Article
Multidisciplinary Sciences
Sruthi Sreekumar, Jasper Wattjes, Anna Niehues, Tamara Mengoni, Ana C. Mendes, Edwin R. Morris, Francisco M. Goycoolea, Bruno M. Moerschbacher
Summary: Chitosans are versatile biopolymers with multiple biological activities and potential applications. Enzymes can be used to partially N-acetylate fully deacetylated chitosan in the presence of excess acetate, replicating the natural control over acetylation pattern and allowing more control over properties.
NATURE COMMUNICATIONS
(2022)
Article
Agriculture, Multidisciplinary
Wenhua Yang, Jianli Zhou, Qiuya Gu, Jean Damascene Harindintwali, Xiaobin Yu, Xiaobo Liu
Summary: Three ginsenoside hydrolases, BG07 (glucoamylase), BG19 (g-glucosidase), and BG23 (g-glucosidase), were identified from Aspergillus tubingensis JE0609 by transcriptome analysis and peptide mass fingerprinting. Among them, BG23 showed high enzymatic activity when expressed in Komagataella phaffii. The enzymatic property studies revealed that BG23 is an acidic and mesophilic enzyme. Furthermore, a one-pot combinatorial enzyme-catalyzed strategy based on BG23 and BGA35 (g-galactosidase from Aspergillus oryzae) achieved a high yield of CK.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Chemistry, Applied
Mounashree J. Urs, Bruno M. Moerschbacher, Stefan Cord-Landwehr
Summary: This method quantifies the amount of N-acetyl-D-glucosamine (GlcNAc) and D-glucosamine (GlcN) units in chitosan polymers by complete enzyme hydrolysis followed by mass spectrometric analysis of the monomers. The method allows calculation of the average fraction of acetylation (x? FA) of the chitinous polymers and is suitable for purified cell walls and whole mycelia.
CARBOHYDRATE POLYMERS
(2023)
Article
Engineering, Environmental
Xuran Liu, Mingting Du, Qi Lu, Dandan He, Kang Song, Qi Yang, Abing Duan, Dongbo Wang
Summary: The study found that chitosan in sewage sludge anaerobic digestion could inhibit methane production by restricting the transfer of organic substrates and reducing microbial activities. Chitosan also suppressed the secretion of extracellular polymeric substances and increased mass transfer resistance, leading to a lower metabolic rate of anaerobes.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2021)
Article
Engineering, Chemical
Dahee Kim, Ju-Hyun Yu, Kyung-Sik Hong, Chan-Duck Jung, Hoyong Kim, Jungbae Kim, Suwan Myung
Summary: This study presents an eco-friendly biomass refinery process for the production and purification of low-molecular-weight xylooligosaccharides (XOS) from agricultural residues. The process involves continuous hydrothermal treatment, membrane-based concentration, xylanase-catalyzed hydrolysis, and enzyme-mediated radical polymerization. The results show high extraction yields of xylan and efficient removal of phenolic compounds, while minimizing XOS loss and waste generation.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Review
Biochemical Research Methods
Trine Gronhaug Halvorsen, Leon Reubsaet
Summary: Selective and efficient sample clean-up is important in mass spectrometric protein and proteomics analyses. This review discusses the utility of molecularly imprinted polymers (MIPs) for protein capture from biological matrices prior to mass spectrometry (MS) analysis. The review covers the application of MIPs in depletion of abundant proteins, protein and proteotypic peptide capture, and capture of post-translational modifications (PTMs), as well as provides an overview of available MIP formats and mass spectrometric techniques used in protein analysis after MIP capture. Overall, the review highlights that MIPs for sample clean-up in mass spectrometric protein and proteomics analysis from biological matrices is still not fully matured.
Article
Green & Sustainable Science & Technology
Zhichao Deng, Qiang Liao, Ao Xia, Yun Huang, Xianqing Zhu, Sheng Qiu, Xun Zhu
Summary: Wheat straw can be converted into biofuel using enzymatic hydrolysis, and a bio-inspired flexible tube reactor was developed to enhance mass transfer and improve the efficiency of the process. The experimental results showed that the reactor had faster diffusion velocity at higher model solution concentrations, and it demonstrated excellent performance in the enzymatic hydrolysis of lignocellulosic slurry.
Article
Medicine, General & Internal
Feras Alzaben, Shawkat Fat'hi, Ayman Elbehiry, Maha Alsugair, Eman Marzouk, Adil Abalkhail, Abdulaziz M. M. Almuzaini, Mohammed Rawway, Mai Ibrahem, Wael Sindi, Turki Alshehri, Mohamed Hamada
Summary: The present study assessed the microbiological quality of ground meats and found that imported meat had higher bacterial contamination compared to local meat. Various bacteria strains and antibiotic resistance were detected, highlighting the importance of improved pathogen treatment and prevention methods. Peptide mass fingerprinting (PMF) was recognized as a powerful analytical method for identifying foodborne pathogens.
Article
Engineering, Chemical
angel Dario Gonzalez-Delgado, Grisel Cogollo-Carcamo, Forlin Bertel-Perez
Summary: This study aimed to carry out a computer-aided environmental evaluation of the industrial-scale production of bioadsorbents based on biopolymers modified with magnetic nanoparticles. The bioadsorbents were found to be non-toxic, effective, and easy to recover. The environmental assessment revealed a potential environmental impact value of -0.78 PEI/kg, with the photochemical oxidation potential category having the greatest impact. The mass integration of the process resulted in a 51% reduction in freshwater consumption and a slight decrease in overall potential impacts.
Article
Energy & Fuels
Kongkona Saikia, Abiram Karanam Rathankumar, Betsy Ann Varghese, Shravani Kalita, Sivanesan Subramanian, Swarnalatha Somasundaram, Vaidyanathan Vinoth Kumar
Summary: An integrated approach was defined for high yield of 5-hydroxymethylfurfural (HMF) from inulin through immobilized enzymes and catalysts, achieving a yield of 96.58%. Both the immobilized enzyme and catalyst showed high operational stability, with the enzyme recycled up to 10 cycles releasing 9.2 g/L of fructose at the 10th cycle and the catalyst recycled up to 10 cycles producing 5.4 g/L of HMF at the 10th cycle.
BIOMASS CONVERSION AND BIOREFINERY
(2021)
Article
Nanoscience & Nanotechnology
Sohyeon Park, Sungwon Jung, Jiwoong Heo, Won-Gun Koh, Sangmin Lee, Jinkee Hong
Summary: This study developed a polysaccharide platform to efficiently deliver C-phycocyanin (C-PC) to myoblast, reducing the cost of cell medium and improving myoblast proliferation in a serum-reduced environment. This innovative technology may have value in meat production.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Engineering, Chemical
Thanida Chuacharoen, Soysruang Aroonsong, Teerin Chysirichote
Summary: This study aimed to optimize the conditions for producing alginate using sugar cane juice as the main carbon source. The results showed that both pH value and nitrogen concentration affected alginate production, and the pH value of the culture affected the molecular weight of alginate obtained. A regression model was developed to predict alginate production.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2022)
Article
Green & Sustainable Science & Technology
Wen Wang, Mengxuan Zhang, Shijun Liu, Qiong Wang, Yunzi Hu, Cuiyi Liang, YunYun Liu, Hui Liu, Wei Qi
Summary: High energy consumption is a major obstacle for the conversion of lignocellulose into biofuels and biochemicals. In this study, a self-designed pilot-scale device with recycling of black liquor and waste washing water was used for low-temperature pretreatment and high-solid enzymatic hydrolysis of sugarcane bagasse. The results showed that the enzymatic hydrolysis of the treated sugarcane bagasse achieved a high glucose concentration and glucan conversion. A low-energy strategy for cellulosic ethanol production with low emission was proposed.
JOURNAL OF CLEANER PRODUCTION
(2022)
Review
Agriculture, Multidisciplinary
Sara Casalini, Marco Giacinti Baschetti
Summary: In recent years, there has been growing interest in sustainable food packaging systems with additional functionality. This review focuses on the state of the art of active renewable packaging, particularly antimicrobial systems containing nanocellulose and chitosan. The characteristics of nanocellulose, chitosan, and different essential oils used in active packaging are introduced, and the latest research on nanocellulose- and chitosan-based active packaging is presented. Essential oils have proven to be effective in antimicrobial properties and positive effects on material properties. However, due to the wide variety of materials, it is challenging to establish general guidelines for the development of active packaging systems.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2023)
Article
Engineering, Environmental
Patrick L. Tomco, Khrystyne N. Duddleston, Adrienne Driskill, Jasmine J. Hatton, Kirsten Grond, Toshia Wrenn, Matthew A. Tarr, David C. Podgorski, Phoebe Zito
Summary: Chemical herders and in-situ burning (ISB) are methods used to mitigate oil spills in the high latitude marine environment. This experiment found that ISB and ISB+OP40 samples had higher aromatic classes and differences in microbial diversity compared to seawater. The findings provide valuable insights for understanding the impact of chemical herders and ISB in high latitude marine environments.
JOURNAL OF HAZARDOUS MATERIALS
(2022)
Article
Chemistry, Physical
Dounia Sid, Milad Baitiche, Lekhmici Arrar, Ferhat Djerboua, Riadh Bourzami, Pierre Alcouffe, Mokhtar Boutahala, Antonio Gil, Laurent David, Marc Le Borgne
Summary: In this study, halloysite was elegantly modified with citric acid and used as a drug carrier for ketoprofen. The inclusion complexes of Hal-CA with KET showed increased anti-inflammatory and antinociceptive potentials, as well as maximum protection against ulcers. These findings suggest that Hal-CA could be a promising carrier for pharmaceutical formulations.
APPLIED CLAY SCIENCE
(2022)
Article
Materials Science, Composites
Clementine Beutier, Laurent David, Guillaume Sudre, Philippe Cassagnau, Patrick Heuillet, Bernard Cantaloube, Anatoli Serghei
Summary: The study demonstrates a relationship linking the evolution of electrical conductivity during mechanical deformation to the mechanical Mullins effect, revealing a characteristic electrical signature. Significant differences between the electrical and mechanical Mullins effects are found, particularly in the kinetics of property stabilization after the first mechanical deformation cycle.
COMPOSITES SCIENCE AND TECHNOLOGY
(2022)
Article
Engineering, Biomedical
Ysander von Boxberg, Sylvia Soares, Camille Giraudon, Laurent David, Maud Viallon, Alexandra Montembault, Fatiha Nothias
Summary: Direct contact with chitosan-FPHS can modify the polarization of bone marrow-derived macrophages, favoring the generation of anti-inflammatory subtypes. Lower degree of acetylation and higher chitosan concentration promote macrophage polarization towards anti-inflammatory subtypes.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2022)
Article
Biochemistry & Molecular Biology
Philipp Lemke, Lena Juenemann, Bruno M. Moerschbacher
Summary: Several recent studies have shown that intensive agriculture plays a significant role in global climate change and biodiversity decline. Synthetic pesticides and fertilizers, which are necessary for high crop yields to meet the demands of a growing world population, are major contributors to these negative effects. However, the combination of biopesticides and biostimulants with organic farming methods can help reduce the use of agro-chemicals. This study demonstrates that the partial hydrolysis of chitosan can enhance its antifungal properties and allow for a synergistic effect when combined with copper acetate, leading to a significant reduction in copper concentration while maintaining the effectiveness of the treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Flor Estefany Bentley, Renaud Passieux, Laurent David, Anayancy Osorio-Madrazo
Summary: This study investigated the effects of low molar mass chitosan on chitosan fibers with bimodal distributions of molar masses. The results showed that adding low molar mass chitosan slightly increased the crystallinity of chitosan films, but decreased the crystallinity and altered the mechanical properties of stretched fibers.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Correction
Multidisciplinary Sciences
Lea Hembach, Stefan Cord-Landwehr, Bruno M. Moerschbacher
SCIENTIFIC REPORTS
(2022)
Article
Materials Science, Biomaterials
Renaud Passieux, Guillaume Sudre, Alexandra Montembault, Martine Renard, Agnes Hagege, Pierre Alcouffe, Ali Haddane, Marie Vandesteene, Nadege Boucard, Laurence Bordenave, Laurent David
Summary: This study investigates the wet spinning of cytocompatible, bioresorbable, and knittable chitosan monofilaments with incorporated Cu2+ and Zn2+ ions. The results show that a concentration of Cu2+ at 0.01 and Zn2+ at 0.03 significantly enhances the antibacterial activity without compromising cytocompatibility. Additionally, the wet-spun CTS-Cu monofilaments are found to be knittable under industrial conditions.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2022)
Article
Pharmacology & Pharmacy
Paul Rocchi, Delphine Brichart-Vernos, Francois Lux, Isabelle Morfin, Laurent David, Claire Rodriguez-Lafrasse, Olivier Tillement
Summary: This study developed a multimodal ultrasmall nanoparticle, CuPRiX, for cancer treatment. CuPRiX nanoparticles can prevent tumor cell dissemination and metastasis by depleting copper in tumors, and they also increase the sensitivity of tumors to radiotherapy.
Article
Biotechnology & Applied Microbiology
Anna Niehues, Daniele Bizzarri, Marcel J. T. Reinders, P. Eline Slagboom, Alain J. van Gool, Erik B. van den Akker, Peter A. C. 't Hoen
Summary: Population-scale expression profiling studies provide valuable insights into biological and disease mechanisms. Missing or incomplete phenotypic information can hinder data analysis. Metabolomics-based surrogates can be used as predictors for clinical phenotypes. In this study, metabolomic surrogates were evaluated for 17 traits using expression profiling data. The results showed that metabolomic surrogates performed similarly to reported phenotypes.
Article
Polymer Science
Clementine Beutier, Anatoli Serghei, Philippe Cassagnau, Patrick Heuillet, Bernard Cantaloube, Nathan Selles, Isabelle Morfin, Guillaume Sudre, Laurent David
Summary: In this study, in situ coupled mechanical/electrical/WAXS/SAXS investigations were carried out on EPDM-based composite materials filled with carbon black (CB) under uniaxial tensile stretching. The study reveals the correlation between the electrical conductivity and the microstructural state of the composite, providing insights into the mechanisms governing the structure/properties relationships with increasing deformation. The formation of nanovoids at large deformations leads to a significant decrease in conductivity. This study contributes to a better understanding of the conductivity/strain relationships of carbon black filled elastomers and has potential applications in developing smart rubber materials.
Article
Electrochemistry
Sven Kuenne, Frederik Puettmann, Max Linhorst, Bruno M. Moerschbacher, Martin Winter, Jie Li, Tobias Placke
Summary: This study investigates chitosan-based biopolymers as green binders for positive electrodes, with low DP chitosan showing better adhesion strength and potential for reducing binder content.
Article
Chemistry, Physical
Arnaud Kamdem Tamo, Tuan Anh Tran, Ingo Doench, Shaghayegh Jahangir, Aastha Lall, Laurent David, Carlos Peniche-Covas, Andreas Walther, Anayancy Osorio-Madrazo
Summary: This study proposes a 3D printing method for a multifunctional hydrogel biomaterial with bioactivity, good mechanical properties, and biodegradability. The method utilizes encapsulated cellulase and cellulose nanofiber filled chitosan viscous suspensions as bioinks to 3D print enzymatic biodegradable and biocompatible cellulose nanofiber reinforced chitosan hydrogels. The incorporation of the enzyme within the hydrogel helps control the degradation of cellulose while maintaining the cell growth-promoting property of chitosan. The 3D printed composite hydrogels exhibit excellent cytocompatibility and biodegradability.
Review
Nanoscience & Nanotechnology
Christy Kwokdinata, Vaibavi Ramanujam, Jiahui Chen, Paula Nunes de Oliveira, Mui Hoon Nai, Wai Hon Chooi, Chwee Teck Lim, Shi Yan Ng, Laurent David, Sing Yian Chew
Summary: This study found that using injectable hyaluronan-gelatin hydrogel in transplantation therapy can promote cell survival and neural differentiation of human spinal cord progenitor cells.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Medicine, General & Internal
Elsa Labrune, Cyrielle Fournier, Benjamin Riche, Laurent David, Alexandra Montembault, Sophie Collardeau-Frachon, Mehdi Benchaib, Jacqueline Lornage, Jean Iwaz, Bruno Salle
Summary: This study tested the long-term development ability of human ovarian cortex cells in unconventional culture conditions and found that bioreactor cultures slightly outperformed traditional cultures in folliculogenesis. This has important implications for follicle preservation and long-term toxicological studies.
MEDICINA-LITHUANIA
(2022)
