期刊
JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY
卷 28, 期 4, 页码 405-413出版社
SPRINGER INDIA
DOI: 10.1007/s13562-019-00500-8
关键词
Cold shock protein; Pseudomonas koreensis; Psychrophiles; Gene expression; Molecular modeling
资金
- network project 'Application of Microorganisms in Agriculture and Allied Sectors (AMAAS)' from Indian Council of Agricultural Research (ICAR), India
- CRP Genomics from Indian Council of Agricultural Research (ICAR), India
Cold shock proteins (CSPs) are greatly conserved family of structurally related DNA binding proteins which are produced during temperature drift. A 213 bp long cspA gene was cloned and sequenced from Pseudomonas koreensis P2 in the present study. The expression analysis of the cspA showed > 2.5 folds increase in the mRNA level at 15 degrees C while the expression was almost on par at 30 degrees C and 5 degrees C indicating its role in moderately low temperature. In silico analyses of the gene showed that the gene codes for 7.69 kDa protein which was phylogenetically very similar to CspA present in Pseudomonads. Amino acid composition of the CspA from P. koreensis was different from that of mesophilic Pseudomonas and tiny/small amino varied significantly between CspA of cold adaptive and mesophilic species. The CspA from P. koreensis P2 contained RNP motifs involved in binding of DNA and RNA. Phylogenetic analyses revealed that the CspA protein of P. koreensis P2 was more close to CspA of distant subgroups of Pseudomonas like P. fluorescens and P. putida subgroup indicating a possible intra-specific gene transfer.
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