Article
Veterinary Sciences
Bokyeong Ryu, Mi-Young Son, Kwang Bo Jung, Ukjin Kim, Jin Kim, Ohman Kwon, Ye Seul Son, Cho-Rok Jung, Jae-Hak Park, C-Yoon Kim
Summary: This study established hESC-ELCs as an intestinal toxicity model, showing a stronger correlation with the in vivo model compared to immortalized cell lines. This suggests that hESC-ELCs could serve as the next-generation model for intestinal toxicity assessment.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Biochemical Research Methods
Lizandra Jimenez, Victor Stolzenbach, Patricia M. M. Ozawa, Marisol Ramirez-Solano, Qi Liu, Julien Sage, Alissa M. Weaver
Summary: This study found that small extracellular vesicles (SEVs) secreted by non-neuroendocrine cells in small cell lung cancer (SCLC) promote the adhesion and survival of neuroendocrine cells. Proteomic analysis revealed the enrichment of fibronectin, laminin 411, and laminin 511 in these SEVs.
Article
Biochemistry & Molecular Biology
Yujuan Jin, Tian Xiao, Yan Feng, Jinhua Yang, Chenchen Guo, Liang Hu, Hongbin Ji
Summary: The study identified heterogeneous subpopulations within non-NE SCLC cells, with Mes cells showing increased metastatic capability compared to Epi cells. The TGF-beta signaling pathway plays a crucial role in promoting SCLC metastasis in these cells.
JOURNAL OF GENETICS AND GENOMICS
(2021)
Article
Medicine, General & Internal
Xiuya Li, Fei Gao, Xiaochen Wang, Qianqian Liang, Aobing Bai, Zhuo Liu, Xinyun Chen, Ermin Li, Sifeng Chen, Chao Lu, Ruizhe Qian, Ning Sun, Ping Liang, Chen Xu
Summary: Knockout of the transcription factor E2A increased the proportion of nodal-like cells in hESC-derived CMs, indicating a potential role of E2A in enhancing the efficiency of generating sinoatrial node-like cardiomyocytes from human pluripotent stem cells in the future.
Article
Cell & Tissue Engineering
Qun Liu, Youkun Bi, Shaole Song, Keqi Zhu, Xinlong Qiao, Huiwen Wang, Guangju Ji
Summary: This study found that administration of human embryonic stem cell-derived exosomes can significantly alleviate pulmonary fibrosis and regulate inflammation and fibrosis through the miR-17-5p/Thbs2 axis. These findings provide new hope for the treatment of fibrosis-associated diseases.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Oncology
Borja Sese, Sandra Iniguez-Munoz, Miquel Ensenyat-Mendez, Pere Llinas-Arias, Guillem Ramis, Javier I. J. Orozco, Silvia Fernandez de Mattos, Priam Villalonga, Diego M. Marzese
Summary: This study identifies a subpopulation of glioma stem cells (GSCs), known as core-GSCs (c-GSCs), in glioblastoma (GBM) patients that have a similar profile to embryonic stem cells (ESCs) and downregulated immune-associated pathways. The development of an induced c-GSC (ic-GSC) cellular model provides insights into the immune evasion mechanisms in GBM and potential targets for improving immunotherapy treatments.
Article
Multidisciplinary Sciences
Yu Jin Jang, Mijeong Kim, Bum-Kyu Lee, Jonghwan Kim
Summary: The authors developed a simple procedure to convert human primed pluripotent stem cells into trophoblast stem-like cells using bone morphogenetic protein 4, providing an important opportunity to study human placenta development and pathology.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell & Tissue Engineering
Swati Mishra, Jasin Taelman, Mina Popovic, Laurentijn Tilleman, Evi Duthoo, Margot van der Jeught, Dieter Deforce, Filip van Nieuwerburgh, Bjoern Menten, Petra de Sutter, Annekatrien Boel, Susana M. Chuva De Sousa Lopes, Bjorn Heindryckx
Summary: This study found that continuous supplementation of ActA during differentiation of hESCs can promote differentiation into PGCLCs, and conversion to the 4i-state is beneficial for this process. The obtained PGCLCs may further differentiate into mature germ cells.
Article
Cell Biology
Xiaoling Xie, Xiaoling Zhou, Tingdang Liu, Zhiqian Zhong, Qi Zhou, Waqas Iqbal, Qingdong Xie, Chiju Wei, Xin Zhang, Thomas Ming Swi Chang, Pingnan Sun
Summary: In this study, a novel 3D differentiation system using alginate microcapsules was developed to differentiate hESCs into hepatocyte-like cells (hESC-Heps). The 3D hESC-Heps showed improved maturity and hepatic functions compared to 2D hESC-Heps. The 3D hESC-Heps expressed higher levels of hepatocyte-enriched genes and had better capability to metabolize indocyanine green and scavenge ammonia. Additionally, the 3D sodium alginate hydrogel microspheres protected hESC-Heps from viral infection.
Article
Cell & Tissue Engineering
Elisa Cuevas, Daniel L. Holder, Ashwak H. Alshehri, Julie Treguier, Jorn Lakowski, Jane C. Sowden
Summary: The study utilized CRISPR/Cas9 gene editing to create NRL-deficient embryonic stem cell (ESC) line, which differentiated into retinal organoids. The research found that in NRL-deficient organoids, photoreceptor development exhibited abnormalities, indicating a conserved molecular switch mechanism.
Article
Cell Biology
Bowen Zhang, Xumin Wu, Guicheng Zi, Lijuan He, Sihan Wang, Lin Chen, Zeng Fan, Xue Nan, Jiafei Xi, Wen Yue, Lei Wang, Liu Wang, Jie Hao, Xuetao Pei, Yanhua Li
Summary: By optimizing the culture protocol, a large number of megakaryocytes were successfully generated from human embryonic stem cells, confirming their biosafety and demonstrating their ability to form platelets. This study further enhances the clinical applications of megakaryocytes or platelets derived from pluripotent stem cells.
CELL PROLIFERATION
(2021)
Article
Engineering, Biomedical
Chao Sun, Yinhua Dong, Jun Wei, Meng Cai, Dongfang Liang, Yongqing Fu, You Zhou, Yi Sui, Fangda Wu, Roman Mikhaylov, Hanlin Wang, Feifei Fan, Zhihua Xie, Mercedes Stringer, Zhiyong Yang, Zhenlin Wu, Liangfei Tian, Xin Yang
Summary: This study develops a highly effective acoustic stimulator working at -20 MHz to investigate the roles of acousto-mechanical stimuli in the differentiation of hESCs. Our results show that acoustic dose alters the expression of extracellular matrix and physiological activity-related genes, indicating that acoustic stimulation is an important tool in regulating the stem cells' differentiation processes in cell therapy.
ACTA BIOMATERIALIA
(2022)
Article
Engineering, Biomedical
Tingting Gao, Xiyuan Zhao, Jie Hao, Yao Tian, Huike Ma, Wenjing Liu, Bin An, Faguo Sun, Shasha Liu, Baojie Guo, Shuaishuai Niu, Zhongwen Li, Chenxin Wang, Yukai Wang, Guihai Feng, Liu Wang, Wei Li, Jun Wu, Meijin Guo, Qi Zhou, Qi Gu
Summary: In this study, a new microcarrier with hyaluronic acid (HA) as the core material was developed for scalable serum-free suspension culture of hESC-derived MSCs. The optimal microcarriers, with a coating collagen concentration of 100 μg/mL or concave-structured surface (cHAMCs), allowed for the expansion of IMRCs within six days with a high yield. The harvested cells showed high viability, immunomodulatory and regenerative therapeutic promise, as well as increased secretion of extracellular matrix (ECM).
MATERIALS TODAY BIO
(2023)
Article
Developmental Biology
Andrea L. Bredemeyer, Junedh M. Amrute, Andrew L. Koenig, Rachel A. Idol, Li He, Stephanie A. Luff, Carissa Dege, Jamison M. Leid, Joel D. Schilling, J. Travis Hinson, Mary C. Dinauer, Christopher M. Sturgeon, Kory J. Lavine
Summary: Tissue-resident macrophages play a crucial role in organ homeostasis, tissue repair, remodeling, and regeneration. Researchers have developed a system to generate macrophages that resemble tissue-resident or monocyte-derived subsets, allowing for the study of their function and potential applications in tissue engineering and regenerative medicine.
Article
Biochemistry & Molecular Biology
Sang-Hoon Yoon, Mi-Rae Bae, Hyeonwoo La, Hyuk Song, Kwonho Hong, Jeong-Tae Do
Summary: This study proposed an efficient method for deriving NSCs from mouse ESCs through a combination of 3D culture for early neural lineage commitment and 2D NSC derivation. The results demonstrated that ESCs could efficiently differentiate into tripotent NSCs through specification in 3D culture followed by 2D culture.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)