期刊
JOURNAL OF NEUROSCIENCE
卷 39, 期 20, 页码 3970-3982出版社
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.2865-18.2019
关键词
astrocytes; EAAT2; Glu imaging; glutamate clearance; synapse pathology; tripartite synapse
资金
- CHDI [A-12467]
- German Research Foundation [Exc 257/1]
- intramural Charite Research Funds
- Biotechnology and Biological Sciences Research Council [BB/M02556X/1, BB/S003894/1]
- British Heart Foundation Intermediate Basic Science Research fellowship [FS/17/56/32925]
- BBSRC [BB/M02556X/1, BB/S003894/1] Funding Source: UKRI
Changes in the balance between glutamate (Glu) release and up take may stimulate synaptic reorganization and even synapse loss. In the case of neurodegeneration, a mismatch between astroglial Glu uptake and presynaptic Glu release could be detected if both parameters were assessed independently and at a single-synapse level. This has now become possible due to a new imaging assay with the genetically encoded ultrafast Glu sensor iGlu(u). We report findings from individual corticostriatal synapses in acute slices prepared from mice of either sex that were > 1 year of age. Contrasting patterns of short-term plasticityanda size criterion identified two classes of terminals, presumably corresponding to the previously defined IT (intratelencephalic) and PT (pyramidal tract) synapses. The latter exhibited a higher degree of frequency potentiation/residual Glu accumulation and were selected for our first iGlu(u) single-synapse study in Q175 mice, a model of Huntington's disease (HD). In HD mice, the decay time constant of the perisynaptic Glu concentration (TauD), as an indicator of uptake, and the peak iGlu(u) amplitude, as an indicator of release, were prolonged and reduced, respectively. Treatment of WT preparations with the astrocytic Glu uptake blocker TFB-TBOA (100 nM) mimicked the TauD changes in homozygotes. Considering the largest TauD values encountered in WT, similar to 40% of PT synapses tested in Q175 heterozygotes can be classified as dysfunctional. Moreover, HD but not WT synapses exhibited a positive correlation between TauD and the peak amplitude of iGlu(u). Finally, EAAT2 (excitatory amino acid transport protein 2) immunoreactivity was reduced next to corticostriatal terminals. Thus, astrocytic Glu transport remains a promising target for therapeutic intervention.
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