期刊
JOURNAL OF HAZARDOUS MATERIALS
卷 373, 期 -, 页码 483-492出版社
ELSEVIER
DOI: 10.1016/j.jhazmat.2019.03.082
关键词
Paper-based algal biosensor; Chlamydomonas reinhardtii; Chlorophyll fluorescence; Nanoencapsulated-atrazine; Sustainable agriculture
资金
- Sao Paulo Science Foundation (FAPESP)
- NanoSWS project [EraNetMed -RQ3-2016]
In this study we reported the development of a paper-based algal biosensor for the optical detection of nanoencapsulated-atrazine, a forefront nanoformulated herbicide with a high effective post-emergence herbicidal activity. In particular, the unicellular green photosynthetic algae Chlamydomonas reinhardtii was immobilised on a paper substrate soaked with an agar thin film and placed in a glass optical measurement cell, obtaining a totally environmental-friendly device. Nanoencapsulated-atrazine was detected by following the variable fluorescence (1-V-J) parameter, which decreased inversely proportional to the herbicide concentrations, in a range between 0.5 and 200 nM, indicating a linear relationship in the measured dose-response curves and a detection limit of 4 pM. Interference studies resulted in a very slight interference in presence of 2 ppm copper and 10 ppb arsenic at safety limits, as well as a slight matrix effect and a satisfactory recovery value of 96 +/- 5% for 75 nM nanoencapsulated-atrazine in tap water. Stability studies were also performed obtaining a good storage stability up to 3 weeks. Results demonstrated the suitability of the proposed paper-based optical biosensor as a valid support in smart agriculture for on site, environmental friendly, cost effective and sensitive nanoencapsulated-atrazine analysis.
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