Article
Agriculture, Multidisciplinary
Chao Tang, Hongyuan Zhao, Liangyu Kong, Fanqiang Meng, Libang Zhou, Zhaoxin Lu, Yingjian Lu
Summary: This study investigated the effects of Lactobacillus acidophilus NX2-6-fermented yogurt (LA-Y) on lipid and glucose levels in high-fat diet-fed mice. The results showed that LA-Y improved lipid profile, reduced fasting blood glucose levels, and enhanced insulin sensitivity. LA-Y also alleviated glucose metabolism disorders by activating the insulin signaling pathway, reducing inflammation, and promoting glycolysis and gluconeogenesis in the small intestine. Additionally, LA-Y promoted fat browning and increased energy expenditure through adiponectin/AMPK alpha/PGC-1 alpha/UCP1 pathway and mitochondrial biogenesis via adiponectin/AdipoR1/APPL1/AMPK alpha/PGC-1 alpha pathway.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Jiakun Dai, Hongli Shi, Chao Zhang, Bo Li, Yan Li, Yahui Wei
Summary: In this study, nanoparticles anchored with adiponectin (AP) were designed and prepared to mimic multimeric AP. These nanoparticles can mimic the natural multimeric AP by anchoring the AP monomer to the surface of PLGA/PHB nanoparticles. Similar to the natural multimeric AP, the multimeric AP mimetic nanoparticles can upregulate NF-kappa B and have no significant effect on AMPK in muscle or endothelial cells. Furthermore, multimeric AP nanoparticles can activate PI3K, AKT, and the multimeric AP receptor T-cadherin, reducing blood glucose and serum glycoprotein levels in mice.
PROCESS BIOCHEMISTRY
(2023)
Article
Agriculture, Dairy & Animal Science
Jiangxue Cai, Qingmei Hu, Hai Lin, Jingpeng Zhao, Hongchao Jiao, Xiaojuan Wang
Summary: This study found that the expression of adiponectin varies in different tissues in broilers, and feed restriction can promote the utilization of fatty acids to maintain energy balance in the body.
Article
Endocrinology & Metabolism
Keitaro Kawada, Shunbun Kita, Shiro Fukuda, Keita Fukuoka, Tomonori Okita, Emi Kawada-Horitani, Masahito Iioka, Taka-aki Sakaue, Yusuke Kawachi, Kohei Fujii, Yu Kimura, Shuichi Otabe, Yuya Fujishima, Hitoshi Nishizawa, Norikazu Maeda, Iichiro Shimomura
Summary: In this study, overexpression of human adiponectin in mice increased the number of circulating exosomes, but did not significantly improve glucose metabolism, change body weights, or alter triglyceride clearance under a high-fat diet. Therefore, human adiponectin overexpression in mice increased plasma exosomes but did not significantly influence glucose and lipid metabolism.
Article
Endocrinology & Metabolism
Keitaro Kawada, Shunbun Kita, Shiro Fukuda, Keita Fukuoka, Tomonori Okita, Emi Kawada-Horitani, Masahito Iioka, Taka-aki Sakaue, Yusuke Kawachi, Kohei Fujii, Yu Kimura, Shuichi Otabe, Yuya Fujishima, Hitoshi Nishizawa, Norikazu Maeda, Iichiro Shimomura
Summary: The study demonstrates that adiponectin increases plasma exosomes without significantly influencing glucose and lipid metabolism. This suggests that the promotion of exosome production by adiponectin may not have a significant impact on metabolic processes.
Article
Cell Biology
Abigail E. Cullen, Ann M. Centner, Riley Deitado, Ahmed Ismaeel, Panagiotis Koutakis, Judy Muller-Delp, Gloria Salazar
Summary: Adiponectin plays a beneficial role in the cardiovascular system by preserving the contractile phenotype of vascular smooth muscle cells. However, the lack of adiponectin leads to abnormalities in AKT signaling, the extracellular matrix, and TGF-beta signaling. Additionally, adiponectin deficiency affects oxidative phosphorylation, glycolysis, and mitochondrial reactive oxygen species levels. Anti-atherogenic mechanisms involve regulating MMPs and decorin in the extracellular matrix.
Review
Medicine, Research & Experimental
Zhaodan Wang, Zehao Chen, Fuchun Fang, Wei Qiu
Summary: Adiponectin, secreted primarily by adipose tissue, plays anti-inflammatory and metabolic regulatory roles. There is an association between adiponectin and periodontitis, a localized inflammatory disease that may lead to the destruction of periodontal supporting apparatus and subsequent tooth loss. Adiponectin and its agonists show promise in the treatment of periodontitis, with further exploration needed on underlying mechanisms and clinical applications.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Physiology
Susanne Rohrbach, Ling Li, Tatyana Novoyatleva, Bernd Niemann, Fabienne Knapp, Nicole Molenda, Rainer Schulz
Summary: PCSK9 affects LRP1-mediated signaling pathways in cardiomyocytes, influencing the metabolic effects of CTRP9, which is the CTRP with the highest homology to adiponectin. Knockdown of LRP1 reduces the cellular effects induced by CTRP9.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Mineyuki Mizuguchi, Takeshi Yokoyama, Takahito Otani, Shiori Kuribara, Yuko Nabeshima, Takayuki Obita, Masato Hirata, Keiichi Kawano
Summary: An acidic environment in bone is crucial for bone metabolism and the production of decarboxylated osteocalcin. The crystal structure of decarboxylated osteocalcin at pH 2.0 showed that it retains the alpha-helix structure and is stable under acidic conditions. Additionally, the activity of decarboxylated osteocalcin in inducing adiponectin is related to specific mutations at Glu17 and Glu21.
Article
Pharmacology & Pharmacy
Kehinde S. Olaniyi, Adesola A. Oniyide, Oluwaseun A. Adeyanju, Lekan S. Ojulari, Adams O. Omoaghe, Oluranti E. Olaiya
Summary: The study found that low-dose spironolactone (LDS) can improve endocrine-metabolic disturbances and inflammation-related comorbidities in experimentally-induced PCOS rats by modulating the circulating androgen-adiponectin status.
TOXICOLOGY AND APPLIED PHARMACOLOGY
(2021)
Article
Cell Biology
Yachun Han, Shan Xiong, Hao Zhao, Shikun Yang, Ming Yang, Xuejing Zhu, Na Jiang, Xiaofen Xiong, Peng Gao, Ling Wei, Ying Xiao, Lin Sun
Summary: The deficiency of autophagy-mediated lipophagy plays a critical role in the ectopic lipid deposition and renal injury related to lipids in diabetic nephropathy (DN). Decreased lipophagy, increased ectopic lipid deposition and lipotoxicity were observed in tubular cells of DN patients and db/db mice, and these effects could be reversed by the autophagy-promoting adiponectin receptor activator AdipoRon.
CELL DEATH & DISEASE
(2021)
Article
Medicine, General & Internal
Beata Czerwienska, Michal Lelek, Damian Gojowy, Stanislaw Surma, Katarzyna Mizia-Stec, Andrzej Wiecek, Marcin Adamczak
Summary: This study aimed to assess the effect of renal denervation on plasma adiponectin concentration in patients with resistant arterial hypertension. The results showed that plasma adiponectin concentration significantly increased after successful renal denervation. Clinical consequences of these results need further investigations, but it suggests that successful renal denervation in hypertensive patients may provide cardiovascular benefits beyond blood pressure reduction by increasing plasma adiponectin concentration.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Cell Biology
Karel M. Lopez-Vilaret, Jose L. Cantero, Marina Fernandez-Alvarez, Miguel Calero, Olga Calero, Monica Lindin, Montserrat Zurron, Fernando Diaz, Mercedes Atienza
Summary: The study suggests that in cognitively normal older adults, insulin resistance, circulating triglyceride, and glucose levels have an impact on the neuroprotective effect of adipocytokines, indicating that the neuroprotective role of adipocytokines is influenced by multiple factors.
Article
Endocrinology & Metabolism
Pedro Cisternas, Camila Gherardelli, Joel Gutierrez, Paulina Salazar, Carolina Mendez-Orellana, G. William Wong, Nibaldo C. Inestrosa
Summary: The link between metabolic syndrome and brain function is not well understood, but adipokines, including adiponectin and resistin, may play a role. A high-fat diet-induced model revealed that adiponectin treatment improved cognitive functions and reduced AD pathology, while resistin exacerbated pathology.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Nuri Song, Da Yeon Jeong, Thai Hien Tu, Byong Seo Park, Hye Rim Yang, Ye Jin Kim, Jae Kwang Kim, Joon Tae Park, Jung-Yong Yeh, Sunggu Yang, Jae Geun Kim
Summary: The study found that adiponectin enhances glucose uptake, glycolytic processes, and fatty acid oxidation in primary hypothalamic astrocytes, as well as promotes the synthesis and release of monocarboxylates. These results suggest that adiponectin triggers catabolic processes in astrocytes, therefore enhancing nutrient availability in the hypothalamus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Michele Mannelli, Tania Gamberi, Francesca Magherini, Tania Fiaschi
Summary: This study demonstrates that cachexia induction in myotubes is associated with a metabolic shift towards fermentation, and inhibiting lactate formation can prevent cachexia. The findings suggest lactate dehydrogenase as a possible new tool for counteracting the onset of this pathology.
Article
Cell Biology
Caterina Bernacchioni, Roberta Squecco, Tania Gamberi, Veronica Ghini, Fabian Schumacher, Michele Mannelli, Rachele Garella, Eglantina Idrizaj, Francesca Cencetti, Elisa Puliti, Paola Bruni, Paola Turano, Tania Fiaschi, Chiara Donati
Summary: This study provides experimental evidence that S1P signaling is modulated by globular Adn treatment, increasing the phosphorylation of SK1/2 and mRNA expression levels of S1P(4) in C2C12 myotubes. The involvement of the S1P axis in Adn action was highlighted, as inhibition of SK1 and 2 significantly inhibited the electrophysiological changes, oxygen consumption, and amino acid levels induced by the hormone. These findings demonstrate the importance of S1P biosynthesis for the electrophysiological properties and oxidative metabolism of Adn in skeletal muscle cells.
Article
Geriatrics & Gerontology
Lisa Gambarotto, Samuele Metti, Martina Chrisam, Cristina Cerqua, Patrizia Sabatelli, Andrea Armani, Carlo Zanon, Marianna Spizzotin, Silvia Castagnaro, Flavie Strappazzon, Paolo Grumati, Matilde Cescon, Paola Braghetta, Eva Trevisson, Francesco Cecconi, Paolo Bonaldo
Summary: The study reveals that Ambra1 plays a critical role in the mitophagic flux of adult murine skeletal muscle and its deficiency leads to abnormal mitochondria and myofiber remodeling.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2022)
Article
Cell Biology
Ilaria Gregorio, Maddalena Mereu, Gabriella Contarini, Luca Bello, Claudio Semplicini, Francesca Burgio, Loris Russo, Stefania Sut, Stefano Dall'Acqua, Paola Braghetta, Carlo Semenza, Elena Pegoraro, Francesco Papaleo, Paolo Bonaldo, Matilde Cescon
Summary: Study finds that COL6 deficiency affects sensorimotor gating, memory/attention functions, and dopaminergic signaling. In vitro experiments confirm the direct link between COL6 ablation and defective dopaminergic activity. Patients with COL6-related myopathies also show deficits in attentional control abilities. These findings highlight the importance of COL6 in maintaining dopamine circuitry function and related neurobehavioral features.
DISEASE MODELS & MECHANISMS
(2022)
Article
Biochemistry & Molecular Biology
Michele Mannelli, Tania Gamberi, Rachele Garella, Francesca Magherini, Roberta Squecco, Tania Fiaschi
Summary: Cachexia, a systemic disease associated with several pathologies, can be prevented in myotubes by the addition of sodium pyruvate in conditioned media from CT26 colon cancer cells. Sodium pyruvate inhibits the metabolic modifications responsible for the acquisition of cachectic features, and also decreases phosphorylation level of STAT3.
Article
Biochemistry & Molecular Biology
Valentina Tonelotto, Chiara Consorti, Nicola Facchinello, Valeria Trapani, Patrizia Sabatelli, Chiara Giraudo, Marianna Spizzotin, Matilde Cescon, Cristiano Bertolucci, Paolo Bonaldo
Summary: By using CRISPR/Cas9 technology, a zebrafish COL6 knockout line was generated, which displayed neuromuscular defects and motor dysfunctions similar to patients with COL6-related myopathies. The knockout fish also exhibited alterations in craniofacial cartilage structure. Inhibition of BMP activity and treatment with a beta(2)-adrenergic receptor agonist showed improvements in locomotor activity and motility defects in the knockout fish.
Article
Biochemistry & Molecular Biology
Damiano Cirri, Andrea Geri, Lara Massai, Michele Mannelli, Tania Gamberi, Francesca Magherini, Matteo Becatti, Chiara Gabbiani, Alessandro Pratesi, Luigi Messori
Summary: A panel of four novel gold(I) complexes, inspired by the clinically established gold drug auranofin, were prepared and characterized. The differences in their interactions with model proteins were analyzed, and their cytotoxic effects on ovarian cancer cells were quantitated. These novel gold complexes showed potential as effective anticancer agents.
Article
Clinical Neurology
Mariarita Galbiati, Marco Meroni, Marina Boido, Matilde Cescon, Paola Rusmini, Valeria Crippa, Riccardo Cristofani, Margherita Piccolella, Veronica Ferrari, Barbara Tedesco, Elena Casarotto, Marta Chierichetti, Marta Cozzi, Francesco Mina, Maria Elena Cicardi, Silvia Pedretti, Nico Mitro, Anna Caretto, Patrizia Rise, Angelo Sala, Andrew P. Lieberman, Paolo Bonaldo, Maria Pennuto, Alessandro Vercelli, Angelo Poletti
Summary: Spinal and bulbar muscular atrophy (SBMA) is a neuromuscular disease characterized by motor neuron degeneration, muscle weakness, and an expansion of the CAG triplet repeat in the androgen receptor (AR) gene. In this study, the use of the antiandrogen bicalutamide and the natural disaccharide trehalose showed promising results in ameliorating SBMA pathology in a mouse model. These compounds reduced ARpolyQ toxicity, prevented autophagic flux blockage, and improved muscle morphology and function, suggesting their potential for future clinical trials in SBMA patients.
Article
Nanoscience & Nanotechnology
Marta Rojas-Rodriguez, Tania Fiaschi, Michele Mannelli, Leonardo Mortati, Federica Celegato, Diederik S. Wiersma, Camilla Parmeggiani, Daniele Martella
Summary: Liquid Crystalline Networks (LCNs) with different surface topographies obtained by self-assembly can efficiently control cell alignment and differentiation, providing biological models that cannot be reproduced spontaneously on standard culture dishes. These materials can guide the organization of different cell lines, including single cell alignment or high-density cell cultures. The rough surfaces formed by the spontaneous assembly of liquid crystals can control biological models without the need for lithographic patterning or complex fabrication procedures, opening up possibilities for in vitro formation of well-aligned muscle tissue.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemistry & Molecular Biology
Lorenzo Chiaverini, Emma Baglini, Michele Mannelli, Valeria Poggetti, Federico Da Settimo, Sabrina Taliani, Tania Gamberi, Elisabetta Barresi, Diego La Mendola, Tiziano Marzo
Summary: Auranofin, a gold-based drug, is clinically used to treat arthritis. Recently, it has shown promising effects against various tumors, including ovarian cancer. Evidence suggests that its antiproliferative properties are mainly due to the inhibition of thioredoxin reductase (TrxR) in the mitochondrial system. In this study, a novel complex was synthesized and evaluated for its anticancer effects, combining a phenylindolylglyoxylamide ligand with the auranofin-derived cationic fragment. This study provides proof-of-concept for targeted therapy by coupling PIGA ligands with anticancer gold active moieties.
Review
Biochemistry & Molecular Biology
Alberto Di Martino, Matilde Cescon, Claudio D'Agostino, Francesco Schilardi, Patrizia Sabatelli, Luciano Merlini, Cesare Faldini
Summary: Collagen VI has diverse functions in tissues, including mechanical roles, cytoprotective functions, and regulation of cell differentiation and autophagy. Mutations in COL6A1, COL6A2, and COL6A3 genes cause muscular disorders such as UCMD, BM, and MM, which present with muscle wasting and weakness, joint contractures, and respiratory compromise. Currently, effective therapeutic strategies are lacking, and the effects of collagen VI mutations on other tissues are poorly understood. This review aims to outline the role of collagen VI in the musculoskeletal system and provide an update on its tissue-specific functions to bridge the knowledge gap between scientists and clinicians managing collagen VI-related myopathies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Sonia Calabro, Svenja Kankowski, Matilde Cescon, Giovanna Gambarotta, Stefania Raimondo, Kirsten Haastert-Talini, Giulia Ronchi
Summary: It is widely recognized that the gut microbiota plays a crucial role in human health, and its disruption is associated with various diseases. Recent studies have expanded our understanding of the relationship between the gut microbiota and the peripheral nervous system (PNS), highlighting that the PNS can be affected by GM perturbations. This review provides a comprehensive summary of the current knowledge on the impact of the gut microbiota on the PNS, considering both its somatic and autonomic divisions in physiological, regenerative, and pathological conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Lisa Gambarotto, Samuele Metti, Matteo Corpetti, Martina Baraldo, Patrizia Sabatelli, Silvia Castagnaro, Matilde Cescon, Bert Blaauw, Paolo Bonaldo
Summary: COL6-related myopathies (COL6-RM) are inherited muscle disorders caused by mutations of COL6 genes, characterized by early-onset muscle weakness. Targeting autophagy through dietary spermidine administration has shown beneficial effects in col6a1(-/-) mice and COL6-RM patients. A 100-day-long spermidine regimen rescues muscle strength in col6a1(-/-) mice and improves mitochondria and neuromuscular junction integrity, suggesting its potential application in clinical trials for COL6-RM.
Article
Biochemistry & Molecular Biology
Matilde Cescon, Elena Rampazzo, Silvia Bresolin, Francesco Da Ros, Lorenzo Manfreda, Alice Cani, Alessandro Della Puppa, Paola Braghetta, Paolo Bonaldo, Luca Persano
Summary: Collagen VI has a distinct distribution within GBM tumors and is strongly correlated with aggressive and phenotypically immature cells. It sustains the stem-like properties of GBM cells and promotes cancer cell proliferation and survival. Collagen VI plays a pivotal role in GBM tumor biology, affecting phenotypic identity, gene transcription, and response to treatment.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Meeting Abstract
Biochemical Research Methods
Paola Braghetta, Nicola Facchinello, Giulia Pigato, Dario Bizzotto, Aurora De Acutis, Matilde Cescon, Paola Brun, Giovanni Vozzi
TRANSGENIC RESEARCH
(2022)
