Article
Biophysics
Anton Turaev, Ekaterina A. Isaakova, Vjacheslav V. Severov, Alexandra N. Bogomazova, Timofei S. Zatsepin, Makar Sardushkin, Andrey Aralov, Maria A. Lagarkova, Galina E. Pozmogova, Anna M. Varizhuk
Summary: The design of robust sensors for measuring intracellular pH based on native DNA i-motifs offers a sensitive and fast-response approach without the need for additional modules. This 'genome-inspired' design can be used to monitor proton dynamics in the nucleus, suggesting it is a productive approach for the development of biocompatible molecular tools.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Multidisciplinary
Yuwei Hu, Yujie Ke, Itamar Willner
Summary: A cascaded DNA hydrogel governed by pH-configurable A-motif, i-motif, and T·A-T triplex configurations is presented. Different pH conditions dictate the formation and stability of the hydrogel through the protonation and deprotonation of adenine and cytosine residues. This study advances the development of smart hydrogels capable of adapting to various environmental conditions by introducing multiple pH-responsiveness.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Chemistry, Analytical
Ziwen Tao, Guotao Yi, Chao Zeng, Qianfen Zhuang, Yong Wang
Summary: In this study, a simple, sensitive, and selective approach for detecting silver ions was developed using silver nanoclusters. The phosphorescence of the nanoclusters was quenched upon the addition of silver ions, and the quenching ratio was linearly proportional to the concentration of silver ions. The method exhibited high selectivity and was successfully applied to detect silver ions in lake water samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Materials Science, Multidisciplinary
Yuwei Hu, Jackie Y. Ying
Summary: Smart DNA hydrogels containing dynamic reconfigurable DNA crosslinking units exhibit sol-gel transition, shape memory, self-healing, sensing, cargo encapsulation, and delivery. They are designed to respond to external stimuli such as light, pH, ions, fuel strands, thermal triggers, chemical, and biocatalytic reactions for various applications in material science, chemistry, and biomedicine. This review focuses on the recent advances in pH-sensitive nucleic acid structures, particularly A-motif, i-motif, and triplex, for pH-responsive DNA hydrogels. The review discusses the structural details of pH-triggered A-motif, i-motif, and triplex configurations as the foundation for constructing pH-responsive DNA hydrogels, as well as the physicochemical properties and applications of these hydrogels in shape modulation, self-healing, drug release, sensing, therapy, and soft matter.
Article
Chemistry, Analytical
Ziwen Tao, Guotao Yi, Chao Zeng, Qianfen Zhuang, Yong Wang
Summary: Silver ion (Ag+) is a highly toxic heavy metal pollutant, and a sensitive and selective method for its detection is urgently needed for environmental protection. Water-soluble red-emitting micellized phosphorescent silver nanoclusters have been developed as probes for the rapid and simple detection of Ag+, exhibiting high selectivity and good recoveries in lake water samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Multidisciplinary
Nataliia A. Petrunina, Alina S. Shtork, Maria M. Lukina, Vladimir B. Tsvetkov, Yuri M. Khodarovich, Alexey V. Feofanov, Anastasia M. Moysenovich, Eugene G. Maksimov, Victoria O. Shipunova, Timofei S. Zatsepin, Alexandra N. Bogomazova, Victoria O. Shender, Andrey V. Aralov, Maria A. Lagarkova, Anna M. Varizhuk
Summary: DNA-intercalated motifs serve as convenient scaffolds for designing pH-responsive nanomachines, including biocompatible pH sensors. By using a simple unimolecular dual-labeled iM scaffold and modifying it with abasic or alkyl linkers, the thermal stability and pH-induced conformational transitions of the iM were improved.
Article
Chemistry, Multidisciplinary
Shiliang He, Mengmeng Liu, Fangfei Yin, Jiangbo Liu, Zhilei Ge, Fan Li, Min Li, Jiye Shi, Lihua Wang, Xiuhai Mao, Xiaolei Zuo, Qian Li
Summary: The response sensitivity of a molecular sensor depends on the folding cooperativity of its responsive module. This can be enhanced through preorganization, as demonstrated with an H+-responsive dimeric DNA i-motif, to create robust intracellular pH sensors with high response sensitivity.
CHEMICAL COMMUNICATIONS
(2021)
Article
Chemistry, Analytical
Johnson Dalmieda, Ana Zubiarrain-Laserna, Devanjith Ganepola, P. Ravi Selvaganapathy, Peter Kruse
Summary: Silver is commonly used for water disinfection in hospitals and purifiers. Excessive consumption could lead to adverse effects. A new silver sensor utilizing few-layer graphene networks for detection within a concentration range of 3 ppb to 1 ppm has been developed, which can be reset and reused multiple times, demonstrating a potential pathway for cation sensing in various water sources.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Biochemistry & Molecular Biology
Golam Mustafa, Prabesh Gyawali, Jacob A. Taylor, Parastoo Maleki, Marlon V. Nunez, Michael C. Guntrum, Sajad Shiekh, Hamza Balci
Summary: This paper presents a collection of single molecule studies on the i-motif structure formed by the human telomeric sequence. The i-motif structure has recently gained attention as a physiologically relevant structure and a pH sensor. The study establishes single molecule Forster resonance energy transfer (smFRET) as a tool to investigate the i-motif under different pH and ionic conditions, revealing the pH and salt dependence of i-motif formation and capturing the intermediate folding states in real time. Furthermore, the i-motif is demonstrated to be a potential in-situ pH sensor.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Spectroscopy
Tong Yang, Shuzhen Peng, Ruidi Zeng, Qiuda Xu, Xiong Zheng, Dandan Wang, Xiaoshun Zhou, Yong Shao
Summary: This study developed a visible light-driven DNAzyme based on human telomeric i-motifs, using hypericin as the cofactor and dissolved oxygen as the oxidant source. The DNAzyme has broad applications and its catalytic performance can be improved using a long-lived mediator.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Physical
Hui-Min Zeng, Bo-Xing Jin, Wei-Hong Wu, Chao Wang, Zhan-Guo Jiang, Zhigang Jin, Cai-Hong Zhan
Summary: The controllable construction of pure inorganic polyoxometalate (POM) zite materials with well-defined secondary building units (SBUs) is challenging. In this work, three distinct POM porous framework compounds 1-3 based on specific SBUs and transition-metal Ag linkers were self-assembled, providing valuable experience for synthetic chemists to obtain more interesting materials. Compound 1-3 exhibit cell type-specific, dose-dependent, and pH-sensitive anticancer behavior, making them promising anticancer agents for lung cancer therapy.
CHEMISTRY OF MATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Mingpan Cheng, Dehui Qiu, Liezel Tamon, Eva Istvankova, Pavlina Viskova, Samir Amrane, Aurore Guedin, Jielin Chen, Laurent Lacroix, Huangxian Ju, Lukas Trantirek, Aleksandr B. Sahakyan, Jun Zhou, Jean-Louis Mergny
Summary: Recent studies have shown that i-DNA, a four-stranded cytosine-rich DNA known as the i-motif, is formed in vivo. This study tested 271 different sequences and found that i-DNA stability is nearly independent on total spacer length, with the central spacer playing a special role in stability. The study also revealed that stability depends on the length of the C-tracts at both acidic and neutral pHs.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Analytical
G. Martinez-Saucedo, F. M. Cuevas-Muniz, R. Sanchez-Fraga, I. Mejia, J. J. Alcantar-Pena, I. R. Chavez-Urbiola
Summary: By using a cellulose microfluidic pH boosting layer, a non-enzymatic copper oxide glucose sensor strip is adapted for neutral pH samples. This adaptation allows the non-enzymatic technology to measure glucose in situ. A three-electrode system is constructed to test samples in an electrochemical cell and a sensing strip. The system shows the capability to measure glucose in neutral pH samples and human blood.
Article
Food Science & Technology
Yuan Fang, Shusen Ding, Weiran Li, Jingjing Zhang, Hui Sun, Xiaodong Lin
Summary: In this study, a dual-channel nanosensor was developed for the detection of Ag+ using synthesized nanomaterials with specific response to Ag+. The sensor combines fluorescence and colorimetric analysis to improve the sensitivity and accuracy of detection, and has shown satisfactory results in real samples.
Review
Chemistry, Multidisciplinary
Liviu Sacarescu, Andreea-Laura Chibac-Scutaru, Gheorghe Roman, Gabriela Sacarescu, Mihaela Simionescu
Summary: This article reviews the synthesis and properties of pyrazolines as fluorescent sensors, as well as their capabilities in cellular detection and monitoring of metal ions, sulfites, multi-ionic mixtures, pH, and glutathione. The metals analyzed include zinc, copper, iron, nickel, mercury, aluminum, and calcium.
ENVIRONMENTAL CHEMISTRY LETTERS
(2023)
Article
Spectroscopy
Anna Dembska, Bernard Juskowiak
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2015)
Article
Biochemistry & Molecular Biology
Angelika Switalska, Ryszard Kierzek, Anna Dembska, Bernard Juskowiak
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2017)
Article
Chemistry, Analytical
Angelika Switalska, Anna Dembska, Agnieszka Fedoruk-Wyszomirska, Bernard Juskowiak
Article
Biochemical Research Methods
Anna Dembska, Patrycja Rzepecka, Bernard Juskowiak
JOURNAL OF FLUORESCENCE
(2013)
Article
Chemistry, Physical
Sanchari Chowdhury, Zhikun Wu, Andrea Jaquins-Gerstl, Shengpeng Liu, Anna Dembska, Bruce A. Armitage, Rongchao Jin, Linda A. Peteanu
JOURNAL OF PHYSICAL CHEMISTRY C
(2011)
Article
Biochemistry & Molecular Biology
Patrycja Bielecka, Anna Dembska, Bernard Juskowiak
Article
Multidisciplinary Sciences
Anna Dembska, Angelika Switalska, Agnieszka Fedoruk-Wyszomirska, Bernard Juskowiak
SCIENTIFIC REPORTS
(2020)
Article
Chemistry, Physical
Anna Dembska, Julia Masternak, Martina Prochota
JOURNAL OF MOLECULAR STRUCTURE
(2020)
Article
Biochemistry & Molecular Biology
Joanna Nowak-Karnowska, Agata Gluszylska, Joanna Kosman, Grazyna Neunert, Anna Dembska
Summary: The highly fluorescent 9-methoxyluminarine ligand 9-MeLM was found to interact with different topologies of G-quadruplexes, selectively quenched by parallel c-MYC G-quadruplex DNA. This suggests that 9-MeLM can potentially be used as a selective probe for parallel G-quadruplexes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Analytical
Patrycja Filipczuk, Angelika Switalska, Joanna Kosman, Grzegorz Nowaczyk, Anna Dembska
Summary: The study found that Tel22C12-AgNCs and TBAC12-AgNCs can serve as fluorescent probes, exhibiting different fluorescence quenching effects when binding K+ or Na+ ions. Tel22C12-AgNCs showed a higher binding preference for K+ ions and hold potential for monitoring transmembrane potassium transport.
Article
Chemistry, Multidisciplinary
Sylwia Studzinska, Magdalena Skoczylas, Szymon Bocian, Anna Dembska, Boguslaw Buszewski
Review
Chemistry, Analytical
Anna Dembska, Patrycja Bielecka, Bernard Juskowiak
ANALYTICAL METHODS
(2017)
Article
Chemistry, Analytical
Anna Dembska, Elzbieta Kierzek, Bernard Juskowiak
ANALYTICA CHIMICA ACTA
(2017)
Article
Chemistry, Analytical
Anna Dembska, Patrycja Rzepecka, Bernard Juskowiak
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)