Review
Biochemical Research Methods
Rui Wang, Hui-Da Li, Ying Cao, Zi-Yi Wang, Ting Yang, Jian-Hua Wang
Summary: Viruses, specifically M13 phage, are being utilized in biosensing and biomedicine due to their versatility, specific reactivities, and responsiveness to genetic modifications. M13 phages can be engineered and modified to create a multifunctional analysis platform, with different functional regions performing their tasks without interference. The unique filamentous morphology and flexibility of M13 phages enhance target affinity and signal amplification in analytical applications.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Biomedical
Cheng Chang, Wennan Guo, Xinbo Yu, Chaoyi Guo, Nan Zhou, Xiaokui Guo, Ru-Lin Huang, Qingtian Li, Yongzhang Zhu
Summary: Bacteriophages, particularly M13 phages, have been extensively studied in the field of nanomedicine due to their unique biological advantages and engineering capabilities. The nanofiber-like morphology of M13 phages allows them to reach various target sites and self-assemble into multidimensional scaffolds. Genetic modifications enable the display of peptides and antibodies on the phages, making them valuable for precise and individualized medicine. Researchers have also used novel engineering approaches to enhance the bionanomaterial properties of M13 phages, leading to applications in drug delivery, biodetection, tissue regeneration, and targeted cancer therapy.
MATERIALS TODAY BIO
(2023)
Article
Food Science & Technology
Yuting Wu, Bing Liu, Zhiwei Liu, Pengjie Zhang, Xihui Mu, Zhaoyang Tong
Summary: In this study, a nonpathogenic model for COVID-19 research was constructed using phage display technology. The model successfully expressed the nucleocapsid protein of SARS-CoV-2 and showed potential as a standard for qPCR quantification and antibody affinity. Additionally, the model was used to develop a system for the classification and identification of samples using ATR-FTIR.
Review
Chemistry, Analytical
Ping Xu, Subhadeep Ghosh, Anam Rana Gul, Jigna R. Bhamore, Jong Pil Park, Tae Jung Park
Summary: Biosensors have seen rapid growth in the field of biological detection and biomedical diagnosis, with displayed peptides from phage display libraries being recognized as a promising alternative to antibodies. The advantages of displayed peptides include high affinity, low cost, ease of design and modification, mass production, and increased resistance to complex environments.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Zhenqiang Shi, Xiancheng Zhang, Xijing Yang, Xiaoyu Zhang, Fei Ma, Hui Gan, Junjun Chen, Dongdong Wang, Wenjing Sun, Jingxia Wang, Cunli Wang, Liting Lyu, Kaiguang Yang, Lijing Deng, Guangyan Qing
Summary: A robust strategy based on phage display screening and hemocompatible peptide bottlebrush polymer design is proposed for specific clearance of targeted Lipopolysaccharide (LPS) from circulating blood. A novel peptide with high affinity, specificity, and neutralization activity against the targeted LPS is discovered. This work provides a universal paradigm for developing a highly selective hemoadsorbent library fully covering the LPS family, promising for a new era of precision medicine in sepsis therapy.
ADVANCED MATERIALS
(2023)
Article
Biochemical Research Methods
Han Ying Wang, You-Chiun Chang, Che-Wei Hu, Chia-Yi Kao, Yao-An Yu, See-Khai Lim, Kurt Yun Mou
Summary: A novel therapeutic platform utilizing filamentous phage to display an antitumor cytokine was developed and demonstrated significant antitumor activities in a murine colorectal cancer model. The combined therapy of the cytokine phage and radiation greatly improved therapeutic potency, leading to 100% survival rate and 25% complete remission rate, indicating the potential for multifunctional nanoparticles for cancer therapy.
ACS SYNTHETIC BIOLOGY
(2021)
Article
Biophysics
Liyan Bi, Huangruici Zhang, Wenchao Hu, Jiadong Chen, Yixuan Wu, Hao Chen, Bingqian Li, Zhiyang Zhang, Jaebum Choo, Lingxin Chen
Summary: In this study, a novel M13 phage-based SERS nanocarrier was developed for the detection and inactivation of E. coli. The M13 phage was used as a biological scaffold to conjugate with Au@Ag core-shell nanorods (Au@AgNR) and specifically target E. coli. The proposed SERS probe showed a detection limit of 0.5 cfu/mL and high antibacterial efficiency of approximately 90% against E. coli.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Multidisciplinary
Shuxu Wang, Noriyuki Uchida, Kento Ueno, Teruhiko Matsubara, Toshinori Sato, Takuzo Aida, Yasuhiro Ishida
Summary: This study reports that the magnetic orientation of M13 filaments vertical to the target surface significantly affects the phage display selection. The method of using magnetic orientation during library selection can control the interference by the M13 main body and alter the population of clones obtained.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Applied
Jae Hwan Shin, Tae Jung Park, Moon Seop Hyun, Jong Pil Park
Summary: This study aimed to create highly sensitive and selective phage-based electrochemical biosensors for detecting ovomucoid, a known food allergen. By performing phage display using two different libraries, two phage clones displaying affinity peptides for ovomucoid were selected. The developed phage sensor, covalently attached to a gold electrode, exhibited significantly better binding affinity and limit of detection compared to the linear phage sensor, showing good reproducibility and recovery even in actual samples.
Article
Immunology
Zohreh Safari, Majid Sadeghizadeh, Golareh Asgaritarghi, Hassan Bardania, Dina Sadeghizadeh, Sara Soudi
Summary: This study found that M13 phages can polarize macrophages towards the M2 phenotype by inducing anti-inflammatory cytokine production and reducing inflammatory cytokine levels. This suggests the potential of M13 phages in immunotherapy.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2022)
Article
Chemistry, Analytical
Nuriye Korkmaz, Changhyun Hwang, Kim Kristin Kessler, Yuliya E. Silina, Lisann Mueller, Jayoung Park
Summary: In this study, filamentous bacteriophages were used to identify highly selective Cu(II) binding peptides using phage display technique. The engineered viruses showed strong Cu(II) binding potential, and a biosensor system based on AuNP-peptide was developed for sensitive and selective detection of Cu(II), achieving a detection limit lower than the WHO's accepted guideline.
Article
Microbiology
Andrzej Piekarowicz, Aneta Klyz, Daniel C. Stein
Summary: In this study, phagemid particles based on the Neisseria gonorrhoeae filamentous phage were used as a vaccine delivery system. The particles incorporated host proteins encoded by chromosomal genes or plasmids, and were prepared from three types of cells. The particles elicited a large amount of specific antibodies that recognized bacterial host cells and proteins, indicating the potential of filamentous phage as a novel immunogen delivery system.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Makoto Nakakido, Naoki Tanaka, Ayako Shimojo, Nobuhiro Miyamae, Kouhei Tsumoto
Summary: Respiratory infectious diseases pose a serious global threat, and novel antiviral materials are in high demand. In this study, a new platform was constructed to evaluate antiviral activity of surface coating materials using the M13 bacteriophage and phagemid system. The experimental system developed in this study provides rapid and effective screening, which can be applied to the development of novel antiviral materials.
Article
Engineering, Biomedical
Xue Dong, Pei Pan, Jing-Jie Ye, Qiu-Ling Zhang, Xian-Zheng Zhang
Summary: The study demonstrates a bioactive vaccine platform using hybrid M13 phage and personal tumor antigens for efficient delivery of antigens and activation of immune response. The vaccine not only prevents tumor occurrence but also delays tumor growth, showing potential synergy with immune checkpoint blockade therapy.
Article
Biochemistry & Molecular Biology
Ibukun Elizabeth Osadare, Ling Xiong, Ignacio Rubio, Ute Neugebauer, Adrian T. Press, Anuradha Ramoji, Juergen Popp
Summary: Sepsis is a life-threatening condition caused by an excessive and disproportionate immune response to an infection. This study used Raman spectroscopy to analyze the changes in splenic T-lymphocyte profiles during bacterial sepsis and hyperinflammatory endotoxemia. The results showed significant changes in DNA region of splenocytes during sepsis, indicating notable transcriptomic activity. Additionally, splenocytes exposed to high-dose lipopolysaccharide injection displayed significant protein and lipid profile changes, with variations in inflammation severity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Virology
Valery A. Petrenko, James W. Gillespie, Laura Maria De Plano, Michael A. Shokhen
Summary: This article introduces the use of recombinant filamentous bacteriophages to study the adaptation mechanisms of the SARS-CoV-2 virus to human host cells. By displaying mimotopes on the surface of bacteriophages, the structure of receptor-binding sites on the viral spike protein can be mimicked, allowing for the study of molecular mechanisms of virus infectivity.
Review
Chemistry, Physical
Carmelo Corsaro, Giulia Neri, Antonio Santoro, Enza Fazio
Summary: This article introduces the advantages and challenges of acrylate and methacrylate polymers, focusing on their applications in medical, electronic, food packaging, and environmental remediation fields. It also discusses the issue of their recyclability and describes the most influential strategies for recyclable composites based on acrylic polymers.
Article
Chemistry, Multidisciplinary
Laura Maria De Plano, Letteria Silipigni, Lorenzo Torrisi, Alfio Torrisi, Mariapompea Cutroneo, Vladimir Havranek, Anna Mackova, Vincenzo Zammuto, Concetta Gugliandolo, Maria Giovanna Rizzo, Salvatore P. P. Guglielmino, Domenico Franco
Summary: The helical geometry of virus capsid allows simple self-assembly of protein subunits, but small variations in subunits can affect the stability of the whole phage. In this study, the resistance to proton beam exposure of different phage clones was determined, and it was found that clones containing amino acids favorable to carbonylation showed the highest reduction in phage titer, while clones containing amino acids unfavorable to carbonylation showed the lowest reduction.
APPLIED SCIENCES-BASEL
(2022)
Article
Chemistry, Physical
Marcello Condorelli, Antonio Speciale, Francesco Cimino, Claudia Muscara, Enza Fazio, Luisa D'Urso, Carmelo Corsaro, Giulia Neri, Angela Maria Mezzasalma, Giuseppe Compagnini, Fortunato Neri, Antonina Saija
Summary: Gold nanoparticles (Au NPs) are highly regarded for their biocompatible nature and extensive biomedical applications. In our research, we investigated the bioproperties of functionalized Au NPs using LCCs for the first time. Results showed that the Au@LCC samples significantly reduced TNF gene expression and induced higher levels of heme oxygenase-1 gene expression compared to the 1:1 dispersion.
Article
Chemistry, Multidisciplinary
Giuseppe Nocito, Emanuele Luigi Sciuto, Domenico Franco, Francesco Nastasi, Luca Pulvirenti, Salvatore Petralia, Corrado Spinella, Giovanna Calabrese, Salvatore Guglielmino, Sabrina Conoci
Summary: Carbon dots (CDs), a type of carbon nanomaterials, have been synthesized from olive solid wastes and their properties and antibacterial activity have been evaluated. The study found that the CDs had a quasi-spherical shape, with different sizes for different regions. They exhibited absorption and photoluminescence characteristics, and higher antibacterial activity against Gram-positive bacteria. The surface charge of the CDs was found to influence their antibacterial properties.
Article
Chemistry, Multidisciplinary
Carmelo Corsaro, Sebastiano Vasi, Fortunato Neri, Angela Maria Mezzasalma, Giulia Neri, Enza Fazio
Summary: NMR measurements combined with chemometrics can be used to identify potential biomarkers and have various applications in different fields. Metabolomics, as a fingerprint of a sample, can benefit from statistical tools, and the current challenge is to enhance predictive power over large amounts of data using self-learning techniques.
APPLIED SCIENCES-BASEL
(2022)
Review
Biochemistry & Molecular Biology
Nunzio Iraci, Carmelo Corsaro, Salvatore Giofre, Giulia Neri, Angela Maria Mezzasalma, Martina Vacalebre, Antonio Speciale, Antonina Saija, Francesco Cimino, Enza Fazio
Summary: In recent years, there has been an increasing focus on developing new antiviral products from artificial synthesis processes using nanomaterials and natural matrices. Advanced computational approaches have also played a crucial role in repurposing therapeutics and reducing the time required for new drug discovery, especially during pandemics.
Review
Microbiology
Domenico Franco, Giovanna Calabrese, Salvatore Pietro Paolo Guglielmino, Sabrina Conoci
Summary: The increasing prevalence of antibiotic-resistant bacteria has led to a search for alternative antibacterial agents. Nanoparticles have emerged as a promising alternative due to their intrinsic antibacterial activity. However, the mechanisms underlying their action are still not well understood, limiting their use in clinical applications. This review provides an overview of the mechanisms underlying the antibacterial activity of metal and metal oxide nanoparticles, as well as the strategies employed by bacteria to develop resistance. Additionally, recent biomedical applications of these nanoparticles with antibacterial activity are discussed.
Article
Biochemistry & Molecular Biology
Valeriy V. Ginzburg, Enza Fazio, Carmelo Corsaro
Summary: In this study, a new dynamic mean-field model combined with the Sanchez-Lacombe thermodynamic theory was used to describe the equation of state and diffusivity of liquid water. The model predictions were consistent with experimental data.
Article
Chemistry, Physical
Carmelo Corsaro, Marcello Condorelli, Antonio Speciale, Francesco Cimino, Giuseppe Forte, Francesco Barreca, Salvatore Spadaro, Claudia Muscara, Manuela D'Arrigo, Giovanni Toscano, Luisa D'Urso, Giuseppe Compagnini, Fortunato Neri, Antonina Saija, Enza Fazio
Summary: The synthesis of contaminant-free silver@linear carbon chains (Ag@LCCs) nanohybrid systems, at different Ag/LCCs ratios, by pulsed laser ablation was investigated. The ablation products were characterized using UV-Vis optical absorption, micro-Raman spectroscopy, and transmission mode scanning electron microscopy. The experimental results were supported by theoretical simulations. Moreover, the bioproperties of the Ag@LCCs nanosystems were studied for wound-healing activity, and it was found that Ag@LCC nanohybrids exhibited good antibacterial properties and had enhanced interaction with mammalian cells, suggesting their potential application in wound healing.
Article
Chemistry, Physical
Ettore Maggiore, Carmelo Corsaro, Enza Fazio, Inam Mirza, Francesco Ripamonti, Matteo Tommasini, Paolo M. M. Ossi
Summary: This study aimed to replace the soft Ultra High Molecular Weight Polyethylene (UHMWPE) used for skiing bases with a hard metallic material. By using ultra-short laser pulses, the surface of square plates made of AISI 301H stainless steel was modified to reduce the friction coefficient. The treated bases performed worse than UHMWPE in laboratory tests, but waxing improved their performance.
Review
Polymer Science
Martina Vacalebre, Renato Frison, Carmelo Corsaro, Fortunato Neri, Antonio Santoro, Sabrina Conoci, Elena Anastasi, Maria Cristina Curatolo, Enza Fazio
Summary: Intraocular lenses (IOLs) are implanted after cataract surgery and various materials like collamer, hydrophobic acrylic, hydrophilic acrylic, PHEMA copolymer, PMMA, and silicone are used for IOL manufacturing. The evolution of polymeric materials for IOL design and their unique properties are discussed, including the need for biocompatibility and tunable refractive index to prevent posterior capsular opacification.
Article
Engineering, Electrical & Electronic
Rayhane Zribi, Angelo Ferlazzo, Enza Fazio, Marcello Condorelli, Luisa D'Urso, Giulia Neri, Carmelo Corsaro, Fortunato Neri, Giuseppe Compagnini, Giovanni Neri
Summary: This study fabricated and characterized a sensor based on silver nanoplates colloidal solutions deposited onto screen-printed carbon electrodes. The sensor showed wavelength-dependent and size-tunable electrochemical sensing response towards hydrogen peroxide (H2O2). The study also demonstrated the potential of using Ag nanoparticles with specific nanoplates morphology for electrochemical redox probes.
IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT
(2023)
Review
Biochemistry & Molecular Biology
Laura M. De Plano, Giovanna Calabrese, Sabrina Conoci, Salvatore P. P. Guglielmino, Salvatore Oddo, Antonella Caccamo
Summary: Alzheimer's disease, Parkinson's disease, amyotrophic lateral sclerosis, and Huntington's disease are prevalent neurodegenerative disorders affecting millions of people worldwide, with a lack of effective treatments. The CRISPR-Cas9 gene editing system has been widely applied in these diseases, showing great potential and versatility.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
