and references for researchers.
Article
Gastroenterology & Hepatology
Huan Su, Madhuri Haque, Svea Becker, Karolina Edlund, Julia Duda, Qingbi Wang, Johanna Reissing, Hanns-Ulrich Marschall, Lena S. Candels, Mohamed Mohamed, Wilhelm Sjoland, Lijun Liao, Stephan A. Drexler, Till Strowig, Joerg Rahnenfuehrer, Jan G. Hengstler, Maximilian Hatting, Christian Trautwein
Summary: This study used a mouse model to investigate the impact of the PNPLA3 I148M polymorphism on the progression of NAFLD. It was found that mice with the PNPLA3 I148M genotype showed exacerbated NAFLD, which was associated with changes in microbiota composition and liver gene expression, leading to a stronger inflammatory response and enhanced liver fibrosis progression.
LIVER INTERNATIONAL
(2023)
Article
Oncology
Katrin Almstedt, Lisa Krauthauser, Franziska Kappenberg, Daniel-Christoph Wagner, Anne-Sophie Heimes, Marco J. J. Battista, Katharina Anic, Slavomir Krajnak, Antje Lebrecht, Roxana Schwab, Walburgis Brenner, Wolfgang Weikel, Joerg Rahnenfuehrer, Jan G. G. Hengstler, Wilfried Roth, Annette Hasenburg, Kathrin Stewen, Marcus Schmidt
Summary: This study investigated the differences in HER2 expression levels between primary tumors and distant metastases in HER2-negative breast cancer patients. The results showed relevant discordance of HER2-low status between primary tumors and their corresponding distant metastases. Significant HER2 discordance rates were observed between different metastatic sites and molecular subtypes. These findings highlight the importance of evaluating potentially therapy-relevant discordance rates between a primary tumor and corresponding distant metastases.
Review
Toxicology
Franziska Kappenberg, Julia C. C. Duda, Leonie Schuermeyer, Onur Guel, Tim Brecklinghaus, Jan G. G. Hengstler, Kirsten Schorning, Joerg Rahnenfuehrer
Summary: The analysis of dose-response, concentration-response, and time-response relationships is a crucial aspect of toxicological research. Researchers must decide whether to solely consider measured concentrations or use a parametric model for extrapolation. This study examines the current state of statistical analysis in the toxicological literature through a comprehensive review of dose-response analyses published in three major journals in 2021. The review covers biological considerations, statistical design elements, and analysis aspects, providing concrete guidance for statistical planning, execution, and analysis of dose-response studies.
ARCHIVES OF TOXICOLOGY
(2023)
Article
Biochemical Research Methods
Leonie Schuermeyer, Kirsten Schorning, Joerg Rahnenfuehrer
Summary: An important problem in toxicology is the simultaneous inference of concentration-response relationships in gene expression data. The choice of experimental design and the set of concentrations used for observations greatly affect the quality of inference. In this study, we propose efficient designs for simultaneous inference and use D-optimality criterion and K-means procedure for clustering support points.
BMC BIOINFORMATICS
(2023)
Article
Biochemistry & Molecular Biology
Florian Seidel, Franziska Kappenberg, Susann Fayyaz, Andreas Scholtz-Illigens, Anna Cherianidou, Katharina Derksen, Patrick Nell, Rosemarie Marchan, Karolina Edlund, Marcel Leist, Agapios Sachinidis, Jorg Rahnenfuehrer, Reinhard Kreiling, Jan G. Hengstler
Summary: The study conducted a comparison of the four currently approved parabens and found that cytotoxicity and gene expression changes only occurred at high concentrations. It suggests that the results of in vivo DART with ethylparaben are similar to the methyl- or propyl derivates.
CHEMICO-BIOLOGICAL INTERACTIONS
(2023)
Article
Cell Biology
Anna Cherianidou, Franziska Kappenberg, Florian Seidel, Aviseka Acharya, Panagiota Papazoglou, Sureshkumar Perumal Srinivasan, Juergen Hescheler, Luying Peng, Marcel Leist, Jan G. Hengstler, Joerg Rahnenfuehrer, Agapios Sachinidis
Summary: Animal studies for embryotoxicity evaluation are limited in their relevance to humans due to species differences. This study used human induced pluripotent stem cells (hiPSCs) to recapitulate cardiomyogenesis and identify key genes and processes affected by potential teratogens. We found that certain retinoids completely blocked cardiomyogenesis in hiPSCs and established a Developmental Cardiotoxicity Index that accurately differentiates teratogens from non-teratogens.
CELL DEATH DISCOVERY
(2023)
Article
Toxicology
Marieke Stolte, Wiebke Albrecht, Tim Brecklinghaus, Lisa Gruendler, Peng Chen, Jan G. Hengstler, Franziska Kappenberg, Jorg Rahnenfuehrer
Summary: This article explores the use of dimension reduction methods and advanced statistical learning algorithms for classifying compounds based on hepatotoxic risk. The research shows that combining data from cytotoxicity assays and gene expression variables leads to better prediction accuracy than using these variables individually. Different strategies for summarizing gene expression data also contribute to improved prediction quality.
COMPUTATIONAL TOXICOLOGY
(2023)