Article
Agronomy
Dandan Cui, Shanshan Zhai, Yao Yang, Yuhua Wu, Jun Li, Xiaohong Yan, Ping Shen, Hongfei Gao, Gang Wu
Summary: An unmarked electrochemical impedance genosensor using gold carbon dots has been developed for the detection of genetically modified crops. This sensor is portable and easy to use, making it suitable for resource-limited laboratories.
Article
Biochemistry & Molecular Biology
Kevin Maafu Juma, Teisuke Takita, Masaya Yamagata, Mika Ishitani, Kaichi Hayashi, Kenji Kojima, Koichiro Suzuki, Yuri Ando, Wakao Fukuda, Shinsuke Fujiwara, Yukiko Nakura, Itaru Yanagihara, Kiyoshi Yasukawa
Summary: The study found that using N-terminal tagged uvsY (uvsY-Nhis) in recombinase polymerase amplification (RPA) can improve the reaction efficiency and is suitable for detecting SARS-CoV-2 DNA.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Medicine, General & Internal
Tingting Jiang, Yacui Wang, Weiwei Jiao, Yiqin Song, Qing Zhao, Tianyi Wang, Jing Bi, Adong Shen
Summary: In this study, a real-time recombinase polymerase amplification (RPA) assay was developed for rapid and accurate detection of Mycoplasma pneumoniae. The assay showed high sensitivity, fast amplification, ease of use, and no cross-reaction with other respiratory pathogens. It has the potential to serve as a promising test for early diagnosis of M. pneumoniae infection.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Chemistry, Analytical
Chang Liu, Xuechun Yao, Chunlong Liu, Shengping You, Wei Qi, Mengfan Wang
Summary: The study established and optimized two lateral flow device based molecular assay systems, RPA-NFO-LFT and RPA-Cas12a-LFT, for the detection of Candida albicans (C. albicans) infection. Both systems showed good specificity and sensitivity, with a lower detection limit of 10(3) CFU ml(-1). RPA-Cas12a-LFT demonstrated higher accuracy for visual interpretation and better stability towards samples with serum nucleic acid interference compared to the conventional qPCR method.
ANALYTICAL METHODS
(2023)
Article
Immunology
Shuang Liu, Siyuan Huang, Fang Li, Yuanyuan Sun, Jin Fu, Fei Xiao, Nan Jia, Xiaolan Huang, Chunrong Sun, Juan Zhou, Yi Wang, Dong Qu
Summary: The study developed a simple, rapid, sensitive, and specific detection platform for P. aeruginosa infection. The newly developed P. aeruginosa-CRISPR-RPA assay showed high sensitivity and specificity, and could complete P. aeruginosa detection within half an hour.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Chemistry, Analytical
Michihiko Nakano, Masafumi Inaba, Junya Suehiro
Summary: This study introduces a rapid and cost-effective method for amplicon detection, where amplicons are attached to magnetic microbeads, suspended in deionized water, and agglomerated in a circular pattern on a hydrophilic surface under a magnetic field, making them visible to the naked eye.
Article
Chemistry, Analytical
Kieu The Loan Trinh, Nae Yoon Lee
Summary: In this study, a wearable nucleic acid amplification microdevice was developed, which utilizes human body heat for reaction and provides an instrument-free solution for DNA amplification in resource-limited settings. The device is flexible, adhesive, and capable of rapid amplification.
Article
Chemistry, Multidisciplinary
Adrian Butterworth, Pratibha Pratibha, Andreas Marx, Damion K. Corrigan
Summary: This study demonstrates the development of a nucleic acid-based assay for antibiotic resistance genes through a modified RPA reaction and electrochemical measurement. Results show that important gene sequences can be detected by incorporating labeled nucleotides into amplified DNA strands using reagents from a standard RPA reaction kit.
Article
Plant Sciences
Felix Moran, Mario Herrero-Cervera, Sofia Carvajal-Rojas, Ester Marco-Noales
Summary: Huanglongbing (HLB) is a devastating disease that affects all commercial citrus species worldwide, and it is transmitted by psyllid vectors. This study presents a new real-time recombinase polymerase amplification (RPA) protocol that can detect the three bacteria species associated with HLB in both plant and insect samples. The protocol is accurate, sensitive, specific, and reliable, and it does not require specialized equipment, making it ideal for field use. The results indicate the potential of this new RPA protocol to be implemented as a reliable on-site detection kit for HLB.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Microbiology
Feina Li, Jing Xiao, Haiming Yang, Yao Yao, Jieqiong Li, Huiwen Zheng, Qian Guo, Xiaotong Wang, Yuying Chen, Yajie Guo, Yonghong Wang, Chen Shen
Summary: In this study, a rapid and efficient method utilizing RPA-CRISPR/Cas12a technology was proposed for the diagnosis of Mycoplasma pneumoniae. This method eliminates the need for expensive instruments and specialized personnel. The entire process, including sample extraction, RPA reaction, CRISPR/Cas12a detection, and visual observation, can be completed within 1 hour with high sensitivity and specificity.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Chemistry, Analytical
Yiran Xiao, Honglin Ren, Han Wang, Deying Zou, Yixin Liu, Haosong Li, Pan Hu, Yansong Li, Zengshan Liu, Shiying Lu
Summary: A low-cost platform based on the CRISPR/Cas12a system was designed for the rapid detection of Listeria monocytogenes. This method combines CRISPR/Cas12a and recombinase polymerase amplification (RPA) to provide a contamination-free platform for nucleic acid detection with high specificity and ultrasensitivity. The optimized method can specifically detect target DNA in 25 minutes and successfully detect LM in spiked pork samples and natural meat samples.
Article
Chemistry, Multidisciplinary
Gaihua Cao, Jiangbo Dong, Xiaolong Chen, Peng Lu, Yifan Xiong, Lan Peng, Jiawei Li, Danqun Huo, Changjun Hou
Summary: In this study, a strategy (MPT-Cas12a/13a) was developed that combines CRISPR/Cas12a and Cas13a for simultaneous detection of CaMV35S and T-nos. This strategy utilizes multiplex PCR and transcription to achieve simultaneous detection with different signals in the same space. The MPT-Cas12a/13a showed excellent sensitivity, with detection limits as low as 11 copies of T-nos and 13 copies of CaMV35S, and demonstrated outstanding specificity and anti-interference ability in actual sample analysis. Therefore, it has the potential to be used for the detection of GM crops.
CHEMICAL COMMUNICATIONS
(2022)
Article
Biophysics
Saurabh Tomar, Barbora Lavickova, Carlotta Guiducci
Summary: The application of recombinase polymerase amplification (RPA) for pH-based detection of DNA amplification has been explored, indicating that commercial RPA kits inherently buffer the pH. Strategies to minimize the buffering capacity of the RPA kit have been investigated, while maintaining amplification efficiency remains a challenge.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Tasnima Alam Asa, Pradeep Kumar, Jaehyeon Lee, Young Jun Seo
Summary: In this study, a new method for detecting SARS-CoV-2 virus was presented, which combines high selectivity and sensitivity through the integration of ligation and recombinase polymerase amplification processes. The method allows for efficient and rapid diagnosis of the virus-mediated disease within 30 minutes using colorimetric analysis. This robust, straightforward, highly selective, efficient, and ultrasensitive method has the potential for point-of-care analysis and can effectively detect other RNA-based diseases.
Article
Chemistry, Analytical
Rui Ge, Hanjie Dai, Shumin Zhang, Jie Wei, Tianhui Jiao, Qingmin Chen, Quansheng Chen, Xiaomei Chen
Summary: In this study, a photoelectrochemical (PEC) biosensor combined with recombinase polymerase amplification (RPA) technology (RPA-PEC) was proposed for rapid detection of multiple foodborne pathogens. The RPA was initiated on homemade three-dimensional screen-printed paper-based electrodes, forming a lab-on-paper platform. Using the DNA-PEC signaler, photocurrents were achieved after only 20 minutes of RPA, with superior detection performance compared to conventional agarose gel electrophoresis.
ANALYTICAL CHEMISTRY
(2023)
Review
Biochemical Research Methods
Xhensila Shkembi, Marketa Svobodova, Vasso Skouridou, Abdulaziz S. Bashammakh, Abdulrahman O. Alyoubi, Ciara K. O'Sullivan
Summary: This review discusses the selection of aptamers against mycotoxins and their incorporation into optical and electrochemical aptasensors. The strategies, advantages, and disadvantages of each approach are outlined, along with the different materials used and immobilization methods employed to achieve the highest sensitivity and selectivity.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Chemistry, Analytical
Sallam AL-Madhagi, Ciara K. O'Sullivan, Mamas I. Prodromidis, Ioanis Katakis
Summary: A reagent-less DNA sensor utilizing gold nanoparticles, modified primers, and isothermal amplification has been developed and successfully applied for the detection of Karlodinium armiger. The sensor demonstrates high sensitivity and rapid detection speed through optimized reaction conditions.
Article
Biotechnology & Applied Microbiology
Greta Gaiani, Anna Toldra, Karl B. Andree, Maria Rey, Jorge Diogene, Carles Alcaraz, Ciara K. O'Sullivan, Monica Campas
Summary: The study developed three molecular assays for detecting Gambierdiscus and Fukuyoa genera, as well as G. australes and G. excentricus species, achieving a remarkable limit of detection of a single cell. These assays provide a rapid and cost-effective strategy for reliable screening of samples and ciguatera risk assessment, ensuring seafood safety and protection of human health.
JOURNAL OF APPLIED PHYCOLOGY
(2021)
Article
Chemistry, Multidisciplinary
David Kodr, Cansu Pinar Yenice, Anna Simonova, Dijana Pavlovic Saftic, Radek Pohl, Veronika Sykorova, Mayreli Ortiz, Ludek Havran, Miroslav Fojta, Zbigniew J. Lesnikowski, Ciara K. O'Sullivan, Michal Hocek
Summary: A series of modified nucleoside triphosphates bearing dicarba-nido-undecaborate or metal bis(dicarbollide) groups were prepared and utilized for DNA synthesis and redox labeling. The combination of these redox labels allowed for the first fully orthogonal redox coding of all four canonical nucleobases, enabling determination of nucleobase composition of short DNA stretches with electrochemical readout in one simple primer extension experiment.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Biophysics
Mayreli Ortiz, Miriam Jauset-Rubio, David Kodr, Anna Simonova, Michal Hocek, Ciara K. O'Sullivan
Summary: This study developed an electrochemical platform for the detection of SNP in the MYH7 gene associated with hypertrophic cardiomyopathies using fingerprick blood samples. The platform uses solid-phase primer elongation and recombinase polymerase amplification to accurately identify SNPs, and only requires a small amount of fingerprick blood for analysis.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Environmental Sciences
Greta Gaiani, Francesca Cucchi, Anna Toldra, Karl B. Andree, Maria Rey, Takeshi Tsumuraya, Ciara K. O'Sullivan, Jorge Diogene, Monica Campas
Summary: Several genera of marine dinoflagellates are capable of producing bioactive compounds that can impact human health, with Gambierdiscus and Fukuyoa standing out for their production of toxins, including ciguatoxins. A multiplex detection system and rapid DNA extraction technique have been developed for identifying toxin-producing species, enabling swift detection in field samples.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Chemistry, Multidisciplinary
Jonathan Sabate del Rio, Hyun-Kyung Woo, Juhee Park, Hong Koo Ha, Jae-Ryong Kim, Yoon-Kyoung Cho
Summary: Electrochemical biosensors have the potential to be cost-effective diagnostic tools for point-of-care testing. However, the sensitivity of directly analyzing complex biological fluids such as plasma has been limited due to biofouling. This study demonstrates a method for creating nanostructured and nanoporous gold electrodes, which greatly increases the surface area and improves detection sensitivity. The surfactant-based electrochemical etch-deposit interplay for nanostructure/nanopore growth (SEEDING) process enhances the sensitivity and antibiofouling capabilities of biosensors, enabling direct analysis of tumor-derived extracellular vesicles in complex biofluids.
ADVANCED MATERIALS
(2022)
Article
Biochemical Research Methods
Xhensila Shkembi, Mary Luz Botero, Vasso Skouridou, Miriam Jauset-Rubio, Marketa Svobodova, Pablo Ballester, Abdulaziz S. Bashammakh, Mohammad S. El-Shahawi, Abdulrahman O. Alyoubi, Ciara K. O. Sullivan
Summary: The illicit use of AAS as performance-enhancing drugs poses a threat to both competitive sports and public health. Current detection methods are limited to laboratory settings and cannot be performed on-site. However, the development of aptamer technology offers the potential for simple and cost-effective on-site analysis. In this study, a label-free colorimetric assay was developed using aptamers to detect the abuse of AAS.
ANALYTICAL BIOCHEMISTRY
(2022)
Review
Engineering, Marine
Camila Barria, Piera Vasquez-Calderon, Catalina Lizama, Pablo Herrera, Anahi Canto, Pablo Conejeros, Orietta Beltrami, Benjamin A. Suarez-Isla, Daniel Carrasco, Ignacio Rubilar, Leonardo Guzman, L. Rene Duran, Doris Oliva
Summary: Harmful Algal Blooms (HABs) are a global problem that has been increasing in frequency in the Southeast Pacific region of Chile. The types of HABs in Chile include toxic HABs and ichthyotoxic HABs, with a lack of knowledge about harmful algal species in the Chilean Humboldt Current system. The occurrence and impacts of HAB events in Chile have been analyzed over a 65-year period, with diatoms, dinoflagellates, and silicoflagellates being the main causative microalgae species. The frequency of HAB events appears to be associated with monitoring efforts.
JOURNAL OF MARINE SCIENCE AND ENGINEERING
(2022)
Article
Chemistry, Analytical
Koena L. Moabelo, Teresa M. Lerga, Miriam Jauset-Rubio, Nicole R. S. Sibuyi, Ciara K. O'Sullivan, Mervin Meyer, Abram M. Madiehe
Summary: This study presents the development of a rapid aptasensor based on gold nanoparticles for the detection of RBP4. The method utilizes the specific binding of aptamers to RBP4 and the color change of gold nanoparticles to achieve rapid detection, with results available within 5 minutes.
Article
Chemistry, Analytical
Miriam Jauset-Rubio, Mayreli Ortiz, Ciara K. O'Sullivan
Summary: Aptamer-based assays and sensors show promise as alternatives to antibodies for their flexibility in assay formats. This study demonstrates the use of a biotin-modified aptamer in enhancing sensitivity and achieving lower detection limits in a colorimetric enzyme-linked oligonucleotide assay and electrochemical detection. The generic nature of this assay format allows for easy and cost-effective application to various aptamers.
Article
Chemistry, Analytical
Daniel Torregrosa, Miriam Jauset-Rubio, Raquel Serrano, Marketa Svobodov, Guillermo Grindlay, Ciara K. O'Sullivan, Juan Mora
Summary: The objective of this study is to evaluate different competitive aptamer assays for the detection of beta-conglutin in flour samples using inductively coupled plasma mass spectrometry (ICP-MS). Two competitive aptamer assay schemes were developed, one using thiolated aptamers on gold nanoparticles and the other using biotinylated aptamers linked to streptavidin-AuNPs. The influence of ICP-MS detection mode on assay performance was explored. The optimized condition achieved a LOD of 2 pM, significantly improving the LOD compared to previous studies.
ANALYTICA CHIMICA ACTA
(2023)
Article
Fisheries
Paulina Bustos, Carolina Figueroa, Barbara Cadiz, Tamara Santander, Brian Dixon, Jose A. Gallardo, Pablo Conejeros
Summary: Recently, it was found that Atlantic salmon vaccinated against Piscirickettsia salmonis lose their protection when co-infected with Caligus rogercresseyi. The reasons for this effect and the molecular basis of the immune responses in vaccinated salmon during C. rogercresseyi infection have not been determined. In this study, changes in immune gene expression were investigated in vaccinated Atlantic salmon co-infected with C. rogercresseyi and P. salmonis. The results showed that the co-infection had a similar effect on immune gene expression as a single P. salmonis infection, suggesting a decreased cellular response. However, some individual fish exhibited a significantly higher immune response, indicating variation in vaccine efficacy.
JOURNAL OF FISH DISEASES
(2023)
Article
Chemistry, Multidisciplinary
Hye-Jin Jeong, Ji-Hyeon Park, Joo H. H. Kang, Jonathan Sabate del Rio, Seong-Ho Kong, Tae-Eun Park
Summary: A human stomach micro-physiological system (hsMPS) combining organoid and MPS technology is described. The system enables the functional maturation of gastric epithelial cells and the recreation of a mesh-like mucus layer and well-developed epithelial junctional complexes. It successfully demonstrates gastroprotection mechanisms against Helicobacter pylori.