Review
Chemistry, Applied
Beatriz del Rio, Maria Fernandez, Begona Redruello, Victor Ladero, Miguel A. Alvarez
Summary: Biogenic amines (BA) are molecules with biological functions that can accumulate at toxic concentrations in foods. Some microorganisms have been identified as responsible for their accumulation. Histamine, tyramine, and putrescine are the most commonly found BA at highest concentrations. Ingesting food with high BA concentrations can lead to intoxication. Additionally, there is synergy between different BA, emphasizing the need for regulation to protect consumer health.
Review
Food Science & Technology
Maria Schirone, Luigi Esposito, Federica D'Onofrio, Pierina Visciano, Maria Martuscelli, Dino Mastrocola, Antonello Paparella
Summary: This review analyzes the presence and role of biogenic amines in meat and meat products, as well as the effect of technological conditions. The provided data can be useful in developing solutions for controlling biogenic amines formation and protecting perishable products.
Article
Microbiology
Salvador Hernandez-Macias, Alba Martin-Garcia, Nuria Ferrer-Bustins, Oriol Comas-Baste, Montserrat Riu-Aumatell, Elvira Lopez-Tamames, Anna Jofre, M. Luz Latorre-Moratalla, Sara Bover-Cid, M. Carmen Vidal-Carou
Summary: The use of cava lees and their phenolic extract have been shown to be effective strategies in controlling the formation of biogenic amines in fermented products, particularly in fermented sausages.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Food Science & Technology
Marta Moniente, Laura Botello-Morte, Diego Garcia-Gonzalo, Raquel Virto, Rafael Pagan, Vicente Ferreira, Ignacio Ontanon
Summary: A method for the quantitative determination of four biogenic amines in different dairy products has been developed and applied to milk, yogurt, and kefir samples. Significant levels of biogenic amines were found in yogurt and kefir samples, with values reaching up to 79 mg/kg in kefir samples. While these levels are not a cause for concern for the general population, they are important to be known by individuals sensitive to biogenic amines.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Biotechnology & Applied Microbiology
Lucas Kettner, Carina Braun, Ines Seitl, Eva Pross, Lutz Fischer
Summary: In this study, a putative diamine oxidase (DAO) from Yarrowia lipolytica was successfully recombinantly integrated into the genome of Y. lipolytica for production in a bioreactor, showing a 93-fold increase in specific DAO activity compared to the native production. The produced DAO-1 exhibited broad substrate selectivity, with tyramine, histamine, putrescine, and cadaverine being the favored substrates, making it a potentially valuable enzyme for biogenic amine degradation in the food industry.
JOURNAL OF BIOTECHNOLOGY
(2021)
Article
Chemistry, Applied
Valterney L. Deus, Lais M. Resende, Eliete S. Bispo, Adriana S. Franca, Maria Beatriz A. Gloria
Summary: Cloning techniques have been used to improve the agronomic traits of nine fine cocoa varieties, resulting in variations in the concentrations of bioactive amines, phenolic compounds, and antioxidant potential of dark monoclonal chocolates. The chemometric models based on FTIR data provided satisfactory predictions for the concentrations of amines, phenolic compounds, and antioxidant activity, confirming the significant contribution of amines to the antioxidant potential of chocolates. The analytical method (DPPH vs. Rancimat) also affected the results of antioxidant activity in the chocolates.
Article
Food Science & Technology
Agustina Sarquis, Diellza Bajrami, Boris Mizaikoff, Victor Ladero, Miguel A. Alvarez, Maria Fernandez
Summary: The aim of this study was to investigate the biofilm-producing capacity of Lentilactobacillus parabuchneri strains. The results showed that the biofilm matrices of these strains were mainly composed of proteins and they easily adhered to beech wood. This study provides important insights into the biofilm-forming capacity of histamine-producing L. parabuchneri strains and how to prevent their formation for improving food safety.
Article
Biochemistry & Molecular Biology
Dimitrios Tsikas, Bibiana Beckmann, Svetlana Baskal, Gorig Brunner
Summary: Gas chromatography-mass spectrometry (GC-MS) is a useful method for quantitative determination of polyamines spermidine (SPD), putrescine (PUT), and biogenic amine agmatine (AGM) in biological samples. However, GC-MS analysis of biogenic amine histamine (HA) is challenging and less efficient. This study investigated the derivatization of HA and the effects of toluene, ethyl acetate, and oven temperature program on HA analysis. The simultaneous quantitative analysis of HA, AGM, PUT, and SPD requires the use of ethyl acetate for extraction and injection into GC-MS apparatus, and a starting column temperature of 40°C.
Article
Food Science & Technology
Pierina Visciano, Maria Schirone
Summary: The formation of biogenic amines in food and beverages is mainly due to the presence of proteins and/or free amino acids that act as substrates for microbial or natural enzymes. Fermentation, present in alcoholic beverages, coffee, and tea, significantly affects their production. Some biogenic amines can also be naturally found in fruit juices or fruit-based drinks. To prevent adverse effects, it is important to select lactic acid bacteria with low decarboxylating activity or implement good manufacturing practices to hinder biogenic amine production.
Article
Electrochemistry
Mingxiang Meng, Min Lin
Summary: This study developed an electroanalytical sensor using a polypyrrole-gold composite for detecting tyramine in fermented foods. The sensor demonstrated high sensitivity and selectivity, as well as stability and reproducibility under ambient conditions. The practical application of the sensor showed its potential for routine use.
INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE
(2023)
Article
Nutrition & Dietetics
Sonia Sanchez-Perez, Oriol Comas-Baste, M. Teresa Veciana-Nogues, M. Luz Latorre-Moratalla, M. Carmen Vidal-Carou
Summary: A low-histamine diet is currently the most advised strategy for preventing the symptoms of histamine intolerance. However, there is great heterogeneity in the type of excluded foods among different studies, with fermented foods being the only unanimous exclusion. Approximately 32% of excluded foods have high histamine content as a likely explanation.
Article
Food Science & Technology
Yali Huang, Honghong Yu, Shiling Lu, Liangjun Zou, Zonggui Tang, Tao Zeng, Juan Tang
Summary: The inclusion complexes of ferulic acid were found to significantly reduce tyramine accumulation in traditional smoked horsemeat sausages by inhibiting the growth and gene expression of Enterobacteriaceae, demonstrating antibacterial properties.
Article
Biochemistry & Molecular Biology
Marzena Pawul-Gruba, Tomasz Kiljanek, Anna Madejska, Jacek Osek
Summary: A method for determining eight biogenic amines in ripened cheeses was developed and validated using HPLC-DAD, showing high accuracy and precision.
Article
Biochemistry & Molecular Biology
Yun Ai, Yan Ni Sun, Li Liu, Fang Yuan Yao, Yan Zhang, Feng Yi Guo, Wen Jie Zhao, Jian Li Liu, Ning Zhang
Summary: This study successfully characterized biogenic amines in different parts of Lycium barbarum L. using HPLC, revealing four different types of amines. The bark had the highest histamine content, while the flower had the highest putrescine content. There were significant differences in biogenic amine content among different parts of Lycium barbarum L., which should be considered when further utilizing them.
Article
Nutrition & Dietetics
Lucas Kettner, Ines Seitl, Lutz Fischer
Summary: A new diamine oxidase (DAO-1) was discovered in yeast, which has the potential to treat histamine intolerance by oral supplementation under simulated intestinal conditions.
Article
Food Science & Technology
Marta Gallardo-Fernandez, Ana B. Cerezo, Ruth Hornedo-Ortega, Ana M. Troncoso, M. Carmen Garcia-Parrilla
Summary: This study revealed the molecular mechanism underlying the inhibition of VEGF signaling by hydroxytyrosol metabolites and indolic compounds, and established a relationship between their structure and bioactivity. The compounds exhibited anti-angiogenic effects by regulating signaling pathways such as PLC gamma 1 and eNOS.
Article
Chemistry, Applied
M. Gallardo-Fernandez, J. Valls-Fonayet, E. Valero, R. Hornedo-Ortega, T. Richard, A. M. Troncoso, M. C. Garcia-Parrilla
Summary: Yeasts have the ability to synthetise bioactive compounds like Melatonin, Serotonin and Hydroxytyrosol, and research has identified at least two pathways involved in their biosynthesis. Understanding these mechanisms can help enhance the bioactivity potential of fermented beverages.
Article
Nutrition & Dietetics
Marina Gonzalez-Ramirez, Rocio Sanchez-Carrera, Angela Cejudo-Lopez, Mauricio Lozano-Navarrete, Elena Salamero Sanchez-Gabriel, M. Alfonso Torres-Bengoa, Manuel Segura-Balbuena, Maria J. Sanchez-Cordero, Mercedes Barroso-Vazquez, Francisco J. Perez-Barba, Ana M. Troncoso, M. Carmen Garcia-Parrilla, Ana B. Cerezo
Summary: This study evaluated the impact of the SAlBi educa nutrition app in primary healthcare dietary counseling. The intervention group, who used the app and attended meetings, showed increased carbohydrate intake and decreased total fat intake after 4 weeks, as well as significant differences in dietary profiles during the COVID-19 pandemic.
Article
Nutrition & Dietetics
Marina Gonzalez-Ramirez, Angela Cejudo-Lopez, Mauricio Lozano-Navarrete, Elena Salamero Sanchez-Gabriel, M. Alfonso Torres-Bengoa, Manuel Segura-Balbuena, Maria J. Sanchez-Cordero, Mercedes Barroso-Vazquez, Francisco J. Perez-Barba, Ana M. Troncoso, M. Carmen Garcia-Parrilla, Ana B. Cerezo
Summary: This study designed and evaluated a tailored nutrition application to promote healthy eating habits, based on input from professionals in healthcare, nutrition, food science, and computer science. The app was developed with a reference model from scientific literature, and tested for usability through focus groups and a pilot test.
FRONTIERS IN NUTRITION
(2022)
Article
Biochemistry & Molecular Biology
Ines Mansinhos, Sandra Goncalves, Raquel Rodriguez-Solana, Hugo Duarte, Jose Luis Ordonez-Diaz, Jose Manuel Moreno-Rojas, Anabela Romano
Summary: The study examined the effects of drought stress induced by PEG on Thymus lotocephalus shoot cultures. It was found that PEG caused oxidative stress and reduced growth, photosynthetic pigment contents, and phenolic compounds production. Iron oxide nanoparticles, salicylic acid, and MeJA were identified as effective agents in alleviating drought stress. MeJA showed the most significant potential in protecting cells from oxidative damage and improving antioxidant activity.
Review
Food Science & Technology
Marta Gallardo-Fernandez, Marina Gonzalez-Ramirez, Ana B. Cerezo, Ana M. Troncoso, M. Carmen Garcia-Parrilla
Summary: This study evaluated the dietary intake of hydroxytyrosol (HT) in the European population by compiling scattered literature data. Olives, olive oil, and wine were identified as the main sources of HT. The estimated average intake varied among EU countries, with Greece showing the highest intake and Austria the lowest. The low estimated intake suggests that the use of HT as a food ingredient is feasible and offers new possibilities for utilizing waste products from olive oil and olive production.
Article
Food Science & Technology
Marta Gallardo-Fernandez, Ruth Hornedo-Ortega, Ana B. Cerezo, Ana M. Troncoso, M. Carmen Garcia-Parrilla
Summary: This study investigates the capacity of the metabolites DOPAL, MOPET, and MOPAL to prevent the aggregation and toxic effects of alpha syn fibrils. The results show that DOPAL exerts the greatest effect, preventing aggregation and alpha syn-induced neurotoxicity, and has a potent destabilising effect on the alpha syn fibrils. In addition, DOPAL can counteract the toxicity induced by alpha syn, possibly through the modulation of SIRT-2 and Hsp70.
FOOD AND CHEMICAL TOXICOLOGY
(2023)
Article
Chemistry, Applied
Monica Sanchez-Parra, Annalaura Lopez, Jose Manuel Munoz-Redondo, Jose Carlos Montenegro-Gomez, Jesus Perez-Aparicio, Gema Pereira-Caro, Raquel Rodriguez-Solana, Jose Manuel Moreno-Rojas, Jose Luis Ordonez-Diaz
Summary: The present study focused on the biogenic amine and volatile compound profiles of fresh yellowfin tuna and the resulting dry-cured product. The results showed that storage temperature and fishing season had limited effects on the quality and food safety features of the products, in accordance with EU regulations. The study also identified a wide variety of volatile compounds, with storage temperature affecting the concentrations of these compounds.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2022)
Article
Plant Sciences
Ines Mansinhos, Sandra Goncalves, Raquel Rodriguez-Solana, Jose Luis Ordonez-Diaz, Jose Manuel Moreno-Rojas, Anabela Romano
Summary: This study investigated the effects of different temperatures on two Mediterranean aromatic plants and found that temperature has different impacts on the primary and secondary metabolism of these plants. The osmoprotectant response also varies at different temperatures.
Article
Biochemistry & Molecular Biology
Isabel Velasco-Ruiz, Elsy De Santiago, Jose Luis Ordonez-Diaz, Gema Pereira-Caro, Jose Manuel Moreno-Rojas
Summary: The impact of in vitro gastrointestinal digestion and colonic fermentation on polyphenol compounds from different varieties of pistachio was evaluated by UHPLC-HRMS analysis. The total polyphenol content significantly decreased during oral and gastric digestion, while there were no significant changes after the intestinal phase. In vitro digestion revealed that hydroxybenzoic acids and flavan-3-ols were the main compounds in pistachios. Colonic fermentation affected the total phenolic content, and several catabolites were identified after fecal fermentation. These catabolites may contribute to the health properties attributed to pistachio consumption.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Food Science & Technology
Miguel Vega-Castellote, Dolores Perez-Marin, Irina Torres-Rodriguez, Jose-Manuel Moreno-Rojas, Jose-Luis Ordonez-Diaz, Maria-Teresa Sanchez
Summary: Currently, consumers demand supermarkets to use non-invasive analysis techniques to measure the sweetness of fruits like watermelon, and show increasing interest in knowing the nutritional quality. Near infrared spectroscopy (NIRS) was used to assess the physicochemical and nutritional quality of half-watermelons. The results demonstrate the viability of using NIRS in dynamic mode at the supermarket level to measure the sweetness of fruits and pave the way for nutritional labelling.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Monica Sanchez-Parra, Jose L. Ordonez-Diaz, Jesus Perez-Aparicio, Jose M. Moreno-Rojas
Summary: Dry-cured tuna, known as mojama in Spain, undergoes a traditional salting-drying process that brings changes to its physicochemical, biochemical, and microbiological parameters. The processing steps affect the physicochemical parameters, except for total acidity and pH. Water activity value and relative moisture percentage decrease, while water-holding capacity increases during salting and drying. Lipid oxidation and total volatile basic nitrogen values increase, indicating changes in flavor and protein degradation. Microbiological analysis confirms that the dry-cured tuna meets the regulation standards for dry-cured fish products.
APPLIED SCIENCES-BASEL
(2023)
Article
Food Science & Technology
Jose Manuel Moreno-Rojas, Cyntia Yadira Erazo Solorzano, Diego Armando Tuarez Garcia, Gema Pereira-Caro, Jose Luis Ordonez Diaz, Jose Manuel Munoz-Redondo, Raquel Rodriguez-Solana
Summary: Pre-drying cocoa before fermentation can improve the quality of cocoa. This study analyzed volatile compounds in Ecuadorian cocoa samples using GC-MS. The pre-drying process resulted in varietal homogenization and reduced fermentation time, making it a cost-effective and attractive option for farmers. Furthermore, fine-flavour varieties showed a clear improvement in aromatic quality with higher levels of compounds imparting positive notes after pre-drying.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Chemistry, Analytical
Yujia Ying, Huilin Li
Summary: Enzymes play a crucial role as biological catalysts in accelerating biochemical reactions in living organisms. This study proposes a convenient method for monitoring enzymatic catalytic processes using native top-down mass spectrometry. By exploring the heterogeneity of the chymotrypsin sample and using tandem mass spectrometry, the researchers were able to monitor covalent and noncovalent enzymatic complexes, substrates, and products during catalysis. The results demonstrate that this method has the potential to be a promising tool for characterizing biocatalysts.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Kang Yang, Shuaibo Shi, Jinyu Wu, Shaolong Han, Shengdi Tai, Shishen Zhang, Kun Zhang
Summary: H2O and D2O are important analogues closely related to various industries and monitoring fields. This study successfully distinguishes and detects H2O and D2O by designing a novel eu(III)-macrocycle with dual emitters, Eu-2a, and conducting fluorescence titration experiments.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Julia Kuligowski, Alvaro Perez-Rubio, Marta Moreno-Torres, Polina Soluyanova, Judith Perez-Rojas, Ivan Rienda, David Perez-Guaita, Eugenia Pareja, Ramon Trullenque-Juan, Jose Castell, Marcha Verheijen, Florian Caiment, Ramiro Jover, Guillermo Quintas
Summary: This study introduces a novel variable selection approach called cluster PLS (c-PLS) to assess the joint impact of variable groups selected based on biological characteristics on the predictive performance of a multivariate model. The usefulness of c-PLS is shown using miRNomic and metabolomic datasets obtained from the analysis of liver tissue biopsies.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Meixue Lai, Lijie Zhong, Siyi Liu, Yitian Tang, Tingting Han, Huali Deng, Yu Bao, Yingming Ma, Wei Wang, Li Niu, Shiyu Gan
Summary: This study presents a method for constructing wearable sweat electrolyte sensors using carbon fiber-based solid-contact ion-selective electrodes (SC-ISEs). By using carbon fibers extracted from commercial cloth as electrode material, the cost and reproducibility issues of flexible SC-ISEs were addressed. The results showed that the carbon fiber-based SC-ISEs exhibited reversible voltammetric and stable impedance performances, and had high reproducibility of standard potentials between normal and bending states.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Yang, Ying Liu, Min Deng, Peipei Wang, Dan Cheng, Songjiao Li, Longwei He
Summary: A near-infrared fluorescent probe has been developed to accurately measure the levels of peroxynitrite (ONOO-) in the endoplasmic reticulum (ER) in acute lung injury (ALI). The probe demonstrated rapid response, high selectivity, good sensitivity, and enhanced fluorescence intensity in response to ONOO-. It was successfully used to detect changes in ONOO- levels and showed significant increases in an ALI cell model and an ALI mouse model.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yanping Wang, Yuemei Chen, Kejun Li, Jinrong Zhou, Xin Yuan, Mei Zhang, Ke Huang
Summary: In this work, a portable analytical system based on point discharge chemical vapor generation atomic emission spectrometry (PD-CVG-AES) coupling with gold filament enrichment was designed. The highly sensitive analysis of Hg2+ indirectly realized the detection of ascorbic acid (AA). The measurement is based on the fact that Ag+ can decrease the concentration of Hg2+ by forming Ag-Hg amalgam in the presence of the reductant SnCl2, while AA can pre-reduce Ag+ to Ag0, leading to the generation of silver nanoparticles (Ag NPs). The developed novel analytical strategy broadens the application of microplasma-based AES and offers a higher level of sensitivity compared to current AA detection techniques.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yundi Huang, Bo Song, Kaiwen Chen, Deshu Kong, Jingli Yuan
Summary: This study developed two lysosome-targetable background-free TGL probes for efficient and accurate detection of 1O2, which can be used for monitoring endogenous 1O2 concentrations in lysosomes and discriminating variability induced by different photosensitizers. Furthermore, a smart luminescent sensor film was successfully prepared for on-site 1O2 production detection during PDT processes, offering a promising clinical monitoring tool for skin diseases.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Wei Lang, Jia-Mei Qin, Qian-Yong Cao
Summary: A novel polymer-based probe P1 was successfully synthesized for fluorescently ratiometric sensing of H2S with high selectivity and sensitivity. A smartphone sensing platform was constructed to conduct visual quantitative detection of H2S. P1 can be employed in evaluating the level fluctuations of H2S in living cells, testing water samples/wine samples, and monitoring food freshness.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Yuanyuan Qin, Shuda Liu, Shuyun Meng, Dong Liu, Tianyan You
Summary: The study developed a split aptamer-based sandwich-type ratiometric biosensor for the detection of 17 beta-estradiol (E2) using photoelectrochemical and electrochemical assays. The biosensor utilized split aptamer fragments to recognize E2 and trigger a hybridization chain reaction, resulting in the production of double-stranded DNA labeled with CdTe quantum dots. This DNA complex was able to sensitize CdTe quantum dots and generate response signals for E2 detection. The developed biosensor demonstrated high sensitivity and accuracy with two linear ranges and low detection limits.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Siriwan Teepoo, Jongjit Jantra, Khaunnapa Panapong, David Taiwo Ajayi
Summary: A novel immunochromatographic assay based on hyperbranched Au plasmonic blackbodies with a smartphone readout was developed for rapid and sensitive detection of leucomalachite green residues in fish and shrimp products.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Andrea L. Larraga-Urdaz, Borja Moreira-Alvarez, Jorge Ruiz Encinar, Jose M. Costa-Fernandez, Maria Luisa Fernandez-Sanchez
Summary: A major challenge in the 21st century is the development of point-of-care diagnostic tools. In this study, a highly sensitive and simple bioassay using AuNPs and MNAzymes was developed for rapid detection and quantification of miRNA-4739. The proposed strategy shows potential for breast cancer diagnosis.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Xiaohan Zhao, Anyu Wang, Lingzi Zhai, Jiuhe Gao, Sizhe Lyu, Yingshan Jiang, Tian Zhong, Ying Xiao, Xi Yu
Summary: A novel method using polystyrene-coated magnetic nanoparticles for extracting monohydroxy polycyclic aromatic hydrocarbons from urine samples was investigated. The proposed method is simple, sensitive, and efficient, with desirable sensitivity for analyzing low-abundance metabolites in large volumes of complex urine samples.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Ke Quan, Yuqing Zeng, Wenke Zhang, Fengfeng Li, Mengjiao Li, Zhihe Qing, Linlin Wu
Summary: In this study, a visual and reusable biosensor was developed for detecting substrates that are closely associated with human physiological health. The immobilized oxidase showed higher stability and sensitivity under harsh conditions, enabling reliable detection of substrates in complex fluids.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Dongjuan Wang, Xiuqian Ding, Jinling Xie, Juan Wang, Guanhao Li, Xin Zhou
Summary: In this study, a three-in-one sensor was developed for real-time detection of biogenic amines (BAs) with high sensitivity and selectivity. The sensor showed multimodal responses and could be used to fabricate portable devices for on-site non-destructive assessment of food spoilage indicators.
ANALYTICA CHIMICA ACTA
(2024)
Article
Chemistry, Analytical
Jinting Meng, Zihao Xu, Shasha Zheng, Hongqun Yang, Tianfu Wang, Hong Wang, Yingwei Zhang
Summary: This study demonstrates the development of a cascade signal amplification system using a multi-pedal DNA walker and strand displacement reactions for the electrochemical detection of miRNA-155. The biosensor exhibited high sensitivity, selectivity, and the potential for clinical applications.
ANALYTICA CHIMICA ACTA
(2024)