期刊
MARINE DRUGS
卷 17, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/md17030185
关键词
chondroitinase; eukaryotic expression; enzymatic properties; oligosaccharides
资金
- National Natural Science Fund of China [31872893]
- Key Research and Development Project Foundation of Shandong Province [2017YYSP003]
- Shandong Natural Science Fund [ZR2017MD006]
Chondroitinase (ChSase), a type of glycosaminoglycan (GAG) lyase, can degrade chondroitin sulfate (CS) to unsaturate oligosaccharides, with various functional activities. In this study, ChSase AC II from a newly isolated marine bacterium Arthrobacter sp. CS01 was cloned, expressed in Pichia pastoris X33, purified, and characterized. ChSase AC II, with a molecular weight of approximately 100 kDa and a specific activity of 18.7 U/mg, showed the highest activity at 37 degrees C and pH 6.5 and maintained stability at a broad range of pH (5-7.5) and temperature (below 35 degrees C). The enzyme activity was increased in the presence of Mn2+ and was strongly inhibited by Hg2+. Moreover, the kinetic parameters of ChSase AC II against CS-A, CS-C, and HA were determined. TLC and ESI-MS analysis of the degradation products indicated that ChSase AC II displayed an exolytic action mode and completely hydrolyzed three substrates into oligosaccharides with low degrees of polymerization (DPs). All these features make ChSase AC II a promising candidate for the full use of GAG to produce oligosaccharides.
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