Per(3-chloro-4-methyl)phenylcarbamate cyclodextrin clicked stationary phase for chiral separation in multiple modes high-performance liquid chromatography

In this work, a detail study has been performed on the enantioselectivity of per(3-chloro-4-methyl) phenylcarbamate-beta-CD clicked chiral stationary phase (CSP) in high-performance liquid chromatography. Both normal phase and polar organic mobile phases have been explored for the enantioseparation of 39 model racemic pairs including aromatic alcohols, flavonoids, beta-blockers and amino acids. Chiral resolution values over 10 were achieved for flavonoids. The comparison study with reference perphenyl-carbamate-beta-CD clicked CSP reveals the significance of 3-chloro-4-methyl functionality in improving the enantioselectivities. This study may provide insight on the multiple interactions between functionalized CD and analytes. (C) 2016 Elsevier B.V. All rights reserved.