期刊
AMINO ACIDS
卷 48, 期 6, 页码 1357-1372出版社
SPRINGER WIEN
DOI: 10.1007/s00726-016-2204-5
关键词
Fluorescent labeling; Fluorescent dyes; Site-specific labeling; Bioorthogonal reactions; Chemical tags
This review focuses on the various approaches to covalently attach a chromophore to a biomolecule of interest in site-specific manner. Novel methods like inverse electron-demand Diels-Alder reaction, Pictet-Spengler ligation and enzyme tags like SNAP and Halo-tags are critically discussed and compared to established techniques like copper-free click reaction and native chemical ligation. Selected examples in which the tags have been exploited for in vitro or in vivo imaging are reviewed and evaluated.
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