Article
Biochemistry & Molecular Biology
Yulin Sun, Hongjuan Wang, Huan Chen, Sen Zhang, Jun Li, Jingni Zhang, Jianlu Tian, Youyu Zhang, Hongwei Hou, Qingyuan Hu
Summary: This study investigated the effects of nicotine on the cytotoxicity and genotoxicity of NNK using BEAS-2B cells expressing CYP2A13. The results showed that CYP2A13 caused cytotoxicity and genotoxicity of NNK in BEAS-2B cells, and the addition of nicotine could inhibit the toxicity of NNK.
Article
Chemistry, Physical
Giuseppina Nocca, Giuseppe D'Avenio, Adriana Amalfitano, Laura Chronopoulou, Alvaro Mordente, Cleofe Palocci, Mauro Grigioni
Summary: In this study, core-shell NPs loaded with GA were efficiently developed to enhance the drug's bioavailability in cells. The toxic effects of different forms of GA on HepG2 cells were evaluated using MTT and ECIS tests, highlighting the importance of real-time monitoring for studying bioactive substances in cell cultures.
Article
Chemistry, Analytical
Yong-Hyun Kim, Min-Seok Kim
Summary: This study developed a standardized method for preparing whole cigarette smoke condensate (WCSC) to obtain consistent quality WCSC. The effectiveness of the method was verified by evaluating the concentrations of total particulate matter (TPM) and organic compounds (OCs) in the samples at each step of WCSC preparation from cigarette smoke.
MICROCHEMICAL JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
A. M. Thasweer, P. Renuka Devi, Velusamy Thirunavukkarasu
Summary: Smoking is a major global health problem, and it is crucial to understand the mechanism of action of tobacco constituents on cellular processes. Through molecular docking and dynamics simulations, this study identified that NNK exhibited the highest affinity with α7nAChR and α4β2nAChR, while nicotine and NNN showed more stable interactions with different receptors. These findings can contribute to the development of effective nAChR agonists for treating neurological disorders and smoke addiction, ultimately improving health standards.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Review
Engineering, Electrical & Electronic
Ioana Voiculescu, Fang Li, Anis Nurashikin Nordin
Summary: The ECIS technique is a powerful and effective tool for real-time monitoring of cell behavior, with applications ranging from impedance of cancer cells and toxicity studies to analysis of beating cardiomyocytes. It offers advantages over conventional assays and provides opportunities for innovative research in the field of mammalian cell properties and physiological functions.
IEEE SENSORS JOURNAL
(2021)
Article
Chemistry, Multidisciplinary
Mina Eghbal, Martin Rozman, Veno Kononenko, Matej Hacevar, Damjana Drobne
Summary: An electrochemical biosensor based on cells was used to detect the cytotoxic effects of Zn2+ ions on A549 cells, showing potential as a low-cost detector for various chemicals and nanomaterials. The biosensor successfully identified changes in cell morphology and attachment patterns through ECIS measurements combined with cytotoxicity tests and microscopic observation.
Article
Biochemistry & Molecular Biology
Klaudia Kowalik, Magdalena Polak-Berecka, Monika Prendecka-Wrobel, Dominika Pigon-Zajac, Iwona Niedzwiedz, Dominik Szwajgier, Ewa Baranowska-Wojcik, Adam Wasko
Summary: This study examined the biological activity of an in vitro digested infusion of Epilobium angustifolium (fireweed) in a model system of intestinal epithelial and colon cancer tissues. The results showed that the infusion inhibited the growth of intestinal cancer cells HT-29, which can be attributed to the activity of quercetin and kaempferol. The cytotoxic effect of the infusion was dose-dependent, and it did not affect the growth of beneficial intestinal bacteria but significantly inhibited E. coli.
Article
Environmental Sciences
Sadiya Bi Shaikh, Wai Cheung Tung, Cortney Pang, Joseph Lucas, Dongmei Li, Irfan Rahman
Summary: Oral nicotine pouches (ONPs) are a modern form of smokeless tobacco products. Flavored ONPs may cause adverse effects on oral and respiratory epithelial cells, leading to inflammation and toxicological responses.
Article
Chemistry, Analytical
Chen Zhou, Sebastian Bette, Aaron Babendreyer, Christina Hoffmann, Sven Gerlach, Tom Kremers, Andreas Ludwig, Bernd Hoffmann, Rudolf Merkel, Stefan Uhlig, Uwe Schnakenberg
Summary: The development of a cell-stretching platform utilizing stretchable microelectrodes and an ultra-elastic substrate allows for up to 35% cyclic stretch. The novel interlaced meander design ensures reliable electrical contacting of the microelectrodes under stretch, enabling label-free, real-time monitoring of cell monolayers. Proof-of-concept experiments show that the system can discern impedance changes in different epithelial cell types, providing a potential tool for continuous monitoring of cell monolayers under strain for various applications.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Medicine, Legal
Selvin H. Edwards, Matthew D. Hassink, Kenneth M. Taylor, An T. Vu
Summary: Smokeless tobacco products contain addictive and carcinogenic substances that can cause nicotine dependence and oral cancers. This study measured the levels of various substances in 16 smokeless tobacco products and found significant variations. Additionally, the study observed that the analytical variability stabilized after seven replicate measurements.
REGULATORY TOXICOLOGY AND PHARMACOLOGY
(2022)
Article
Chemistry, Analytical
Fuentes-Velez Susana, Fagoonee Sharmila, Sanginario Alessandro, Gallo Valentina, Riganti Chiara, Pizzi Marco, Altruda Fiorella, Demarchi Danilo
Summary: Interest in impedance-based cellular assays is growing due to their multiple advantages, and our study demonstrates the potential of ECIS in cancer treatment decisions and early detection of chemoresistance. The analysis of impedance-based curves distinguishes between chemo-sensitive and chemo-resistant cells, and promises the study of time-dependent factors related to resistance acquisition in cells. The continuous monitoring and highthroughput early detection of drug resistance using the ECIS device presents new possibilities for cancer treatment and drug screening.
Article
Chemistry, Analytical
Zheyuan Zhang, Xichen Yuan, Huijie Guo, Peng Shang
Summary: Detection sensitivity is crucial in ECIS sensor application, and electrode configuration directly affects sensitivity. This study fabricated ECIS sensor arrays with various electrode configurations to monitor living MC3T3-E1 cells. The results showed that smaller and fewer electrodes with a diameter larger than 25 μm had higher impedance values and generated more impedance shift to cell status change. However, when the electrode diameter was smaller, there was no clear relationship between impedance and membrane capacitance shift to cell status change and electrode geometry. The proposed sensor chip provides a basis for selecting electrode configuration of monopolar electrodes and enables sustained and stable detection of cellular impedance.
Article
Chemistry, Multidisciplinary
Andrzej Kociubinski, Aleksandra Wilczynska, Pawel A. Mazurek, Dominika Pigon-Zajac, Teresa Malecka-Massalska, Monika Prendecka-Wrobel
Summary: This paper explores the possibility of using impedance measurement to monitor morphological changes in cell culture in the presence of an electromagnetic field. The study used Electric Cell-Substrate Impedance Sensing (ECIS) method to study cell behaviors. A device that could be connected in a climatic chamber was used to test mouse fibroblast culture. The results showed little effect of the electromagnetic field on cell culture, and the observed changes in impedance, resistance, and capacitance were likely due to the separation of positive and negative ions in the field.
APPLIED SCIENCES-BASEL
(2023)
Article
Biochemistry & Molecular Biology
Nadja Engel, Michael Dau, Vivien Engel, Denise Franz, Fabian Klemmstein, Christiane Thanisch, Juergen F. Kolb, Marcus Frank, Armin Springer, Ruediger Koehling, Rainer Bader, Bernhard Frerich, Nadine Wiesmann, Diana Heimes, Peer W. Kaemmerer
Summary: By combining electrical stimulation of a commercial titanium implant with real-time impedance sensing, osteogenesis progress can be monitored and electrical stimulation can be adjusted accordingly for individual fracture healing.
Article
Physiology
Zhi Tian, Jendayi Dixon, Xiaofang Guo, Benjamin Deal, Qianjin Liao, Yujuan Zhou, Feng Cheng, Diane S. Allen-Gipson
Summary: This study showed that long-term exposure to cigarette smoke can lead to lung injury, promoting adenosine production and ADORA2B receptor activation, inhibiting PKC alpha activity and p-ERK signaling pathway, thereby impeding airway wound repair. Knocking down CD73 and ADORA2B receptors can significantly improve airway injury.
FRONTIERS IN PHYSIOLOGY
(2021)