期刊
JOURNAL OF FISH BIOLOGY
卷 94, 期 3, 页码 512-516出版社
WILEY
DOI: 10.1111/jfb.13919
关键词
cytochrome c oxidase subunit 1; endangered elasmobranchs; molecular markers; non-lethal methods
资金
- Sao Paulo Research Foundation (FAPESP) [2011/18513-9, 2013/08675-7, 2017/02420-8]
- National Council for Scientific and Technological Development (CNPq) [308430/2015-8]
- CNPq
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [13/08675-7] Funding Source: FAPESP
In this study we tested the use of mucus from five species of Neotropical marine batoid elasmobranchs to extract genomic DNA for barcoding and phylogenetic analysis. The DNA from all individuals sampled was successfully amplified and sequenced for molecular barcode, allowing 99-100% accuracy to the species level. This method proved to provide reliable and good-quality DNA for barcoding and phylogenetic analysis of Neotropical elasmobranchs, through rapid handling and with low disturbance to animals.
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